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Growth enhancement effect of BzATP on primary cultured astrocytes from rat brain

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Neuroscience Bulletin 2006年第3期22卷 151-158页

作者： Hua-zheng LIANG ying liu Zhu-Rong YE Department of Pathology Shanghai Medical College Fudan University Shanghai 200032 China

Objective To explore whether BzATP could promote the growth of primary cultured astrocytes （AS） of rat and its possible mechanism, and whether TGF-β1 was involved in the event. Methods The primary cultured AS were derived from new born Sprague-Dawley rats. Glial fibrillary acidic protein （GFAP） immunofluorescent stain was used to check the purity of cultured AS. Morphometry was used to detect the changes of AS. The proliferation index of AS was detected by BrdU incorporation assay. Western blot was used to detect the changes of GFAP under different conditions. Changes of TGF-β1 gene transcription were detected by RT-PCR. ELISA was utilized to detect the variation of TGF-β1 protein in the supernate. Results The purity of primary cultured AS reached to 99%. BzATP promoted the hypertrophy of AS including the elongation of AS processes and the enlargement of cell bodies, BzATP also promoted the expression of GFAP in existence of Ca^2＋, but had no effect on cell proliferation. BzATP increased the transcription of TGF-β1 mRNA and the release of TGF-β1 protein in existence of Ca^2＋. TGF-β1 neutralizing antibody partially inhibited the expression of GFAP induced by BzATP, but had no effect on AS proliferation and cell morphology. Conclusion BzATP enhanced the hypertrophy of primary cultured AS, increased the expression of GFAP partially through TGF-β1. Mechanisms of the enhancement of AS growth induced by BzATP other than TGF-β1 pathway remains to be elucidated.

关键词： astrocyte BzATP Ca^2+ TGF-β1

Inhibition of tumor necrosis factor-alpha by sodium ferulate in protecting neurons from beta-amyloid induced damage

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Neural Regeneration Research 2006年第8期1卷 693-696页

作者： Suyan Yao Deyu zheng Zhuo liu ying Jin Department of Pathophysiology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Anatomy Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Pharmacology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina Department of Pharmacology Liaoning Medical College Jinzhou 121001 Liaoning ProvinceChina

BACKGROUND: Sodium ferulate (SF) has an effect of anti-inflammation; however, whether it can inhibit beta-amyloid (Aβ) induced damage or not should be further studied. OBJECTIVE: To investigate the effects of SF on neurotoxicity mediated by Aβ-induced macrophage activation via inhibiting tumor necrosis factor-α (TNF-α) in vitro. DESIGN: A contrast experiment based on cells. SETTING: Departments of Pathophysiology, Pharmacology and Anatomy, Liaoning Medical College. MATERIALS: A total of 36 Kunming mice aged 8-10 weeks and some SD rats aged 2-3 days of both genders were selected in this study. Main reagents were detailed as follows: Aβ peptide (Sigma Company); SF (purity > 99%, Suzhou Changtong Chemical Co., Ltd.); lactate dehydrogenase (LDH) assay kit (Bangding Biological Engineering Co., Beijing, China); microtubule-associated protein 2 (MAP-2) monoclonal antibodies and TNF-α monoclonal antibodies (Boster Biological Engineering Co., Wuhan, China). METHODS: The experiment was carried out in Laboratories of Pharmacology and Anatomy, Liaoning Medical College from May to December 2004. Cerebellum was obtained from rats under sterile condition to culture neurons and macrophages taken from mice abdominal cavity. Later, two parallel experiments were performed as follows: ① Macrophages culture groups: In normal control group, macrophages were cultured in DMEM after being seeded. In Aβ group, neurotoxic form of Aβ was added into DMEM media with final concentration of 10 μmol/L after macrophages were seeded for 24 hours. In Aβ+SF group, ten minutes after Aβ treatment, for 10, 100, 500 μmol/L and 1 mmol/L of SF were added to the media of the macrophages culture. ② Macrophages-neurons co-cultured groups: Control macrophages-neurons were co-cultured. Aβ group: Neurotoxic form of Aβ was added into the media with concentration of 10 μmol/L after macrophages were seeded in the neurons cultured wells for 24 hours. Aβ+SF group: Ten minutes after Aβ treatment, 10, 100, 500 μmol/L and

关键词： TNF LDH

Self-assembled core-shell polydopamine@MXene with synergistic solar absorption capability for highly efficient solar-to-vapor generation

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Nano Research 2020年第1期13卷 255-264页

作者： Xing Zhao Xiang-Jun Zha Li-Sheng Tang Jun-Hong Pu Kai Ke Rui-ying Bao zheng-ying liu Ming-Bo Yang Wei Yang College of Polymer Science and Engineering Sichuan UniversityState Key Laboratory of Polymer Materials EngineeringChengdu610065China

As a renewable and environment-friendly technology for seawater desalination and wastewater purification,solar energy triggered steam generation is attractive to address the long-standing global water scarcity ***,practical utilization of solar energy for steam generation is severely restricted by the complex synthesis,low energy conversion efficiency,insufficient solar spectrum absorption and water extraction capability of state-of-the-art ***,for the first time,we report a facile strategy to realize hydrogen bond induced self-assembly of a polydopamine(PDA)@MXene microsphere photothermal layer for synergistically achieving wide-spectrum and highly efficient solar absorption capability(≈96%in a wide solar spectrum range of 250–1,500 nm wavelength).Moreover,such a system renders fast water transport and vapor escaping due to the intrinsically hydrophilic nature of both MXene and PDA,as well as the interspacing between core-shell *** solar-to-vapor conversion efficiencies under the solar illumination of 1 sun and 4 sun are as high as 85.2%and 93.6%,***,the PDA@MXene photothermal layer renders the system durable mechanical properties,allowing producing clean water from seawater with the salt rejection rate beyond 99%.Furthermore,stable light absorption performance can be achieved and well maintained due to the formation of ternary TiO2/C/MXene complex caused by oxidative degradation of ***,this work proposes an attractive MXene-assisted strategy for fabricating high performance photothermal composites for advanced solar-driven seawater desalination applications.

关键词： self-assembly polydopamine(PDA)@MXene microsphere synergistic light absorption solar-to-vapor generation light absorption stability

Synthesis of Inorganic Silica Grafted Three-arm PLLA and Their Behaviors for PLA Matrix

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Chinese Journal of Polymer Science 2019年第3期37卷 216-226页

作者： Da-Wei Shi Xiang-Ling Lai Yuan-Ping Jiang Cong Yan zheng-ying liu Wei Yang Ming-Bo Yang State Key Laboratory of Polymer Materials Engineering College of Polymer Science and Engineering Sichuan University

The low melt strength and poor crystallization behavior severely limit the processing and application of poly(lactic acid)(PLA) as biodegradable film materials. In this work, three-arm poly(L-lactic acid)(3A-PLLA) grafted silica nanoparticles with two kinds of topology structures were introduced into PLA matrix to improve the biodegradation performance. Different methods were used to characterize the structure of the grafted 3A-PLLA chains, the grafting density, and the thermal decomposition behavior of the nanoparticles. By varying the mass ratios of raw materials and altering the order of dropping solution in the reaction, high grafting densitytangled 3A-PLLA grafted SiO_2 was synthesized as "3A-PLLA grafting to SiO_2"(GTS), while low grafting density-stretched 3A-PLLA grafted SiO_2 was obtained as "SiO_2 grafting to 3A-PLLA"(GTA). Topology of nanoparticles as well as the filler-matrix interaction is critically important to structure bio-nanocomposites with desirable properties. Thus, the GTS and GTA nanoparticles were introduced into PLA matrix to assess the effect. The SEM images showed the uniform dispersion of the modified nanoparticles, while the shear rheology results revealed that GTA nanoparticles made a more significant contribution on the melt-strengthening and relaxation time-extension of PLA. Moreover, it is suggested that GTA nanoparticles were more effective to act as a nucleating agent for PLA, which was proved by differential scanning calorimetry(DSC) and polarized optical microscopy(POM) researches. All of the improvements mentioned above of GTA nanocomposites can be ascribed to stronger entanglements between 3A-PLLA stretched by nano-SiO_2 and PLA matrix.

关键词： Poly(lactic acid) Nanocomposites Surface grafting Topology Crystallization

Hierarchical Crystalline Structures Induced by Temperature Profile in HDPE Bars during Melt Penetration Process

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Chinese Journal of Polymer Science 2017年第1期35卷 108-122页

作者： chun-xia zhu rui-yan zhang yan-hao huang wei yang zheng-ying liu jian-ming feng 杨鸣波 College of Polymer Science and Engineering State Key Laboratory of Polymer Materials Engineering Sichuan University Chengdu 610065 China

The hierarchical crystalline morphologies and orientation structures across the thickness direction in high-density polyethylene （HDPE） molded bars were investigated via a novel melt-penetrating processing method named multi-melt multi-injection molding （M3IM）. The samples with various mold temperatures （20, 40 and 60 ℃） were prepared, and the effects of the external temperature profile on the evolution of crystalline microstructures were studied. With scanning electron microscopy （SEM）, the transition of crystalline morphology from ring-banded structure to oriented lamellae was observed with decreasing mold temperature, and the oriented lamellae were formed at the sub-skin layer of the samples at the lowest mold temperature, which was further testified by differential scanning calorimetry （DSC）. With the decline of mold temperature, the degree of orientation, obtained from two-dimensional small angle X-ray scattering （2D-SAXS）, was increased and long periods rose a little. Thus, decreasing mold temperature was beneficial to the formation of orientation structures because the relaxation of chains was weakened.

关键词： Hierarchical structures Orientation Multi-melt multi-injection molding Mold temperature

Potential association of hyperhomocysteinemia with the progression of IgA nephropathy： a retrospective study

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Chinese Medical Journal 2014年第10期127卷 1849-1852页

作者： Duan Shuwei liu Shuwen Sun Xuefeng zheng ying liu Linchang Yao Feixiang Wu Jie Department of Nephrology Chinese People's Liberation Army General Hospital (301 Hospital) Chinese People's Liberation Army Institute of Nephrology State Key Laboratory of Kidney Diseases (2011DAV00088) National Clinical Research Center of Kidney Diseases (2013BAI09B05) Beijing 100853 China

Background The high blood homocysteine （Hcy） levels found in patients with hyperhomocysteinemia （HHcy） have been implicated in an increased risk of cardiovascular disease morbidity and mortality in end-stage renal disease （ESRD）.This study investigated the association of HHcy with progression of IgA *** We analyzed 108 participants newly diagnosed with IgA nephropathy between August 2005 and August 2007 in the Department of Nephrology,Chinese People＇s Liberation Army General *** association between clinicopathological factors and the Hcy levels were analyzed by Logistic regression and those with ESRD risk were analyzed by Cox *** Patients were aged （35.71±10.73） years and included 45.71％ women and 12.04％ patients with *** multivariate Logistic regression analysis,HHcy was associated with arterial lesions （OR 2.60; 95％ CI 1.55-4.34; P＜0.001） even when age,body mass index,estimated glomerular filtration rate,mean arterial pressure,and initial proteinuria were taken into *** follow-up was （67.37±16.21） *** was also associated with worse ESRD-free survival （HR 4.71; 95％ CI 1.45 to 15.31; P=0.010）.Conclusion HHcy is associated with the risk of intrarenal arterial lesions and may be useful for estimating the prognosis of IgA nephropathy.

关键词： IgA nephropathy hyperhomocysteinemia prognosis

Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng zheng Wang Zhou ying Mo Biao zheng Miaomiao Han Qin Zhong Wenwen Yang Peng Gao Lezhi Yang Jilei liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater Bay Area Institute for Innovation Hunan University Changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the Na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more Na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced Na+storage performance.

关键词： X ray photoelectron spectroscopy

Effect of DPC4 Gene on Invasion and Metastasis of Colorectal Carcinoma Cells

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 883-892页

作者： De-Sheng XIAO Ji-Fang WEN* Jing-He LI Kuan-Song WANG Zhong-Liang HU Jian-Hua ZHOU zheng-Hao DENG ying liu Department of Pathology,Xiangya School of Medicine,Central South University,Changsha 410078,China Department of Pathology Xiangya School of Medicine Central South UniversityChangsha 410078 China

To investigate the effect of DPC4 gene on invasion and metastasis of colorectal carcinomacells,the expression of DPC4 was detected in sixty-three samples of colorectal tumors and seven cases ofcolorectal *** biological behavior of tumors expressing DPC4 was evaluated (including tumorstaging,differentiation degree and metastasis).pcDNA3.1-DPC4 plasmid was constructed and transferredinto HCT116 cells not expressing *** cell models (DPC4~+-HCT116) steadily expressing DPC4 *** with HCT116 and pcDNA3.1-HCT116 cells,the doubling time of DPC4~+-HCT116 cellswas lengthened obviously (P<0.01),the apoptosis rate of DPC4~+-HCT 116 cells was significantly increased(P<0.01),the cloning efficiency,cell adherency,migration and invasion ability of DPC4~+-HCT116 cells weredropped obviously (P<0.01).The number of cancer nodules was decreased significantly in abdominal cavityand liver of the nude mice inoculated with DPC4~+-HCT116 *** activity of MMP-9 and MMP-2 wasdetected by gelatin *** comparison with HCT116 and pcDNA3.1-HCT116 cells,the activity ofMMP-9 was decreased in DPC4~+-HCT116 ***,the down-regulation of DPC4 expression may beassociated with the carcinogenesis of colorectal ***4 may inhibit the proliferation of coloncancer cell by restraining growth and inducing apoptosis,and the invasion and metastasis of colorectalcarcinoma ***-9 may be one of the downstream target genes regulated by DPC4.

关键词： DPC4 colorectal carcinoma invasion metastasis MMP9

NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao Lin Changzhou ying Xiaoli Si Naijia Xue Yi liu Ran zheng ying Chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether NADPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased NADPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,NADPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that NADPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,NADPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that NADPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that NADPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis NADPH oxidase 4(NOX4) neuroinflammation Parkinson's disease