Inhibition of tumor necrosis factor-alpha by sodium ferulate in protecting neurons from beta-amyloid induced damage

作     者：Suyan Yao Deyu Zheng Zhuo Liu Ying Jin 

作者机构：Department of Pathophysiology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Anatomy Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Pharmacology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Pharmacology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2006年第1卷第8期

页      面：693-696页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：the Natural Science Foundation of Liaoning Province No.2040987 

主　　题：TNF LDH 

摘      要：BACKGROUND: Sodium ferulate (SF) has an effect of anti-inflammation; however, whether it can inhibit beta-amyloid (Aβ) induced damage or not should be further studied. OBJECTIVE: To investigate the effects of SF on neurotoxicity mediated by Aβ-induced macrophage activation via inhibiting tumor necrosis factor-α (TNF-α) in vitro. DESIGN: A contrast experiment based on cells. SETTING: Departments of Pathophysiology, Pharmacology and Anatomy, Liaoning Medical College. MATERIALS: A total of 36 Kunming mice aged 8-10 weeks and some SD rats aged 2-3 days of both genders were selected in this study. Main reagents were detailed as follows: Aβ peptide (Sigma Company); SF (purity 99%, Suzhou Changtong Chemical Co., Ltd.); lactate dehydrogenase (LDH) assay kit (Bangding Biological Engineering Co., Beijing, China); microtubule-associated protein 2 (MAP-2) monoclonal antibodies and TNF-α monoclonal antibodies (Boster Biological Engineering Co., Wuhan, China). METHODS: The experiment was carried out in Laboratories of Pharmacology and Anatomy, Liaoning Medical College from May to December 2004. Cerebellum was obtained from rats under sterile condition to culture neurons and macrophages taken from mice abdominal cavity. Later, two parallel experiments were performed as follows: ① Macrophages culture groups: In normal control group, macrophages were cultured in DMEM after being seeded. In Aβ group, neurotoxic form of Aβ was added into DMEM media with final concentration of 10 μmol/L after macrophages were seeded for 24 hours. In Aβ+SF group, ten minutes after Aβ treatment, for 10, 100, 500 μmol/L and 1 mmol/L of SF were added to the media of the macrophages culture. ② Macrophages-neurons co-cultured groups: Control macrophages-neurons were co-cultured. Aβ group: Neurotoxic form of Aβ was added into the media with concentration of 10 μmol/L after macrophages were seeded in the neurons cultured wells for 24 hours. Aβ+SF group: Ten minutes after Aβ treatment, 10, 100, 500 μmol/L and