检索结果-南通市图书馆

Characteristics of atmospheric oscillation in the Prydz Bay region during the ozone hole period in 1993

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1997年第10期42卷 842-845页

作者： Lu, LH Zhou, XJ Bian, LG zhang, xq Zheng, XD Chinese Academy of Meteorological Sciences Beijing China

IN ref.[1],a preliminary analysis was reported on the change characteristics of total ozoneduring the Antarctic'ozone hole'period in 1993,revealing that there is a very obvious dailychange of total ozone over Zhongshan Station and suggesting that this change would be relatedto the position of Zhongshan Station on the rim of the ozone hole influenced by the polar vortexvacillation and the expanding and contracting processes in Antarctic *** this note,

关键词： Antarctic Prvdz Bay ozone hole atmospheric oscillation.

Vitrificational cryopreservation and subsequently fertile plant regeneration from rice (Oryza sativa L.) embryogenic suspension cells

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1997年第5期42卷 422-426页

作者： Wang, JH Zheng, Y Yan, QF Yan, QS zhang, xq Huang, CN College of Life Science Hangzhou University Hangzhou China China National Rice Research Institute Hangzhou China

CRYOPRESERVATION by vitrification is a new ultralow-temperature-storage technique developed in the 1980s. Operationally, procedures for vitrification of biological specimens consist of the following basic steps: treatment of the samples in a highly concentrated vitrification solution and then plunging the specimens into liquid nitrogen. The word 'vitrification' is sometimes used to describe the high moisture of the in vitro cultures or regenerants. However, in this note, this word means a biophysical process in which the cells and the solution are transformed into a homogeneous and amorphous state. The water in cells does not change into ice, but in

关键词： rice embryogenic suspension cells vitrification cryopreservation plant regeneration.

SYNTHESIS　AND　ANTI－HUMAN　IMMUNODEFICIENCY　VIRUS－1（HIV－1）　ACTIVITY　OF　9－（2－PHOSPHONYLMETHOXYETHYL）ADENINE　（1）AND　ITS　REGIOISOMER　3－（2－PHOSPHONYLMETHOXYETHYL）ADENINE（2）

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1995年第8期6卷 669-672页

作者： Liu, XH Wang, L Guan, BH Kong, M zhang, xq Tao, PZ Chen, HS Chinese Acad Med Sci Inst Mat Med Beijing 100050 Peoples R China Beijing Union Med Coll Beijing 100050 Peoples R China Chinese Acad Med Sci Inst Med Biotechnol Beijing 100050 Peoples R China

We have synthesized the title compounds(1)and(2).The structure of regioisomer(4).a new acyclic nucleotide,was determined by spectroscopic methods (1H-NMR.13C-NMR.13C-1H HMBC).A by-product(7)was also isolated and ident...

关键词：

Effects of 9-cis retinoic acid on human homeobox gene NKX3.1 expression in prostate cancer cell line LNCaP

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2006年第4期8卷 435-441,515页

作者： Jiang, AL zhang, PJ Chen, WW Liu, WW Yu, CX Hu, XY zhang, xq zhang, JY Shandong Univ Sch Med Dept Biochem Jinan 250012 Peoples R China

Aim:To study the regulatory effects of 9-cis retinoic acid(RA)on the expression of human homeobox gene NKX3.1 in prostate cancer cell line ***:Flow cytometry,reverse transcriptase polymerase chain reaction and Western blotting were performed to evaluate the effects of 9-cis RA on NKX3.1 expression and cell cycle of LNCaP *** identify a regulatory region within the NKX3.1 promoter contributing to the regulation induced by 9-cis RA, we have constructed an NKX3.1 promoter-reporter plasmid,pGL_3-1040bp,and its 5′-deletion mutants,which were transfected into LNCaP cells with treatment of 9-cis RA in indicated ***:With the treatment of 9-cis RA,the NKX3.1 promoter activity was increased in reporter gene assay and NKX3.1 expression was enhanced at both mRNA and protein levels in LNCaP *** found that the region between -936 and -921 in the upstream of NKX3.1 gene involved the inducible regulation by 9-cis RA *** flow cytometry,9-cis RA treatment caused accumulation of cells in the G_1 phase of the cell cycle and a fewer cells pass through to G_2/***:Our results demonstrated that 9-cis RA as a differentiating agent can arrest prostate cancer cells in G_1 phase and reduce cell mitosis,and upregulate the expression of human homeobox gene NKX3.1,which is thought to play an important role in prostate differentiation and to act as a tumor suppressor gene in the prostate.(Asian J Androl 2006 Jul;8:435-441)

关键词： NKX3.1 gene 9-cis retinoic acid gene expression prostate cancer cell

Proteomic alterations associated with acquired temozolomide resistance in human malignant gliomas:the potential role of prolyl 4-hydroxylase,beta polypeptide

在线全文

学校读者我要写书评

暂无评论

Proteomic alterations associated with acquired temozolomide ...

hVEGF165基因转染后骨髓间充质干细胞蛋白分泌功能及成骨活性的检测

中国抗癌协会神经肿瘤专业委员会第八届学术会议

作者： Leung GKK Sun S Wong TS xq zhang Pu JKS Lui WM Division of Neurosurgery Department of SurgeryLi Ka Shing Faculty of MedicineThe University of Hong KongQueen Mary Hospital

Introduction Temozolomide（TMZ） provides significant benefits in the treatment of malignant glioma but many patients continue to suffer from progressive or recurrent diseases due to their tumours’ intrinsic or acquired resistance to *** employed proteomic profiling to study the global protein expression changes in TMZ-resistant malignant glioma cell-lines,and investigate the potential role of one of the identified protein candidates,prolyl 4hydroxylase,beta polypeptide（P4HB）,that may be involved in the development of acquired *** Human malignant glioma cell-lines（D54 and U87） with acquired TMZ-resistance were first developed,and their biological behaviours were characterized before protein profiling analysis by two dimensional gel electrophoresis（2DE）.Potential protein spot candidates were identified by MALDI-TOF/*** transfection with small interfering RNAs was used to knock down potential candidates to study their biological *** A total of 15 spots were identified as the most highly up-or down-regulated proteins in TMZ-resistant glioma *** these,the protein P4HB was found to be consistently dysregulated in D54 sublines of different levels of TMZ-resistance when compared to their TMZ-sensitive parental and the passage control *** expression of P4HB transcripts and proteins were also confirmed in U87 cells by qPCR and western blotting *** knock-down was found to significantly down-regulate P4HB expression,and increase TMZ-induced *** P4HB is known to play an important role in hypoxia-induced cell *** present study is the first to describe its role in the regulation of apoptosis in TMZ-treated malignant glioma cells and the development of acquired TMZ-resistance.P4HB is a potential candidate for future studies in TMZ-induced cytotoxicity and novel therapeutic strategies for the treatment of TMZ-resistant malignant glioma.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国组织工程研究与临床康复 2007年第46期11卷 9242-9245页

作者：刘伯龄张锡庆福州市第二医院骨科福建省福州市350007 苏州大学附属儿童医院骨科江苏省苏州市215003

目的:血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)在血管再生和骨组织再生过程中起着重要的调节作用。观察hVEGF165基因转染的骨髓间充质干细胞分泌VEGF的功能及转染后细胞的成骨活性。方法:实验于2004-07/2005-12... 详细信息

目的:血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)在血管再生和骨组织再生过程中起着重要的调节作用。观察hVEGF165基因转染的骨髓间充质干细胞分泌VEGF的功能及转染后细胞的成骨活性。方法:实验于2004-07/2005-12在江苏省血液研究所国家重点实验室完成。①体外分离、培养大鼠骨髓间充质干细胞,传代培养后以1μg PcDNA3.1-hVEGF165∶3μL阳离子脂质体Lipofectamine的比例转染,通过免疫组织化学SP方法检测转染后细胞中外源性VEGF的表达。②体外分离、培养大鼠肾微血管内皮细胞,分别加入hVEGF165基因转染48h及1周后细胞上清、空载体转染及未转染组细胞上清,MTT法观察细胞增殖情况,以了解转染后细胞培养上清中VEGF的生物学活性。③测定在正常条件培养和成骨条件培养下,转染后细胞上清中碱性磷酸酶、骨钙素的水平。结果:①hVEGF165基因转染的骨髓间充质干细胞能成功分泌VEGF蛋白。②hVEGF165基因转染48h组大鼠肾微血管内皮细胞A值高于空载体转染及未转染组(P<0.05),但低于hVEGF165基因转染1周组。③成骨条件培养下,基因转染组细胞碱性磷酸酶和骨钙素的分泌量明显高于未转染组(P<0.05);而在正常条件培养下,基因转染组细胞碱性磷酸酶和骨钙素的表达分泌量较低。结论:①采用基因转染技术可将hVEGF165基因转染到骨髓间充质干细胞中并可有效表达具有生物活性的VEGF,且这种生物学活性呈剂量依赖性。②在成骨条件培养下,转染hVEGF165基因后骨髓间充质干细胞的成骨能力增强。

关键词：骨髓间充质干细胞血管内皮细胞生长因子基因转染血管内皮细胞成骨

China Bencao chemical medicine of discovery and invention（一）

在线全文

学校读者我要写书评

暂无评论

China Bencao chemical medicine of discovery and invention（...

兔血管内皮细胞和诱导成骨细胞在可注射纳米材料上的共培养

中国化学会第十届全国天然有机化学学术会议

作者： Zhao SX Ye WC zhang S Gu JH zhang xq Liu JH Yin ZQ Wang H China Pharmaceucal University Jinan University China Akcademy of Chinese Medicinal Sciences （CACMS）

＜正＞本文着重葛洪在古代化学、化学药物中的发现和发明。例如:葛洪总结魏伯阳《周易参同契》记述的"丹砂化为澒（汞）","欲将（汞）制之,黄牙（硫磺）为根",进一步实践,写出了"丹砂烧之成水银（汞）,积变又还成丹砂"的化学反应;由《...

＜正＞本文着重葛洪在古代化学、化学药物中的发现和发明。例如:葛洪总结魏伯阳《周易参同契》记述的"丹砂化为澒（汞）","欲将（汞）制之,黄牙（硫磺）为根",进一步实践,写出了"丹砂烧之成水银（汞）,积变又还成丹砂"的化学反应;由《淮南万毕术》记述的"夜烧雄黄,水虫成列",进一步实践以升华法制备新的化合物"砒霜"。葛洪炼丹以求"仙药"并写出《抱朴

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国组织工程研究与临床康复 2007年第31期11卷 6125-6129页

作者：张亚王晓东王明海冯林尹航张锡庆苏州大学附属儿童医院骨科江苏省苏州市215003

目的:将可注射纳米骨材料与共培养的兔肾血管内皮细胞和兔骨髓间充质干细胞诱导的成骨细胞复合,并构建可注射细胞型纳米组织工程骨,观察它们体外培养的相容性。方法:实验于2003-09/2004-11在苏州大学附属儿童医院完成。①实验材料:取16... 详细信息

目的:将可注射纳米骨材料与共培养的兔肾血管内皮细胞和兔骨髓间充质干细胞诱导的成骨细胞复合,并构建可注射细胞型纳米组织工程骨,观察它们体外培养的相容性。方法:实验于2003-09/2004-11在苏州大学附属儿童医院完成。①实验材料:取16周龄雄性新西兰大耳白兔,体质量1.5kg左右。②实验方法:麻醉后抽取兔骨髓,用淋巴细胞分离液分离出其中的间充质干细胞,在含体积分数为0.15牛血清的RPMI1640液中培养。骨髓间充质干细胞在条件培养基中,7d后可见细胞变为多边形,碱性磷酸酶和Ⅰ型胶原染色阳性,形成钙结节,表现成骨细胞分化特点。采用三步梯度筛网法,获得肾血管球,5g/L胶原酶Ⅳ37℃消化15～20min,离心沉淀获取血管内皮细胞,在含体积分数为0.15小牛血清的M199中培养。③实验评估:免疫组织化学法进行第Ⅷ因子相关抗原鉴定,透射电镜观察细胞浆Weibel-Palade小体,间接免疫荧光法检测CD31、CD34及CD44的表达。将共培养的兔肾血管内皮细胞、成骨细胞与可注射纳米骨材料体外复合培养,进行形态学观察和功能测定。结果:①在一定培养条件下成功诱导兔骨髓间充质干细胞向成骨细胞分化。②培养的血管内皮细胞免疫组织化学法检测第Ⅷ因子相关抗原阳性,透射电镜观察到细胞浆中的Weibel-Palade小体,间接免疫荧光法检测CD31、CD34表达阳性,CD44阴性。③共培养的兔肾血管内皮细胞、成骨细胞在可注射纳米骨材料上生长、增殖良好,细胞活性和碱性磷酸酶活性未受到影响。结论:可注射纳米骨材料具有良好的细胞相容性,可作为骨组织工程理想的可注射载体材料。

关键词：可注射卡拉胶/纳米相羟基磷灰石/胶原复合材料内皮细胞成骨细胞

Induction of mucosal and systemic immune response by single-dose oral immunization with biodegradable microparticles containing DNA encoding HBsAg

在线全文

学校读者我要写书评

暂无评论

第二军医大学学报 2005年第10期26卷 1197-1197页

作者： He XW Wang F Jiang L Li J Liu SK Xiao ZY Jin xq zhang YN He Y Li K Guo YJ Sun SH Department of Medical Genetics College of Basic Medical UniversitySecond Military Medical UniversityShanghai 200433 China