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Gene Expression of Ornithine Decarboxylase in Lung Cancers and Its Clinical Significance

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 639-645页

作者： Hui TIAN~1* Qing HUANG~1 Lm LI~1 Xian-Xi LIU~2 yan zhang~2 1 Department of Thoracic Surgery,Shandong University Qi Lu Hospital,Jinan 250012,China 2 Department of Medicine,Medical Molecular Biology Experimental Center,Shandong University,Jinan 250012,China Department of Thoracic Surgery Shandong University Qi Lu HospitalJinan 250012 China Department of Medicine Medical Molecular Biology Experimental Center Shandong UniversityJinan 250012 China

Lung cancer is one of the most lethal cancers in China because of its high incidence and *** decarboxylase （ODC）,an important enzyme in polyamine biosynthesis,is increased incancer *** chemotherapeutic agents aimed at reducing ODC expression show inhibitory effects oncancer cell growth,so ODC can be useful in gene diagnosis and gene therapy of *** this study,weexamined the effect of antisense ODC on lung cancer cells.A-549 cells were infected with rAd-ODC/Ex3as,a recombinant adenovirus containing the cytomegalovirus promoter,green fluorescent protein gene and 120bp antisense *** cell cycle was evaluated by flow cytometry.A nude mouse xenograft model wasused in the tumorigenicity *** transcription-polymerase chain reaction,Western blot and immuno-histochernistry were used to study the expressions of ODC on lung *** was found that the growth ofcells infected with rAd-ODC/Ex3as was substantially inhibited and cells were arrested at *** with rAd-ODC/Ex3as can suppress tumor formation in a nude mouse xenograft *** of ODC mRNA and ODC protein levels in lung cancer tissues was significantly higher than thatin normal tissues （Pyan important role in lung cancer and the overexpression of ODC might be related to the occurrence anddevelopment of lung cancer.

关键词： polyamine biosynthesis lung neoplasm ornithine decarboxylase A-549 cell line

Effects of scandium addition on microstructure and mechanical properties of ZK60 alloy

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Progress in Natural Science:Materials International 2011年第1期21卷 59-65页

作者： Fu-sheng PAN~(1,2),Ting-ting LIU~(1,2),Xi-yan zhang~(1,2),Ai-tao TANG~(1,2),Wei-qing WANG~(1,2) 1.National Engineering Research Center for Magnesium Alloys,Chongqing University,Chongqing 400045,China 2.College of Materials Science and Engineering,Chongqing University,Chongqing 400045,China National Engineering Research Center for Magnesium Alloys Chongqing University Chongqing 400045 China College of Materials Science and Engineering Chongqing University Chongqing 400045 China

The effects of Sc addition on the microstructure and mechanical properties of the ZK60 wrought magnesium alloy were investigated by using optical microscope,scanning electron microscopy,X-ray diffraction and tensile *** experimental results show that a minor Sc addition to ZK60 alloy has an obvious effect on the refinement of the microstructure of the ZK60 alloy. During hot extrusion,incomplete dynamic recrystallization occurs in all the alloys,and the recrystallized grains become much finer with increasing Sc *** tensile strengths of ZK60 evidently increase with the addition of Sc,but the elongations decrease. The ZK60 alloy with 0.6%(mass fraction) Sc addition is found to have the tensile strength of about 350 MPa and the yield strength of about 280 *** T6 heat treatment,the tensile strength of the alloy containing 0.6%Sc remains almost ***, the yield strength of the alloy increases up to 352 MPa,and the ratio of the tensile strength to the yield strength is close to 1.

关键词： wrought magnesium alloy scandium microstructure mechanical properties

Antitumor Effect of Antisense Ornithine Decarboxylase Adenovirus on Human Lung Cancer Cells

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Acta Biochimica et Biophysica Sinica 2006年第6期38卷 410-416页

作者： Hui TIAN~(1*),Lin LI~1,Xian-Xi LIU~2,and yan zhang~2 ~1 Department of Thoracic Surgery,Shandong University Qi Lu Hospital,Jinan 250012,China ~2 Experimental Center of Medical Molecular Biology,School of Medicine,Shandong University,Jinan 250012,China Department of Thoracic Surgery Shandong University Qi Lu HospitalJinan 250012 China Experimental Center of Medical Molecular Biology School of Medicine Shandong UniversityJinan 250012 China

Ornithine decarboxylase (ODC),the first enzyme of polyamine biosynthesis,was found toincrease in cancer cells,especially lung cancer *** chemotherapeutic agents aimed at decreasingODC gene expression showed inhibitory effects on cancer cells,ha this study,we examined the effects ofadenoviral transduced antisense ODC on lung cancer *** adenovirus carrying antisense ODC(rAd-ODC/Ex3as) was used to infect lung cancer cell line *** 3-(4,5-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was used to analyze the effect on cell *** of ODC and concentrationof polyamines in cells were determined by Western blot analysis and high performance liquid *** deoxynucleotidyl transferase-mediated biotin-dUTP nick-end labeling was used to analyze *** expression of ODC in A-549 cells was reduced to 54%,and that of three polyamines wasalso decreased through the rAd-ODC/Ex3as ***,cell growth was substantially inhibitedand terminal deoxynucleotidyl transferase-mediated biotin-dUTP nick-end labeling showed that rAd-ODC/Ex3ascould lead to cell apoptosis,with apoptosis index of 46%.This study suggests that rAd-ODC/Ex3as has anantitumor effect on the human lung cancer cells.

关键词： polyamine putrescine biosynthesis antisense technology gene therapy A-549 lung cancer cell line

Experimental research on TECA-I bioartificial liver support system to treat canines with acute liver failure

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World Journal of Gastroenterology 2001年第5期7卷 706-709页

作者： Xiao-Ping Chen~2 Yi-Long Xue~1 Xin-Jian Li~1 Zuo-Yun zhang~1 yan-Ling Li~1 Zhi-Qiang Huang~2 1 Institute of Basic Medical Sciences,2 Department of Hepatobiliary Surgery,PLA General Hospital,Beijing 100853,China Institute of Basic Medical Sciences PLA General Hospital Beijing 100853 China.

AIM: To evaluate the efficacy and safety of the TECA-I bioartificial liver support system (BALSS) in treating canines with acute liver failure (ALF). METHODS: Ten canines with ALF induced by 80% liver resection received BALSS treatment (BALSS group). Blood was perfused through a hollow fiber tube containing 1X10(10) porcine *** canines with ALF were treated with BALSS without porcine hepatocytes (control group), and five canines with ALF received drug treatment (drug group). Each treatment lasted 6 hours. RESULTS: BALSS treatment yielded beneficial effects for partial liver resection induced ALF canines with survival and decreased plasma ammonia, ALT, AST and BIL. There was an obvious decrease in PT level and increase in PA level, and there were no changes in the count of lymphocytes, immunoglobulins (IgA, IgG and IgM) and complement (C3 and C4) levels after BALSS treatment. In contrast, for the canines with ALF in non-hepatocyte BALSS group (control group) and drug group, there were no significant changes in ammonia, ALT, AST, BIL, PT and PA levels. ALF canines in BALSS group, control group and drug group lived respectively an average time of 108.0h +/- 12.0h, 24.0h +/- 6.0h and 20.4h +/- 6.4h,and three canines with ALF survived in BALSS group. CONCLUSION: TECA-I BALSS is efficacious and safe for ALF canines induced by partial liver resection.

关键词： Liver, Artificial Animals Blood Coagulation Dogs Hepatectomy Hepatocytes Kidney Liver Liver Failure, Acute Lung Myocardium Swine

Liver metastasis models of human colorectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristics

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World Journal of Gastroenterology 1998年第5期4卷 46-48页

作者： LIU Qiu Zhen 1, TUO Chao Wei 1, WANG Bin 1, WU Bing Quan 2 and zhang yan Hua 1

AIM To establish a liver metastasis model of human colorectal carcinoma in nude *** Orthotopic transplantation of histologically intact colorectal tissues from patients into colorectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and *** Liver metastasis models of human colon carcinoma (HCA-HMN-1) and human rectal carcinoma (HRA-HMN-2) were established after screening from 34 colorectal carcinomas. They had been passaged in vivo for 18 and 21 generations respectively. There were lymphatic, hemotogenous and implanting metastasesis. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal *** The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colorectal *** models of nude mice that fresh human colorectal carcinoma tissue or cells were successfully transplanted subcuteneously have been reported at home and abroad[1,2]. But until now there has been no report on a liver metastasis model of human colorectal carcinoma established by orthotopic transplantation in nude mice in China. Based on our previous models of human liver and pancreas carcinoma by orthotopic transplantation[3,4], we established liver metastasis models of colon and rectum carcinoma with a spontaneous metastasis rate of 100%.

关键词： colorectal neoplasms liver neoplasms/secondary neoplasms transplatation neoplasms metastasis disease models, animal

Immunization with autologous T cells enhances in vivo anti-tumor immune responses accompanied by up-regulation of GADD45β

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Cell Research 2006年第8期16卷 702-712页

作者： Li Wang~(1,2,*) Fang Du~(1,2,*) Qi Cao~(1,2) Huiming Sheng~(1,2) Baihua Shen~(1,2) yan zhang~(1,2) Yingna Diao~(1,2) Jingwu zhang~(1,2) Ningli Li~(1,2) ~1Shanghai diao Tong UniversiO,School of Medicine,280 Chongqing Road,Shanghai 200025,China ~2Shanghai Institute of Immunology,280 Chongqing Road,Shanghai 200025,China Shanghai Jiao Tong University School of Medicine Shanghai China Shanghai Institute of Immunology Shanghai China

Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreac-tire T cells,which are involved in autoimmune ***,it is unknown whether attenuated activated healthyautologous T-cell immunization could increase anti-tumor immune *** this end,C57B1/6 mice were immunizedwith attenuated activated autologous T *** splenocytes from immunized mice showed a higher proliferative abil-ity than that from naive *** special phenotype analysis showed that there were more CDS+T cells and CD62L+T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro(P1).Theseresults demonstrated that this immunization may activate T cells in ***,the splenocytes from immunizedmice revealed resistance to activation-induced cell death(AICD)in *** further study the relative genes that areresponsible for the higher proliferation and resistance to AICD,the expression of Fas/Fas ligand(FasL)and GADD45βwas measured by real-time *** results indicated that GADD45β transcription was higher in the splenocytes fromimmunized mice than that in the naive *** addition,the Fas expression showed a parallel higher,but FasL did notchange *** investigate the biologic functions induced by immunization in vivo,a tumor model was establishedby EL-4 tumor cell inoculation in C57/B1 *** receiving autologous T-cell immunization had significantly inhibitedtumor growth in vivo(P1).This study implicated that immunization with attenuated activated autologous T cellsenhances anti-tumor immune responses that participate in tumor growth inhibition.

关键词： anti-tumor immunity immunization autologous T cells GADD45β AICD

Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery

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Cell Research 2006年第6期16卷 599-608页

作者： Xin Yu~1 yan Hui Peng~1 Min Hua zhang~1 yan Jun Shao~(1,2) Wei Ai Su~1 zhang Cheng Tang~1 Institute of Plant Physiology and Ecology,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences 300 Fenglin Road,Shanghai,200032,China ~2Life Science College,Hebei Agriculture University,38 Ling-Yu-Si street,Baoding,Hebei,071001,China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Life Science College Hebei Agriculture University Baoding China

A symptom of chilling injury is development of water deficit in shoots,resulting from an imbalance of water transportand *** this work,two rice varieties(Oryza sativa *** and Somewake)seedlings were chilledat 7℃,followed by recovery at 28℃.Based on the growth phenotype and electrolyte leakage tests,Somewake wasshown to be a chilling-tolerant variety,and Wasetoitsu a chilling-sensitive *** chilling stress reduced markedly therelative water content(RWC)of leaves,accumulative transpiration and osmotic root hydraulic conductivity(Lp)in *** when returned to 28℃,the water relation balance of Somewake recovered *** mRNA expressionprofile of all the 11 plasma membrane intrinsic proteins(PIPs),a subgroup of aquaporins,was subsequently determinedby real-time reverse transcription(RT)-PCR with TaqMan-minor grove binder(MGB)probes derived from rice ***-ponbare during chilling treatment and *** of the PIP genes was down-regulated at the low temperature,andrecovered at the warm *** relative expression of some PIPs in both Somewake and Wasetoitsu decreasedin parallel during the *** during the recovery,the relative expression ofOsPIPl;1,OsPIP2,1,OsPIP2;7in shoots and OsPIP1;1,OsPIP2;1 in roots were significantly higher in Somewake than *** supports therole of PIPs in re-establishing water balance after chilling *** discuss the diversified roles played by membersof the aquaporin PIP subfamily in plant chilling tolerance depending on aquaporin isoforms,plant tissue and the stageof chilling duration.

关键词： aquaporin chilling gene expression plasma membrane intrinsic protein rice

Curve fitting and optimal interpolation on CNC machines based on quadratic B-splines

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Science China(Information Sciences) 2011年第7期54卷 1407-1418页

作者： zhang Mei 1,yan Wei 2,YUAN ChunMing 1,WANG DingKang 1 & GAO XiaoShan 1 1 Key Laboratory of Mathematics Mechanization,Academy of Mathematics and Systems Science,Chinese Academy of Sciences,Beijing 100190,China 2 Research Institute of Petroleum Exploration and Development,Beijing 100083,China Key Laboratory of Mathematics Mechanization Academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing China Research Institute of Petroleum Exploration and Development Beijing China

In this paper,curve fitting of 3-D points generated by G01 codes and interpolation based on quadratic B-splines are *** points of G01 codes are selected using an adaptive ***,quadratic B-splines are obtained as the fitting curve by interpolating these feature *** required in implementing the velocity planning algorithm mentioned by Timer et *** very complicated because of the appearance of high-order *** improved time-optimal method for the quadratic B-spline curves is presented to circumvent this *** algorithms are verified with simulations as well as on real CNC machines.

关键词： quadratic B-spline G01 codes feature point velocity planning interpolation

Development of solar-blind AlGaN 128x128 Ultraviolet Focal Plane Arrays

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Science China(Technological Sciences) 2008年第6期51卷 820-826页

作者： YUAN YongGang1, zhang yan1, CHU KaiHui1, LI Xiangyang1, ZHAO DeGang2 & yanG Hui2 1 State Key Laboratories of Transducer Technology, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China 2 Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China State Key Laboratories of Transducer Technology Shanghai Institute of Technical Physics Chinese Academy of Sciences Shanghai China Institute of Semiconductors Chinese Academy of Sciences Beijing China

This paper reports the development of solar-blind aluminum gallium nitride (AlGaN) 128×128 UV Focal Plane Arrays (FPAs). The back-illuminated hybrid FPA archi- tecture consists of an 128x128 back-illuminated AlGaN PIN detector array that is bump-mounted to a matching 128x128 silicon CMOS readout integrated circuit (ROIC) chip. The 128×128 p-i-n photodiode arrays with cuton and cutoff wave-lengths of 233 and 258 nm, with a sharp reduction in response to UVB (280―320 nm) light. Several examples of solar-blind images are provided. This solar-blind band FPA has much better application prospect.

关键词： AlGaN photodiode ultraviolet FPA solar-blind

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