Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

作     者：DONG HuiNa ZHAO XueMing MA YuanYuan ZHANG MinHua 

作者机构：Tianjin University R&D Center for Petrochemical TechnologyTianjin UniversityTianjin 300072China Department of Biochemical EngineeringSchool of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China State Key Laboratory of EnginesTianjin UniversityTianjin 300072China Key Laboratory of Systems BioengineeringMinistry of EducationTianjin UniversityTianjin 300072China Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic BiologyTianjin UniversityTianjin 300072China 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2012年第57卷第28期

页      面：3782-3789页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the National Basic Research Program of China (2011CBA00804) the National Natural Science Foundation of China(30900033,20806055,20875068) Project of Introducing Talents of Discipline to Universities (B06006) 

主　　题：合成培养基 乙醇生产 优化重组 发酵单胞菌 木糖发酵 响应曲面法 中心复合设计 柠檬酸三钠 

摘      要：A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman(PB) design and response surface methodology(RSM).The effects of 19 medium components were investigated by PB *** of these components were determined to have significant effects on ethanol *** statistical model was constructed via central composite design(CCD) using five selected variables,including xylose,trisodium citrate,choline chloride,pyridoxine,and *** validity of the developed model was *** ethanol concentration in the optimized medium was 6.7% higher than in the RM *** optimized medium was then simplified to give the final synthetic medium(S2),in which the ethanol concentration was 20.7% higher than in the RM *** and trisodium citrate were the key medium components influencing ethanol production,while calcium pantothenate was the only growth factor required by recombinant *** production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.