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Journal of Ocean University of China 2015年第4期14卷 685-690页

作者： LIAN Shanshan HE Yan LI Xue zhao.Bosong HOU Rui HU Xiaoli ZHANG Lingling BAO zhenmin Key Laboratory of Marine Genetics and Breeding(MGB) of Ministry of Education College of Marine Life Sciences Ocean University of China

DNA methylation is an important epigenetic regulatory mechanism that influences genomic stability, gene activation, X-chromosome inactivation and other factors. A change in DNA methylation is usually associated with aging and cellular senescence. DNA methyltransferase 1(DNMT1) is the most abundant DNA methyltransferase, and it plays an important role in maintaining the established methylation pattern during DNA replication in vertebrates. Although the effect of aging on DNA methylation has been well studied in vertebrates, little research has been conducted in invertebrates, especially in marine bivalves. In this study, we examined global DNA methylation levels in four groups of adult Zhikong scallop Chlamys farreri at different ages. The results showed that both the age and tissue type had a strong effect on the DNA methylation. In addition, a significant decrease in DNA methylation with aging(1–4 years) can be detected in mantle, kidney and hepatopancreas. We further measured the change in DNMT1 transcript abundance using quantitative reverse transcription PCR(q RT-PCR), which revealed that DNMT1 transcription significantly decreased with aging in mantle and hepatopancreas and strongly correlated with DNA methylation(R = 0.72). Our data provided greater insight into the aging-related decline of DNA methylation, which could aid in gaining a better understanding of the relationship between DNA methylation and the aging process in bivalve mollusks.

关键词： aging DNA methylation DNMT1 transcription Chlamysfarreri

Factors influencing myogenic differentiation of adipose-derived stem cells and their application in muscle regeneration

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Chinese Journal of Plastic and Reconstructive Surgery 2022年第3期4卷 126-132页

作者： Na Liu Guanhuier Wang Yonghuan Zhen Yujia Shang Fangfei Nie Lingjuan Zhu zhenmin zhao.Yang An Department of Plastic Surgery Peking University Third HospitalBeijing 100191China School of Traditional Chinese Materia Medica Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of EducationShenyang Pharmaceutical UniversityShenyang 110016China

Skeletal muscle regeneration mainly depends on muscle satellite cells;however,these cells are not sufficient for supporting repair and regeneration in volumetric muscle loss(VML),Duchenne muscular dystrophy,and other muscle injuries or muscle *** such,much work has been conducted in recent years to search for myogenic stem ***-derived stem cells(ADSCs)have a wide range of sources,rapid growth,and multi-directional differentiation potential,and have become vital candidates for muscle *** factors influence the myogenic differentiation capacity of *** paper reviews the regulatory aspects and possible factors that have been identified in recent years to affect myogenic differentiation of *** on these factors,gene editing,and perfusion concepts,a method was proposed to achieve maximal differentiation efficiency of *** study focused on the application of ADSCs in muscle regeneration and *** on the importance of myogenic differentiation of ADSCs for the repair and regeneration of muscle damage,this study provides a basis for future research surrounding the efficient induction of myogenic differentiation of ADSCs in vitro.

关键词： Adipose-derived stem cells Muscle regeneration Muscle diseases Myogenic differentiation Regulatory factors

Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups

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Forensic Sciences Research 2018年第2期3卷 138-144页

作者： Xiang Sheng Yali Wang Jiashuo Zhang Liqin Chen Yuan Lin zhenmin zhao.Chengtao Li Suhua Zhang Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceShanghaiChina Department of Forensic Medicine Medical College of Soochow UniversitySuzhouChina Department of Forensic Medicine Inner Mongolia Medical UniversityHohhotChina

A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and *** genotyping of DNA samples was consistent across a large range of template DNA concentrations,with complete profiles obtained at 0.125 ng;however,no more than 2 mm× 1.2 mm punches of samples would be recommended for direct *** size precision and accuracy test revealed the genotyping ability;while consistent results were obtained when comparing the kit with other commercially available *** addition,the whole PCR amplification can finish within approximately 45 min,making the system suitable for ***,only partial profiles may be obtained with challenging samples,including DNA stored on Foam-Tipped Applicators (FTA) cards or some case *** the forensic application in ethnic groups,a total of 282 and 229 alleles were obtained in Han and Mongolia,*** the 23 short tandem repeats were independent from each other,the cumulative power of exclusion in duos was 0.999999157188 and the cumulative power of exclusion in trios was 0.999999999859 in the Han group while the cumulative power of exclusion in duos (CPEduo) was 0.999 998 848 26 and cumulative power of exclusion in trios (CPEtrio) was 0.999 999 999 79 in the Mongolia *** good internal consistency was found between the two investigated groups and the Sichuan Han,Hui,Tibetan and Uygur according to available reference data.

关键词： Forensic genetics short tandem repeats(STRs) Early Access HuaxiaTM Platinum PCR kit

Wind regimes and associated sand dune types in the hinterland of the Badain Jaran Desert,China

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Journal of Arid Land 2022年第5期14卷 473-489页

作者： MENG Nan WANG Nai'ang zhao.Liqiang NIU zhenmin SUN Jiaqi Center for Glacier and Desert Research College of Earth and Environmental SciencesLanzhou UniversityLanzhou 730000China

Wind controls the formation and development of sand ***,understanding the wind regimes is necessary in sand dune *** this study,we combined the wind data from 2017 to 2019 at four meteorological stations(Cherigele and Wuertabulage stations in the lake basins,and Yikeri and Sumujilin stations on the top of sand dunes)in the hinterland of the Badain Jaran Desert in China,with high resolution Google Earth images to analyze the correlation between the wind energy environments and dune *** results of data analysis indicated that both the wind direction and sand drift intensity exhibited notable spatial and temporal *** highest level of wind activity was observed in *** and northeasterly winds were the dominant in the Badain Jaran *** the Cherigele,Wuertabulage,and Yikeri stations,the drift potential(DP)was below 200.00 vector units(VU).The wind energy environments in most areas could be classified as low-energy *** resultant drift direction differed at different stations and in different seasons,but the overall direction was mainly the *** resultant drift potential(RDP)/DP ratio was greater than 0.30 in most parts of the study area,suggesting that the wind regimes mainly exhibited unimodal or bimodal *** between the thermodynamic properties and the unique landscape settings of lakes and sand dunes could alter the local circulation and intensify the complexity of the wind *** wind regimes were weaker in the lake basins than on the top of sand *** dunes were the most dominant types of sand dunes in the study area,and the wind regimes at most stations were consistent with sand dune *** was thus the main dynamic factor affecting the formation of sand dunes in the Badain Jaran Desert *** results of this study are important for understanding the relationship between the wind regimes and aeolian landforms of the dune field in the desert

关键词： sand-driving wind drift potential wind energy environment sand dune local circulation Badain Jaran Desert

基于虚拟同步发电机的固态变压器交流端口柔性控制策略研究

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电力系统保护与控制 2023年第4期51卷 138-147页

作者：程静赵振民可再生能源发电与并网控制教育部工程研究中心(新疆大学) 新疆乌鲁木齐830017 国网新疆电力有限公司电力科学研究院新疆乌鲁木齐830011

固态变压器(solid state transformer, SST)能实现传统电力变压器的变压、隔离作用,还可实现灵活的电能调控作用,但不具备传统同步发电机的机械特性及同步运行机制。当分布式电源经SST接入电网时,由于其出力的随机性和波动性,将对上级... 详细信息

固态变压器(solid state transformer, SST)能实现传统电力变压器的变压、隔离作用,还可实现灵活的电能调控作用,但不具备传统同步发电机的机械特性及同步运行机制。当分布式电源经SST接入电网时,由于其出力的随机性和波动性,将对上级电网造成冲击,导致电网电压/频率波动。提出一种基于虚拟同步发电机的SST交流端口控制策略。首先,建立模块化多电平型输入级和同步发电机等效模型,将虚拟同步发电机原理融入输入级控制策略,以提高SST并网端口的电能质量、惯性及阻尼特性,并使其具备参与电网调频、调压能力。其次,提出储能装置辅助调频控制,保障SST参与一次调频时低压系统负载功率消耗平衡。最后,确立了输出级虚拟同步发电机控制策略,以提高低压交流端口负荷功率响应特性,实现SST对上级电网的友好性。在Matlab/Simulink平台搭建仿真模型,验证了所提SST拓扑结构及其控制策略的正确性和有效性。

关键词：虚拟同步发电机固态变压器分布式电源模块化多电平变换器控制策略

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Circular RNA circStag1 promotes bone regeneration by interacting with HuR

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Bone Research 2022年第3期10卷 565-577页

作者： Gaoyang Chen Canling Long Shang Wang zhenmin Wang Xin Chen Wanze Tang Xiaoqin He Zhiteng Bao Baoyu Tan Jin zhao.Yongheng Xie Zhizhong Li Dazhi Yang Guozhi Xiao Songlin Peng Department of Spine Surgery and Institute for Orthopaedic Research the Second Clinical Medical College of Jinan University(Shenzhen People’s Hospital)Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function RestorationShenzhen 518020China The First Affiliated Hospital Southern University of Science and TechnologyShenzhen 518055China The First Affiliated Hospital Jinan UniversityGuangzhou 510630China School of Medicine Southern University of Science and TechnologyGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchShenzhen Key Laboratory of Cell MicroenvironmentShenzhen 518055China

Postmenopausal osteoporosis is a common bone metabolic disorder characterized by deterioration of the bone microarchitecture,leading to an increased risk of ***,circular RNAs(circ RNAs)have been demonstrated to play pivotal roles in regulating bone ***,the underlying functions of circ RNAs in bone metabolism in postmenopausal osteoporosis remain ***,we report that circ Stag1 is a critical osteoporosis-related circ RNA that shows significantly downregulated expression in osteoporotic bone marrow mesenchymal stem cells(BMSCs)and clinical bone tissue samples from patients with *** of circ Stag1 significantly promoted the osteogenic capability of ***,we found that circ Stag1 interacts with human antigen R(Hu R),an RNA-binding protein,and promotes the translocation of Hu R into the cytoplasm.A high cytoplasmic level of Hu R led to the activation of the Wnt signaling pathway by stabilizing and enhancing low-density lipoprotein receptor-related protein 5/6(Lrp5/6)andβ-catenin expression,thereby stimulating the osteogenic differentiation of ***,overexpression of circ Stag1 in vivo by circ Stag1-loaded adeno-associated virus(circ Stag1-AAV)promoted new bone formation,thereby preventing bone loss in ovariectomized ***,we show that circ Stag1 plays a pivotal role in promoting the regeneration of bone tissue via Hu R/Wnt signaling,which may provide new strategies to prevent bone metabolic disorders such as postmenopausal osteoporosis.

关键词： metabolism thereby promoted

Application of decellularization-recellularization technique in plastic and reconstructive surgery

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Chinese Medical Journal 2023年第17期136卷 2017-2027页

作者： Yujia Shang Guanhuier Wang Yonghuan Zhen Na Liu Fangfei Nie zhenmin zhao.Hua Li Yang An Department of Plastic Surgery Peking University Third HospitalHaidian DistrictBeijing 100191China Wuya College of Innovation Key Laboratory of Structure-Based Drug Design&DiscoveryMinistry of EducationShenyang Pharmaceutical UniversityShenyangLiaoning 110016China

In the field of plastic and reconstructive surgery,the loss of organs or tissues caused by diseases or injuries has resulted in challenges,such as donor shortage and *** recent years,with the development of regenerative medicine,the decellularization-recellularization strategy seems to be a promising and attractive method to resolve these *** decellularized extracellular matrix contains no cells and genetic materials,while retaining the complex ultrastructure,and it can be used as a scaffold for cell seeding and subsequent transplantation,thereby promoting the regeneration of diseased or damaged tissues and *** review provided an overview of decellularization-recellularization technique,and mainly concentrated on the application of decellularization-recellularization technique in the field of plastic and reconstructive surgery,including the remodeling of skin,nose,ears,face,and ***,we proposed the challenges in and the direction of future development of decellularization-recellularization technique in plastic surgery.

关键词： Decellularization Recellularization Extracellular matrix Plastic surgery Tissue regeneration

Straight line feature based image distortion correction

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Engineering Sciences 2008年第2期6卷 83-86,96页

作者： Zhang Haofeng zhao.Chunxia Lu Jianfeng Tang zhenmin Yang Jingyu Dept. of Computer Science Nanjing Univ. of Sci. ＆ Tech. Nanjing Jiangsu 210094 China

An image distortion correction method is proposed, which uses the straight line features. Many parallel lines of different direction from different images were extracted, and then were used to optimize the distortion parameters by nonlinear least square. The thought of step by step was added when the optimization method working. 3D world coordination is not need to know, and the method is easy to implement. The experiment result shows its high accuracy.

关键词： distortion correction straight line feature nonlinear least square multi-step optimization

Identification of Estrogen-associated Intrinsic Aging Genes in Chinese Han Female Skin by cDNA Microarray Technology

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Biomedical and Environmental Sciences 2011年第4期24卷 364-373页

作者： YAN Wei zhao.zhenmin ZHANG LiLi WANG DunMei YAN Li YIN NingBei WU Di ZHANG Feng Plastic Surgery Hospital Chinese Academy of Medical Science Peking Union Medical College Beijing 100144 China CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100029 China

Objective Estrogens play an important role in intrinsic skin *** associated changes in global gene expression are poorly *** We used the Illumina microarray platform to obtain comprehensive gene expression profiles in female Chinese Han skin,and confirmed the data by quantitative real-time PCR （Q-RT-PCR）.Results We found 244 genes significantly related to estrogen-associated intrinsic skin aging,and some of these genes were confirmed by *** also performed functional analysis by both Gene Ontology annotation and enrichment of the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathways *** functional analysis revealed 11 biological pathways （including the KEGG pathways,the mitogen-activated protein kinase signaling pathway and metabolic pathways）,that were associated with multiple cellular functions which may be involved in intrinsic skin *** This study suggests that estrogen-associated intrinsic skin aging is a complicated biological process involving many genes and pathways.

关键词： Estrogen Intrinsic aging Microarray Metabolism

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不平衡电压条件下VSG-SST控制策略

太阳能学报 2023年第3期44卷 393-400页

作者：程静赵振民张陵南东亮新疆大学电气工程学院乌鲁木齐830017 新疆大学可再生能源发电与并网控制工程技术教育部工程研究中心乌鲁木齐830017 国网新疆电力有限公司电力科学研究院乌鲁木齐830011

当低压配电网电压不平衡时,产生的负序分量将引起传统VSG控制中SST输出级发生电流不平衡和功率振荡现象,为此该文提出基于改进VSG的T型三电平输出级联合控制策略,以提高输出级电能质量。首先,对不平衡电压下VSG电流不平衡及功率振荡进... 详细信息

当低压配电网电压不平衡时,产生的负序分量将引起传统VSG控制中SST输出级发生电流不平衡和功率振荡现象,为此该文提出基于改进VSG的T型三电平输出级联合控制策略,以提高输出级电能质量。首先,对不平衡电压下VSG电流不平衡及功率振荡进行机理分析;其次,基于VSG控制算法和瞬时功率理论设计新型电流基准发生器,与正负序电流调节器实现级联控制,以此保障SST输出电流平衡及有功/无功功率恒定,实现不同运行工况的可靠切换;最后,对正负序分量分离方法和中点电位平衡算法进行分析研究,并对控制策略进行仿真验证。结果表明,所提联合控制策略有效可行,能保障电压三相不平衡工况下SST输出级并网电流功率质量。

关键词：电能质量固态变压器虚拟同步机 T型三电平电网电压不平衡控制策略