检索结果-南通市图书馆

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

奶牛卵泡颗粒细胞在卵泡发育中的作用机制

畜牧兽医学报 2024年第6期55卷 2313-2324页

作者：宋浩然冯肖艺张培培张航牛一凡余洲万鹏程崔凯赵学明中国农业科学院北京畜牧兽医研究所北京100193 青岛农业大学动物科技学院青岛266000 新疆农垦科学院畜牧兽医研究所石河子832000

随着奶牛产奶量的提高,我国奶业产业链也在不断优化和升级,朝着现代化、规范化和国际化的方向迈进。然而,高产奶牛的繁殖性能却在逐渐降低,目前,这已成为奶牛相关研究工作者面临的共同问题。卵泡发育与繁殖效率关系密切,卵泡正常发育是... 详细信息

随着奶牛产奶量的提高,我国奶业产业链也在不断优化和升级,朝着现代化、规范化和国际化的方向迈进。然而,高产奶牛的繁殖性能却在逐渐降低,目前,这已成为奶牛相关研究工作者面临的共同问题。卵泡发育与繁殖效率关系密切,卵泡正常发育是奶牛发情、排卵等繁殖活动的先决条件。其中,卵泡颗粒细胞能够与卵母细胞和卵泡膜细胞相互作用分泌多种激素和因子,同时,作为多种激素和细胞因子的受体影响卵泡发育。因此,本文总结了关于卵泡颗粒细胞功能与卵泡发育的相关研究,并以卵泡颗粒细胞在卵泡发育过程中的作用和颗粒细胞对卵母细胞的影响为重点进行综述,以期为今后相关研究提供理论参考。

关键词：颗粒细胞卵泡发育奶牛繁殖性能相互作用

同方期刊数据库博看期刊

A comparative analysis of China and other countries in metabolic engineering: Output, impact and collaboration

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2021年第2期34卷 37-45页

作者： Cong Chen Tao Chen Zhiwen Wang xueming zhao Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) SynBio Research PlatformCollaborative Innovation Center of Chemical Science and Engineering(Tianjin)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

In recent years,metabolic engineering has made great progress in both academic research and industrial ***,we have not found any articles that specifically analyze the current state of metabolic engineering in China in comparison with other ***,we review the current development and future trends of global metabolic engineering,conduct an in-depth benchmarking analysis of the development situation of China’s metabolic engineering,and identify current problems as well as future *** searched publications in the Scopus database from 2015 to September 2020 in the field of metabolic engineering,and analyzed the output in general,including publication trends,research distribution,popular journals,hot topics and vital institutions,but also analyzed the share of citations,field-weighted citation impact,and production in collaboration with strategic countries in science and *** study aims to serve as a reference for later studies,offering a comprehensive view of China’s contribution to metabolic engineering,and as a tool for the elaboration of national public policy in science and technology.

关键词： Metabolic engineering Output Citation impact Collaboration China Science metric

IGF1、CoQ10、MT联合添加缓解热应激对牛IVF囊胚的影响

在线全文

学校读者我要写书评

暂无评论

畜牧兽医学报 2024年第6期55卷 2474-2485页

作者：张航张培培杨柏高冯肖艺牛一凡余洲曹建华万鹏程赵学明中国农业科学院北京畜牧兽医研究所北京100193 新疆农垦科学院畜牧兽医研究所乌鲁木齐石河子832000

旨在探明胰岛素样生长因子1(insulin-like growth factors 1,IGF1)、辅酶Q10(coenzyme Q10,CoQ10)及褪黑素(melatonin, MT)联合添加对牛卵母细胞和胚胎发育以及热应激囊胚的影响。本研究于屠宰场采集牛离体卵巢,于实验室抽取卵丘卵母细... 详细信息

旨在探明胰岛素样生长因子1(insulin-like growth factors 1,IGF1)、辅酶Q10(coenzyme Q10,CoQ10)及褪黑素(melatonin, MT)联合添加对牛卵母细胞和胚胎发育以及热应激囊胚的影响。本研究于屠宰场采集牛离体卵巢,于实验室抽取卵丘卵母细胞复合体(cumulus-oocyte complexes, COCs),在牛卵母细胞体外成熟(in vitro maturation, IVM)液及牛胚胎体外培养(in vitro culture, IVC)液中添加IGF1、CoQ10及MT,检测各添加方式对牛卵母细胞发育能力、牛卵母细胞活性氧(reactive oxygen species, ROS)水平及线粒体膜电位(mitochondrial membrane potential,ΔΨm)的影响;在囊胚期施加41℃热应激,检测热应激及联合添加对牛IVF囊胚发育能力及凋亡水平的影响,并利用qRT-PCR检测囊胚中胚胎质量相关基因mRNA表达水平。各组试验均重复3次。结果表明,与未添加组(CT-0组)相比,联合添加IGF1、CoQ10及MT组(ICM组)卵母细胞成熟率((90.40±2.06)%vs.(65.41±0.63)%)、卵裂率((93.33±1.96)%vs.(59.77±2.93)%)及囊胚率((51.43±5.34)%vs.(26.92±3.24)%)均显著提高(P<0.05);ICM组牛卵母细胞ROS水平显著降低;ΔΨm显著提高(P<0.05)。与热应激组(HS组)相比,联合添加IGF1、CoQ10及MT(ICM+HS组)显著提高了囊胚扩张率((62.00±2.97)%vs.(30.77±8.66)%,P<0.05),抑制了热应激囊胚细胞凋亡,并提高了IGFBP3、ATP1A1、DSC2及IFNT2的mRNA表达水平(P<0.05)。综上表明,联合添加IGF1、CoQ10及MT有效提高牛卵母细胞发育能力,降低ROS水平,提高ΔΨm。热应激降低牛囊胚扩张率,促进牛囊胚细胞凋亡,影响囊胚质量,而联合添加IGF1、CoQ10及MT缓解了热应激损伤。

关键词： IGF1 CoQ10 褪黑素热应激奶牛胚胎

维普期刊数据库博看期刊

同方期刊数据库

不同单细胞全基因组扩增体系扩增牛微量血液DNA效果评价

在线全文

学校读者我要写书评

暂无评论

畜牧兽医学报 2024年第8期55卷 3436-3445页

作者：牛一凡李崇阳杨柏高张培培张航冯肖艺曹建华余洲马友记赵学明甘肃农业大学动物科学技术学院兰州730070 中国农业科学院北京畜牧兽医研究所北京100193

旨在通过二代测序技术评估不同单细胞全基因组扩增(single cell whole genome amplification,scWGA)体系对牛微量血液基因组DNA的扩增效果,建立微量DNA全基因组扩增体系。本研究分别采用MDA(multiple displacement amplification)和MALBAC(multiple annealing and looping-based amplification cycles)两种scWGA体系对华西牛1 ng血液基因组DNA进行全基因组扩增,随后基于两种体系的扩增产物以及原始未稀释血液DNA构建测序文库,利用DNBSEQ-T7RS测序平台进行全基因组测序(whole genome sequencing,WGS),通过比较扩增产物片段大小、浓度、总质量评估扩增效率,通过分析GC含量、测序覆盖度、基因分型一致率、基因型检出率等评估两种体系的扩增效果。结果显示,MDA体系的扩增产物片段大于MALBAC体系(8 kb vs.0.2~2 kb),产物浓度和总质量显著高于MALBAC体系(P<0.05)。基于测序数据,1×和5×测序深度下,MDA体系的基因组覆盖度显著高于MALBAC体系(P<0.05),此外,MDA体系的分型一致率、检出率显著高于MALBAC体系,而等位基因缺失率、假阳性率显著低于MALBAC体系(P<0.05)。综上,本研究揭示了MDA和MALBAC两种扩增体系基于华西牛1 ng血液基因组DNA扩增的体系特点,为改进现有华西牛胚胎基因组选择中的关键扩增技术提供理论依据,促进华西牛遗传育种进展。

关键词：全基因组选择扩增 MDA MALBAC 微量DNA扩增体系

Repurposing disulfiram with CuET nanocrystals:Enhancing anti-pyroptotic effect through NLRP3 inflammasome inhibition for treating inflammatory bowel diseases

维普期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第6期14卷 2698-2715页

作者： xueming Xu Yuanfeng Han Jiali Deng Shengfeng Wang Shijie Zhuo Kai zhao Wenhu Zhou Hematology and Department of Critical Care Medicine The Third Xiangya HospitalCentral South UniversityChangsha 410013China Xiangya School of Pharmaceutical Sciences Central South UniversityChangsha 410013China Hunan Chidren's Hospital Changsha 410007China Department of Pharmacy The Third Xiangya HospitalCentral South UniversityChangsha 410013China Key Laboratory of Biological Nanotechnology of National Health Commission Changsha 410008China

Drug repurposing offers a valuable strategy for identifying new therapeutic applications for existing ***,disulfiram(DSF),a drug primarily used for alcohol addiction treatment,has emerged as a potential treatment for inflammatory diseases by inhibiting pyroptosis,a form of programmed cell *** therapeutic activity of DSF can be further enhanced by the presence of Cu^(2+),although the underlying mechanism of this enhancement remains *** this study,we investigated the mechanistic basis of Cu^(2+)-induced enhancement and discovered that it is attributed to the formation of a novel copper ethylthiocarbamate(CuET)*** exhibited significantly stronger anti-pyroptotic activity compared to DSF and employed a distinct mechanism of ***,despite its potent activity,CuET suffered from poor solubility and limited permeability,as revealed by our druggability *** overcome these intrinsic limitations,we developed a scalable method to prepare CuET nanocrystals(CuET NCs)using a metal coordination-driven self-assembly *** studies demonstrated that CuET NCs exhibited a 6-fold improvement in ***,CuET NCs exhibited high biodistribution in the intestine,suggesting their potential application for the treatment of inflammatory bowel diseases(IBDs).To evaluate their therapeutic efficacy in vivo,we employed a murine model of DSS-induced colitis and observed that CuET NCs effectively attenuated inflammation and ameliorated colitis *** findings highlight the discovery of CuET as a potent anti-pyroptotic agent,and the development of CuET NCs represents a novel approach to enhance the druggability of CuET.

关键词： Drug repurposing Pyroptosis Nanoparticles Bioavailability Disulfiram DSS-induced colitis NLRP3 inflammasome CuET

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

能量负平衡影响奶牛卵泡发育的机制

畜牧兽医学报 2024年第1期55卷 22-30页

作者：曹建华杨柏高张培培冯肖艺张航余洲牛一凡郝海生杜卫华朱化彬杨凌赵学明中国农业科学院北京畜牧兽医研究所北京100193 河北工程大学生命科学与食品工程学院邯郸056038

奶牛能量负平衡(negative energy balance,NEB)是奶牛产后泌乳初期常会发生的一种代谢性疾病,当奶牛摄入能量无法满足其消耗能量时就会达到NEB状态。奶牛处于NEB状态时易引起患病率增加、妊娠率降低、生产性能下降,给集约化奶牛生产带... 详细信息

奶牛能量负平衡(negative energy balance,NEB)是奶牛产后泌乳初期常会发生的一种代谢性疾病,当奶牛摄入能量无法满足其消耗能量时就会达到NEB状态。奶牛处于NEB状态时易引起患病率增加、妊娠率降低、生产性能下降,给集约化奶牛生产带来很大的经济损失。随着分子、组学技术的广泛应用,奶牛泌乳初期NEB对牛卵泡发育的影响机制得到进一步揭示。本文根据近年来相关报道,综述奶牛早期泌乳NEB对牛卵泡发育分子机制相关研究进展并进行展望,以期为减少NEB对奶牛繁殖性能的不利影响提供理论参考。

关键词：能量负平衡奶牛泌乳期卵泡发育繁殖

Effects of NO_(2) and SO_(2) on the secondary organic aerosol formation fromβ-pinene photooxidation

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第2期136卷 151-160页

作者： Xiangyu Zang zhaoyan Zhang Yingqi zhao Gang Li Hua Xie Weiqing Zhang Guorong Wu xueming Yang Ling Jiang Zhang Dayu School of Chemistry Dalian University of TechnologyDalian 116024China State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry School of ScienceSouthern University of Science and TechnologyShenzhen 518055China

Elucidating the effects of anthropogenic pollutants on the photooxidation of biogenic volatile organic compounds is crucial to understanding the fundamental mechanisms of secondary organic aerosol(SOA)***,the impacts of NO_(2)and SO_(2)on SOA formation from the photooxidation of a representative monoterpene,β-pinene,were investigated by a number of laboratory *** results indicated NO_(2)enhanced the SOA mass concentrations and particle number concentrations under both low and highβ-pinene *** could be rationalized that the increased O_(3)concentrations upon the NO_(x)photolysis was helpful for the generation of more amounts of O_(3)-oxidized products,which accelerated the SOA nucleation and *** with NO_(2),the promotion of the SOA yield by SO_(2)was mainly reflected in the increase of mass concentration,which might be due to the elimination of the newly formed particles by the initially formed *** observed low oxidation degree of SOA might be attributed to the fast growth of SOA,resulting in the uptake of less oxygenated gas-phase species onto the particle *** present findings have important implications for SOA formation affected by anthropogenic–biogenic interactions in the ambient atmosphere.

关键词： Secondary organic aerosol Volatile organic compound Anthropogenic pollutant Beta-pinene Photooxidation

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2012年第28期57卷 3782-3789页

作者： DONG HuiNa zhao xueming MA YuanYuan ZHANG MinHua Tianjin University R&D Center for Petrochemical Technology Tianjin UniversityTianjin 300072China Department of Biochemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China State Key Laboratory of Engines Tianjin UniversityTianjin 300072China Key Laboratory of Systems Bioengineering Ministry of EducationTianjin UniversityTianjin 300072China Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology Tianjin UniversityTianjin 300072China

A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB *** of these components were determined to have significant effects on ethanol *** statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and *** validity of the developed model was *** ethanol concentration in the optimized medium was 6.7% higher than in the RM *** optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM *** and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant *** production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.

关键词：合成培养基乙醇生产优化重组发酵单胞菌木糖发酵响应曲面法中心复合设计柠檬酸三钠

Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Chemical Science and Engineering 2012年第2期6卷 174-178页

作者： Jixian GONG Nan DUAN xueming zhao School of Textiles Tianjin Polytechnic UniversityTianjin 300160China School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China

Evolutionary engineering is a novel whole- genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. Strain improvement ofPhaffia rhodozyma, one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of P. rhodozyma strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, P. rhodozyma AS2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of highproduction strain.

关键词： evolutionary engineering astaxanthin strain improvement