检索结果-南通市图书馆

Mimotope peptide modified pompon mum-like magnetic microparticles for precise recognition,capture and biotransformation analysis of rituximab in biological fluids

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 1317-1328页

作者： Jiawen Yang Aixuan Zhou Minyi Li Qiaoxian He Jingwei Zhou Jacques Crommen Wentao Wang zhengjin jiang Qiqin Wang Institute of Pharmaceutical Analysis College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of ChinaJinan UniversityGuangzhou 510632China Laboratory for the Analysis of Medicines Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium SCIEX Shanghai 200050China

Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge challenge.In this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMPs,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first time.Benefit from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMPs,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported biomaterials.Leveraging the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum samples.We also established an LC-MS/MS method using the MMPs for tracking rituximab biotransformation in patient serum.Intriguingly,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful monitoring.Overall,these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.

关键词： Therapeutic monoclonal antibody Mimotope peptide Precise recognition Peptide-based biomaterials Biotransformation Patient serum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-throughput discovery of highly selective reversible hMAO-B inhibitors based on at-line nanofractionation

引用

Acta Pharmaceutica Sinica B 2024年第4期14卷 1772-1786页

作者： Yu Fan Jincai Wang Jingyi Jian Yalei Wen Jiahao Li Hao Tian Jacques Crommen Wei Bi Tingting Zhang zhengjin jiang Institute of Pharmaceutical Analysis/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of China/College of Pharmacy Jinan UniversityGuangzhou 510632China KU Leuven-University of Leuven Pharmaceutical AnalysisDepartment of Pharmaceutical and Pharmacological SciencesLeuven 3000Belgium Laboratory of Analytical Pharmaceutical Chemistry Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium Department of Neurology the First Affiliated Hospital of Jinan University/Clinical Neuroscience Institutethe First Affiliated Hospital of Jinan UniversityGuangzhou 510630China

Human monoamine oxidase B(hMAO-B)has emerged as a pivotal therapeutic target for Parkinson's disease.Due to adverse effects and shortage of commercial drugs,there is a need for novel,highly selective,and reversible hMAO-B inhibitors with good blood-brain barrier permeability.In this study,a high-throughput at-line nanofractionation screening platform was established with extracts from Chuanxiong Rhizoma,which resulted in the discovery of 75 active compounds,including phenolic acids,volatile oils,and phthalides,two of which were highly selective novel natural phthalide hMAO-B inhibitors that were potent,selective,reversible and had good blood‒brain permeability.Molecular docking and molecular dynamics simulations elucidated the inhibition mechanism.Sedanolide(IC_(50)=103 nmol/L;SI=645)and neocnidilide(IC_(50)=131 nmol/L;SI=207)demonstrated their excellent potential as hMAO-B inhibitors.They offset the limitations of deactivating enzymes associated with irreversible hMAO-B inhibitors such as rasagiline.In SH-SY5Y cell assays,sedanolide(EC_(50)=0.962μmol/L)and neocnidilide(EC_(50)=1.161μmol/L)exhibited significant neuroprotective effects,comparable to the positive drugs rasagiline(EC_(50)=0.896μmol/L)and safinamide(EC_(50)=1.079μmol/L).These findings underscore the potential of sedanolide as a novel natural hMAO-B inhibitor that warrants further development as a promising drug candidate.

关键词： Human monoamine oxidase B inhibitors High-throughput screening At-linenanofractionation Parkinson's disease Cellular neuroprotection Traditional Chinese medicines(TCMs)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography

引用

Journal of Pharmaceutical Analysis 2022年第2期12卷 332-338页

作者： Peijie Zhu Weijia Chen Qiqin Wang Huihui Wu Meng Ruan Hongwu Wang zhengjin jiang School of Food&Pharmaceutical Engineering Zhaoqing UniversityZhaoqingGuangdong526061China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China School of Pharmaceutical Engineering Guangdong Food and Drug Vocational CollegeGuangzhou510632China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China

In this research,a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane environment.Excellent physicochemical properties of this novel monolith that were achieved included column efficiency,stability,and permeability.Moreover,the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small molecules.In particular,it exhibited good potential as an alternative to the commercial immobilized artificial membrane(IAM)column(IAM.PC.DD2)for studying drug-membrane interactions.This study not only enriched the types of IAM stationary phases,but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates.

关键词： Phosphatidylethanolamine Biomimetic monolith IAM Drug-membrane interaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography

引用

Journal of Pharmaceutical Analysis 2022年第5期12卷 783-790页

作者： Qiqin Wang Lingjue Sun Huihui Wu Ning Deng Xianglong Zhao Jingwei Zhou Tingting Zhang Hai Han zhengjin jiang Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China Anhui Prevention and Treatment Center for Occupational Disease Anhui No.2 Provincial People's HospitalHefei230041China Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education Lab of BiochemistryCollege of ChemistryXiangtan UniversityXiangtanHunan411105China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China

Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction chromatography.In this study,a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-1-propanesulfonate(SBVI)with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic system.Notably,the duration for the preparation of this novel monolith was as little as 5 min,which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated copolymerization.Moreover,these monoliths showed good morphology,permeability,porosity(62.4%),mechanical strength(over 15 MPa),column efficiency(51,230 plates/m),and reproducibility(relative standard deviations for all analytes were lower than 4.6%).Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based monolith.In particular,the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein adsorption.These excellent features seem to favor its application in bioanalysis.Therefore,the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex samples.In this study,we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.

关键词： Zwitterionic monolith Sulfobetaine Photo-initiated copolymerization Hydrophilic interaction chromatography Complex sample

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Editorial of Special Column “Natural Product Screening”

引用

Acta Pharmaceutica Sinica B 2020年第10期10卷 1798-1799,1797页

作者： Ruin Moaddel zhengjin jiang National Institute on Aging National Institutes of HealthBaltimoreMD 21224USA College of Pharmacy Jinan UniversityGuangzhou 510632China

Nature has produced a rich diversity of structurally complex compounds or secondary metabolites with a wide variety of biological activities.Natural products have long been exploited for therapeutic purposes in traditional medicines around the world.Recently,it was reported almost half of the 1562 new approved drugs introduced between 1981 and 2014 are natural products or derived from natural products or biological^1.

关键词： drugs therapeutic Natural

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

引用

Journal of Pharmaceutical Analysis 2019年第4期9卷 254-258页

作者： Yang Huang Tiejie Wang Marianne Fillet Jacques Crommen zhengjin jiang Institute of Pharmaceutical Analysis College of Pharmacy Jinan University Guangzhou 510632 China Shenzhen Institute for Drug Control Shenzhen China Department of Pharmaceutics State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China Laboratory for the Analysis of Medicine Department of Pharmaceutical Sciences CIRM University of Liege CHU B36 B-4000 Liege Belgium

Tea is a widely consumed beverage and has many important physiological properties and potential health benefits. In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was fully validated and successfully applied to the determination of these amino acids in six different tea samples. Good linearity (r ≥ 0.993), precision (RSDs≤ 2.99%), accuracy (91.95%-107.09%) as well as good sample stability were observed. The limits of detection ranged from 1.42 to 14.69 ng/mL, while the limits of quantification were between 4.53 and 47.0 ng/mL. The results indicate that the contents of the 11 amino acids in the six different tea samples are greatly influenced by the degree of fermentation. The proposed SFC-MS method shows a great potential for further investigation of tea varieties.

关键词： Amino acids Teas Supercritical fluid chromatography with single quadrupole mass spectrometry (SFCMS)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11- dihydroquinidine-silica hybrid monolithic column

引用

Journal of Pharmaceutical Analysis 2020年第1期10卷 70-77页

作者： Dongsheng Xu Elena Sanchez-Lopez Qiqin Wang zhengjin jiang María Luisa Marina Departamento de Química Analítica Química Física e Ingeniería QuímicaUniversidad de AlcalaCtra.Madrid-Barcelona Km.33.60028871Alcala de Henares(Madrid)Spain Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China Instituto de Investigacion Química“Andres M.del Río”(IQAR) Universidad de AlcalaCtra.Madrid-Barcelona Km.33.60028871Alcala de Henares(Madrid)Spain

An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine(MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl(FMOC)derivatized amino acids by nano-liquid chromatography.The mobile phase was optimized including the apparent pH,content of ACN,and concentration of the buffer to obtain a satisfactory enantioresolution performance.27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested,and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of them.Analytical characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity,precision,accuracy,limits of detection(LOD)and quantitation(LOQ)showing good performance to be applied to the enantiomeric determination of these amino acids in dietary supplements.LOD and LOQ values were 9.3 and 31 mM for norvaline enantiomers and 7.5 and 25 mM for tryptophan enantiomers,respectively.The contents of D-norvaline and D-tryptophan were below their respective LODs in all the analyzed samples.Quantitation of L-tryptophan and L-norvaline showed good agreement with the labeled contents except for one sample which did not show presence of L-norvaline,contrary to the label indication.

关键词： 9-Fluorenylmethoxycarbonyl Amino acids Chiral separation Nano-LC Quinidine-silica hybrid monolithic column

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rapid identification and isolation of neuraminidase inhibitors from mockstrawberry(Duchesnea indica Andr.) based on ligand fishing combined with HR-ESI-Q-TOF-MS

引用

Acta Pharmaceutica Sinica B 2020年第10期10卷 1846-1855页

作者： Sifan Luo Linbo Guo Caimin Sheng Yumei Zhao Ling Chen Chufang Li zhengjin jiang Haiyan Tian Institute of Traditional Chinese Medicine and Natural Products College of PharmacyJinan UniversityGuangzhou 510632China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou 510632China The State Key Laboratory of Respiratory Disease and the 1st Affiliated Hospital of Guangzhou Medical University Guangzhou 510663China International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education(MOE) College of PharmacyJinan UniversityGuangzhou 510632China

Neuraminidase inhibitors(NAIs)are the mainstay antiviral drugs against influenza infection.In this study,a ligand fishing protocol was developed to screen NAIs using neuraminidase immobilized magnetic beads(NA-MB).After verifying the feasibility of NA-MB with an artificial mixture including NA inhibitors and non-inhibitors,the developed ligand fishing protocol was applied to screen NAIs from the crude extracts of Duchesnea indica Andr.Twenty-four NA binding compounds were identified from the normal butanol(n-BuOH)extract of D.indica as potential NAIs by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS)assisted with Compound Structure Identification(CSI):FingerID,including 12 ellagitannins,4 brevifolin derivatives,3 ellagic acid derivatives,and 4 flavonoids.Among them,9 compounds were isolated and tested for in vitro NA inhibitory activities against NA from Clostridium perfringens,and from oseltamivir sensitive and resistant influenza A virus strains.The results indicate that compound B23 has the NA inhibitory activities in both the oseltamivir sensitive and resistant viral NA,with half maximal inhibitory concentration(IC50)values of 197.9 and 125.4μmol/L,respectively.Moreover,B23 can obviously reduce the replication of oseltamivir sensitive and resistant viruses in Madin-Darby canine kidney(MDCK)cells at the concentrations of 40 and 200μmol/L.

关键词： Neuraminidase inhibitors Duchesnea indica Andr. Ligand fishing Magnetic beads HPLCe Q-TOF-MS Influenza A Virus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Research progress on the divergence and genetic basis of agronomic traits in xian and geng rice

引用

The Crop Journal 2022年第4期10卷 924-931页

作者： Linlin jiang Lian Wu Yun Wang Quan Xu zhengjin Xu Wenfu Chen Rice Research Institute of Shenyang Agricultural University Shenyang 110866LiaoningChina Agronomy College of Shenyang Agricultural University Shenyang 110866LiaoningChina

The Asian cultivated rice Oryza sativa can be classified into two major subspecies:japonica/geng and indica/xian.There are large physiological and phenotypic differences between the two subspecies,with each having its advantages and disadvantages.Understanding the differences between xian and geng could provide a foundation for cultivar improvement based on hybridization between subspecies in order to synthesize favorable traits.We review the origin and domestication of xian and geng rice,compare their differences in terms of physiological and phenotypical traits,and describe the molecular mechanism differences between the subspecies.Based on this knowledge,we propose an ideal plant architecture of geng rice varieties for northern regions.

关键词： Oryza sativa Differentiation between subspecies Genetic basis Plant architecture of geng rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of the conductive behavior of a simplified sediment system and its computational simulation

引用

International Journal of Sediment Research 2020年第3期35卷 249-255页

作者： zhengjin Weng Zhiwei Zhao Yong Fang Helong jiang Wei Lei School of Electronic Science and Engineering Southeast University State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnotogyChinese Academy of Sciences

Because of the important contributions of electrochemical redox reactions to biochemical cycles and their potential application for the in-situ remediation of contaminated sediment,the mechanisms of long-distance electron transport coupling spatially separated redox half reactions in sediment have drawn much attention.To explore a preliminary mechanism of long-distance electron transport in sediment,in the current study,two simplified composite systems are constructed consisting of spherical ferroferric oxide(FeO) nanoparticles and rod-like carbon nanotubes(CNTs) as conductive fillers and silica(SiO) particles as the matrix.Two different constructed composite systems(e.g.,SiO/Fe3 O4 and SiO/Fe3 O4/CNTs) were used to model a three-dimensional sediment framework instead of sediment with quite co mplex components.The effects of the loading of conductive fillers(e.g.,FeO,CNTs) and the particle size of SiOmatrix on the conductive behavior of the composite system were investigated.The results showed that both of the electrical properties of SiO/Fe3 O4 and SiO/Fe3 O4/CNTs composite systems typically exhibited a non-linear conductive behavior that the electrical conductivity increased with the increasing of filler loading and showed an abrupt increase at critical filler loading.The conductivity of the SiO/FeOand SiO/Fe3 O4/CNTs composite systems with micro-sized SiOas the matrix was higher than that of the composite systems with nano-sized SiOas the matrix.Compared with the SiO/FeOcomposite system,the electrical conductivity of the SiO/Fe3 O4/CNTs composite system was enhanced by several orders of magnitude and only a small loading of CNTs could make the conductivity of the SiO/FeO/CNTs composite system reach a higher level.The electrical conductivity predicted by the electrical conductivity model of a two-phase composite system showed a similar trend as the experimental results and the two-dimensional(2 D) percolation-based model filled with rods gave a good estimation of percolation probability.

关键词： Sediment Ferroferric oxide Carbon nanotubes Conductive behavior Simulation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：