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Frontiers of Medicine 2013年第3期7卷 301-305页

作者： Tingting Wang Shanglong Yao zhengyuan xia Michael G.Irwin Department of Anesthesiology and Critical Care Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Anesthesiology The University of Hong KongHong Kong SARChina

Systemic inflammatory response following myocardial ischemia-reperfusion injury(IRI)to a specific organ may cause *** post-conditioning(IPostC)has emerged as a promising method for myocardial protection against IRI both in experimental and in clinical *** of endogenous nitric oxide(NO)is one of the major mechanisms by which IPostC confers ***,the sensitivity of the diabetic heart to IPostC is impaired and the underlying mechanism is ***(APN)is an adipocyte-derived plasma protein with anti-diabetic and anti-inflammatory *** levels of APN are decreased in obese subjects and in patients with type 2 *** supplementation has been shown to increase NO production and attenuate myocardial IRI in normal(non-diabetic)***,the effect of APN on myocardial injury in diabetic subjects,especially its potential in restoring the sensitivity of the diabetic heart to IPostC has not been *** the current paper,we discussed the possible reasons why the myocardium of diabetic subjects loses sensitivity to IPostC and also highlighted the potential effectiveness and mechanism of APN in restoring IPostC cardioprotection in *** review proposes to conduct studies that may facilitate the development of novel and optimal therapies to enhance cardioprotection in patients with severe diseases such as diabetes.

关键词： adiponectin ischemic post-conditioning ischemia reperfusion injury diabetes

Mesozoic Reservoir Predictionin the Longdong Loess Plateau

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Applied Geophysics 2004年第1期1卷 20-25页

作者： WangDaxing GaoJinghuai LiYouming xiazhengyuan WangBaojiang InstituteofGeologyandGeophysics ChineseAcademyofSciencesBeijing100029China. Xi＇anJiaotongUniversity Xi＇an710000China. ChangqingOilFieldCompany ChangqingXinglongyuanBlockXi＇an710021China.

This paper summarizes a set of interpretation technologies for Mesozoic sandstone reservoir prediction in the Longdong loess plateau, such as seismic sequence processing and interpretation based on generalized S transform, the eroded paleo-geomorphology interpretation of the top of the Triassic and a variety of lateral reservoir predictions. The effects of employing these technologies are compared and analyzed, as well. The research results show that seismic sequence processing interpretation technology based on generalized S transform can distinguish 3ms (about the thickness of 6 m)sequence interface. Consequently the technology can ascertain the distribution of a sand body of the formation Ch 8 and expand the exploration area of the Xifeng oil field in the Longdong area.

关键词： seismic sandstone reservoir lateral prediction and reserves

Protective effect of ginsenoside Rb1 against ischemia/reperfusion injury in streptozotocin-induced diabetic rats

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Protective effect of ginsenoside Rb1 against ischemia/reperf...

Downregulation of cardiac PIASy inhibits Cx43 SUMOylation and ameliorates ventricular arrhythmias in a rat model of myocardial ischemia/reperfusion injury

第十五次长江流域麻醉学学术年会暨2010年中南六省麻醉学学术年会暨2010年湖北省麻醉学学术年会

作者： Yang Wu,Zhong-yuan xia,zhengyuan xia,Li Zhang,Juan Dou,Jinjin Xu,Bo Zhao

OBJECTIVE:Diabetic patients with cardiovascular disease have a high mortality rate than patients without diabetes. The cardioprotective effect of ginsenoside Rb1 present in ginseng on myocardial ischemia reperfusion (I/R) has not been examined in diabetic myocardium. The present study was designed to investigate whether ginsenoside Rb1 can protect the heart against I/R injury in diabetes models and search the underlying mechanism. METHODS:Ginsenoside Rb1,wortmaninn were administered prior to I/R procedure. Rats were divided into 7 groups:(i) diabetic sham,(ii) normal,(iii) diabetic,(iv) diabetic+ginsenosid Rb1,(v) diabetic+wortmaninn,(vi) diabetic +ginsenoside Rb1+wortmaninn,(vii) diabetic sham+wortmaninn. After the treatment,hearts were subjected to 30 min of ischemia followed by 2 h of reperfusion. Arterial blood pressure,Heart rate were monitored throughout the whole procedure. Additionally,plasma creatine kinase isoenzyme MB (CK-MB) and lactate dehydrogenase (LDH) were analyzed to assess the degree of cardiac injury. Myocardial infarction,caspase-3 activity and myocardial apoptosis (TUNEL analysis) were also measured. RESULTS:Myocardial I/R caused significant cardiac dysfunction and myocardial apoptosis in diabetic rats. Diabetic group treated with ginsenoside Rb1 demonstrated significant improved cardiac function,reduced infarct size,cardiomyocyte apoptosis and caspase-3 activity compared to the diabetic group or the group treated with wortmaninn. Ginsenoside Rb1 significantly unregulated phosphorylated Akt (p-AKT) expression. Treatment with wortmaninn in ginsenoside Rb1-treated diabetic rats demonstrated significant downregulation of p-AKT. CONCLUSION:Ginsenoside Rb1 exerts cardioprotective effects against myocardial I/R injury in diabetic rats,including anti-cardiomyocyte apoptosis,which is partly through activation of PI3 kinase/Akt pathway. Thus this study shows a novel pharmacological preconditioning with ginsenoside Rb1 in the diabetic myocardium.

关键词： Diabetes · Ginseng · Ginsenoside Rb1 · Ischemia/reperfusion injury · Apoptosis · Akt

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Chinese Medical Journal 2023年第11期136卷 1349-1357页

作者： Tingting Wang Jinmin Liu Chenchen Hu Xin Wei Linlin Han Afang Zhu Rong Wang Zhijun Chen zhengyuan xia Shanglong Yao Weike Mao Department of Anesthesiology Institute of Anesthesiology and Critical Care MedicineUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Anesthesiology Wuhan No.1 HospitalWuhanHubei 430022China State Key Laboratory of Pharmaceutical Biotechnology Department of MedicineThe University of Hong KongHong KongChina

Background:Dysfunction of the gap junction channel protein connexin 43(Cx43)contributes to myocardial ischemia/reperfusion(I/R)-induced ventricular ***43 can be regulated by small ubiquitin-like modifier(SUMO)*** inhibitor of activated STAT Y(PIASy)is an E3 SUMO ligase for its target ***,whether Cx43 is a target protein of PIASy and whether Cx43 SUMOylation plays a role in I/R-induced arrhythmias are largely ***:Male Sprague-Dawley rats were infected with PIASy short hairpin ribonucleic acid(shRNA)using recombinant adeno-associated virus subtype 9(rAAV9).Two weeks later,the rats were subjected to 45 min of left coronary artery occlusion followed by 2 h *** was recorded to assess *** ventricular tissues were collected for molecular biological ***:Following 45 min of ischemia,QRS duration and QTc intervals statistically significantly increased,but these values decreased after transfecting PIASy *** downregulation ameliorated ventricular arrhythmias induced by myocardial I/R,as evidenced by the decreased incidence of ventricular tachycardia and ventricular fibrillation,and reduced arrythmia *** addition,myocardial I/R statistically significantly induced PIASy expression and Cx43 SUMOylation,accompanied by reduced Cx43 phosphorylation and plakophilin 2(PKP2)***,PIASy downregulation remarkably reduced Cx43 SUMOylation,accompanied by increased Cx43 phosphorylation and PKP2 expression after I/***:PIASy downregulation inhibited Cx43 SUMOylation and increased PKP2 expression,thereby improving ventricular arrhythmias in ischemic/reperfused rats heart.

关键词： Connexin 43 Myocardial reperfusion injury PIASy PKP2 SUMOylation Arrhythmias

别嘌呤醇通过调节自噬及恢复Nrf2通路减轻糖尿病心肌病

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武汉大学学报（医学版） 2019年第2期40卷 213-217页

作者：罗洁荣黄焕森夏正远广州市第一人民医院麻醉科广东广州510180 广州医科大学附属第二医院麻醉科广东广州510260

目的:探讨抗氧化药物别嘌呤醇(ALP)对糖尿病心肌病的保护作用及机制。方法:用高浓度的葡萄糖培养H9C2细胞建立糖尿病心肌病模型,实验分组为正常糖浓度培养组(NG),高糖组(HG)及高糖加ALP处理组(HG+ALP),检测细胞分泌乳酸脱氢酶活性,观察... 详细信息

目的:探讨抗氧化药物别嘌呤醇(ALP)对糖尿病心肌病的保护作用及机制。方法:用高浓度的葡萄糖培养H9C2细胞建立糖尿病心肌病模型,实验分组为正常糖浓度培养组(NG),高糖组(HG)及高糖加ALP处理组(HG+ALP),检测细胞分泌乳酸脱氢酶活性,观察细胞形态,用Western Blot和实时定量PCR分别检测Nrf2及LC3蛋白表达量和mRNA表达量。结果:HG组较NG组LDH释放量增多及细胞表面积增大(P<0. 05),ALP处理后可以改善上述指标(P<0. 05,HG+ALP vs HG);与此同时,HG组Nrf2的蛋白量相比NG组下降,而LC3Ⅱ/Ⅰ比值升高(P<0. 05),在给予ALP处理后,Nrf2及LC3Ⅱ/Ⅰ比值均恢复至正常水平(P<0. 05,HG+ALP vs HG),而Nrf2 mRNA在3组间均无统计学差异。结论:ALP通过调控自噬修复Nrf2保护通路发挥糖尿病心肌病的保护作用。

关键词：糖尿病心肌病 H9C2 别嘌呤醇细胞自噬

鄂尔多斯盆地上古生界含气砂岩体综合预测目标处理解释技术

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天然气工业 1998年第5期18卷 5-9页

作者：潘存焕郭忠铭王大兴夏正元长庆石油勘探局勘探开发研究院

鄂尔多斯盆地上古生界含气砂岩体主要属湖相三角洲平原分流河道沉积，具低孔低渗和高阻抗特征。针对这一地质目标，研制和总结出４类１５项方法技术，建立了鄂尔多斯盆地上古生界含气砂岩体综合预测目标处理解释技术系列。该系列以面向... 详细信息

鄂尔多斯盆地上古生界含气砂岩体主要属湖相三角洲平原分流河道沉积，具低孔低渗和高阻抗特征。针对这一地质目标，研制和总结出４类１５项方法技术，建立了鄂尔多斯盆地上古生界含气砂岩体综合预测目标处理解释技术系列。该系列以面向目标的大型综合数据库为基础，计算机技术为手段，地震地层岩性处理解释技术为核心，实现了地质、测井、地震等多学科的有机结合和同一地质目标下的处理解释一体化。在ＡＶＯ理论指导下，以信息论为基础，提出了一种新的测井约束多参数反演技术，可克服砂岩含气阻抗值降低而造成的岩性解释陷阱；利用不同岩性泊松比差异所形成的ＡＶＯ特征响应，可以有效区分地震高阻抗层的岩性；在实际钻探效益的驱动下，采用滚动式处理解释方法，应用各种新技术，对目标砂岩储集体实施逐步深化的特征描述。经钻探检验，该技术系列较准确地预测了厚１０ｍ左右砂岩储集体的空间展布形态，砂岩体预测符合率大于７０％，钻探成功率达８１．８％。

关键词：油气勘探地震数据处理预测盆地

蛋白激酶C-β抑制剂LY333531对早期糖尿病大鼠心脏抗氧化状态及NADPH氧化酶的影响

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武汉大学学报（医学版） 2011年第2期32卷 141-145,F0002页

作者：雷少青高霞刘慧敏徐波徐金金夏中元夏正远武汉大学医学院药理学教研室湖北武汉430071 武汉大学人民医院麻醉科湖北武汉430060 首都医科大学附属北京朝阳医院内分泌科北京100020 首都医科大学附属北京友谊医院内科北京100050

目的:探讨白蛋白激酶C-β抑制剂LY333531对早期糖尿病大鼠心脏抗氧化状态及NADPH氧化酶的影响。方法:24只SD雄性大鼠(240-260 g),随机分为正常对照组(NC)、糖尿病组(DM)及糖尿病LY333531治疗组(LY)。4周后试剂盒检测血浆及心肌组织中的1... 详细信息

目的:探讨白蛋白激酶C-β抑制剂LY333531对早期糖尿病大鼠心脏抗氧化状态及NADPH氧化酶的影响。方法:24只SD雄性大鼠(240-260 g),随机分为正常对照组(NC)、糖尿病组(DM)及糖尿病LY333531治疗组(LY)。4周后试剂盒检测血浆及心肌组织中的15-F2t-Isoprostane与总抗氧化浓度,HE染色光镜观察心肌形态学变化。Western bolt分析NADPH氧化酶亚基P22phox、P67phox及gP91phox蛋白水平改变。结果:与NC组比较,DM组心肌组织结构紊乱,血浆与心肌组织中15-F2t-Isoprostane及总抗氧化浓度水平明显增加,P22phox与gP91phox蛋白表达水平明显上调,P67phox膜转位明显增强(P<0.05)。与DM组比较,LY组心肌组织结构趋于正常,血浆与心肌组织中15-F2t-Isoprostane及血浆总抗氧化浓度水平明显减少,P22phox蛋白表达水平明显下调,P67phox膜转位明显减弱(P<0.05),但不能改变gP91phox表达水平。结论:LY333531通过抑制NADPH氧化酶表达的增加及活性的增强,减少糖尿病大鼠心肌组织的氧化损伤,从而发挥其心脏保护作用。

关键词： LY333531 糖尿病心肌病 NADPH氧化酶氧化应激

14例潜伏期新型冠状病毒肺炎患者手术室传染性及术后转归观察

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武汉大学学报（医学版） 2020年第5期41卷 702-705页

作者：苏娃婷雷少青贾一帆夏正远夏中元武汉大学人民医院麻醉科湖北武汉430060

目的:回顾性分析潜伏期新型冠状病毒肺炎(COVID-19)患者在手术室环境下的传染性及术后临床转归特征。方法:选取2020年1月份在武汉大学人民医院手术室诊治的处于潜伏期的14例COVID-19患者为研究对象,调查手术室医护人员与患者接触感染情... 详细信息

目的:回顾性分析潜伏期新型冠状病毒肺炎(COVID-19)患者在手术室环境下的传染性及术后临床转归特征。方法:选取2020年1月份在武汉大学人民医院手术室诊治的处于潜伏期的14例COVID-19患者为研究对象,调查手术室医护人员与患者接触感染情况,分析患者一般情况、临床症状与体征及术后转归情况。结果:手术室有42名医护人员(共47人次)与患者接触而被居家隔离或医学观察,最终有3名医护人员被确诊感染。14例潜伏期患者在术后3~7 d均被确诊感染(SARS-CoV-2核酸阳性);在症状体征方面,12例术后出现发热、10例感到疲劳乏力、9例出现干咳;在术后转归方面,1例患者死亡,13例(包括1例轻型、7例普通型、5例重型)患者转至定点单位继续隔离观察治疗。结论:潜伏期COVID-19患者在手术室环境下亦具有较高的传染性,应提高疫情期间医护人员的防护级别;麻醉手术应激会加重新冠肺炎患者病情,恶化患者术后转归,疫情爆发期间不宜进行择期手术。

关键词：新型冠状病毒肺炎临床特征潜伏期传染性术后转归

异丙酚对H_2O_2增强肿瘤坏死因子-α诱导血管内皮细胞凋亡效应的抑制作用

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武汉大学学报（医学版） 2006年第3期27卷 338-341页

作者：王芳夏中元罗涛欧阳静萍夏正远武汉大学人民医院麻醉科武汉430060 武汉大学医学院病理生理学系武汉430071

目的:探索H2O2对TNF-α诱导血管内皮细胞凋亡的作用及异丙酚(propofol)对细胞凋亡的保护作用和可能的机制。方法:将体外培养的ECV304细胞分为5组:①对照组(Control);②10μmol/L H2O2组(H);③40 nmol/L TNF-α组(T);④TNF-α+H2O2组(T+... 详细信息

目的:探索H2O2对TNF-α诱导血管内皮细胞凋亡的作用及异丙酚(propofol)对细胞凋亡的保护作用和可能的机制。方法:将体外培养的ECV304细胞分为5组:①对照组(Control);②10μmol/L H2O2组(H);③40 nmol/L TNF-α组(T);④TNF-α+H2O2组(T+H);⑤TNF-α+H2O2+propofol组(T+H+P)。观察:①流式细胞仪检测细胞凋亡率;②丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量。结果:10μmol/L的H2O2仅造成少量细胞凋亡,MDA含量变化不显著(P>0.05);与TNF-α共同作用后细胞凋亡率显著增加,且高于二者分别作用之和,细胞内MDA含量也明显增加,SOD和GSH-Px含量明显减少,与TNF-α组相比差异具有显著性(P<0.05);加入异丙酚预处理可使TNF-α和H2O2组凋亡率明显降低,同时伴随细胞内MDA含量降低,SOD和GSH-Px含量增加。结论:H2O2能显著增强TNF-α诱导细胞凋亡的效应,异丙酚通过降低细胞氧化损伤的机制,有效逆转H2O2增强TNF-α诱导细胞凋亡的效应。

关键词：异丙酚内皮细胞凋亡 H2O2 肿瘤坏死因子-α

糖尿病鼠早期心脏抗氧化能力及心功能改变与脂质过氧化反应的关系

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武汉大学学报（医学版） 2010年第1期31卷 16-18,99页

作者：徐波雷少青刘慧敏徐金金夏中元夏正远首都医科大学附属北京友谊医院内科北京100050 武汉大学人民医院麻醉科湖北武汉430060 武汉大学医学院药理学教研室湖北武汉430071

目的:建立大鼠1型糖尿病模型,探讨糖尿病早期心脏抗氧化能力及心功能改变与氧自由基所致脂质过氧化反应的关系。方法:Wistar鼠16只,体重(250±10)g,随机分为对照组(n=8)及糖尿病组(n=8)。糖尿病模型通过尾静脉注射链脲佐菌素(60 mg/kg)... 详细信息

目的:建立大鼠1型糖尿病模型,探讨糖尿病早期心脏抗氧化能力及心功能改变与氧自由基所致脂质过氧化反应的关系。方法:Wistar鼠16只,体重(250±10)g,随机分为对照组(n=8)及糖尿病组(n=8)。糖尿病模型通过尾静脉注射链脲佐菌素(60 mg/kg)制备。成功制备糖尿病模型后4周观察心脏功能、心肌总抗氧化能力及脂质过氧化产物15-F2t-Isoprostane变化及血清总抗氧化浓度。结果:与对照组相比,4周糖尿病鼠血清及心脏15-F2t-Isoprostane浓度无差异,但糖尿病鼠心脏组织在抗氧化剂t-butylhydroperoxide(t-BHP)刺激下,硫代巴比妥酸反应物质(TBARS)产物显著增强,反映心脏抗氧化能力降低,同时伴有心脏射血分数的显著下降(P<0.05)。结论:糖尿病鼠早期心脏抗氧化能力及心功能的减退早于脂质过氧化损伤的增加。

关键词：糖尿病鼠抗氧化能力心功能脂质过氧化物