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InDel and SNP Markers and Their Applications in Map-based Cloning of Rice Genes

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Rice science 2008年第4期15卷 251-258页

作者： Cun-hong PAN[a][b] Ai-hong LI[a] zheng-yuan dai[a] Hong-xi ZHANG[a] Guang-qing LIU[a] Zi-bin WANG[b] Yu-yin MA[b] Yue-jun YIN[b] Ya-fang ZHANG[b] Shi-min ZUO[b] Zong-xiang CHEN[b] Xue-biao PAN[b] [a] Lixiahe Agricultural Research Institute of Jiangsu Province Yangzhou 225007 China [b] Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 China

High-density markers are necessary for map-based cloning of rice genes, but the currently available markers are not satisfactory enough. InDel (insertion-deletion length polymorphism) and SNP (single nucleotide polymorphism) are the new generation of molecular markers and can basically meet the need of fine mapping. InDel and SNP markers can be developed through bioinformatics. These markers are valuable markers with the characters of low cost, high specificity and stability. This article introduced the methods for designing InDel and SNP markers, taking the mapping of a rice rolled leaf gene as an example. In addition, some key factors in improving the design efficiency were also discussed.

关键词： rice molecular marker insertion-deletion length polymorphism single nucleotide polymorphism bioinformatics gene mapping rolled leaf gene

Multiphysics simulation and optimization of the in-situ beneficiation process of ilmenite minerals from lunar regolith

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Science China Earth Sciences 2025年第1期68卷 195-207页

作者： Peng ZHANG Xin LIU Guanghui LIU Wei dai Hanzhe YANG Haibo zheng Zhi WANG Ran NIU Yifan BAI Yang ZHANG Chengbao LIU Ge YANG Junwei YANG Guang ZHANG Technology and Engineering Center for Space Utilization Chinese Academy of Sciences Beijing Hegong Simulation Co. Ltd

The exploration and utilization of lunar resources have become the major objectives of future lunar exploration missions. The classified collection and processing of lunar minerals is the basic prerequisite for the in-situ exploration and utilization of lunar resources. In this study, we propose a scheme for in-situ separation of magnetic minerals from lunar soils using the dry magnetic separation method. The scheme can realize the purpose of separating magnetic minerals from lunar regolith by altering the motion trajectory of lunar regolith particles with different magnetic properties. Detailed modeling of the dry magnetic separation device is conducted, and the three-dimensional distribution of magnetic parameters is calculated and analyzed. Based on the characterization and analysis of the Chang E-5's samples, the morphologies and physical parameters of lunar regolith particles are modeled and simulated. A discrete element simulation of the motion process of lunar regolith particles under multi-physical field conditions is performed, and the influences of key parameters on the beneficiation performance of lunar magnetic minerals are investigated. Our results indicate that optimal separation and beneficiation of lunar regolith particles can be achieved under specific conditions of magnetic field strength and drum speed. Additionally, we analyze the effects of lunar weak gravity conditions and the charged nature of lunar particles on the beneficiation process, showing that these factors can obviously enhance the beneficiation process of lunar ilmenites. This study demonstrates the feasibility of using the dry magnetic separation method for the beneficiation of magnetic minerals from lunar regolith, providing a theoretical basis for the scientific tasks of collecting, processing, and utilizing lunar mineral resources.

关键词： Lunar regolith In-situ resource utilization Magnetic separation Classified sampling

The compound(E)-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one alleviates neuroinflammation and cognitive impairment in a mouse model of Alzheimer's disease

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Neural Regeneration Research 2025年第11期20卷 3330-3344页

作者： Xueyan Liu Wei Wu Xuejuan Li Chengyan Wang Ke Chai Fanru yuan Huijuan zheng Yuxing Yao Chenlu Li Zu-Cheng Ye daijun Zha Department of Medicinal Chemistry School of PharmacyFujian Medical UniversityFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Brain Aging and Neurodegenerative Diseases School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Institute of Laboratory Animal Center Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurosurgery Neurosurgery Research InstituteThe First Affiliated HospitalFujian Medical UniversityFuzhouFujian ProvinceChina Department of Hyperbaric Oxygen National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhouFujian ProvinceChina

Previous studies have shown that the compound(E)-2-(3,4-dihydroxystyryl)-3-hydroxy-4H-pyran-4-one(D30),a pyromeconic acid derivative,possesses antioxidant and anti-inflammatory properties,inhibits amyloid-β aggregation,and alleviates scopolamine-induced cognitive impairment,similar to the phase Ⅲ clinical drug *** this study,we established a mouse model of Alzheimer's disease via intracerebroventricular injection of fibrillar amyloid-β to investigate the effect of D30 on fibrillar amyloid-β-induced *** results showed that D30 alleviated fibrillar amyloid-β-induced cognitive impairment,promoted fibrillar amyloid-β clearance from the hippocampus and cortex,suppressed oxidative stress,and inhibited activation of microglia and astrocytes.D30 also reversed the fibrillar amyloid-β-induced loss of dendritic spines and synaptic protein ***,we demonstrated that exogenous fibrillar amyloid-βintroduced by intracerebroventricular injection greatly increased galectin-3 expression levels in the brain,and this increase was blocked by *** the role of D30 in clearing amyloid-β,inhibiting neuroinflammation,protecting synapses,and improving cognition,this study highlights the potential of galectin-3 as a promising treatment target for patients with Alzheimer's disease.

关键词： Alzheimer's disease amyloid-β astrocyte cognitive impairment D30 dendritic spines galectin-3 microglia neuroinflammation neuron

Selective G6PDH inactivation for Helicobacter pylori eradication with transformed polysulfide

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Science China Life Sciences 2025年

作者： Xiaonan Wang Ning Zhou Xuejiao J.Gao Zijing Zhu Minmin Sun Qian Wang Haolin Cao Xuetong Wu Caiyu Zhou Qingkang zheng Ye yuan yuan Liu Lei Chen Jing Jiang Pengcheng Bu Lizeng Gao CAS Engineering Laboratory for Nanozyme Key Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences School of Life Sciences University of Chinese Academy of Sciences College of Chemistry and Chemical Engineering Jiangxi Normal University Key Laboratory of Epigenetic Regulation and Intervention Institute of Biophysics Chinese Academy of Sciences Nanozyme Laboratory in Zhongyuan School of Basic Medical Sciences Zhengzhou University Nanozyme Laboratory in Zhongyuan Henan Academy of Innovations in Medical Science

Alternative treatment for the highly prevalent Helicobacter pylori infection is imperative due to rising antibiotic resistance. We unexpectedly discovered that the anti-H. pylori component in garlic is hydrogen polysulfide（H2S_n, n≥2）, not organic polysulfides. Studies on the mechanism of action（MoA） show that H2S_nspecifically inactivates H. pylori glucose-6-phosphate dehydrogenase（G6PDH） by interfering with electron transfer from glucose-6-phosphate（G6P） to nicotinamide adenine dinucleotide phosphate（NADP+）. However, low H2S_nyield makes garlic derivatives hard to be a reliable donor of H2S_nto treat H. pylori infection. To address this challenge, we established a polysulfide transformation process from garlic organosulfur compounds into Fe3S4that generates H2S_nwith a 25–58 times increase in *** chitosan encapsulation, we designed a gastric-adaptive H2S_nmicroreactor（GAPSR） that eradicates H. pylori with 250 times higher efficiency under gastric conditions. A single GAPSR achieves more rapid H. pylori eradication than combined antibiotics therapy without disturbing the gut microbiota. These findings indicate a distinct MoA transformation mediated by polysulfide as an alternative candidate to treat H. pylori infection.

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A stretchable, ionic conductive, and adhesive patch electrode with ultra-low on-skin impedance for electrophysiological signal recording

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Science China(Information Sciences) 2025年

作者： Yang LI Yuzhe GU Sheng QIAN Yuncong PANG Aoxi YU Shuwen zheng Wenjie XIA yuan LIAO Baoguang LIU Shujuan LIU Qiang ZHAO College of Electronic and Optical Engineering & College of Flexible Electronics (Future Technology) Nanjing University of Posts & Telecommunications (NJUPT) State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications (NJUPT)

Flexible electrodes have significant potential in fields such as healthcare and neuroscience, as they enable comprehensive recording and analysis of human electrical signals, providing deeper insights into physiological conditions and the state of internal organs and tissues [1, 2]. However, commercial Ag/AgCl wet electrodes face issues such as dehydration,poor comfort, and insufficient mechanical strength, while other types of electrodes, like dry and semi-dry electrodes,also have their own limitations, including the higher contact impedance. In contrast, ionically conductive electrodes offer potential advantages such as transparency, stability, and low impedance, making them a promising alternative to existing electrically conductive electrodes [3]. Acrylic acid (AA)and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) are commonly used in the synthesis of ionic gels, as they ionize in water to produce a large number of ions, helping to maintain moisture balance and reduce impedance caused by dehydration [4]. However, the presence of freely moving ions in the gel network can interfere with conductivity, highlighting the need to introduce new conductive networks to enhance performance [5].

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紫外线B辐射增强和酸氮沉降复合作用对杉木凋落物分解的影响

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东北林业大学学报 2025年第1期53卷 57-63页

作者：郑立津戴渊陈水兰刘爱琴福建农林大学福州350002

为了探究不同环境因子复合作用对杉木(Cunninghamia lanceolata)凋落物分解机制的影响,以杉木人工林凋落物为研究对象,采用野外环境控制试验方法,通过设置不同紫外线B辐射增强、酸沉降和氮沉降试验处理,进行紫外线B辐射增强与酸氮沉降... 详细信息

为了探究不同环境因子复合作用对杉木(Cunninghamia lanceolata)凋落物分解机制的影响,以杉木人工林凋落物为研究对象,采用野外环境控制试验方法,通过设置不同紫外线B辐射增强、酸沉降和氮沉降试验处理,进行紫外线B辐射增强与酸氮沉降复合作用对杉木凋落物分解过程处理1年的定位研究。结果表明:不同试验处理后,杉木凋落物的分解速率从大到小依次为氮沉降处理(0.69)、紫外线B辐射增强与氮沉降复合处理(0.64)、紫外线B辐射增强与酸氮沉降复合处理(0.59)、酸沉降与氮沉降复合处理(0.58)、对照(0.56)、紫外线B辐射增强处理(0.55)、酸沉降处理(0.54)、紫外线B辐射增强与酸沉降复合处理(0.52)。氮沉降处理、紫外线B辐射增强与氮沉降复合处理会促进凋落物分解,缩短凋落物分解的周期;酸沉降处理会减缓杉木凋落物分解的速率。紫外线B辐射增强、紫外线B辐射增强和酸沉降复合处理、酸沉降和氮沉降复合处理、酸沉降氮沉降和紫外线B复合处理,对杉木凋落物分解的速率没有明显的影响。

关键词：氮沉降酸沉降紫外线B辐射增强杉木凋落物

铜基催化剂在乙炔氢氯化反应中的工业应用评估

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现代化工 2025年第1期45卷 72-76页

作者：代元元杨京林牛强内蒙古鄂尔多斯电力冶金集团股份有限公司内蒙古鄂尔多斯016064

概述了近年来国内外铜催化剂在乙炔氢氯化反应中的研究进展,综述了铜催化剂的几种改性手段,探讨了催化剂的工业应用情况,分析了铜催化剂工业应用的安全风险问题。研究表明,经过适当改性,铜催化剂具备工业应用潜力,且在实际工业生产中通... 详细信息

概述了近年来国内外铜催化剂在乙炔氢氯化反应中的研究进展,综述了铜催化剂的几种改性手段,探讨了催化剂的工业应用情况,分析了铜催化剂工业应用的安全风险问题。研究表明,经过适当改性,铜催化剂具备工业应用潜力,且在实际工业生产中通过严格控制工艺指标可避免铜工业应用的安全风险问题。

关键词：乙炔氢氯化铜催化剂工业化应用安全性

胞外漆酶同工酶谱及其在食用菌育种中的应用——Ⅰ.黑木耳及光帽鳞伞不同菌株的鉴别

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真菌学报 1987年第3期 178-183页

作者：方自若郑美媛杨岱筠黑龙江省科学院应用微生物研究所黑龙江省科学院应用微生物研究所哈尔滨

利用聚丙烯酰胺凝胶等电聚焦圆盘电泳,分析比较了黑木耳(Auricularia auricula)16个菌株和光帽鳞伞(Pholiota nameko)9个菌株的胞外漆酶同工酶酶谱。经多批次重复,得到了所有菌株的恒定酶谱。依照这些酶谱及各酶带等电点(pI)值的不同,... 详细信息

利用聚丙烯酰胺凝胶等电聚焦圆盘电泳,分析比较了黑木耳(Auricularia auricula)16个菌株和光帽鳞伞(Pholiota nameko)9个菌株的胞外漆酶同工酶酶谱。经多批次重复,得到了所有菌株的恒定酶谱。依照这些酶谱及各酶带等电点(pI)值的不同,确立了它们的漆酶标志位点相似系谱。16株黑木耳被区分成4种类型9个组。9株光帽鳞伞被分成两种类型3个组,但各组内之菌株间仍有异同。试验结果表明,把漆酶同工酶谱应用于菌株鉴别将是一种有意义的方法。

关键词：漆酶同工酶等电聚焦黑木耳光帽鳞伞等电点值(pI) 基因型

AN ADAPTIVE CONTROL STRATEGY FOR PROPER MESH DISTRI-BUTION IN LARGE EDDY SIMULATION

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Journal of Hydrodynamics 2010年第6期22卷 865-870页

作者： ZHANG Bin WANG Tong GU Chuan-gang dai zheng-yuan Key Laboratory for Power Machinery and Engineering of Ministry of Education Shanghai Jiao Tong University Shanghai 200240 China Trane＇s Asia-Pacific Research Center Shanghai 200001 China

The Filtering Grid Scale （FGS） of sub-grid scale models does not match with the theoretical Proper FGS （PFGS） because of the improper mesh. Therefore, proper Large Eddy Simulation （LES） Mesh is very decisive for better results and more economical cost. In this work, the purpose is to provide an adaptive control strategy for proper LES mesh with turbulence theory and CFD methods. A new expression of PFGS is proposed on the basis of -5/3 law of inertial sub-range and the proper mesh of LES can be built directly from the adjustment of RANS mesh. A benchmark of the backward facing step flow at Re = 5147 is provided for application and verification. There are three kinds of mesh sizes, including the RANS mesh, LAM （LES of adaptive-control mesh）, LFM （LES of fine mesh）, employed here. The grid number of LAM is smaller than those of LFM evidently, and the results of LAM are in a good agreement with those of DNS and experiments. It is revealed that the results of LAM are very close to those of LFM. The conclusions provide positive evidences for the novel strategy.

关键词： Large Eddy Simulation (LES) Proper Filtering Grid Scale (PFGS) turbulence -5/3 law adaptive control