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Particuology 2024年第1期84卷 168-177页

作者： Qingye Meng Shuangling Zhong Jingfei Wang zhenqian zhang Yan Gao Xuejun Cui College of Chemistry Jilin University Changchun130012China College of Resources and Environment Jilin Agricultural University Changchun130118China State Key Laboratory of Supramolecular Structure and Materials Jilin UniversityChangchun130012China Weihai Institute for Bionics-Jlin University Weihai264400China

In this study,sodium alginate(SA),a non-toxic natural polysaccharide with good biocompatibility and biodegradability,was developed for targeted delivery of curcumin(CUR)in tumor therapy.The strategy is to sulfhydrylate the folic acid(FA)modified SA,and the CUR dissolved in ethyl acetate(EAC)phase is coated in microcapsules by a quick,efficient and environment-friendly sonochemical method.The EAC in the microcapsule core is volatile,which can be recycled and reused to reduce cost.The prepared mi-crocapsules(FA-RSMCs@CUR)exhibited similar toxicity to free curcumin in anti-tumour evaluation in vitro.FA-RSMCs@CUR also exhibited effective antibacterial properties in the antibacterial evaluation in vitro.It is expected to become a low-cost tumor targeting vector in the future,and has the potential to be promoted in clinical application.

关键词： Reductive-responsive Sodium alginate Microcapsule Targeted delivery Sonochemical

Morphological determination of localization and function of Golgi proteins

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Biophysics Reports 2024年第2期10卷 121-132页

作者： Yusheng Xing Yannan Jian Xiaodan Zhao Yue zhang zhenqian zhang Xing zhang Xiaoyan zhang College of Biomedicine and Health College of Life Science and Technology Huazhong Agricultural University

In animal cells, the Golgi apparatus serves as the central hub of the endomembrane secretory pathway.It is responsible for the processing, modification, and sorting of proteins and lipids. The unique stacking and ribbon-like architecture of the Golgi apparatus forms the foundation for its precise functionality. Under cellular stress or pathological conditions, the structure of the Golgi and its important glycosylation modification function may change. It is crucial to employ suitable methodologies to study the structure and function of the Golgi apparatus, particularly when assessing the involvement of a target protein in Golgi regulation. This article provides a comprehensive overview of the diverse microscopy techniques used to determine the specific location of the target protein within the Golgi apparatus. Additionally, it outlines methods for assessing changes in the Golgi structure and its glycosylation modification function following the knockout of the target gene.

关键词： Golgi apparatus Golgi fragmentation Immunofluorescence Super-resolution microscopy Electron microscopy Lectin staining Glycosylation

Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations

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Journal of Genetics and Genomics 2022年第12期49卷 1093-1100页

作者： zhenqian zhang Bin Zhou State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhouZhejiang 310024China School of Life Science and Technology Shanghai Tech UniversityShanghai 201210China

It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs:gCap(general capillary)and aCap(aerocyte).They are identified by two unique markers,respectively:plasmalemmal vesicle-associated protein(PLVAP)and carbonic anhydrase IV(CAR4).Here,we report two novel knock-in mouse lines Plvap-CreER and Car4-CreER,which genetically target gCap and aCap,respectively.Induced by tamoxifen,the Plvap-CreER and Car4-CreER alleles mediate specific and efficient Cre-loxP recombinations in PLVAP+gCap and CAR4+aCap,respectively,in the lungs.These two mouse lines are useful genetic tools to investigate cell fates and functions of PLVAP+and CAR4+cells in lung homeostasis,injury and repair.

关键词： lung homeostasis capillary

Magnetocaloric effect and slow magnetic relaxation behavior in binuclear rare earth based RE_(2)(L)_(2)(DMF)4(RE=Gd,Tb,and Dy) complexes

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Journal of Rare Earths 2022年第6期40卷 916-923,I0003页

作者： zhenqian zhang Yuwei Wu Haifeng Wang Lingwei Li Key Laboratory of Novel Materials for Sensor of Zhejiang Province Institute of Advanced Magnetic MaterialsHangzhou Dianzi UniversityHangzhou 310012China State Key Laboratory of Solidification Processing Center of Advanced Lubrication and Seal MaterialsNorthwestern Polytechnical UniversityXi'an 710072China Key Laboratory of Electromagnetic Processing of Materials(Ministry of Education) Northeastern UniversityShenyang 110819China

Three binuclear rare earth based complexes combining RE ions with semirigid tricarboxylic ligand(H_(3)L).namely,[RE_(2)(L)_(2)(DMF)_(4)][RE=Gd,Tb,and Dy;H_(3)L=5-((4-Carboxybenzyl)oxy)isophthalic acid;DMF=N,N-dimethylformamide]complexes,were fabricated success fully.The RE_(2)(L)_(2)(DMF)_(4) co mplexe s consist of two central RE ions with the same coordination environment which were connected by two tridentate bridging carboxylic groups and two syn-syn bidentate bridging carboxylic groups originating from the L^(3-)ligands to form the{RE_(2)}dimeric unit,and thus provides the basis for further constructing a dense three-dimensional(3 D)network structure.Moreover,the present RE_(2)(L)_(2)(DMF)_(4) complexes can be described by a topology diagram with the topology point symbol of{4^(2)·6}_(2){4^(4)·6^(2)·8^(7)·10^(2)}.Weak antiferromagnetic(AFM)coupling between the adjacent RE ions for all the present complexes was found according to the magnetic calculations.The observed significant cryogenic magnetocaloric effect(MCE)with the maximum magnetic entropy change-ΔS_(M)^(max) to be 26.3 J/(kg·K)withΔH=7 T in Gd_(2)(L)_(2)(DMF)_(4) complex makes it competitive for the cryogenic magnetic refrigerant.Moreover,the slow magnetic relaxation behavior at 0.2 T dc field with an obvious large U_(eff)/k=45(4)K and τ_(0)=6.5(2)×10^(-10)s was confirmed in Dy_(2)(L)_(2)(DMF)_(4)complex.This work not only provides an effective strategy for obtaining molecular materials with high MCE,but also confirms that tricarboxylate ligands are the ideal choice for constructing stable high dimensional geometric structures.

关键词： Magnetocaloric effect Rare earth based complex Magnetic properties Slow magnetic relaxation bahavior

Calcium-dependent protein kinases CPK21 and CPK23 phosphorylate and activate the iron-regulated transporter IRT1 to regulate iron deficiency in Arabidopsis

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Science China(Life Sciences) 2023年第11期66卷 2646-2662页

作者： zhangqing Wang Yanting zhang Yisong Liu Dali Fu zhang You Panpan Huang Huiling Gao zhenqian zhang Cun Wang State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences Northwest A&F UniversityYangling 712100China Institute of Future Agriculture Northwest Agriculture&Forestry UniversityYangling 712100China

Iron(Fe)is an essential micronutrient for all organisms.Fe availability in the soil is usually much lower than that required for plant growth,and Fe deficiencies seriously restrict crop growth and yield.Calcium(Ca2+)is a second messenger in all eukaryotes;however,it remains largely unknown how Ca2+regulates Fe deficiency.In this study,mutations in CPK21 and CPK23,which are two highly homologous calcium-dependent protein kinases,conferredimpaired growth and rootdevelopment under Fe-deficient conditions,whereas constitutively active CPK21 and CPK23 enhanced plant tolerance to Fe-deficient conditions.Furthermore,we found that CPK21 and CPK23 interacted with and phosphorylated the Fe transporter IRONREGULATED TRANSPORTER1(IRT1)at the Ser149 residue.Biochemical analyses and complementation of Fe transport in yeast and plants indicated that IRT1 Ser149 is critical for IRT1 transport activity.Taken together,these findings suggest that the CPK21/23-IRT1 signaling pathway is critical for Fe homeostasis in plants and provides targets for improving Fe-deficient environments and breeding crops resistant to Fe-deficient conditions.

关键词： Arabidopsis calcium-dependent protein kinases IRT1 iron

Ca^(2+)-dependent successive phosphorylation of vacuolar transporter MTP8 by CBL2/3-CIPK3/9/26 and CPK5 is critical for manganese homeostasis in Arabidopsis

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Molecular Plant 2022年第3期15卷 419-437页

作者： Chuanfeng Ju zhenqian zhang Jinping Deng Cuicui Miao zhangqing Wang Lukas Wallrad Laiba Javed Dali Fu Ting zhang Jörg Kudla Zhizhong Gong Cun Wang State Key Laboratory of Crop Stress Biology for Arid Areas College of Life Sciencesand Institute of Future AgricultureNorthwest Agriculture&Forestry UniversityYanglingShaanxi 712100China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Institut fur Biologie und Biotechnologie der Pflanzen Westfälische Wilhelms-Universität48149 MünsterGermany College of Life Sciences Hebei UniversityBaodingChina

Manganese(Mn)is an essential micronutrient for all living organisms.However,excess Mn supply that can occur in acid or waterlogged soils has toxic effects on plant physiology and development.Although a variety of Mn transporter families have been characterized,we have only a rudimentary understanding of how these transporters are regulated to uphold and adjust Mn homeostasis in plants.Here,we demonstrate that two calcineurin-B-like proteins,CBL2/3,and their interacting kinases,CIPK3/9/26,are key regulators of plant Mn homeostasis.Arabidopsis mutants lacking CBL2 and 3 or their interacting protein kinases CIPK3/9/26 exhibit remarkably high Mn tolerance.Intriguingly,CIPK3/9/26 interact with and phosphorylate the tonoplast-localized Mn and iron(Fe)transporter MTP8 primarily at Ser35,which is conserved among MTP8 proteins from various species.Mn transport complementation assays in yeast combined with multiple physiological assays indicate that CBL-CIPK-mediated phosphorylation of MTP8 negatively regulates its transport activity from the cytoplasm to the vacuole.Moreover,we show that sequential phosphorylation of MTP8,initially at Ser31/32 by the calcium-dependent protein kinase CPK5 and subsequently at Ser35 by CIPK26,provides an activation/deactivation fine-tuning mechanism for differential regulation of Mn transport.Collectively,our findings define a two-tiered calcium-controlled mechanism for dynamic regulation of Mn homeostasis under conditions of fluctuating Mn supply.

关键词： manganese Ca^(2+) calcineurin B-like proteins(CBLs) CBL-interacting protein kinases(CIPKs) Mn transporter MTP8 calcium-dependent protein kinases(CPKs)

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近代中国外来语入诗现象及其诗学意义

广东社会科学 2024年第1期 144-154页

作者：张振谦暨南大学中文系广州510632

在中国诗歌发展史上,以外来语入诗的创作现象,古已有之。及至近代,随着中外文化接触的日益频繁,不少诗人将英语音译词和日语汉字词植入旧体诗中,并往往采取自注形式加以解释。近代中国外来语入诗现象主要集中于以竹枝词为代表的古体诗,... 详细信息

在中国诗歌发展史上,以外来语入诗的创作现象,古已有之。及至近代,随着中外文化接触的日益频繁,不少诗人将英语音译词和日语汉字词植入旧体诗中,并往往采取自注形式加以解释。近代中国外来语入诗现象主要集中于以竹枝词为代表的古体诗,岭南和上海是其最活跃的创作地域。就跨文化视角而言,外来语入诗具有独特的诗学意义,它的出现不仅带来了新的思想文化,标志着一种新的诗学观念诞生,而且拓展了诗歌题材,具有建构异国形象的功能;同时促进了中国诗歌从旧到新的变化,对新诗创作产生了重要而深远的影响。

关键词：外来语入诗诗界革命竹枝词诗学意义

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宫观官制度与南宋前中期信州文人群体

长江学术 2024年第1期 61-72页

作者：张振谦暨南大学文学院广东广州510632

宫观官制度对宋代士人产生了重要影响,为他们的退居生活提供了基本保障,促进了地域文学的繁荣。南渡初期,吕本中与曾几先后领任宫观官寓居上饶,在他们的吸引下,信州一带的文人士大夫时常聚饮酬唱,形成了颇为闻名的地域文人群体。南宋中... 详细信息

宫观官制度对宋代士人产生了重要影响,为他们的退居生活提供了基本保障,促进了地域文学的繁荣。南渡初期,吕本中与曾几先后领任宫观官寓居上饶,在他们的吸引下,信州一带的文人士大夫时常聚饮酬唱,形成了颇为闻名的地域文人群体。南宋中期,赵蕃监南岳庙闲居玉山30余年,接续吕、曾倡导的文化精神和文学传统,与当地耆旧诗人往来频繁,成为信州文人群体的核心人物之一。与此同时,辛弃疾因罢官而主管冲佑观赋闲带湖和瓢泉长达20年,其信州诗词透露出闲逸自适与愤懑不平交织的心态,塑造了人生最后阶段的双重自我形象:赋闲归耕的“稼轩居士”和壮志未酬的爱国文士。

关键词：宫观官制度信州文人群体吕本中曾几赵蕃辛弃疾

Achievement of promising cryogenic magnetocaloric performances in La_(1-x)Pr_(x)Fe_(12)B_(6)compounds

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Journal of Materials Science & Technology 2021年第33期92卷 138-142页

作者： Zhipan Ma Xiaoshi Dong zhenqian zhang Lingwei Li Key Laboratory of Electromagnetic Processing of Materials(Ministry of Education) Northeastern UniversityShenyang 110819China Key Laboratory of Novel Materials for Sensor of Zhejiang Province Institute of Advanced Magnetic MaterialsHangzhou Dianzi UniversityHangzhou 310012China

The magnetic refrigeration(MR)utilizing magnetocaloric effect(MCE)has been recognized as an environmentally friendly and energy efficiency technology.Here we presented the magnetic properties and MCE in Pr-doped La_(1-x)Pr_(x)Fe_(12)B_(6)(x=0.05-0.2)itinerant-electron metamagnetic(IEM)compounds.A small amount of Pr doping La site can greatly improve the peak values in the magnetic entropy change S_(M)(T)curves,especially under relatively low magnetic field changes(ΔH).Additionally,the peak temperature increases gradually and the magnetic hysteresis reduces gradually with increasing x.The observed MCE in present La_(1-x)Pr_(x)Fe_(12)B_(6)compounds is related to its field-induced first-ordered IEM transition.The peak values ofΔS_(M)for La_(1-x)Pr_(x)Fe_(12)B_(6)compounds reach 13.4,15.4,12.5 and 13.0 J/(kg K)at T_(C)~58,68,72and 89 K for x=0.05,0.10,0.15 and 0.2 under H of 0-7 T,respectively.The corresponding relative cooling power values are 462.3,480.7,372.4 and 375.7 J/kg.The present La_(1-x)Pr_(x)Fe_(12)B_(6)compounds could be good candidates for active MR application if the magnetic and thermal hysteresis can be further reduced.The present work indicates that the La Fe_(12)B_(6)-based material system could also exhibit promising magnetocaloric performances.

关键词： Magnetocaloric effect(MCE) Rare earth La_(1-x)Pr_(x)Fe_(12)B_(6)compounds Magnetic properties

Development of the automatic navigation system for combine harvester based on GNSS

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International Journal of Agricultural and Biological Engineering 2021年第5期14卷 163-171页

作者： Shichao Li Man zhang Ruyue Cao Yuhan Ji zhenqian zhang Han Li Yanxin Yin Key Lab of Modern Precision Agriculture System Integration Research Ministry of EducationChina Agricultural UniversityBeijing 100083China National Research Center of Intelligent Equipment for Agriculture Beijing 100097China

An automatic navigation system was developed to realize automatic driving for combine harvester,including the mechanical design,control method and software design.First of all,for the harvester modified with the automatic navigation system,a dynamic calibration method of the rear wheel center position was proposed.The control part included the navigation controller and the steering controller.A variable universe fuzzy controller was designed to the navigation controller,which used fuzzy control to change the fuzzy universe of input and output dynamically,that means,under the condition that the fuzzy rules remain unchanged,the fuzzy universe changes with the change of input,which is an adaptive fuzzy control method and can modify the control strategy in time.To realize the automatic navigation of the harvester,the decision result of the navigation controller based on the variable universe fuzzy control was input into the steering controller,and then the electric steering wheel was controlled to rotate.To test the performance of the designed automatic navigation system,the field experiment was carried out.When the combine harvester was navigating linearly at a speed of 0.8 m/s,the overall root mean square error(RMSE)of the lateral deviation was 5.87 cm.The test results showed that the system was designed could make the combine track the preset path smoothly and stably,and the tracking accuracy was at the centimeter level.

关键词： automatic navigation system combine harvester GNSS development dynamic calibration variable universe adaptive fuzzy control