Immunization with autologous T cells enhances in vivo anti-tumor immune responses accompanied by up-regulation of GADD45β

作     者：Li Wang~(1,2,*) Fang Du~(1,2,*) Qi Cao~(1,2) Huiming Sheng~(1,2) Baihua Shen~(1,2) Yan Zhang~(1,2) Yingna Diao~(1,2) Jingwu Zhang~(1,2) Ningli Li~(1,2) ~1Shanghai diao Tong UniversiO,School of Medicine,280 Chongqing Road,Shanghai 200025,China ~2Shanghai Institute of Immunology,280 Chongqing Road,Shanghai 200025,China 

作者机构：Shanghai Jiao Tong University School of Medicine Shanghai China Shanghai Institute of Immunology Shanghai China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2006年第16卷第8期

页      面：702-712页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

主　　题：anti-tumor immunity immunization autologous T cells GADD45β AICD 

摘      要：Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreac-tire T cells,which are involved in autoimmune ***,it is unknown whether attenuated activated healthyautologous T-cell immunization could increase anti-tumor immune *** this end,C57B1/6 mice were immunizedwith attenuated activated autologous T *** splenocytes from immunized mice showed a higher proliferative abil-ity than that from naive *** special phenotype analysis showed that there were more CDS+T cells and CD62L+T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro(P0.01).Theseresults demonstrated that this immunization may activate T cells in ***,the splenocytes from immunizedmice revealed resistance to activation-induced cell death(AICD)in *** further study the relative genes that areresponsible for the higher proliferation and resistance to AICD,the expression of Fas/Fas ligand(FasL)and GADD45βwas measured by real-time *** results indicated that GADD45β transcription was higher in the splenocytes fromimmunized mice than that in the naive *** addition,the Fas expression showed a parallel higher,but FasL did notchange *** investigate the biologic functions induced by immunization in vivo,a tumor model was establishedby EL-4 tumor cell inoculation in C57/B1 *** receiving autologous T-cell immunization had significantly inhibitedtumor growth in vivo(P0.01).This study implicated that immunization with attenuated activated autologous T cellsenhances anti-tumor immune responses that participate in tumor growth inhibition.