High level expression of Saccharomyces cerevisiae chitinase(ScCTS1)in Pichia pastoris for degrading chitin

作     者：Zhao Youxi Jiang Huihui Rao Zhiming Ji Yizhi Cheng Yanling Ma Yanhe 

作者机构：Key Laboratory of Industrial BiotechnologyMinistry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Beijing Key Laboratory of Biomass Waste Resource UtilizationBiochemical Engineering CollegeBeijing Union UniversityBeijing 100023China State Key Laboratory of Microbial ResourcesInstitute of MicrobiologyChinese Academy of SciencesBeijing 100101China 

出 版 物：《International Journal of Agricultural and Biological Engineering》 (国际农业与生物工程学报（英文）)

年 卷 期：2015年第8卷第5期

页      面：142-150页

核心收录：

学科分类：0710[理学-生物学] 0810[工学-信息与通信工程] 1002[医学-临床医学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 10[医学] 

基　　金：This work was supported by the General Project of Beijing Municipal Education Commission(No.SQKM201311417003) Beijing Excellent Talents Cultivation Project(No.2012D005022000007) Ministry of Science and Technology“863 Plan”Project(No.2015AA020202) 

主　　题：chitinases heterologous expression Saccharomyces cerevisiae pichia pastoris biotechnology 

摘      要：Chitin is the second most abundant renewable biopolymer in the *** play important roles in the degradation of *** are produced by different organisms for different purposes,which are widely expressed in the three domains of life,ranging from archaea,bacteria,to fungi,yeasts,plants,insects,and even *** there are few reports about Saccharomyces cerevisiae chitinase(ScCTS1).The aim of this study was to realize the high level expression of *** ScCTS1 was cloned into the expression vector *** recombinant plasmid was linearized and transformed into competent Pichia pastoris *** screening by G418 plate,the fermentation conditions were ***,under the optimal fermentation conditions,ScCTS1 enzymatic activity reached up to 94.6 U/*** paper presents the first report on the heterologous expression of a full-length ScCTS1 with considerably high *** work will not only make a great stride towards its potential applications in biotechnology,but also facilitate elucidating the precise mechanism of yeast cell division.