检索结果-南通市图书馆

Growth enhancement effect of BzATP on primary cultured astrocytes from rat brain

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2006年第3期22卷 151-158页

作者： Hua-zheng LIANG ying liu Zhu-Rong YE Department of Pathology Shanghai Medical College Fudan University Shanghai 200032 China

Objective To explore whether BzATP could promote the growth of primary cultured astrocytes （AS） of rat and its possible mechanism, and whether TGF-β1 was involved in the event. Methods The primary cultured AS were derived from new born Sprague-Dawley rats. Glial fibrillary acidic protein （GFAP） immunofluorescent stain was used to check the purity of cultured AS. Morphometry was used to detect the changes of AS. The proliferation index of AS was detected by BrdU incorporation assay. Western blot was used to detect the changes of GFAP under different conditions. Changes of TGF-β1 gene transcription were detected by RT-PCR. ELISA was utilized to detect the variation of TGF-β1 protein in the supernate. Results The purity of primary cultured AS reached to 99%. BzATP promoted the hypertrophy of AS including the elongation of AS processes and the enlargement of cell bodies, BzATP also promoted the expression of GFAP in existence of Ca^2＋, but had no effect on cell proliferation. BzATP increased the transcription of TGF-β1 mRNA and the release of TGF-β1 protein in existence of Ca^2＋. TGF-β1 neutralizing antibody partially inhibited the expression of GFAP induced by BzATP, but had no effect on AS proliferation and cell morphology. Conclusion BzATP enhanced the hypertrophy of primary cultured AS, increased the expression of GFAP partially through TGF-β1. Mechanisms of the enhancement of AS growth induced by BzATP other than TGF-β1 pathway remains to be elucidated.

关键词： astrocyte BzATP Ca^2+ TGF-β1

Inhibition of tumor necrosis factor-alpha by sodium ferulate in protecting neurons from beta-amyloid induced damage

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2006年第8期1卷 693-696页

作者： Suyan Yao Deyu zheng Zhuo liu ying Jin Department of Pathophysiology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Anatomy Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Pharmacology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Pharmacology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina

BACKGROUND: Sodium ferulate (SF) has an effect of anti-inflammation; however, whether it can inhibit beta-amyloid (Aβ) induced damage or not should be further studied. OBJECTIVE: To investigate the effects of SF on neurotoxicity mediated by Aβ-induced macrophage activation via inhibiting tumor necrosis factor-α (TNF-α) in vitro. DESIGN: A contrast experiment based on cells. SETTING: Departments of Pathophysiology, Pharmacology and Anatomy, Liaoning Medical College. MATERIALS: A total of 36 Kunming mice aged 8-10 weeks and some SD rats aged 2-3 days of both genders were selected in this study. Main reagents were detailed as follows: Aβ peptide (Sigma Company); SF (purity > 99%, Suzhou Changtong Chemical Co., Ltd.); lactate dehydrogenase (LDH) assay kit (Bangding Biological Engineering Co., Beijing, China); microtubule-associated protein 2 (MAP-2) monoclonal antibodies and TNF-α monoclonal antibodies (Boster Biological Engineering Co., Wuhan, China). METHODS: The experiment was carried out in Laboratories of Pharmacology and Anatomy, Liaoning Medical College from May to December 2004. Cerebellum was obtained from rats under sterile condition to culture neurons and macrophages taken from mice abdominal cavity. Later, two parallel experiments were performed as follows: ① Macrophages culture groups: In normal control group, macrophages were cultured in DMEM after being seeded. In Aβ group, neurotoxic form of Aβ was added into DMEM media with final concentration of 10 μmol/L after macrophages were seeded for 24 hours. In Aβ+SF group, ten minutes after Aβ treatment, for 10, 100, 500 μmol/L and 1 mmol/L of SF were added to the media of the macrophages culture. ② Macrophages-neurons co-cultured groups: Control macrophages-neurons were co-cultured. Aβ group: Neurotoxic form of Aβ was added into the media with concentration of 10 μmol/L after macrophages were seeded in the neurons cultured wells for 24 hours. Aβ+SF group: Ten minutes after Aβ treatment, 10, 100, 500 μmol/L and

关键词： TNF LDH

Development of Fixture Layout Optimization for Thin-Walled Parts:A Review

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2024年第1期37卷 15-39页

作者： Changhui liu Jing Wang Binghai Zhou Jianbo Yu ying zheng Jianfeng liu School of Mechanical Engineering Tongji UniversityShanghai 201804China Shanghai Jiaotong University Shanghai 200240China Shanghai Waigaoqiao Shipbuilding Co. Ltd.Shanghai 200137China

An increasing number of researchers have researched fixture layout optimization for thin-walled part assembly during the past ***,few papers systematically review these *** analyzing existing literature,this paper summarizes the process of fixture layout optimization and the methods *** process of optimization is made up of optimization objective setting,assembly variation/deformation modeling,and fixture layout *** paper makes a review of the fixture layout for thin-walled parts according to these three ***,two different kinds of optimization objectives are *** usually consider in-plane variations or out-of-plane deformations when designing ***,modeling methods for assembly variation and deformation are divided into two categories:Mechanism-based and data-based *** common methods are discussed *** that,optimization algorithms are reviewed *** are two kinds of optimization algorithms:Traditional nonlinear programming and heuristic ***,discussions on the current situation are *** research direction of fixture layout optimization in the future is discussed from three aspects:Objective setting,improving modeling accuracy and optimization ***,a new research point for fixture layout optimization is *** paper systematically reviews the research on fixture layout optimization for thin-walled parts,and provides a reference for future research in this field.

关键词： Thin-walled parts Assembly quality Fixture layout optimization Modeling methods Optimization algorithms

Optimization of Fixture Number in Large Thin-Walled Parts Assembly Based on IPSO

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2024年第2期37卷 213-227页

作者： Changhui liu Jing Wang ying zheng Ke Jin Jianbo Yu Jianfeng liu School of Mechanical Engineering Tongji UniversityShanghai 201804China Shanghai Jiaotong University Shanghai 200240China Shanghai Waigaoqiao Shipbuilding Co. Ltd.Shanghai 200137China

There are lots of researches on fixture layout optimization for large thin-walled *** researches focus on the positioning problem,i.e.,optimizing the positions of a constant number of ***,how to determine the number of fixtures is *** most cases,the number of fixtures located on large thin-walled parts is determined based on engineering experience,which leads to huge fixture number and extra ***,this paper constructs an optimization model to minimize the number of *** constraints are set in the optimization model to ensure that the part deformation is within the surface profile *** addition,the assembly gap between two parts is also *** conduct the optimization,this paper develops an improved particle swarm optimization(IPSO)algorithm by integrating the shrinkage factor and adaptive inertia *** the algorithm,particles are encoded according to the fixture *** dimension of the particle is assigned to a sub-region by constraining the optional position range of each fixture to improve the optimization ***,a case study on ship curved panel assembly is provided to prove that our method can optimize the number of fixtures while meeting the assembly quality *** research proposes a method to optimize the number of fixtures,which can reduce the number of fixtures and achieve deformation control at the same time.

关键词： Assembly quality Large thin-walled parts Fixture layout PSO FEM

Self-assembled core-shell polydopamine@MXene with synergistic solar absorption capability for highly efficient solar-to-vapor generation

在线全文

学校读者我要写书评

暂无评论

Nano Research 2020年第1期13卷 255-264页

作者： Xing Zhao Xiang-Jun Zha Li-Sheng Tang Jun-Hong Pu Kai Ke Rui-ying Bao zheng-ying liu Ming-Bo Yang Wei Yang College of Polymer Science and Engineering Sichuan UniversityState Key Laboratory of Polymer Materials EngineeringChengdu610065China

As a renewable and environment-friendly technology for seawater desalination and wastewater purification,solar energy triggered steam generation is attractive to address the long-standing global water scarcity ***,practical utilization of solar energy for steam generation is severely restricted by the complex synthesis,low energy conversion efficiency,insufficient solar spectrum absorption and water extraction capability of state-of-the-art ***,for the first time,we report a facile strategy to realize hydrogen bond induced self-assembly of a polydopamine(PDA)@MXene microsphere photothermal layer for synergistically achieving wide-spectrum and highly efficient solar absorption capability(≈96%in a wide solar spectrum range of 250–1,500 nm wavelength).Moreover,such a system renders fast water transport and vapor escaping due to the intrinsically hydrophilic nature of both MXene and PDA,as well as the interspacing between core-shell *** solar-to-vapor conversion efficiencies under the solar illumination of 1 sun and 4 sun are as high as 85.2%and 93.6%,***,the PDA@MXene photothermal layer renders the system durable mechanical properties,allowing producing clean water from seawater with the salt rejection rate beyond 99%.Furthermore,stable light absorption performance can be achieved and well maintained due to the formation of ternary TiO2/C/MXene complex caused by oxidative degradation of ***,this work proposes an attractive MXene-assisted strategy for fabricating high performance photothermal composites for advanced solar-driven seawater desalination applications.

关键词： self-assembly polydopamine(PDA)@MXene microsphere synergistic light absorption solar-to-vapor generation light absorption stability

Synthesis of Inorganic Silica Grafted Three-arm PLLA and Their Behaviors for PLA Matrix

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2019年第3期37卷 216-226页

作者： Da-Wei Shi Xiang-Ling Lai Yuan-Ping Jiang Cong Yan zheng-ying liu Wei Yang Ming-Bo Yang State Key Laboratory of Polymer Materials Engineering College of Polymer Science and Engineering Sichuan University

The low melt strength and poor crystallization behavior severely limit the processing and application of poly(lactic acid)(PLA) as biodegradable film materials. In this work, three-arm poly(L-lactic acid)(3A-PLLA) grafted silica nanoparticles with two kinds of topology structures were introduced into PLA matrix to improve the biodegradation performance. Different methods were used to characterize the structure of the grafted 3A-PLLA chains, the grafting density, and the thermal decomposition behavior of the nanoparticles. By varying the mass ratios of raw materials and altering the order of dropping solution in the reaction, high grafting densitytangled 3A-PLLA grafted SiO_2 was synthesized as "3A-PLLA grafting to SiO_2"(GTS), while low grafting density-stretched 3A-PLLA grafted SiO_2 was obtained as "SiO_2 grafting to 3A-PLLA"(GTA). Topology of nanoparticles as well as the filler-matrix interaction is critically important to structure bio-nanocomposites with desirable properties. Thus, the GTS and GTA nanoparticles were introduced into PLA matrix to assess the effect. The SEM images showed the uniform dispersion of the modified nanoparticles, while the shear rheology results revealed that GTA nanoparticles made a more significant contribution on the melt-strengthening and relaxation time-extension of PLA. Moreover, it is suggested that GTA nanoparticles were more effective to act as a nucleating agent for PLA, which was proved by differential scanning calorimetry(DSC) and polarized optical microscopy(POM) researches. All of the improvements mentioned above of GTA nanocomposites can be ascribed to stronger entanglements between 3A-PLLA stretched by nano-SiO_2 and PLA matrix.

关键词： Poly(lactic acid) Nanocomposites Surface grafting Topology Crystallization

New species of spongillafly genus Sisyra Burmeister,1839(Neuroptera:Sisyridae)from China and Laos

在线全文

学校读者我要写书评

暂无评论

Zoological Systematics 2024年第2期49卷 129-142页

作者： ying Yang Yuchen zheng Xingyue liu Department of Entomology China Agricultural UniversityBeijing 100193China

Three new spongillafly species of Sisyra Burmeister,1839 from southern China and Laos are described,namely *** ***.,*** ***.,and *** ***,*** *** *** *** remarkable forewing marking pattern,which has never been reported in ***,the male of the rare species Sisyra yunana Yang,1986 is described for the first *** these Sisyra species have a highly specialized male gonocoxites 9.

关键词： Aquatic insects Neuropterida taxonomy

Hierarchical Crystalline Structures Induced by Temperature Profile in HDPE Bars during Melt Penetration Process

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2017年第1期35卷 108-122页

作者： chun-xia zhu rui-yan zhang yan-hao huang wei yang zheng-ying liu jian-ming feng 杨鸣波 College of Polymer Science and Engineering State Key Laboratory of Polymer Materials Engineering Sichuan University Chengdu 610065 China

The hierarchical crystalline morphologies and orientation structures across the thickness direction in high-density polyethylene （HDPE） molded bars were investigated via a novel melt-penetrating processing method named multi-melt multi-injection molding （M3IM）. The samples with various mold temperatures （20, 40 and 60 ℃） were prepared, and the effects of the external temperature profile on the evolution of crystalline microstructures were studied. With scanning electron microscopy （SEM）, the transition of crystalline morphology from ring-banded structure to oriented lamellae was observed with decreasing mold temperature, and the oriented lamellae were formed at the sub-skin layer of the samples at the lowest mold temperature, which was further testified by differential scanning calorimetry （DSC）. With the decline of mold temperature, the degree of orientation, obtained from two-dimensional small angle X-ray scattering （2D-SAXS）, was increased and long periods rose a little. Thus, decreasing mold temperature was beneficial to the formation of orientation structures because the relaxation of chains was weakened.

关键词： Hierarchical structures Orientation Multi-melt multi-injection molding Mold temperature

Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots

在线全文

学校读者我要写书评

暂无评论

Horticultural Plant Journal 2024年第2期10卷 450-460页

作者： Huiyu Wang Yushan zheng Qian Zhou ying Li Tongkun liu Xilin Hou State Key Laboratory of Crop Genetics&Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNanjingJiangsu 210095China Nanjing Suman Plasma Engineering Research Institute Nanjing Agricultural UniversityNanjingJiangsu 210095China

Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu *** one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.

关键词： Agrobacterium rhizogenes Non-heading Chinese cabbage Transgenic roots Composite plant Transformation efficiency