Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells
Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells作者机构：Department of Pharmacology Pharmacy College Jinan University Guangzhou China Department of Pathology Medical College Jinan University Guangzhou China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2001年第11卷第4期
页 面：311-315页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Heparin agarose gel electrophoresis flow cytometry apoptosis protein kinase C(PKC)
摘 要：In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopha- ryngeal carcinoma cell line CNE2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc,bax, bcl-2 proteins by use of Hoechst 33258 staining, terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL), agarose gel electrophoresis, and flow cytometry, as well as Western blot analysis. The results showed that heparin induced apoptosis of cNE2 cens including the morphologic changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the ladder pattern revealed by agarose gel electrophoresis of DNA in a concentration-dependent manner. The number of TUNEL-positive cells was dramatically increased to 33.6+1.2% from 2.8+0.3% by treat- ment with heparin in different concentrations (10~40 kU/L). The apoptotic index was increased to 32.5% from 3.5% by detecting SubG1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2, bax and c-myc were significantly overexpressed by treatment with the increase of heparin concentrations. These results suggest that heparin induces apoptosis of CNE2 cells, which may be regulated by differential expression of apoptosis-related genes.
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