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Wnt3a signaling promotes proliferation,myogenic differentiation,and migration of rat bone marrow mesenchymal stem cells

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Acta Pharmacologica Sinica 2007年第11期28卷 1761-1774页

作者： Yan-chang SHANG~(2.3),Shu-hui WANG~4,Fu XIONG~5,Cui-ping ZHAO~2.Fu-ning PENG~2.Shan-wei FENG~2.Mei-shan LI~2.YongLI~5,Cheng zhang~(2.5,6)~2.department of Neurology First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,{2.,~3 department of Geriatric NeurologyChinese People's Liberation Army General Hospital,Beijing 100853,{2. ~4 department of Neurology,Beijing Friendship Hospital,CapitalMedical University,Beijing 100050,{2.,~5 Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University Guangzhou510080.{2. ment of Neurology First Affiliated Hospital Sun Yat-sen University Guangzhou 510080China. zhangch6@***.

Aim:To investigate the effects of the wingless-related MMTV integration site 3A(Wnt3a) signaling on the proliferation,migration,and the myogenic and adipogenicdifferentiation of rat bone marrow mesenchymal stem cells (rMSC).Methods:Primary MSC were isolated and cultured from Sprague-Dawley rats and character-ized by flow *** L cells were transfected with Wnt3a cDNA,andconditioned media containing active Wnt3a proteins were *** prolif-eration was evaluated by cell count and 5-bromodeoxyuridine incorporation *** migration of rMSC was performed by using a transwell migration and woundhealing *** myogenic and adipogenic differentiation in rMSC were exam-ined by light microscopy,immunofluorescence,and RT-PCR at different timepoints after myogenic or adipogenic ***:Wnt3a signaling in-duced β-catenin nuclear translocation and activated the Wnt pathway in *** the presence of Wnt3a,rMSC proliferated more rapidly than the control cells,keeping their differentiation ***,Wnt3a signaling induced 2.62.and 3.76% of rMSC-expressed desmin and myosin heavy chain after being cul-tured in myogenic *** myogenic differentiation genes,including Pax7,MyoD,Myf5,Myf4,and myogenin,were activated after Wnt3a *** theother hand,Wnt3a inhibited the adipogenic differentiation in rMSC through thedownregulated expression of CCAAT/enhancer-binding protein alpha (C/EBPalpha)and peroxisome proliferator-activated receptor gamma (PPARgamma).Furthermore,Wnt3a promoted the migration capacity of ***:The results indi-cate that Wnt3a signaling can induce myogenic differentiation in ***3asignaling is also involved in the regulation of the proliferation and migration *** results could provide a rational foundation for cell-based tissuerepair in humans.

关键词： Wingless-related MMTV integration site (Wnt) mesenchymal stem cells myogenic differentiation adipogenic differentiation proliferation migration

An efficient and safe strategy for germ cell-specific automatic excision of foreign DNA in F1 hybrid transgenic silkworms

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Insect Science 2024年第1期31卷 28-46页

作者： Dingpei Long Rongpeng Liu Yang Huang Anyao Fu Yuli zhang Zhanzhang Hao Qiang Li Hanfu Xu Zhonghuai Xiang Aichun Zhao State Key Laboratory of Resource Insects Institute of Sericulture and Systems BiologySouthwest UniversityChongqingChina department of Biology Georgia State UniversityAtlantaGeorgiaUSA Guangxi Institute of Sericulture Science NanningGuangxiChina department of mathematics and Statistics Georgia State UniversityAtlantaGeorgiaUSA

The safety of transgenic technology is a major obstacle in the popularization and use of transgenic silkworms and their *** sericulture,only the first filial generation(F1)hybrid eggs produced by cross-breeding Japanese and Chinese original strains are usually used for the large-scale breeding of silkworms,but this may result in uncontrolled transgene dispersal during the popularization and application of the F1 hybrid transgenic *** address this issue,we developed a safe and efficient strategy using the GAL4/Upstream activating sequence(UAS)system,the FLP/flippase recognition target(FRT)system,and the gonad-specific expression gene promoters(RSHP1p and Nanosp)for the germ cell-specific automatic excision of foreign DNA in the F1 hybrid transgenic *** established 2.types of activator strains,R1p::GAL4-Gr and Nsp::GAL4-Gr,containing the testis-specific GAL4 gene expression cassettes driven by RSHP1p or Nanosp,respectively,and 1 type of effector strain,UAS::FLP-Rg,containing the UAS-linked FLP gene expression *** FLP recombinase-mediated sperm-specific complete excision of FRT-flanked target DNA in the F1 double-transgenic silkworms resulting from the hybridization of R1p::GAL4-Gr and UAS::FLP-Rg was 100%,whereas the complete excision efficiency resulting from the hybridization of Nsp::GAL4-Gr and UAS::FLP-Rg ranged from 13.73%to 80.3%.Additionally,we identified a gene,sw11114,that is expressed in both testis and ovary of Bombyx mori,and can be used to establish novel gonad-specific expression systems in transgenic *** strategy has the potential to fundamentally solve the safety issue in the production of F1 transgenic silkworm eggs and provides an important reference for the safety of transgenic technology in other insect species.

关键词： Bombyx mori FLP/FRT system GAL4/UAS system germ cell-specific excision safety of transgenic technology transgenic silkworm

MFG-E8 Alleviates Cognitive Impairments Induced by Chronic Cerebral Hypoperfusion by Phagocytosing Myelin Debris and Promoting Remyelination

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Neuroscience Bulletin 2024年第4期40卷 483-499页

作者： Xiaohong Dong Zhi zhang Xin Shu Zi Zhuang Pinyi Liu Renyuan Liu Shengnan Xia Xinyu Bao Yun Xu Yan Chen department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing210008China department of Neurology Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing210008China department of Neurology Drum Tower Hospital of Nanjing Medical UniversityNanjing210008China Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjing210008China Jiangsu Provincial Key Discipline of Neurology Nanjing210008China

Chronic cerebral hypoperfusion is one of the pathophysiological mechanisms contributing to cognitive decline by causing white matter *** phagocytosing myelin debris in a timely manner can promote remyelination and contribute to the repair of white ***,milk fat globule-epidermal growth factor-factor 8(MFG-E8),a microglial phagocytosis-related protein,has not been well studied in hypoperfusion-related cognitive *** found that the expression of MFG-E8 was significantly decreased in the brain of mice after bilateral carotid artery stenosis(BCAS).MFG-E8 knockout mice demonstrated more severe BCAS-induced cognitive impairments in the behavioral *** addition,we discovered that the deletion of MFG-E8 aggravated white matter damage and the destruction of myelin microstructure through fluorescent staining and electron ***,MFG-E8 overexpression by AAV improved white matter injury and increased the number of mature oligodendrocytes after ***,in vitro and in vivo experiments showed that MFG-E8 could enhance the phagocytic function of microglia via theαVβ3/αVβ5/Rac1 pathway and IGF-1 production to promote the differentiation of oligodendrocyte progenitor cells into mature ***,we found that MFG-E8 was mainly derived from astrocytes,not *** findings suggest that MFG-E8 is a potential therapeutic target for cognitive impairments following cerebral hypoperfusion.

关键词： White matter injury Cognitive dysfunction MFG-E8 Remyelination Microglial phagocytosis

Cross metathesis of butene-2.and ethene to propene overMo/MCM-2.-Al_2._3 catalysts with different Al_2._3 contents

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Journal of Natural Gas Chemistry 2010年第5期19卷 482-486页

作者： Shenglin Liu Xiujie Li Wenjie Xin Sujuan Xie Peng Zeng lixin zhang Longya Xu Dalian Institute of Chemical Physics The Chinese Academy of Sciences Dalian 116023 Liaoning China HQCEC （Liaoning） CO. Fushun 113008 Liaoning China Liaoning Petrochemical Technology College Jinzhou 121001 Liaoning China

A series of 3.0Mo/MCM-2.-Al2.3 catalysts with γ-Al2.3 contents in the range of 0-100 wt% were prepared and applied in the metathesis reaction of ethene and butene-2. Addition of γ-Al2.3did not affect the structure of MCM-2. zeolite as evidenced by XRD and N2.adsorption measurements. It was deduced from TPR experiments that γ-Al2.3 phase favored the formation of polymolybdate or multilayered Mo oxide, while more Al2.MoO4）3 species were generated over MCM-2. zeolites. Alumina content in the support was directly related to the metathesis activity of ethene and butene-2.to propene. Mo species with higher valence （Mo6＋or Mo5＋） contributed more to the excellent performance of catalyst than metallic Mo. The best catalyst activity and stability was obtained over 3.0Mo/（MCM-2.-30%Al2.3） under the reaction condition of 1.0 MPa and 12.℃ using N2.as the pretreatment gas.

关键词： butene-2 ethene propene metathesis Mo MCM-2.-Al2.3

MRI Findings and Diagnostic Significance of Soft Tissue Schwannomas in the Extremities

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Journal of Clinical and Nursing Research 2024年第8期8卷 31-36页

作者： Guoping zhang Xiaoli Chen Center of Diagnostic Medical Imaging Shaanxi Provincial People’s HospitalXi’an 710068Shaanxi ProvinceChina department of Neurology Shaanxi Provincial People’s HospitalXi’an 710068Shaanxi ProvinceChina

Objective:To analyze the MRI features and rare manifestations of schwannomas of the extremities to improve ***:Retrospective analysis of 2. cases of schwannomas in the extremities confirmed by surgical pathology,summarizing its signs,signal characteristics and MRI ***:Among the 2. cases,2. were single lesions,and 3 were multiple lesions,resulting in a total of 2. *** distribution of these lesions included 7 in the thigh,9 in the calves,4 in the armpits,3 in the hips,and 4 each in the upper arms and ***,there was one lesion located in the *** T1-weighted imaging(T1WI),the lesions showed iso-intensity or *** T2.weighted imaging(T2.I),they appeared slightly hyperintense or *** lesions exhibited multiple small vesicle-like higher signals,cystic changes,and fiber spacing on ***,one lesion showed a liquid-liquid ***“target sign”was observed in 18 lesions(64.3%)and“neuropathic signs”were present in six lesions(2..4%).Finally,it was noted that on MRI enhancement,the lesions demonstrated uniform or uneven *** lesions were uniformly or unevenly enhanced on MRI ***:The MRI findings of schwannoma in the extremities exhibit specific *** cystic changes on T2.I also display distinct *** of rare MRI findings,such as cystic changes,liquid-liquid plane and septum,should be emphasized to enhance the accuracy of pre-diagnosing schwannomas.

关键词： Schwannomas Extremities Magnetic resonance imaging

Exploration of transferable deep learning-aided radio frequency fingerprint identification systems

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Security and Safety 2024年第1期3卷 7-20页

作者： Guanxiong Shen Junqing zhang department of Electrical Engineering and Electronics University of LiverpoolLiverpool L693GJUK

Radio frequency fingerprint identification(RFFI)shows great potential as a means for authenticating wireless *** RFFI can be addressed as a classification problem,deep learning techniques are widely utilized in modern RFFI systems for their outstanding *** is suitable for securing the legacy existing Internet of Things(IoT)networks since it does not require any modifications to the existing end-node hardware and communication ***,most deep learning-based RFFI systems require the collection of a great number of labelled signals for training,which is time-consuming and not ideal,especially for the Io T end nodes that are already deployed and configured with long transmission ***,the long time required to train a neural network from scratch also limits rapid deployment on legacy Io T *** address the above issues,two transferable RFFI protocols are proposed in this paper leveraging the concept of transfer *** specifically,they rely on fine-tuning and distance metric learning,respectively,and only require only a small amount of signals from the legacy IoT *** the dataset used for transfer is small,we propose to apply augmentation in the transfer process to generate more training signals to improve performance.A Lo Ra-RFFI testbed consisting of 40 commercial-off-the-shelf(COTS)Lo Ra IoT devices and a software-defined radio(SDR)receiver is built to experimentally evaluate the proposed *** experimental results demonstrate that both the fine-tuning and distance metric learning-based RFFI approaches can be rapidly transferred to another Io T network with less than ten signals from each Lo Ra *** classification accuracy is over 90%,and the augmentation technique can improve the accuracy by up to 2.%.

关键词： Device authentication internet of things LoRa radio frequency ngerprint identi cation deep learning wireless security

Activation of LONP1 by 84-B10 alleviates aristolochic acid nephropathy via re-establishing mitochondrial and peroxisomal homeostasis

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Chinese Journal of Natural Medicines 2024年第9期22卷 808-821页

作者： XU Xinyue ZHU Wenping MIAO Mengqiu BAI Mi FAN Jiaojiao NIU Yujia LI Yuting zhang Aihua JIA Zhanjun WU Mengqiu School of Medicine Southeast UniversityNanjing 210009China department of Nephrology Children’s Hospital of Nanjing Medical UniversityNanjing 210008China Nanjing Key Laboratory of Pediatrics Children’s Hospital of Nanjing Medical UniversityNanjing 210008China Jiangsu Key Laboratory of Early Development and Chronic Diseases Prevention in Children Nanjing Medical UniversityNanjing 210029China

Pharmaceutical formulations derived from Aristolochiaceae herbs, which contain aristolochic acids(AAs), are widely used for medicinal purposes. However, exposure to these plants and isolated AAs is linked to renal toxicity, known as AA nephropathy(AAN). Currently, the mechanisms underlying AAN are not fully understood, leading to unsatisfactory treatment strategies. In this study, we explored the protective role of 84-B10(5-[[2.(4-methoxyphenoxy)-5-(trifluoromethyl) phenyl] amino]-5-oxo-3-phenylpentanoic acid) against AAN. RNA-seq analysis revealed that the mitochondrion and peroxisome were the most affected cellular components following 84-B10 treatment in AAN mice. Consistently, 84-B10 treatment preserved mitochondrial ultrastructure, restored mitochondrial respiration, enhanced the expression of key transporters(carnitine palmitoyltransferase 2. and enzymes(acylCoenzyme A dehydrogenase, medium chain) involved in mitochondrial fatty acid β-oxidation, and reduced mitochondrial ROS generation in both aristolochic acid I(AAI)-challenged mice kidneys and cultured proximal tubular epithelial cells. Additionally, 84-B10 treatment increased the expression of key transporters(ATP binding cassette subfamily D) and rate-limiting enzymes(acyl-CoA oxidase 1) involved in peroxisomal fatty acid β-oxidation and restored peroxisomal redox balance. Knocking down LONP1 expression diminished the protective effects of 84-B10 against AAN, suggesting LONP1-dependent protection. In conclusion, our study provides evidence that AAN is associated with significant disturbances in both mitochondrial and peroxisomal functions. The LONP1 activator 84-B10 demonstrates therapeutic potential against AAN, likely by maintaining homeostasis in both mitochondria and peroxisomes.

关键词： Aristolochic acid nephropathy 84-B10 LONP1 Mitochondrion Peroxisome

Fluid Flow Behavior of Liquid in Cylindrical Vessels Stirred by One or Two Air Jets

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International Journal of Minerals,Metallurgy and Materials 2000年第3期14卷 170-177页

作者： Lifeng zhang, Shoji Taniguchi, Kaike Cai, Ying Qu ( department of Metallurgy Graduate School of Engineering, Tohoku University, Sendai 980-579 Japan Metallurgy School, University of Science and Technology Beijing, Beijing 100083,{2.) department of Metallurgy Graduate School of Engineering Tohoku University Sendai 980-8579 Japan Metallurgy School University of Science and Technology Beijing Beijing 100083 China

Based on the two-phase model (Eulerian-Eulerian model), the three dimensional fluid flow in water and that liquid steel systems stirred by one or two multiple gas jets are simulated. In the Eulerian-Eulerian two-phase model, the gas and the liquid phase are considered to be two different continuous fluids interacting with each other through the finite inter-phase areas. The exchange between the phases is represented by source terms in conversation equations. Turbulence is assumed to be a property of the liquid phase. A new turbulence modification - model is introduced to consider the bubbles movement contribution to and . The dispersion of phases due to turbulence is represented by introducing a diffusion term in mass conservation equation. The mathematical simulation agrees well with the experiment results. The study results indicate that the distance of two nozzles has big effect on fluid flow behavior in the vessel. Using two gas injection nozzles at the half radii of one diameter of the bottom generates a much better mixing than with one nozzle under the condition of the same total gas flow rate.

关键词： fluid flow two-phase Eulerian-Eulerian model model mathematical simulation

构建“合成生物学”课程群培养面向生物制造的复合型创新人才

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生物工程学报 2024年第9期40卷 3296-3304页

作者：谭高翼张立新张敬宇王启要吴辉庄英萍欧阳立明朱国良王馨叶刘雪婷华东理工大学生物工程学院上海200237

合成生物学(Synthetic Biology)作为一门新兴学科,广受关注且发展迅速,深刻影响着整个生命科学及工程技术领域的发展。与此同时,随着新工科建设的发展,加快培养复合型创新人才是对我国高等教育提出的新任务和新使命。在合成生物学蓬勃... 详细信息

合成生物学(Synthetic Biology)作为一门新兴学科,广受关注且发展迅速,深刻影响着整个生命科学及工程技术领域的发展。与此同时,随着新工科建设的发展,加快培养复合型创新人才是对我国高等教育提出的新任务和新使命。在合成生物学蓬勃发展的背景下,华东理工大学组建了以微生物药物发现和生物制造为核心内容的“合成生物学课程群”。授课团队首先对合成生物学相关的上下游课程体系进行了梳理,随后在原有课程基础上,扩充了合成生物学系列核心课程,课程群不仅涵盖了合成生物学的学科理论和前沿技术,同时锚定了上下游学科。此外,课程群还依托国家重点实验室的国内外名师讲座,并利用学科创新引智基地(“111计划”)雄厚的海外教授资源,致力于提高学生的创新能力。在课程群及授课团队的助力下,学生不仅积极参加合成生物学国际基因工程机器大赛(internationalgeneticengineering machine competition,iGEM)并连续获奖,而且有多人申请专利并参与发表科研论文等。组建合成生物学课程群为培养具有创新能力的复合型人才提供一定的示范。

关键词：合成生物学课程群复合型创新人才国际基因工程机器大赛