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Dendrobium species regulate energy homeostasis in neurodegenerative diseases: a review

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Food Science and Human Wellness 2023年第6期12卷 2151-2174页

作者： Feixuan Wang Jingqiong Wan Yangzhen Liao Shangyu Liu Yuan Wei zhen ouyang School of Pharmacy Jiangsu UniversityZhenjiang 212013China Nanjing Institute of Product Quality Inspection Nanjing 210019China School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013China

Brain energy homeostasis is a vital physiological function in maintaining a balanced internal metabolic environment.The impairment of energy homeostasis is recognized as a key pathophysiological basis for brain metabolic disorders and related neurodegenerative diseases.Dendrobium species(‘Shihu’in Chinese)such as D.officinale,D.huoshanense,D.nobile,D.chrysanthum,D.loddigesii,D.moniliforme,D.gratiosissimum,D.candidum and D.caulis are widely used as traditional Chinese medicines/nutraceuticals to control and treat neurodegenerative disorders.These dietary herbs and their derived compounds possess a variety of biological properties,such as suppression of oxidative stress and neuroinflammation,regulation of energy homeostasis mainly through improving brain mitochondria function,insulin signaling and lipid metabolism.Furthermore,they reduce neurotoxicity,alleviate brain injury and neuropathy,and prevent neurodegenerative conditions including stroke,Alzheimer’s disease,Parkinson’s disease,and Huntington’s disease in humans and/or rodents.Moreover,the nutraceuticals from Dendrobium species promote gut health and aid digestion,which appear to be associated with beneficial effects on brain energy homeostasis.Based on the above-mentioned health benefits associated with Dendrobium species,this work reviews their nutraceutical role in neurodegenerative disorders and further suggests the need to elucidate mechanisms of the underlying molecular actions.

关键词： Dendrobium species Nutraceutical role Energy homeostasis Neurodegenerative diseases

Amelioration of ethanol-induced oxidative stress and alcoholic liver disease by in vivo RNAi targeting Cyp2e1

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药学学报:英文版 2023年第9期13卷 3906-3918页

作者： Yalan Wang Qiubing Chen Shuang Wu Xinyu Sun Runting Yin zhen ouyang Hao Yin Yuan Wei School of Pharmacy Jiangsu UniversityZhenjiang 212013China Department of Urology Frontier Science Centre for Immunology and MetabolismMedical Research InstituteZhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China Department of Pulmonary and Critical Care Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Pathology Zhongnan Hospital of Wuhan UniversityWuhan 430071China RNA Institute Wuhan UniversityWuhan 430072China Wuhan Research Centre for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430010China

Alcoholic liver disease(ALD)results from continuous and heavy alcohol consumption.The current treatment strategy for ALD is based on alcohol withdrawal coupled with antioxidant drug intervention,which is a long process with poor efficacy and low patient compliance.Alcohol-induced CYP2E1 upregulation has been demonstrated as a key regulator of ALD,but CYP2E1 knockdown in humans was impractical,and pharmacological inhibition of CYP2E1 by a clinically relevant approach for treating ALD was not shown.In this study,we developed a RNAi therapeutics delivered by lipid nanoparticle,and treated mice fed on Lieber-DeCarli ethanol liquid diet weekly for up to 12 weeks.This RNAi-based inhibition of Cyp2e1 expression reduced reactive oxygen species and oxidative stress in mouse livers,and contributed to improved ALD symptoms in mice.The liver fat accumulation,hepatocyte inflammation,and fibrosis were reduced in ALD models.Therefore,this study suggested the feasibility of RNAi targeting to CYP2E1 as a potential therapeutic tool to the development of ALD.

关键词： Alcoholic liver disease RNAi Lipid nanoparticle Cyp2e1 Reactive oxygen species Oxidative stress Lipogenesis Lipometabolism

Generation of pigs with humanized type Ⅱ collagen by precise human COL2A1 gene knock-in

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Journal of Genetics and Genomics 2023年第3期50卷 212-215页

作者： Ting Lan Yuling zheng Yangyang Suo Yuhui Wei Hui Shi Quanmei Yan zhenpeng Zhuang Huangyao Chen Quanjun Zhang Nana Fan Yu Zhao zhen ouyang Chengdan Lai Zhaoming Liu Jizeng Zhou Chengcheng Tang Nam-Hyung Kim Qingjian Zou Xiaomin Wang Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesWuyi UniversityJiangmenGuangdong 529020China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhouGuangdong 510530China Joint School of Life Science Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhouGuangdong 510530China The School of Biomedical and Pharmaceutical Sciences Guangdong University of TechnologyGuangzhouGuangdong 510006China School of Life Sciences Westlake UniversityHangzhouZhejiang 310024China Institute of Biology Westlake Institute for Advanced StudyHangzhouZhejiang 310024China

Osteoarthritis(OA)is the most common chronic disease,characterized by progressive cartilage breakdown,subchondral bone sclerosis,and aberrant bone outgrowth(Yucesoy et al.,2015;Hussain et al.,2016).OA is one of the leading causes of cartilage damage.Patients with severe cartilage damage require transplantation of articular cartilage to improve their quality of life.Type Ⅱ collagen is a major component of articular cartilage and intervertebral discs and plays an important role in the structure and strength of connective tissues that support muscles and joints(Byers,1994).

关键词： damage cartilage

Production of transgenic pigs over-expressing theantiviral gene Mx1

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Cell Regeneration 2014年第1期3卷 76-86页

作者： Quanmei Yan Huaqiang Yang Dongshan Yang Bentian Zhao zhen ouyang Zhaoming Liu Nana Fan Hongsheng ouyang Weiwang Gu Liangxue Lai Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhouChina College of Animal Sciences Jilin UniversityChangchunChina Institute of Comparative Medicine and Center of Laboratory Animals Southern Medical UniversityGuangzhouChina

The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interestingcandidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cellnuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs expressapproximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was alsomarkedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs withinfluenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profounddecrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-foldreduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found thatthe Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These resultsdemonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate thatthe Mx1 transgene can be harnessed to develop disease-resistant pigs.

关键词： Antiviral breeding Innate resistance Somatic cell nuclear transfer

Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression

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Science China(Life Sciences) 2022年第11期65卷 2269-2286页

作者： Qin Jin Xiaoyu Yang Shixue Gou Xiaoyi Liu zhenpeng Zhuang Yanhui Liang Hui Shi Jiayuan Huang Han Wu Yu Zhao zhen ouyang Quanjun Zhang Zhaoming Liu Fangbing Chen Weikai Ge Jingke Xie Nan Li Chengdan Lai Xiaozhu Zhao Jiaowei Wang Meng Lian Lei Li Longquan Quan Yinghua Ye Liangxue Lai Kepin Wang CAS Key Laboratory of Regenerative Biology Joint School of Life SciencesGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou 510530China Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine Centre for Regenerative Medicine and HealthHong Kong Institute of Science and InnovationGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Sanya institute of Swine resource Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesHainan Provincial Research Center of Laboratory AnimalsSanya 572000China Research Unit of Generation of Large Animal Disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou 510005China Guangdong Provincial Key Laboratory of Laboratory Animals Guangdong Laboratory Animals Monitoring InstituteGuangzhou 510633China Guangdong Provincial Key Laboratory of Large Animal models for Biomedicine Wuyi UniversityJiangmen 529020China

Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process.Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline.Previous pig models with tetracycline regulatory elements were generated through random integration.This process often resulted in uncertain expression and unpredictable phenotypes,thus hindering their applications.Here,by precise knock-in of binary Tet-On 3G elements into Rosa26 and Hipp11 locus,respectively,a double knock-in reporter pig model was generated.We characterized excellent properties of this system for controllable transgenic expression both in vitro and in vivo.Two att P sites were arranged to flank the td Tomato to switch reporter gene.Single or multiple gene replacement was efficiently and faithfully achieved in fetal fibroblasts and nuclear transfer embryos.To display the flexible application of this system,we generated a pig strain with Dox-inducing h KRASexpression through phiC31 integrase-mediated cassette exchange.After eight months of Dox administration,squamous cell carcinoma developed in the nose,mouth,and scrotum,which indicated this pig strain could serve as an ideal large animal model to study tumorigenesis.Overall,the established pig models with controllable and switchable transgene expression system will provide a facilitating platform for transgenic and biomedical research.

关键词： double knock-in Tet-On system phiC31-mediated cassette exchange pig model hKRASG12D-related tumorigenesis

Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation

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Journal of Genetics and Genomics 2020年第5期47卷 249-261页

作者： Xiaomin Wang Hui Shi Juanjuan Zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo zhen ouyang Chengdan Lai Quanmei Yan Liangxue Lai CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou510530China University of Chinese Academy of Sciences Beijing100049China Guangzhou Regenerative Medicine and Health Guangdong Laboratory(GRMH-GDL) Guangzhou510005China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing100101China Research Unit of Generation of Large Animal Disease Models Chinese Academy of Medical Sciences(2019RU015)Guangzhou510530China School of Biotechnology and Health Sciences Wuyi UniversityJiangmen529020China Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational MedicineShenzhen University Health Science CenterShenzhen University School of MedicineFirst Affiliated Hospital of Shenzhen UniversityShenzhen Second People's HospitalShenzhen518035China Department of Central Laboratory Shenzhen Longhua District Central HospitalShenzhen518110China School of Life Sciences University of Science and Technology of ChinaHefei230027China Joint School of Life Sciences Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhou511436China

Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted organs.Therefore,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is necessary.In this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1 protein,which is associated with endothelial and hematopoietic cell development.We found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse chimeras.The whole yolk sac(YS)of Flk-1^EGFP/ECFP rat-mouse chimera was full of rat blood vasculature.Rat genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the YS.Our results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse.

关键词： Blastocyst complementation Interspecies chimera Intraspecies chimera Flk-1 Vascular endothelial cell Hematopoietic cell

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改良HPLC法测定利可君含量

山东化工 2024年第4期53卷 156-158,162页

作者：董飞欧阳臻张小平陈进曹旭江苏大学药学院江苏镇江212013 江苏吉贝尔药业股份有限公司江苏镇江212009

目的:建立一种改良后的HPLC法用于利可君含量测定。方法:采用高效液相色谱法,色谱柱:赛默飞C 18(4.6 mm×100 mm,3μm),流动相:水-乙腈-冰醋酸(体积比75∶25∶0.3),等度洗脱,柱温:30℃,检测波长:210 nm,进样量:20μL,流速:1.0 mL/min。... 详细信息

目的:建立一种改良后的HPLC法用于利可君含量测定。方法:采用高效液相色谱法,色谱柱:赛默飞C 18(4.6 mm×100 mm,3μm),流动相:水-乙腈-冰醋酸(体积比75∶25∶0.3),等度洗脱,柱温:30℃,检测波长:210 nm,进样量:20μL,流速:1.0 mL/min。结果:利可君的四个非对映异构体在HPLC中能全部检出且分离彻底,利可君在11.78~294.50μg/mL范围内线性良好,精密度、重现性优,平均回收率为98.48%。结论:本方法专属性好、灵敏度高、分离度高、理论塔板数高,适用于利可君的含量测定。

关键词：改良利可君含量测定高效液相色谱法

Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering

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分子细胞生物学报:英文版 2016年第2期8卷 174-177页

作者： Yi Yang Kepin Wang Han Wu Qin Jin Degong Ruan zhen ouyang Bentian Zhao Zhaoming Liu Yu Zhao Quanjun Zhang Nana Fan Qishuai Liu Shimei Guo Lei Bu Yong Fan Xiaofang Sun Xiaoping Li Liangxue Lai Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China School of Life Sciences University of Science and Technology of ChinaHefei 230027China Leon H Charney Division of Cardiology New York University School of Medicine522 First AvenueNew YorkNY 10016USA Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education InstitutesThe Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150China Jilin Provincial Key Laboratory of Animal Embryo Engineering Institute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchun 130062China

Dear Editor,Type1diabetes(T1D)isa lifelong(chronic)disease and a major health problem throughout the world.This disease can be treatedby either insulin injection or islet transplantation.Islet transplantation is considered as a better treatment for T1D patients,because islets can produce and release insulin at the appropriate time,resulting in tight blood glucose control.

关键词： islet injection expressing

基于TCN网络的调相机局部放电智能检测分析

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现代工业经济和信息化 2024年第1期14卷 136-137,140页

作者：王浩钰欧阳震陈小军白文星张琪张德祯国网上海电力公司特高压换流站分公司上海200126

为了进一步提高调相机缺陷识别能力,设计了一种基于TCN网络的调相机局部放电智能检测方法。在调相机设备上开展了干扰测试,构建能够满足现场调相机内局部放电检测系统。研究结果表明:设计相位反馈控制能够对低于1.8 k Hz低频噪声造成相... 详细信息

为了进一步提高调相机缺陷识别能力,设计了一种基于TCN网络的调相机局部放电智能检测方法。在调相机设备上开展了干扰测试,构建能够满足现场调相机内局部放电检测系统。研究结果表明:设计相位反馈控制能够对低于1.8 k Hz低频噪声造成相位衰落起到明显抑制效果。调相机局部放电检测试验结果显示,10 s时间内,Michelson光纤干涉系统对内部缺陷信号达到了精确检测的效果,PZT传感器则没有检测出内部缺陷的信号。

关键词：气体绝缘开关内部缺陷相位反馈智能控制超声检测