检索结果-南通市图书馆

Trace determination of hexabromocyclododecane diastereomers in water samples with temperature controlled ionic liquid dispersive liquid phase microextraction

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2011年第1期22卷 97-100页

作者： Ru Song zhao xia wang Jin Peng Yuan Shan Shan wang Chuan Ge Cheng Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Analysis and Test Center Shandong Academy of Sciences Jinan 250014 China

A novel temperature controlled ionic liquid dispersive liquid phase microextracfion （TCIL-DLPME） coupled with rapid resolution liquid chromatography-electrospray tandem mass spectrometry （RRLC-ESI-MS-MS） has been developed for the enrichment and determination of three hexabromocyclododecane diastereomers （HBCDs） in water samples. Green solvent ionic liquid （IL） was used as extraction solvent instead of toxic organic solvents. This technique also avoided the usage of dispersive solvent. Some important parameters that might affect the extraction efficiency were optimized. Under the optimum conditions, good linear relationship, sensitivity and reproducibility were obtained. All the limits of detection for the three diastereomers were 0.1 ng/ mL. The linear range was obtained in the range of 1-100 ng/mL for the total amount of three HBCD diastereomers. It was satisfactory to analyze real environmental water samples with the recoveries ranging from 77.2% to 99.3%. The main advantage of the method is toxic organic solvent-free.

关键词： Hexabromocyclododecane Temperature controlled ionic liquid dispersive liquid phase microextraction Rapid resolution liquidchromatography-electrospray tandem mass spectrometry

The hazardous effects of tobacco smoking on male ertility

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2015年第6期17卷 954-960,I0009页

作者： Jing-Bo Dai zhao-xia wang Zhong-Dong Qiao School of Life Sciences and Biotechnology Shanghai Key Laboratory of Reproductive Medicine Shanghai Jiao Tong University Shanghai 200240 China

The substantial harmful effects of tobacco smoking on fertility and reproduction have become apparent but are not generally appreciated. Tobacco smoke contains more than 4000 kinds of constituents, including nicotine, tar, carbonic monoxide, polycyclic aromatic hydrocarbons, and heavy metals. Because of the complexity of tobacco smoke components, the toxicological mechanism is notably complicated. Most studies have reported reduced semen quality, reproductive hormone system dysfunction and impaired spermatogenesis, sperm maturation, and spermatozoa function in smokers compared with nonsmokers. Underlying these effects, elevated oxidative stress, DNA damage, and cell apoptosis may play important roles collaboratively in the overall effect of tobacco smoking on male fertility. In this review, we strive to focus on both the phenotype of and the molecular mechanism underlying these harmful effects, although current studies regarding the mechanism remain insufficient.

关键词： DNA fragmentation heavy metals nicotine oxidative stress semen quality spermatogenesis

T2 Mapping and Fat Quantification of Thigh Muscles in Children with Duchenne Muscular Dystrophy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2019年第1期39卷 138-145页

作者： Liang YIN Zhi-ying XIE Hai-yan XU Sui-sheng ZHENG zhao-xia wang Jiang-xi xiaO Yun YUAN Department of Radiology Peking University First HospitalBeijing 100034China Department of Neurology Peking University First HospitalBeijing 100034China Department of Radiology Second Affiliated Hospital of Anhui Medical UniversityHefei 230601China

Quantitative magnetic resonance image(MRI)in individual muscles may be useful for monitoring disease progression in Duchenne muscular dystrophy(DMD).The purpose of this study w批to measure丁2 relaxation time of thigh muscles in children with DMD and healthy boys,and to correlate the T2 relaxation time of muscles with the fat fraction(FF)at quantitative magnetic resonance and results of clinical ***-two boys with DMD and 18 healthy boys were evaluated with T2 mapping and three-point Dixon ***,body mass index(BMI),muscle strength assessment,timed functional tests(time to walk or run 10 metres,rise from the floor and ascend four stairs),and the North Star Ambulatory Assessment(NSAA)were ***’s correlation was used to assess the relationships between FF and clinical assessments and T2 relaxation *** mean T2 relaxation time of thigh muscles in DMD was significantly longer than that in the control group(P<0.05),except for the gracilis(P=0.952).The gracilis,sartorius and adductor longus were relatively spared by fatty infiltration in DMD *** T2 relaxation time was correlated significantly with the mean FF in all ***,BMI,total muscle strength score,timed functional tests and NSAA were significantly correlated with the overall mean T2 relaxation time.T2 mapping may prove clinically useful in monitoring muscle changes as a result of the disease process and in predicting the outcome of DMD patients.

关键词： T2 mapping Duchenne muscular dystrophy skeletal muscle fat infiltration

Diagnosis and Management of Kearns-Sayre Syndrome Rely on Comprehensive Clinical Evaluation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2016年第20期129卷 2519-2520页

作者： Meng Yu Lei Yu zhao-xia wang Department of Neurology Peking University First HospitalBeijing 100034China Department of Radiology Peking University First HospitalBeijing 100034China

We appreciate the readers＇ interest and their comprehensive comments and advice about the article,[1] which mainly concerned details of the patients＇ *** this paper,we reported 19 Kearns-Sayre syndrome （KSS） patients whose diagnoses were in accordance with the current clinical diagnostic criteria of KSS,i.e.,the triad of progressive external ophthalmoplegia,pigmentary retinopathy,and onset before 20 years of age,plus at least one of the followings：heart block,cerebellar symptoms,or cerebrospinal fluid protein levels above 1000 mg/L.[2] The diagnostic criteria have been widely used.[3] Apart from clinical features,muscle pathology and molecular genetic analysis canplay a great role in the diagnostic workup.

关键词：评价研究色素性视网膜炎心脏传导阻滞眼肌麻痹慢性进行性外侧综合征

Subretinal fibrosis secondary to neovascular age-related macular degeneration:mechanisms and potential therapeutic targets

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第2期20卷 378-393页

作者： Jingxiang Zhang xia Sheng Quanju Ding Yujun wang Jiwei zhao Jingfa Zhang Department of Ophthalmology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Urology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Ophthalmology Shanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghaiChina National Clinical Research Center for Eye Diseases Shanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineShanghai Engineering Center for Precise Diagnosis and Treatment of Eye DiseasesShanghaiChina

Subretinal fibrosis is the end-stage sequelae of neovascular age-related macular *** causes local damage to photoreceptors,retinal pigment epithelium,and choroidal vessels,which leads to permanent central vision loss of patients with neovascular age-related macular *** pathogenesis of subretinal fibrosis is complex,and the underlying mechanisms are largely ***,there are no effective treatment options.A thorough understanding of the pathogenesis of subretinal fibrosis and its related mechanisms is important to elucidate its complications and explore potential *** current article reviews several aspects of subretinal fibrosis,including the current understanding on the relationship between neovascular age-related macular degeneration and subretinal fibrosis;multimodal imaging techniques for subretinal fibrosis;animal models for studying subretinal fibrosis;cellular and non-cellular constituents of subretinal fibrosis;pathophysiological mechanisms involved in subretinal fibrosis,such as aging,infiltration of macrophages,different sources of mesenchymal transition to myofibroblast,and activation of complement system and immune cells;and several key molecules and signaling pathways participating in the pathogenesis of subretinal fibrosis,such as vascular endothelial growth factor,connective tissue growth factor,fibroblast growth factor 2,platelet-derived growth factor and platelet-derived growth factor receptor-β,transforming growth factor-βsignaling pathway,Wnt signaling pathway,and the axis of heat shock protein 70-Toll-like receptors 2/*** review will improve the understanding of the pathogenesis of subretinal fibrosis,allow the discovery of molecular targets,and explore potential treatments for the management of subretinal fibrosis.

关键词： choroidal neovascularization epithelial-mesenchymal transition mesenchymal transition myofibroblast neovascular age-related macular degeneration submacular fibrosis subretinal fibrosis therapeutic targets transforming growth factor-β vascular endothelial growth factor

Optical coherence tomography findings in chronic progressive external ophthalmoplegia

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2019年第10期132卷 1202-1207页

作者： Yuan Wu Lei Kang Hai-Long Wu Yue Hou zhao-xia wang Department of Ophthalmology Peking University First Hospital Beijing 100034 China Department of Neurology Peking University First Hospital Beijing 100034 China

Background: Chronic progressive external ophthalmoplegia (CPEO) is a mitochondrial encephalomyopathy caused by multiple mtDNA abnormalities. There is little information about the changes of ocular fundus with CPEO. The aim of this work was to measure and evaluate changes in the macular retinal thickness and optic nerve head in patients with CPEO using spectral-domain optical coherence tomography and to compare the findings with those of healthy individuals. Methods: Totally, 18 CPEO patients were enrolled in this study. Healthy volunteers matched for gender, age, and diopter settings were included as a control group. The retinal thickness of macular central fovea, inner and outer retinal layer thickness of perifoveal macular, optic nerve head parameters, and peripapillay retinal nerve fiber layer thickness (pRNFLT) for all included cases were measured using spectral-domain optical coherence tomography. A paired t test was used to compare the differences in the studied parameters between the two groups. The correlations between macular retinal thickness, pRNFLT, disease duration, and age of onset were also analyzed. Results: Among the macular parameters, retinal thickness of macular central fovea (t=—2.135, P < 0.05) and outer retinal layer thickness (t =—1.994, P < 0.05) of patients in the CPEO group were statistically significant lower than those of patients in the normal control group. For the optic nerve head parameters, the patients in the CPEO group showed a larger rim volume (t =—2.499, P < 0.05) and nerve head volume (t =—2.103, P < 0.05). The overall pRNFLT of patients in the CPEO group was statistically significant lower than that of patients in the control group (t =—4.125, P < 0.05). The comparison of pRNFLT in eight sectors showed that the pRNFLT of patients in the CPEO group was statistically significant lower than that of the control group mainly in the inferior and temporal sectors. The degree of pRNFL defect negatively correlated with the disease dur

关键词： Optical coherence tomography Chronic progressive external ophthalmoplegia Retina Nerve fiber layer

Extraction,Isolation and Structural Characterization of Polysaccharides from a Red Alga Gloiopeltis furcata

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2010年第2期9卷 193-197页

作者： YU Guangli HU Yannan YANG Bo zhao xia wang Peipei JI Guoli WU Jiandong GUAN Huashi Laboratory of Glycoscience and Glycoengineering School of Medicine and Pharmaeeutics Ocean University of China Qingdao 266003 P. R. China

Three kinds of polysaccharides： GFW, GFH and GFA, were sequentially extracted from a red alga Gloiopeltisfurcata with 25℃ and 85℃ water, and 60℃ 4% NaOH water solution. Based on the defatted alga, the yields of the polysaccharide were 57.9%, 2.5% and 2.6%, respectively. Their monosaccharide compositions, average molecular weights and structural characters were determined by gas chromatography （GC）, high performance liquid chromatography （HPLC）, fourier transform infrared spectroscopy （FTIR） or ^13C-NMR spectroscopy. The results showed that GFW, GFH and GFA were all composed of D-galactose （Gal） and 3,6-anhydro-L-galactose （AnG）, and particularly GFA also contained xylose （Xyl）. The average molecular weights of GFW, GFH and GFA were 22.6 kD, 26.5 kD and 49.8 kD, respectively, with the respective sulfate content 31.2%, 25.1% and 22.7%. The data of FTIR and ^13C-NMR confirmed the sulfate ester location at C6 ofgalactose. It is concluded that all the three polysaecharides extracted from Gloiopeltisfurcata were sulfated galactans, two being sulfated-agarose, and one being xylose-containing sulfated galactan.

关键词： Gloiopeltisfurcata sulfated galactan extraction structural characterization

Compound Heterozygote Mutation of C12orf65 Causes Distal Motor Neuropathy and Optic Atrophy

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2017年第2期130卷 242-244页

作者： xiao-Jing Fang Wei Zhang He Lyu zhao-xia wang Wei-Wei wang Yun Yuan Department of Neurology Peking University First Hospital Beijing 100034 China

The C12orf65 gene is a nuclear gene that encodes a mitochondrial matrix protein contributing to mitochondrial translation. C12orf65 gene-related diseases are rare and present with large heterophenotypes. Most of the reported patients have had optic atrophy with intellectual disability, encephalomyopathy, spastic paraplegia, and ophthalmoplegia. Peripheral neuropathy has been reported in one thmily. Here, we report a case of a Chinese patient with optic atrophy and distal motor neuropathy due to a novel compound heterozygous mutation in the C12orf65 gene.

关键词： C12orf65 Protein Inherited Peripheral Neuropathy Optic Atrophy

Levetiracetam administration is correlated with lower mortality in patients with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes: a retrospective study

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2019年第3期132卷 269-274页

作者： Zhe Zhang Dan-Hua zhao Xu-Tong zhao xiao Zhang Hui Xiong Xin-Hua Bao Yun Yuan zhao-xia wang Department of Neurology Peking University First HospitalBeijing 100034China Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Department of Neurology Peking University International HospitalBeijing 102206China Department of Neurology Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Pediatrics Peking University First HospitalBeijing 100034China

Background:Studies on the relationship between antiepileptic drug (AED) administration and clinical outcomes in patients with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) remain scarce. Levetiracetam (LEV) is an AED that is neuroprotective in various neurologic disorders. This study aimed to determine the impact of LEV on the outcome of ***:A retrospective, single-center study was performed based on a large cohort of patients with MELAS with a history of seizures (n=102). Decisions on antiepileptic therapies were made empirically. Patients were followed up for 1 to 8 years (median, 4 years) and divided into 2 groups based on whether LEV was administered (LEV or non-LEV). The modified Rankin scale (mRS) scores and mortality risks were analyzed in all ***:LEV, carbamazepine, benzodiazepines, topiramate, oxcarbazepine, valproate, and lamotrigine were administered in 48, 37, 18, 13, 11, 9, and 9 patients, singly or in combination, respectively. The mean mRS score of the LEV group (n=48) was lower than that of the non-LEV group (n=54;mean±standard deviation, 2.79±1.47 vs. 3.83±1.93, P=0.006) up to the end of the study. Nevertheless, there was no difference in the proportion of subjects without disability (mRS ranging 0-1) between the groups (P=0.37). The multivariate regressions revealed that LEV treatment was associated with lower mRS scores (odds ratio 0.32, 95% confidence interval [CI] 0.15-0.68, P=0.003) and mortality rates (hazard ratio 0.24, 95% CI 0.08-0.74, P=0.013). There was a significant difference in the Kaplan-Meier survival curves between the groups (χ^2=4.29, P=0.04).Conclusions:The LEV administration is associated with lower mortality in patients with MELAS in this retrospective study. Further laboratory research and prospective cohort studies are needed to confirm whether LEV has neuroprotective effects on patients with mitochondrial diseases.

关键词： Syndrome Seizures Epilepsy Anticonvulsants Levetiracetam Survival analysis