检索结果-南通市图书馆

The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.

引用

Horticulture Research 2020年第1期7卷 1744-1757页

作者： xiting zhao Lingyu Song Liwei Jiang Yuting Zhu Qinghui Gao Dandan Wang Jing Xie Meng Lv Ping Liu Mingjun Li College of Life Sciences Henan Normal UniversityXinxiang 453007China Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province Xinxiang 453007China College of Mathematics and Information Science Henan Normal UniversityXinxiang 453007China

Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product ***,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria ***‘Huaiju 2#’plants were inoculated with Alternaria *** three or five *** analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the *** activity analysis also revealed a significant increase in defense enzyme activities in infected ***-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean *** these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria ***,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling *** particular,SA signaling was highly responsive to Alternaria *** qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq *** candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgen

关键词： sterile cultivar integration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua

引用

Molecular Plant 2019年第5期12卷 704-714页

作者： Rika Judd M.Caleb Bagley Mingzhuo Li Yue Zhu Caiyan Lei Seyit Yuzuak Mans Ekelof Gaobin Pu xiting zhao David C.Muddiman De-Yu Xie Department of Plant and Microbial Biology North Carolina State UniversityRaleighNCUSA Department of Chemistry North Carolina State UniversityRaleighNCUSA

Artemisinin-based combination therapy(ACT)forms the first line of malaria ***,the yield fluctuation of artemisinin has remained an unsolved problem in meeting the global demand for *** problem is mainly caused by the glandular trichome(GT)-specific biosynthesis of artemisinin in all currently used Artemisia annua ***,we report that non-GT cells of self-pollinated inbred *** plants can express the artemisinin biosynthetic *** expression analysis demonstrated the transcription of six known pathway genes in GT-free leaves and calli of inbred *** ***-qTOF-MS/MS analysis showed that these two types of GT-free materials produce artemisinin,artemisinic acid,and arteannuin *** IR-MALDESI image profiling revealed that these three metabolites and dihydroartemisinin are localized in non-GT cells of leaves of inbred *** ***,we employed all the above approaches to examine artemisinin biosynthesis in the reported XL annua glandless(gl)*** resulting data demonstrated that leaves of regenerated gl plantlets biosynthesize ***,these findings not only add new knowledge leading to a revision of the current dogma of artemisinin biosynthesis inannua but also may expedite innovation of novel metabolic engineering approaches for high and stable production of artemisinin in the future.

关键词： Artemisia annua artemisinin glandular trichome norvglandular trichome cell

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applications of cold atmospheric plasmas(CAPs)in agriculture:a brief review and the novel development of a radio-frequency CAP jet generator for plant mutation

引用

Plasma Science and Technology 2022年第9期24卷 1-14页

作者： Heping LI Jing LI xiting zhao Liyan WANG Chuan FANG Nan SU Chong ZHANG Yan XU Meng LV Mingjun LI Xinhui XING Department of Engineering Physics Tsinghua UniversityBeijing 100084People’s Republic of China College of Life Sciences Henan Normal UniversityXinxiang 453007People’s Republic of China Biobreeding Research Center Wuxi Research Institute of Applied TechnologiesTsinghua UniversityWuxi 214072People’s Republic of China MOE Key Laboratory of Industrial Biocatalysis Department of Chemical EngineeringTsinghua UniversityBeijing 100084People’s Republic of China Center for Systems and Synthetic Biology Tsinghua UniversityBeijing 100084People’s Republic of China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055People’s Republic of China

Cold atmospheric plasmas(CAPs)have shown great applicability in *** kinds of CAP sources have been studied in agricultural applications to promote plant growth and cure plant *** briefly review the state-of-the-art stimulating effects of atmospheric-pressure dielectricbarrier-discharge(AP-DBD)plasmas,after the direct or indirect treatment of plants for growth promotion and disease *** then discuss the special demands on the characteristics of the CAP sources for their applications in plant mutation *** atmospheric and room temperature plasma(ARTP)jet generator with a large plasma irradiation area,a high enough concentration of chemically reactive species and a low gas temperature is designed for direct plant *** measurements of the electrical,thermal and optical features of the ARTP generator are ***,an ARTP-P(ARTP for plant mutagenesis)mutation breeding machine is developed,and a typical case of plant mutation breeding by the ARTP-P mutation machine is presented using Coreopsis tinctoria *** and agricultural experiments show that the newly-developed ARTP-P mutation breeding machine with a large irradiation area can generate uniform CAP jets with high concentrations of chemically reactive species and mild gas temperatures,and have signiflcant mutagenesis effects on the Coreopsis tinctoria *** ARTP-P mutation breeding machine may provide a platform for systematic studies on mutation mechanisms and results for various plant seeds under different operating conditions in future research.

关键词： cold atmospheric plasma radio-frequency glow discharge mutation plant breeding ARTP agricultural application

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Activity Gel Analysis of Endopeptidases in Rose Petals

引用

Horticultural Plant Journal 2016年第2期2卷 114-120页

作者： zhao xiting ZHANG Changqing ZHU Yuting LI Tianzhong GAO Junping College of Life Sciences Henan Normal University Department of Ornamental Horticulture and Landscape China Agricultural University Department of Pomology China Agricultural University

Poor resolution for the identification of endopeptidase(EP) activity in activity gel assays is a critical issue in the analysis of the postharvest physiology of rose petals. In this paper, major factors influencing EP activity gel assays were evaluated. The results showed that a phosphate(NaH_2PO_4/Na_2HPO_4) buffer favors the detection of clear EP bands, as compared to Tris–HCl buffer. Removal of salts and pigments with Sephadex G-25 columns was vital to the measurement of EP activity in rose petal extracts. For optimal resolution of bands, we show that before electrophoresis,samples should be treated for 10 min at 40 °C. Additionally, electrophoresis should be done in 12% SDS–PAGE co-polymerized with 0.15%(w/v) gelatin. After electrophoresis, the optimal incubation temperature and pH are 42 °C and 7.0, respectively. Using our optimized assay, Rh-EP1,Rh-EP2, Rh-EP3, three proteases with molecular masses of 200, 123.5, and 97.4 kD, respectively, were detected in rose petals. Experiments using EP class-specific inhibitors revealed that Rh-EP2 and Rh-EP3 were both serine proteases, while Rh-EP1 was a metalloprotease. In this study, we also measured changes in EP activity during flower opening, senescence, and water deficit stress(WDS) using our optimized activity gel assay,and found that Rh-EP3 may be more relevant to senescence in roses compared with Rh-EP1 or Rh-EP2. Changes occurring to EPs after WDS were similar to those during the period from flower opening to senescence, and Rh-EP3 activities were greatly increased by WDS treatment. Collectively, our results suggest that significant increases in Rh-EPs activities, especially increases in Rh-EP3 activity, may contribute to the flower senescence induced under WDS treatment.

关键词： rose petal endopeptidase activity gel

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes of Relevant Indexes of Photosynthetic Pigment and Nitrogen Metabolism in Leaves of Chrysanthemum morifolium Ramat. ‘Huaibai' after TAV-free

引用

Plant Diseases and Pests 2016年第4期7卷 6-9,15页

作者： zhao xiting Liu Ke Zhu Yuting Wang zhaoyang Zhou Yingyuan Fan Zijian Jiang Liwei Li Mingjun College of Life Science Henan Normal University Xinxiang 453007 China Henan Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs Xinxiang 453007 China Henan Engineering Laboratory of Green Crude Drugs Bioteehnology Xinxiang 453007 China Nanzhao Development Office of Chinese Crude Drugs Nanyang 474650 China

[ Objective ] The paper was to explore effects of Tomato aspermy virus （TAV） -free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. ＇Huaibai＇, in order to provide a theoretical basis for field popularization and application of TAV-free ＇ Huai bai＇ plantlets. [ Method] With previously acquired TAV-free test-tube plantlets of ＇ Huaibai＇ as the experimental materials and its ordinary test-tube plantlets as the control （CK）, chlorophyll content and some indexes related to nitrogen metabolism in leaves were determined during subculture. [ Result] During subculture, the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free ＇ Huaibai＇ test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22＇h day of subculture, and their increase amplitudes were 5.24% and 41.69%, respectively. Compared with CK, the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subculture, with significant difference （P 〈0.05） or extremely significant difference （P 〈0.01 ）. Further- more, the nitrate reductase （NR） activity, free proline and soluble protein contents of TAV-free ＇ Huaibai＇ were all significantly higher than those of CK, and the highest increase amplitudes were 27.99% （ subculture for 22 d）, 28.10% （ subculture for 22 d） and 5.89% （ subculture for 38 d）, respectively. [ Conclusion] TAV-free could effectively improve chlorophyll content and nitrogen metabolism level of ＇ Huaibai＇ , and was conducive to its growth and development.

关键词： Chrysanthemum murifolium Ramat. ' Huaibai' Tomato aspermy virus Virus-free Chlorophyll content Nitrogen metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

审美概念理解对于创造性思维作业成绩的影响

引用

心理科学 2002年第6期25卷 649-652页

作者：赵伶俐黄希庭西南师范大学心理系重庆400715

本研究以小学 3、5年级、初 2、高 2、大 2年级学生共 30 0人为被试 (实验组 15 6人 ;对照组 15 4人 ) ,对审美概念实验教学前后测验结果分析表明 :审美概念理解程度对于创造性思维作业成绩有显著影响 ,各年级都存在这种效果 ;总的看女... 详细信息

本研究以小学 3、5年级、初 2、高 2、大 2年级学生共 30 0人为被试 (实验组 15 6人 ;对照组 15 4人 ) ,对审美概念实验教学前后测验结果分析表明 :审美概念理解程度对于创造性思维作业成绩有显著影响 ,各年级都存在这种效果 ;总的看女生显著高于男生。审美概念理解的 7个因子 (概念理解流畅、释义正确、释义精确、事实迁移长度、事实迁移宽度、类词迁移长度、类词迁移宽度等 )与创造性思维的两类作业 (图形、语言 )、6个因子 (流畅、独创、精致、灵活、标题抽象、抗封闭 )之间均存在显著相关 ;其中“类词迁移宽度”、“释义精确”、“类词迁移长度”等三个因素对创造性思维作业总成绩影响显著。

关键词：审美概念理解创造性思维作业成绩类词迁移宽度释义精确度回归分析类词迁移长度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

无焦压缩光路系统的计算机辅助装调方法

引用

应用光学 2016年第4期37卷 607-611页

作者：岳鹏远赵希婷赵振万玉曦北京空间机电研究所北京100094

为了解决随机成分多、调节灵敏度高的离轴反射式系统的精确装调问题,介绍了干涉测量与计算机优化技术相结合的方法,建立了各元件失调量与初级像差之间的关系,以及元件间的像差相互补偿关系,通过干涉测量采集系统全视场的波前差,反向优... 详细信息

为了解决随机成分多、调节灵敏度高的离轴反射式系统的精确装调问题,介绍了干涉测量与计算机优化技术相结合的方法,建立了各元件失调量与初级像差之间的关系,以及元件间的像差相互补偿关系,通过干涉测量采集系统全视场的波前差,反向优化得到各元件的失调量,在波长为632.8nm时,无焦压缩光路系统全视场平均出射波前优于RMS0.055λ,压缩比误差小于5‰,实现了无焦压缩光路系统高精度、高效率的装调。

关键词：遥感计算机辅助装调无焦压缩光路系统灵敏度矩阵

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

测定种子生活力方法的比较与改进

引用

实验技术与管理 2011年第1期28卷 46-48页

作者：刘亚丽赵喜亭河南师范大学生命科学学院河南新乡453007

用氯化三苯基四氮唑(TTC)法、红墨水法和溴麝香草酚蓝(BTB)法测定种子生活力,对实验选用的种子、浸泡时间、TTC的药液配置浓度、红墨水染色法染色时间、BTB法加入琼脂浓度及琼脂凝胶层厚度作了改进。结果表明:缩短了3种方法测定种子生... 详细信息

用氯化三苯基四氮唑(TTC)法、红墨水法和溴麝香草酚蓝(BTB)法测定种子生活力,对实验选用的种子、浸泡时间、TTC的药液配置浓度、红墨水染色法染色时间、BTB法加入琼脂浓度及琼脂凝胶层厚度作了改进。结果表明:缩短了3种方法测定种子生活力的时间,且效果更好,测定的准确率更高;TTC染色法红色更深,红墨水染色法种胚与胚乳更易分辨。

关键词：种子生活力氯化三苯基四氮唑法红墨水法溴麝香草酚蓝法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

最短路径算法在矿山巷道三维模型网络分析中的应用

引用

金属矿山 2015年第4期44卷 273-277页

作者：车德福陈军伟赵西亭东北大学资源与土木工程学院辽宁沈阳110819

矿山巷道三维模型能真实地模拟井下的工作场景,基于该模型的网络分析对煤矿井下安全救援十分重要。根据巷道的网络特点,将实际的测量数据中点状和线状元素抽象为节点-弧段图,该图的生成对应着一维中心线和二维双线巷道的构建,在此基础... 详细信息

矿山巷道三维模型能真实地模拟井下的工作场景,基于该模型的网络分析对煤矿井下安全救援十分重要。根据巷道的网络特点,将实际的测量数据中点状和线状元素抽象为节点-弧段图,该图的生成对应着一维中心线和二维双线巷道的构建,在此基础上根据断面的拱高、墙高及拓扑关系进行井巷模型基本单元自动的装配以及三角化生成巷道的三维模型。网络分析采用能适应拓扑变化的Dijkstra算法,从减少搜索节点和采用邻接表的存储结构两方面对传统的Dijkstra算法进行优化,并分析了算法的效率。最后编写程序实现了改进后算法在巷道三维模型中存在障碍的情况下的最短路径分析,并能在三维巷道中漫游显示,结果表明该算法快捷有效。

关键词：最短路径算法 Dijkstra 三维模型巷道网络漫游

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

多光谱相机计算机辅助装调方法

引用

光学学报 2015年第8期35卷 152-158页

作者：张超魏学敏赵希婷焦文春廖志波北京空间机电研究所北京100094

应用于航天遥感领域的光学相机光学元件多,分离变量多,结构复杂,成像质量要求接近衍射极限,对光学装调有着苛刻的要求。结合装调数学模型,将CODE V光学设计软件与各视场干涉图的Zernike系数整合为装调变量,优化求取失调量;根据离轴三反... 详细信息

应用于航天遥感领域的光学相机光学元件多,分离变量多,结构复杂,成像质量要求接近衍射极限,对光学装调有着苛刻的要求。结合装调数学模型,将CODE V光学设计软件与各视场干涉图的Zernike系数整合为装调变量,优化求取失调量;根据离轴三反系统理想光学模型求取装调灵敏度矩阵及确定多标量修正次序,有效克服装调变量相关性。在波长λ为632.8 nm时,成功将多光谱相机装调结果均方根(RMS)由0.563λ提升至0.064λ,实现了多光谱相机高精度、高效率的装调。

关键词：遥感计算机辅助装调反向优化多光谱相机

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：