Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography
作者机构：Institute of Pharmaceutical AnalysisCollege of PharmacyJinan UniversityGuangzhou510632China Anhui Prevention and Treatment Center for Occupational DiseaseAnhui No.2 Provincial People's HospitalHefei230041China Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of EducationLab of BiochemistryCollege of ChemistryXiangtan UniversityXiangtanHunan411105China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug ResearchJinan UniversityGuangzhou510632China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2022年第12卷第5期
页 面：783-790页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 10[医学]
基 金：supported by the National Natural Science Foundation of China(Grant Nos.:82173773 and 82073806) the Natural Science Foundation of Guangdong Province,China(Grant Nos.:2020A1515010569 and 2021A0505030039) Science and Technology Program of Guangzhou,China(Grant No.:202102020729)
主 题：Zwitterionic monolith Sulfobetaine Photo-initiated copolymerization Hydrophilic interaction chromatography Complex sample
摘 要：Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction *** this study,a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-1-propanesulfonate(SBVI)with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic ***,the duration for the preparation of this novel monolith was as little as 5 min,which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated ***,these monoliths showed good morphology,permeability,porosity(62.4%),mechanical strength(over 15 MPa),column efficiency(51,230 plates/m),and reproducibility(relative standard deviations for all analytes were lower than 4.6%).Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based *** particular,the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein *** excellent features seem to favor its application in ***,the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex *** this study,we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.
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