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Biosynthesis, property comparison, and hemocompatibility of bacterial and haloarchaeal poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

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Science Bulletin 2015年第22期60卷 1901-1910页

作者： youxi zhao Zhiming Rao Yanfen Xue Ping Gong Yizhi Ji Yanhe Ma The Key Lab of Industrial Biotechnology Ministry of EducationSchool of Biotechnology Jiangnan University Biochemical Engineering College Beijing Union University State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences

Polyhydroxyalkanoates （PHAs） are a class of natural biopolyesters accumulated intracellularly by many microorganisms. These polymers have attracted particular attention as green plastic in biomedical and industrial applications due to their good biodegradability and bio- compatibility. Poly（3-hydroxybutyrate-co-3-hydroxyvaler- ate）（PHBV） is one of the most common members of PHAs. However, there is no report comparing the properties of PHBV from different groups of producers, e.g., bacteria and haloarchaea. In this study, two types of PHBV copolymers were synthesized in Halogranum amylolyticum and Ralstonia eutropha, respectively, by feeding different carbon sources. They possessed a similar concentration of 3HV monomers （21 tool%） and were named PHBV-H （produced by H. arnylolyticum） and PHBV-B （produced by R. eutropha） based on their source. Interestingly, they exhibited different behaviors especially in thermal stabil- ity, melting temperature, crystallinity percentage, and mechanical properties. Furthermore, the films of PHBV-Hand PHBV-B possessed different surface properties, such as surface roughness, wettability, and surface free energy. The value of hemolysis on the PHBV-H film was lower in comparison with the PHBV-B film, although both values were within the limit of 5 % permissible for biomaterials. Notably, few inactivated platelets adhered to the surface of the PHBV-H film, whereas numerous activated platelets were seen on film PHBV-B. These results indicated that PHBV-H was a better potential component of blood-contact biomaterials than PHBV-B. Our study clearly revealed that the properties of PHAs are source dependent and haloarchaeal species provide a new opportunity for the production of desired PHAs.

关键词： Polyhydroxyalkanoates Haloarchaealbiopolymer Microbial synthesis Wettability Hemocompatibility

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High level expression of Saccharomyces cerevisiae chitinase(ScCTS1)in Pichia pastoris for degrading chitin

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International Journal of Agricultural and Biological Engineering 2015年第5期8卷 142-150页

作者： zhao youxi Jiang Huihui Rao Zhiming Ji Yizhi Cheng Yanling Ma Yanhe Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Beijing Key Laboratory of Biomass Waste Resource Utilization Biochemical Engineering CollegeBeijing Union UniversityBeijing 100023China State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Chitin is the second most abundant renewable biopolymer in the *** play important roles in the degradation of *** are produced by different organisms for different purposes,which are widely expressed in the three domains of life,ranging from archaea,bacteria,to fungi,yeasts,plants,insects,and even *** there are few reports about Saccharomyces cerevisiae chitinase(ScCTS1).The aim of this study was to realize the high level expression of *** ScCTS1 was cloned into the expression vector *** recombinant plasmid was linearized and transformed into competent Pichia pastoris *** screening by G418 plate,the fermentation conditions were ***,under the optimal fermentation conditions,ScCTS1 enzymatic activity reached up to 94.6 U/*** paper presents the first report on the heterologous expression of a full-length ScCTS1 with considerably high *** work will not only make a great stride towards its potential applications in biotechnology,but also facilitate elucidating the precise mechanism of yeast cell division.

关键词： chitinases heterologous expression Saccharomyces cerevisiae pichia pastoris biotechnology

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Recycling arsenic-containing bio-leaching residue after thermal treatment in cemented paste backfill:Structure modification,binder properties and environmental assessment

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International Journal of Minerals,Metallurgy and Materials 2024年第10期31卷 2136-2147页

作者： Dengfeng zhao Shiyu Zhang Yingliang zhao Department of Mining Engineering Lyuliang UniversityLvliang 033000China School of Civil and Environmental Engineering University of Science and Technology BeijingBeijing 100083China School of Mining Engineering Taiyuan University of TechnologyTaiyuan 030024China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong Kong 00852China

The substantial arsenic(As)content present in arsenic-containing bio-leaching residue(ABR)presents noteworthy environ-mental challenges attributable to its inherent instability and susceptibility to *** its elevated calcium sulfate content,ABR exhibits considerable promise for industrial *** study delved into the feasibility of utilizing ABR as a source of sulfates for producing super sulfated cement(SSC),offering an innovative binder for cemented paste backfill(CPB).Thermal treatment at varying temperatures of 150,350,600,and 800℃ was employed to modify ABR’s *** investigation encompassed the examination of phase transformations and alterations in the chemical composition of As within ***,the hydration characteristics of SSC utilizing ABR,with or without thermal treatment,were studied,encompassing reaction kinetics,setting time,strength development,and *** findings revealed that thermal treatment changed the calcium sulfate structure in ABR,consequently impacting the resultant sample ***,calcination at 600℃ demonstrated optimal modification effects on both early and long-term strength *** enhanced performance can be attributed to the augmented formation of reaction products and a densified ***,the thermal treatment elicited modifications in the chemical As fractions within ABR,with limited impact on the As immobilization capacity of the prepared binders.

关键词： cemented paste backfill bio-leaching residue arsenic immobilization binder hydration calcination

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Automatic modeling algorithm of stochastic error for inertial sensors

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Control Theory and Technology 2024年第1期22卷 81-91页

作者： Luodi zhao Long zhao School of Automation Science and Electrical Engineering Beihang UniversityBeijing100191China Digital Navigation Center Beihang UniversityBeijing100191China Science and Technology on Aircraft Control Laboratory Beihang UniversityBeijing100191China

This paper proposes an automatic algorithm to determine the properties of stochastic processes and their parameters for inertial error. The proposed approach is based on a recently developed method called the generalized method of wavelet moments (GMWM), whose estimator was proven to be consistent and asymptotically normally distributed. This algorithm is suitable mainly (but not only) for the combination of several stochastic processes, where the model identification and parameter estimation are quite difficult for the traditional methods, such as the Allan variance and the power spectral density analysis. This algorithm further explores the complete stochastic error models and the candidate model ranking criterion to realize automatic model identification and determination. The best model is selected by making the trade-off between the model accuracy and the model complexity. The validation of this approach is verified by practical examples of model selection for MEMS-IMUs (micro-electro-mechanical system inertial measurement units) in varying dynamic conditions.

关键词： GMWM Stochastic process Inertial sensor Sensor calibration Error model Allan variance

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Meeting the challenges of invasive alien species

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National Science Review 2024年第3期11卷 296-298页

作者： Weijie zhao NSR news

The Xinjiang Uygur Autonomous Region of China is famous for its tomato production. Abundant sunshine, large day–night temperature difference and the abundant irrigation by snowmelt from the Tianshan Mountains produce...

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Ling-Ling Chen: RNA has its own features;don't study it as a protein

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National Science Review 2024年第2期11卷 301-305页

作者： Weijie zhao NSR

Non-coding RNA(ncRNA) has been a very active research area over the past 30 years. From small nc RNA – the discovery of RNA interference won the lead researchers the Nobel Prize, to long ncRNA(lncRNA), which has attracted much attention in recent years, various nc RNAs participate in all kinds of biological processes and show a variety of biomedical application prospects.

关键词： RNA

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Giant Flexoelectric Effect in Snapping Surfaces Enhanced by Graded Stiffness

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Acta Mechanica Solida Sinica 2024年第4期37卷 528-540页

作者： Chuo zhao Department of Aeronautics Imperial College LondonSouth Kensington CampusLondonSW72AZUK

Flexoelectricity is present in nonuniformly deformed dielectric materials and has size-dependent properties, making it useful for microelectromechanical systems. Flexoelectricity is small compared to piezoelectricity;therefore, producing a large-scale flexoelectric effect is of great interest. In this paper, we explore a way to enhance the flexoelectric effect by utilizing the snap-through instability and a stiffness gradient present along the length of a curved dielectric plate. To analyze the effect of stiffness profiles on the plate, we employ numerical parameter continuation. Our analysis reveals a nonlinear relationship between the effective electromechanical coupling coefficient and the gradient of Young’s modulus. Moreover, we demonstrate that the quadratic profile is more advantageous than the linear profile. For a dielectric plate with a quadratic profile and a modulus gradient of − 0.9, the effective coefficient can reach as high as 15.74 pC/N, which is over three times the conventional coupling coefficient of piezoelectric material. This paper contributes to our understanding of the amplification of flexoelectric effects by harnessing snapping surfaces and stiffness gradient design.

关键词： Functionally graded material Flexoelectricity Numerical continuation Snap-through instability

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面向阵列级同时同频收发的宽带自干扰空域抑制

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电子与信息学报 2024年第5期46卷 1951-1957页

作者：林朗赵宏志邵士海唐友喜电子科技大学通信抗干扰全国重点实验室成都611731 电磁空间认知与智能控制技术实验室北京100089

具备阵列级同时同频收发能力的多功能一体化平台,面临发射阵列对接收阵列的强自干扰问题。针对这一问题,该文设计了一种宽带自干扰空域抑制方法,构造了限制主波束增益损失条件下,最小化残余自干扰和噪声功率的非凸优化问题,提出了交替... 详细信息

具备阵列级同时同频收发能力的多功能一体化平台,面临发射阵列对接收阵列的强自干扰问题。针对这一问题,该文设计了一种宽带自干扰空域抑制方法,构造了限制主波束增益损失条件下,最小化残余自干扰和噪声功率的非凸优化问题,提出了交替迭代的优化算法联合求解发射和接收数字波束成形系数,从理论上分析了所提算法的自干扰抑制极限性能,并给出了所提算法的计算复杂度。分析和仿真结果表明,给定2.4 GHz工作频率,100 MHz带宽,主波束增益损失限制在3 dB时,收发各30阵元的相控阵列宽带自干扰空域抑制能力,即有效各向同性隔离度达到168 dB,距离自干扰抑制性能极限差距7 dB。

关键词：同时同频收发数字波束成形空域自干扰抑制

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One dimensional CeO_(2) nanorods/poly(ethylene oxide) solid composite electrolyte for all-solid-state lithium-ion batteries

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Journal of Rare Earths 2024年第3期42卷 570-577,I0005页

作者： Yudi Guo Erqing zhao Xiaofang zhao Shuailei Liu School of Chemistry and Materials Engineering Xinxiang UniversityXinxiang453003China School of Chemistry and Chemical Engineering Henan Institute of Science and TechnologyXinxiang453003China

The research of poly(ethylene oxide)(PEO)-based solid composite electrolyte with high ionic conductivity and excellent interfacial stability is the key to the development of all-solid-state lithium-ion batteries(ASSLIBs). Herein, uniform nanorod structured CeO_(2) fillers were controllably synthesized by electrospinning, which were subsequently filled into PEO polymer to prepare CeO_(2)/PEO solid composite electrolyte. The addition of CeO_(2) nanorods can reduce both the glass transition temperature and the melting point of PEO polymer, and also interact with PEO and lithium bis(trifluoromethanesulphonyl)imide(LITFSI) by Lewis acid—base reaction. Therefore, the solid composite electrolyte exhibits a high ionic conductivity of 4.52 × 10^(-4)S/cm, a wide electrochemical stability window of about 4.8 V, and a good interfacial stability with Li at 55℃. Moreover, the LiFePO_4/Li ASSLIB divulges the discharging specific capacity of 165, 162, 156 and 146 mA,h/g at 0.2, 0.5, 1 and 2 C, respectively, and achieves the capacity retention of 90.3% after 150 cycles at 0.5 C. Consequently, one dimensional CeO_(2) nanorods can be considered as an alternative filler for polymeric solid electrolyte.

关键词： Poly(ethylene oxide) Solid composite electrolyte CeO_(2)nanorods Ionic conductivity Rare earths

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The trajectory of rice from wild to domesticated:A new chapter in the study of origins of agriculture

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Science China Earth Sciences 2024年第9期67卷 3011-3012页

作者： Zhijun zhao Institute of Cultural Heritage Shandong UniversityQingdao 266237China

Rice(Oryza sativa)is one of the most important food crops in the world today,feeding more than one-third of the global *** domestication of rice has always been an international research topic(Gross and zhao,2014).When,where,and how rice was cultivated and eventually domesticated has been a hot issue in many research fields such as archaeology,biology,and agronomy(Fuller et al.,2009).

关键词： sativa Oryza crops

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