Generation of Plvap-CreER and Car4-CreER for genetic targeting of distinct lung capillary populations

作     者：Zhenqian Zhang Bin Zhou Zhenqian Zhang;Bin Zhou

作者机构：State Key Laboratory of Cell BiologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang ProvinceSchool of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhouZhejiang 310024China School of Life Science and TechnologyShanghai Tech UniversityShanghai 201210China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2022年第49卷第12期

页      面：1093-1100页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Key Research&Development Program of China(2019YFA0110403,2019YFA080200) the National Science Foundation of China(82088101,32050087,91849202,31730112) 

主　　题：lung homeostasis capillary 

摘      要：It has been reported recently that there are two distinct subpopulations of capillary endothelial cells in the mammalian lungs:gCap(general capillary)and aCap(aerocyte).They are identified by two unique markers,respectively:plasmalemmal vesicle-associated protein(PLVAP)and carbonic anhydrase IV(CAR4).Here,we report two novel knock-in mouse lines Plvap-CreER and Car4-CreER,which genetically target gCap and aCap,*** by tamoxifen,the Plvap-CreER and Car4-CreER alleles mediate specific and efficient Cre-loxP recombinations in PLVAP+gCap and CAR4+aCap,respectively,in the *** two mouse lines are useful genetic tools to investigate cell fates and functions of PLVAP+and CAR4+cells in lung homeostasis,injury and repair.