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Acta Pharmacologica Sinica 2005年第8期26卷 982-986页

作者： Cai-ping MAO, zhen-lun gu~1 Department of Pharmacology, Suzhou Institute of Chinese Materia Medica, Medical College of Soochow University Suzhou 215007, China Department of Pharmacology Suzhou Institute of Chinese Materia Medica Medical College of Soochow University Suzhou China

Aim: Increased expression of c-fos, c-jun and type Ⅳ collagen (CoⅣ) in glomerular mesangial cells (GMC) are important characteristics of diabetic nephropathy. Both c-los and c-jun regulate the gene expression of extracellular matrix components, and CoⅣ is the main component of the extracellular matrix. It has been reported that puerarin inhibits aggregation of the extracellular matrix in diabetic rats by an as yet unknown mechanism. The aim of this study is to investigate the effect of puerarin on c-fos, c-jun and CoⅣ expression in GMC cultured in medium containing 5.6 or 27.8 mmol/L glucose. Methods: The expressions of c-fos and c-jun were measured at the protein level using flow cytometry. CoⅣ content was detected using radioimmunoassay. Protein kinase C (PKC) activity was measured using liquid scintillation counting. Results: Puerarin (10-5mmol/L) significantly ameliorated the high-glucose effect on c-fos, c-jun and CoⅣ expression. This effect is accompanied by a reduced PKC activity in these cells. Coclusion: Our results suggest that reduced PKC activity and expression of c-fos and c-jun in GMC might participate in the mechanisms underlying the therapeutic effect of puerarin on diabetic nephropathy.

关键词： puerarin glomerular mesangial cells extracellular matrix diabetic nephropathy

Effect of Astragalus complanatus flavonoid on anti-liver fibrosis in rats

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World Journal of Gastroenterology 2005年第37期11卷 5782-5786页

作者： Chun-Yu Liu zhen-lun gu Wen-Xuan Zhou Ci-Yi guo Department of Pharmacology Medical College of Suzhou University Suzhou 215007 Jiangsu Province China Suzhou Institute of Chinese Meteria Medica Suzhou University Suzhou 215007 Jiangsu Province China The Hong Kong Association for Health Care Ltd Hong Kong China

AIM： To observe the anti-liver fibrosis effect of Astragalus complanatus fiavonoids （ACF） in rats. METHODS： The liver fibrosis model in rats was established by injecting interperitoneally 0.2 mL/100 g 0.5% dimethylnitrosamine, thrice a week. Meanwhile, the rats were administered ACF （30, 60, 120 mg/kg） or colchicine （0.1 mg/kg） once a day for 1 mo. Serum N-propeptide of type Ⅰ procollagen （PINP） and type Ⅲ procollagen （PⅢNP） was measured using ELISA. Malondialdehyde （MDA） and superoxide dismutase （SOD） in hepatic tissue were evaluated. Matrix metal protease-1 （MMP-1） mRNA expression was assayed by RT-PCR and the protein expression of tissue inhibitor of metal protease-1 （TIMP-1） was analyzed by immunohistochemistry. RESULTS： In the ACF groups, SOD activity increased and MDA content decreased in comparison to the liver fibrosis model group. The serum PINP and PⅢNP contents in ACF-2 and -3 group decreased compared to those in model group. In ACF-2 and -3 group, the expression of MMP-1 mRNA increased significantly and the protein expression of TIMP-1 decreased compared to that in model group. CONCLUSION： The antifibrotic mechanisms of ACF are associated with its influence on lipid peroxidation and collagen synthesis and degradation.

关键词： Astragalus complanatus, Liver fibrosis N-propeptide of typeⅠ procollagen N-propeptide of type Ⅲ procollagen Malondialdehyde Superoxide dismutase Matrix metal protease-1 Tissue inhibitor of metal protease-1

Mitochondrial dysfunction and Huntington disease

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Neuroscience Bulletin 2006年第2期22卷 129-136页

作者： Wei-Yan ZHANG zhen-lun gu Zhong-Qin LIANG zheng-Hong QIN Soochow University School of Life Sciences Department of Pharmacology Soochow University School of Medicine Suzhou 215123 China

Huntington disease （HD） is a chronic autosomal-dominant neurodegenerative disease. The gene coding Huntingtin has been identified, but the pathogenic mechanisms of the disease are still not fully understood. This pa... 详细信息

关键词： mitochondrial dysfunction Huntington disease Huntingtin

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Experimental study of osthole on treatment of hyperlipidemic and alcoholic fatty liver in animals

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World Journal of Gastroenterology 2006年第27期12卷 4359-4363页

作者： Fang Song Mei-Lin Xie Lu-Jia Zhu Ke-Ping Zhang Jie Xue zhen-lun gu Department of Pharmacology Medical School of Soochow University Suzhou 215123 Jiangsu Province China Suzhou Institute of Chinese Marteria Medica Suzhou 215123 Jiangsu Province China

AIM： To evaluate the effects of osthole on fatty liver, and investigate the possible mechanism. METHODS： A quail model with hyperlipidemic fatty liver and rat model with alcoholic fatty liver were set up by feeding high fat diet and alcohol, respectively. These experimental animals were then treated with osthole 5-20 mg/kg for 6 wk, respectively. Whereafter, the lipid in serum and hepatic tissue, and coefficient of hepatic weight were measured. RESULTS： After treatment with osthole the levels of serum total cholesterol （TC）, triglyceride （TG）, lower density lipoprotein-cholesterol （LDL-C）, coefficient of hepatic weight, and the hepatic tissue contents of TC and TG were significantly decreased. The activity of superoxide dismutase （SOD） in liver was improved. In alcohol-induced fatty liver rats, the level of malondialdehyde （MDA） in liver was decreased. In high fat-induced fatty liver quails, glutathione peroxidase （GSH-PX） in liver was significantly improved. The histological evaluation of liver specimens demonstrated that the osthole dramatically decreased lipid accumulation. CONCLUSION： These results suggested that osthole had therapeutic effects on both alcohol and high fatinduced fatty liver. The mechanism might be associated with its antioxidation.

关键词： Hyperlipidemic fatty liver Alcoholic fatty liver Osthole Quails Rats

Anti-explosion performance and dynamic response of an innovative multi-layer composite explosion containment vessel

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Defence Technology（防务技术） 2024年第6期36卷 105-121页

作者： zhen Wang Heng Chen Qi Yuan Wenbin gu Xingbo Xie Hongwei Li College of Field Engineering Army Engineering University of PLANanjing 210007China College of Civil Engineering and Architecture Southwest University of Science and TechnologySichuanMianyang 621010China

An innovative multi-layer composite explosion containment vessel(CECV)utilizing a sliding steel platealuminum honeycomb-fiber cloth sandwich is put forward to improve the anti-explosion capacity of a conventional single-layer explosion containment vessel(SECV).Firstly,a series of experiments and finite element(FE)simulations of internal explosions are implemented to understand the basic anti-explosion characteristics of a SECV and the rationality of the computational models and methods is verified by the comparison between the experimental results and simulation *** on this,the CECV is designed in detail and a variety of FE simulations are carried out to investigate effects of the sandwich structure,the explosive quantity and the laying mode of the fiber cloth on anti-explosion performance and dynamic response of the CECV under internal *** results indicate that the end cover is the critical position for both the SECV and *** maximum pressure of the explosion shock wave and the maximum strain of the CECV can be extremely declined compared to those of the *** a result,the explosive quantity the CECV can sustain is up to 20 times of that the SECV can ***,as the explosive quantity increases,the internal pressure of the CECV keeps growing and the plastic deformation and failure of the sandwich structure become more and more severe,yielding plastic strain of the CECV in addition to elastic *** results also reveal that the laying angles of the fiber cloth's five layers have an impact on the anti-explosion performance of the *** example,the CECV with fiber cloth layered in 0°/45°/90°/45°/0°mode has the optimal anti-capacity,compared to 0°/0°/0°/0°/0°and 0°/30°/60°/30°/0°***,owing to remarkable anti-explosion capacity,this CECV can be regarded as a promising candidate for explosion resistance.

关键词： Explosive container Honeycomb-fiber cloth Anti-explosion Aluminum honeycomb core Composite

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Therapeutic effect of osthole on hyperlipidemic fatty liver in rats

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Acta Pharmacologica Sinica 2007年第3期28卷 398-403页

作者： Yan ZHANG Mei-lin XIE~2 Lu-jia ZHU zhen-lun gu Department of Pharmacology,Medical School of Soochow University Suzhou 215123,China DepartmentofPharmacology MedicalSchoolofSoochowUniversitySuzhou215123China

Aim:To study the effects of osthole on hyperlipidemic fatty liver and investigatethe possible ***:A rat model with hyperlipidemic fatty liverwas successfully established by feeding fatty milk for 6 *** experimentalrats were then treated with 5-20 mg/kg osthole for 6 *** mouse hyperlipi-demic model was induced by feeding fatty milk when they were treated with 10-20mg/kg osthole for 3 ***:After treatment with osthole,the levels of ratserum total cholesterol(TC),triglyceride(TG)and low density lipoprotein-choles-terol significantly decreased as compared with the fatty liver model group(P<0.05or P<0.01).Hepatic weight and its coefficient,the hepatic tissue contents of TC,TG,and malondialdehyde,also significantly decreased(P<0.05 or P<0.01).Infatty milk-induced hyperlipidemic mice,the post-heparin plasma activities of lipo-protein lipase(LPL),hepatic lipase(HL),and total lipase(TL)significantlyincreased after treatment with 10-20 mg/kg osthole for 3 weeks(P<0.05 or P<0.01).Importantly,the histological evaluation of rat liver demonstrated that ostholedramatically decreased lipid accumulation(P<0.01).Conclusion:Osthole wasfound to have therapeutic effects on fatty milk-induced rat fatty liver;the mecha-nisms might be associated with its anti-oxidation and the elevation of the activi-ties of LPL and HL.

关键词： osthole hyperlipidemic fatty liver pharmacology

Caspase-1 inhibitor Ac-YVAD-CHO attenuates quinolinic acid-induced increases in p53 and apoptosis in rat striatum

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Acta Pharmacologica Sinica 2005年第2期26卷 150-154页

作者： Yi CAO zhen-lun gu Fang LIN Rong HAN zheng-hong QIN Department of Pharmacology,Soochow University School of Medicine,Suzhou 215007,China Department of Pharmacology Soochow University School of Medicine Suzhou 215007 China

Aim:To study the effects of the caspase-1 inhibitor Ac-YVAD-CHO on quinolinicacid(QA)-induced ***:Rats were pre-treated with intrastriatalinfusion of Ac-YVAD-CHO(2-8μg)before intrastriatal injection of QA(60 nmol).Striatal total proteins,genomic DNA,and nuclear proteins were *** of Ac-YVAD-CHO on QA-induced caspase-1 activity,internucleosomalDNA fragmentation, IκB-α degradation,NF-κB,and AP-1 activation,and increasesin p53 protein levels were measured with enzyme assays,agarose gel electro-phoresis,electrophoresis mobility shift assays,and Western blot ***:Pre-treatment with Ac-YVAD-CHO inhibited QA-induced internucleo-somal DNA ***-YVAD-CHO inhibited QA-induced increases incaspase-1 activity and p53 protein levels,but had no effect on QA-induced IκB-αdegradation,NF-κB or AP-1 ***:Caspase-1 is involved inQA-induced p53 upregulation but not IκB-α *** of caspase-1attenuates QA-induced apoptosis in rat striatum.

关键词： caspase 1 Ac-YVAD-CHO Huntington disease protein p53 NF-kappaB inhibitor alpha apoptosis NF-kappaB

Prostaglandin A1 inhibits increases in intracellular calcium concentration,TXA2 production and platelet activation

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Acta Pharmacologica Sinica 2006年第5期27卷 549-554页

作者： Yi ZHU zhen-lun gu Zhong-qin LIANG Hui-lin ZHANG zheng-hong QIN~2 Department of Pharmacology,Soochow University School of Medicine,Suzhou 215123,China DepartmentofPharmacology SoochowUniversitySchoolofMedicineSuzhou215123China

Aim:In our previous studies we found that cyclopentenane prostaglandin A1(PGA1) had neuroprotective effects in a rodent ischemic *** the presentstudy we aimed to investigate the inhibitory effect of PGA1 on platelet ***:The rate of aggregation of human platelets was measured by *** rate of adhesion of platelets to cultured endothelial cells wasdetermined by using [~3H]-adenine labeled platelets.5-Hydroxytryptamine releasefrom platelets was measured with O-phthaldialdehyde *** levels of TXB2,a stable metabolite of TXA2,were determined by *** in platelet morphology were observed using an electronmicroscope,and the intraplatelet free calcium concentrations were measured withFluo-3/AM FCM ***:PGA1 significantly inhibited thrombin-,col-lagen-and ADP-induced aggregation and adhesion of *** morphologi-cal changes of platelets induced by thrombin were blocked by ***1inhibited the release of 5-hydroxytyptamine from dense granules and the synthe-sis of ***:PGA1 inhibits the activation of platelets probablythrough blocking increases in intracellular calcium concentration and TXA2synthesis.

关键词： prostaglandin A1 platelet aggregation calcium TXA2

Waldenström macroglobulinemia:a challenging case treated with anti-CD19 CAR-T cell therapy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第8期25卷 719-722页

作者： Yang YANG Xiaolin gu Jingsong HE Yongxian HU zhen CAI Bone Marrow Transplantation Center Department of Hematologythe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310006China Department of Hematology Putuo People’s HospitalZhoushan 316100China

Waldenström macroglobulinemia(WM)is characterized by lymphoplasmacytic lymphoma associated with large amounts of monoclonal immunoglobulin M(IgM)protein(Owen et al.,2003).Common signs and symptoms include fatigue due to anemia,lymph node enlargement,hepatosplenomegaly,thrombocytopenia,symptoms related to high viscosity,and peripheral neuropathy,among *** significant advances in WM treatment,this type of indolent lymphoma remains incurable,with a wide array of patient outcomes(Ruan et al.,2020).In recent years,chimeric antigen receptor T(CAR-T)cell therapy targeting cluster of differentiation 19(CD19)has shown unprecedented response rates and durability in the treatment of B-cell *** this report,we describe a challenging case of WM that involved multiple extramedullary sites,relapsed,and was refractory to chemotherapy,immunotherapy,and targeted *** anti-CD19 CAR-T cell therapy,the tumor burden significantly decreased and the patient’s condition remained stable at the writing of this report.

关键词： CD19 globulin durability

Dynamic Response of A Group of Cylindrical Storage Tanks with Baffles Considering the Effect of Soil Foundation

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China Ocean Engineering 2024年第1期38卷 129-143页

作者： SUN Ying WANG Jia-dong HUO Rui-li ZHOU Ding gu zhen-yuan QIAN Wang-ping School of Transportation and Civil Engineering Nantong UniversityNantong 226019China Faculty of Civil Engineering and Mechanics Jiangsu UniversityZhenjiang 212013China College of Civil Engineering Nanjing Tech UniversityNanjing 211816China

The sloshing in a group of rigid cylindrical tanks with baffles and on soil foundation under horizontal excitation is studied *** solutions for the velocity potential are derived out by the liquid subdomain *** models with mass-spring oscillators are established to replace continuous *** with the least square technique,Chebyshev polynomials are employed to fit horizontal,rocking and horizontal-rocking coupling impedances of soil,respectively.A lumped parameter model for impedance is presented to describe the effects of soil on tank structures.A mechanical model for the soil-foundation-tank-liquid-baffle system with small amount of calculation and high accuracy is proposed using the substructure *** analytical solutions are in comparison with data from reported literature and numerical codes to validate the effectiveness and correctness of the *** dynamic properties and seismic responses of the soil-tank system are given for the baffle number,size and location as well as soil parameter.

关键词： cylindrical tanks multiple annular baffles equivalent analytical model soil−structure interaction subdomain method dynamic response

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