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Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew

Phyton-International Journal of Experimental Botany 2023年第11期92卷 3043-3061页

作者： Lanjun Li degang zhao Key Laboratory of Mountain Plant Resources Protection and Germplasm Innovation of the Ministry of Education College of Life Sciences/Institute of Agricultural BioengineeringGuizhou UniversityGuiyang550025China Plant Conservation Technology Center Guizhou Key Laboratory of Agricultural BiotechnologyBiotechnology Institute of GuizhouGuizhou Academy of Agricultural SciencesGuiyang550006China

Various strains of powdery mildew(PM),a notorious plant fungal disease,are prevalent and pose a significant threat to plant *** control PM,transgenic technology can be used to cultivate more resistant plant *** the present study,we utilized the rapid amplification of cDNA ends(RACE)technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance *** analysis revealed that this gene belongs to the GH18 family and is classified as a class III *** EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant,with the most abundant expression in male *** localization analysis indicated that the protein encoded by this gene was detected within both the cell membrane and *** PM inoculation,overexpression of EuCHIT30.7 in tobacco plants led to a significantly reduced relative lesion area and a decreased spore count compared to both wild-type and empty vector control *** of the protective enzymes,namely,peroxidase(POD),superoxide dismutase(SOD),catalase(CAT),and phenylalaninammo-nialyase(PAL),in tobacco plants overexpressing EuCHIT30.7 were significantly greater than those in wild-type and empty vector tobacco ***,the rate of increase in malondialdehyde(MDA)content was significantly lower in tobacco plants expressing EuCHIT30.7 compared to control tobacco *** EuCHIT30.7 transgenic tobacco,the expression of pathogen-related protein genes,namely,PR2,PR5,PR1a,PDF1.2,and MLP423,along with the tobacco PM negative regulatory gene,MLO2,were significantly higher compared to control tobacco *** findings suggested that EuCHIT30.7 significantly enhances the resistance of tobacco to PM.

关键词： Eucommia ulmoides Oliv. EuCHIT30.7 powdery mildew chitinase tobacco

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Simulation of polarization effects in AlGaN/GaN heterojunction

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Science China(Physics,Mechanics & Astronomy) 2004年第6期47卷 694-701页

作者： LI Na zhao degang YANG Hui State Key Laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China State Key Laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China State Key Laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China

A method for introducing polarization effects in the simulation of GaN-based heterojunction devices is proposed. A δ doping layer is inserted at the interface of heterojunction and the ionized donors or acceptors act as polarization induced fixed charges. Thus polarization effects can be taken into account in a traditional device simulator. Ga-face and N-face single AlGaN/GaN heterostructures are simulated, and the simulation results show that carrier confinement takes place only in the former structure while not in the latter one. The sheet density of free electrons at the interface of Ga-face AlGaN/GaN increases with the Al composition and the thickness of AlGaN. The consistence of simulation results with the experiments and calculations reported elsewhere shows that this method can effectively introduce polarization effects in the simulation of GaN-based heterojunction devices.

关键词： AlGaN/GaN, heterojunction, polarization, simulation.

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Analysis of the growth of GaN epitaxy on silicon

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Journal of Semiconductors 2018年第3期39卷 31-33页

作者： Danmei zhao degang zhao Department of Electronic Information and Physics Changzhi University State Key Laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Science

Due to the great potential of Ga N based devices, the analysis of the growth of crack-free Ga N with high quality has always been a research hotspot. In this paper, two methods for improving the property of the Ga N epitaxial layer on Si（111） substrate are researched. Sample A, as a reference, only has an Al N buffer between the Si substrate and the epitaxy. In the following two samples, a Ga N transition layer（sample B） and an Al Ga N buffer（sample C） are grown on the Al N buffer separately. Both methods improve the quality of Ga N. Meanwhile, using the second method, the residual tensile thermal stress decreases. To further study the impact of the two introduced layers, we investigate the stress condition of Ga N epitaxial layer by Raman spectrum. According to the Raman spectrum, the calculated residual stress in the Ga N epitaxial layer is approximately 0.72 GPa for sample B and0.42 GPa for sample C. The photoluminescence property of Ga N epitaxy is also investigated by room temperature PL spectrum.

关键词： GaN stress MOCVD

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Fast Multipole Accelerated Boundary Integral Equation Method for Evaluating the Stress Field Associated with Dislocations in a Finite Medium

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Communications in Computational Physics 2012年第6期12卷 226-246页

作者： degang zhao Jingfang Huang Yang Xiang Nano Science and Technology Program The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong School of Physics HuazhongUniversity of Science and TechnologyWuhan 430074China Department ofMathematics The University of North Carolina at Chapel HillChapel HillNC 27599-3250USA Department of Mathematics The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong

In this paper,we develop an efficient numericalmethod based on the boundary integral equation formulation and new version of fast multipole method to solve the boundary value problem for the stress field associated with dislocations in a finite *** examples are presented to examine the influence from material boundaries on dislocations.

关键词： Fast multipole method boundary integral equation method dislocation dynamics stress

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Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1377-1389页

作者： Jiali Wang Xiangmei Chen Xiaozhen Huang Yichen zhao degang zhao The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Tea SciencesCollege of Life SciencesGuizhou UniversityGuiyang550000China Plant Conservation Technology Center Guizhou Key Laboratory of Agricultural BiotechnologyGuizhou Academy of Agricultural SciencesGuiyang550000China

The phytoene desaturase(PDS)encodes a crucial enzyme in the carotenoid biosynthesis *** or inhibiting PDS expression leads to the appearance of mottled,chlorosis,or albino *** this study,the CDS sequence of EuPDS(Eucommia ulmoides Phytoene Desaturase)was first cloned and then PDS was silenced in Nicotiana *** showed the expression level of EuPDS in leaves was higher than that in the roots and *** *** leaves,which were treated by Agrobacterium for 24 h,photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the period of *** suggested that EuPDS could silence PDS of ***,so as to cause the phenotype of leaf *** is the main reporter gene involved in virus-induced gene silencing(VIGS).This study offered molecular evidence for identifying PDS gene involved in Carotenoid’s biosynthesis pathway and the regulation networks in *** also laid a useful foundation for study on leaf discoloration mechanism of other woody plants.

关键词： Eucommia ulmoides EuPDS virus-induced gene silencing TRV relative expression

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Isolation of a novel antifungal peptide from the bark of Eucommia ulmoides Oliv

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High Technology Letters 2008年第2期14卷 216-219页

作者：刘世会 zhao degang Han Yuzhen Guizhou Key Laboratory of Agricultural Bioengineering College of Life Sciences Guizhou University Guiyang 550025 P.R. China Key Laboratory of Green Pesticide and Agricultural Biological Engineering Ministry of Education Guizhou University Guiyang 550025 P.R. China College of Biological Sciences China Agricultural University Beijing 100094 P.R. ChinaI

A novel Eucommia antifungal peptide, named EAFP3, was isolated from the bark of Eucommia ul- moides by NaC1 extract, gel filtration and reverse phase high performance liquid chromatography. The molecular mass of EAFP3 is 4157.3 Da, and its partial amino acid sequence is -. LYQQLIAGITLNK.-. EAFP3 exerts an inhibitory activity against Candida albicans in vitro and the drug concentration required for 50% growth inhibition （IC50） is 31.25μg/mL.

关键词： antifungal peptide isolation the bark of Eucommia ulmoides Oliv Candida albicans

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Overexpression ofβ-1,4-Glucanase Gene EuEG1 Improves Micrografting of Eucommia ulmoides

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Phyton-International Journal of Experimental Botany 2023年第11期92卷 3063-3075页

作者： Lei Wang Runying Wang Yi Li Yichen zhao degang zhao The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Tea SciencesCollege of Life SciencesGuizhou UniversityGuiyang550025China Guizhou Plant Conservation Technology Center Guizhou Academy of Agricultural SciencesGuiyang550006China Department of Plant Science and Landscape Architecture University of ConnecticutStorrsConnecticutUSA

Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of Eucommia *** offers a new method for the transplantation of genetic transformation,and its success depends on the formation of graft *** study used transgenic rootless test-tube seedlings as scions and seedlings from seed as rootstocks during micrografting to avoid the rooting issues that occur during tissue culture and to investigate the role of the EuEG1 gene in the graft healing *** found that the EuEG1 gene is a vital regulator of graft,and its overexpression contributes to the survival of Eucommia ulmoides *** EuEG1 gene transgenic plants(TP)used as scions for micrografting presented a significantly higher survival rate than the wild type(WT)and empty vector(EV)regenerated *** the grafting healing process,the expression of the EuEG1 gene was higher during the period of callus proliferation,suggesting that the EuEG1 gene was involved in the graft healing *** observation revealed that more calluses tissue appeared at the junction of transgenic scions,and the connection with the rootstock was stronger,which benefits wound *** results provide new insights into Eucommia ulmoides micrografting and indicate that the EuEG1 gene can promote wound healing and improve the micrografting survival rate.

关键词： Eucommia ulmoides genetic transformation EuEG1 gene micrografting

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A study on the minority carrier diffusion length in n-type GaN films

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Rare Metals 2007年第3期26卷 271-275页

作者： DENG Dongmei zhao degang WANG Jinyan YANG Hui WEN Cheng Paul Institute of Microelectronics Peking University Beijing 100871 China State Key Laboratory on Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China

The minority carrier diffusion length of n-type GaN fdms grown by metalorganic chemical vapor deposition （MOCVD） has been studied by measuring the surface photovoltaic （PV） spectra. It was found that the minority carrier diffusion length of undoped n-type GaN is considerably larger than that in lightly Si-doped GaN. However, the data suggested that the dislocation and electron concentration appear not to be responsible for the minority cartier diffusion length. It is suggested that Si doping plays an important role in decreasing the minority carrier diffusion length.

关键词： compound semiconductor material minority carrier diffusion length photovoltaic spectrum GaN

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Ⅲ-nitride based ultraviolet laser diodes

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Journal of Semiconductors 2019年第12期40卷 9-10页

作者： degang zhao State Key Laboratory of Integrated Optoelectronics Institute of Semiconductors Chinese Academy of Sciences Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences

In recent years,because of their small size,high efficiency and environment-friendly advantages,Ⅲ-nitride based ultraviolet(UV)light-emitting diodes(LEDs)have been widely used in many areas to substitute for mercury lamps,such as in 3D printing,curing and sterilization.Ⅲ-nitride alloys cover the whole UV spectrum which is comprised of UV-A(320–400 nm),UV-B(280–320 nm)and UV-C(200–280 nm)by controlling Al/Ga/In *** addition,Ⅲ-nitride based UV laser diodes(LDs)also have some potential applications in the case of high-power-density,narrow-spectrum,good-directional ***,Ⅲ-nitride based UV laser diodes still have many challenges such as poor crystal quality and low hole concentration in p-type AlGaN.

关键词： diodes laser ultraviolet

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Genome-level diversification of eight ancient tea populations in the Guizhou and Yunnan regions identifies candidate genes for core agronomic traits

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Horticulture Research 2021年第1期8卷 2559-2568页

作者： Litang Lu Hufang Chen Xiaojing Wang Yichen zhao Xinzhuan Yao Biao Xiong Yanli Deng degang zhao College of Tea Science Guizhou UniversityGuiyang 550025People’s Republic of China College of Life Sciences and The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in the Mountainous Region(Ministry of Education) Institute of Agro-BioengineeringGuizhou UniversityGuiyang 550025People’s Republic of China Guizhou Academy of Agricultural Sciences Guiyang 550025People’s Republic of China

The ancient tea plant,as a precious natural resource and source of tea plant genetic diversity,is of great value for studying the evolutionary mechanism,diversification,and domestication of *** overall genetic diversity among ancient tea plants and the genetic changes that occurred during natural selection remain poorly ***,we report the genome resequencing of eight different groups consisting of 120 ancient tea plants:six groups from Guizhou Province and two groups from Yunnan *** on the 8,082,370 identified high-quality SNPs,we constructed phylogenetic relationships,assessed population structure,and performed genome-wide association studies(GWAS).Our phylogenetic analysis showed that the 120 ancient tea plants were mainly clustered into three groups and five single branches,which is consistent with the results of principal component analysis(PCA).Ancient tea plants were further divided into seven subpopulations based on genetic structure ***,it was found that the variation in ancient tea plants was not reduced by pressure from the external natural environment or artificial breeding(nonsynonymous/synonymous=1.05).By integrating GWAS,selection signals,and gene function prediction,four candidate genes were significantly associated with three leaf traits,and two candidate genes were significantly associated with plant *** candidate genes can be used for further functional characterization and genetic improvement of tea plants.

关键词： Guizhou ancient integrating

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