检索结果-南通市图书馆

维普期刊数据库博看期刊

Cytogenetic Mechanism for the Aneuploidy and Mosaicism Foundin Tetraploid Pacific Oyster Crassostrea gigas （Thunberg）

在线全文

学校读者我要写书评

暂无评论

中国海洋大学学报：英文版 2014年第1期13卷 125-131页

作者： zhang, Zhengrui Wang, Xinglian zhang, quanqi Allen, Standish College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China Qingdao P. R. China Aquaculture Genetics and Breeding Technology Center Virginia Institute of Marine Science College of William and Mary Gloucester Point USA

Chromosome constitution was investigated in adult tetraploid Pacific oyster produced by blocking the first polar body oftriploid eggs which were fertilized with haploid sperms. A high incidence of aneuploid and heteroploid mosaics were found amongthe offspring. Of 20 individuals identified, only 9 （45%） were eutetraploid which contained 40 chromosomes; 2 （10%） were ane-uploid （hypotetraploid）, which contained 39 and 38 chromosomes, respectively; and 9 （45%） were heteroploid mosaics. One mosaicwas consisted of cells containing 40 and 39 chromosomes, respectiovely （1：1 in cell number）, while the other 8 were consisted ofcells containing chromosomes varying between tetraploid and triploid. It was also interesting to note that 3 mosaics even containedsome diploid cells with 20 chromosomes. A certain number of cells of 2 tetraploids and 8 mosaics spread with 32-37 well-scatteredand some clumped chromosomes at metaphase. The percentage of aneuploid cells with chromosomes varying between triploid andtetraploid correlated significantly with that of heteroploid mosaics cells with clumping chromosomes （P〈0.05）. Our findings sug-gested that reversion existed in both tetraploid and triploid oyster and chromosome clumping may underline the chromosome elimi-nation in tetraploid oyster. It seems that the reversing cells, at least some of them, continuously eliminate their chromosomes until themost stable diploid state is established.

关键词： tetraploid oyster aneuploidy mosaicism reversion cytogenetic mechanism

Obstacle-transformer: A trajectory prediction network based on surrounding trajectories

在线全文

学校读者我要写书评

暂无评论

IET Cyber-Systems and Robotics 2023年第1期5卷 1-8页

作者： Wendong zhang Qingjie Chai quanqi zhang Chengwei Wu Department of Control Science and Engineer Harbin Institute of TechnologyHarbinChina

Recurrent Neural Network,Long Short-Term Memory,and Transformer have made great progress in predicting the trajectories of moving *** the trajectory element with the surrounding scene features has been merged to improve performance,there still exist some problems to be *** is that the time series processing models will increase the inference time with the increase of the number of prediction *** problem is that the features cannot be extracted from the scene's image and point cloud in some ***,an Obstacle-Transformer is proposed to predict trajectory in a constant inference ***‘obstacle’is designed by the surrounding trajectory rather than images or point clouds,making Obstacle-Transformer more applicable in a wider range of *** are conducted on ETH and UCY datasets to verify the performance of our model.

关键词： deep-learning trajectory prediction transformer

Large-Scale Screening of Growth-Related Variants in Chinese Tongue Sole(Cynoglossus semilaevis)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2021年第3期20卷 669-680页

作者： SONG Weihao ZHU He WANG Yujue zhang Kai zhang quanqi HE Yan MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China

Growth is the most valuable economic trait for improving aquaculture fish species,since fast growth can reduce labor cost and make more economic ***,the knowledge about how many and which genes are related to the growth of Chi-nese tongue sole is ***-throughput sequencing screening of variants is a fast,economical and accurate assay to identify genes related to growth in crops,livestock and other aquaculture *** the present study,genome-wide resequencing of 30 female Chinese tongue sole individuals from large and small groups to screen growth trait-related variations(SNPs,InDels)was carried *** total,6545735 SNPs and 1016745 InDels were detected,while 31 genes related to growth traits were *** functions were mainly involved in muscle architecture,post-embryonic development,neurosensory development and hormone ***-thermore,18 of the 31 genes related to growth trait are located on W chromosome,indicating that W chromosome plays an important role in regulating the body size of female tongue *** markers and genes identified in our study can be applied to selective breeding of tongue sole and thus will promote the aquaculture industry and increase economic efficiency.

关键词： Cynoglossus semilaevis growth resequencing SNP indel

Genome-Wide Identification of heat shock protein 10/60 Genes in Japanese Flounder (Paralichthys olivaceus) and Their Regulated Expression After Bacterial Infection

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2021年第4期20卷 886-896页

作者： YAN Weijie QIAO Yingjie LIU Yuxiang LIU Xiumei zhang quanqi WANG Xubo Key Laboratory of Marine Genetics and Breeding Ministry of EducationOcean University of ChinaQingdao 266003China College of Life Sciences Yantai UniversityYantai 264005China

Heat shock proteins 10/60(hsp10/60)are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful *** the chaperone and housekeeping functions,they are also known to be involved in immune response during bacterial *** this study,we identified and annotated 10 hsp10/60 genes through bioinformatic analysis in Japanese flounder(Paralichthys olivaceus).Among them one member of hsp10(hspe)family and nine members of hsp60(hspd)family were *** and selection pressure analysis showed that the hsp10/60 genes were evolutionarily constrained and their function was ***,hsp10/60 genes were involved in different embryonic and larval stages and acted as the sentinel role in an unchallenged *** addition,we also observed the expression patterns of hsp10/60 genes after Edwardsiella tarda infection,for the first time in Japanese *** out of 10 genes were differentially expressed after bacterial challenges,the significantly regulated expressions of flounder hsp10/60 genes after bacterial infections suggested their involvement in immune response in *** results provide valuable information for clarifying the evolutionary relationship,and early insights of the immune functions of hsp10/60 genes in Japanese flounder.

关键词： heat shock protein Hsp10/60 Japanese flounder Edwardsiella tarda immune response

Analysis of Genetic Diversity and Differentiation of Seven Stocks of Litopenaeus vannamei Using Microsatellite Markers

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2014年第4期13卷 647-656页

作者： zhang Kai WANG Weiji LI Weiya zhang quanqi KONG Jie College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding (Ministry of Education) Ocean University of China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources (Ministry of Agriculture) Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Shandong Marine Fishing and Production Management Station

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.

关键词： Litopenaeus vannamei microsatellite introduced stock genetic diversity genetic differentiation

Molecular Characterization, Expression Profiles, and Immunostimulation Responses of TRAF6 and TAK1 in Japanese Flounder(Paralichthys olivaceus)

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2019年第1期18卷 165-176页

作者： ZHAO Haitao CHEN Yan WANG Zhigang zhang quanqi YU Haiyang College of Marine Life Sciences Ocean University of China Key Laboratory of Marine Genetics and BreedingMinistry of Education

Tumor necrosis factor receptor-associated factor 6(TRAF6) and transforming growth factor-β-activated kinase 1(TAK1) are two important adaptor molecules in Toll-like receptor(TLR) signaling pathway. In this study, TRAF6(PoTRAF6) and TAK1(PoTAK1) were cloned and characterized in Japanese flounder(Paralichthys olivaceus). The full-length cDNA sequence of Po TRAF6 is 1953 bp, with an open reading frame(ORF) of 1713 bp encoding a putative protein of 570 amino acids. PoTRAF6 contains one really interesting new gene(RING) domain, two zinc fingers, one coiled-coil region, and one meprin and TRAF homology(MATH) domain, which shows a high similarity to TRAF6 s in other species. The full-length PoTAK1 cDNA sequence is 2086 bp, with an ORF of 1728 bp that encodes a putative protein of 575 amino acids. PoTAK1 contains a conserved serine/threonine protein kinase catalytic domain and a coiled-coil region. The promoter regions of PoTRAF6 and PoTAK1 were also analyzed to predict several potential transcription factor-binding sites. In addition, the expression patterns of these two genes were examined in developmental stages, different tissues, and challenged samples. PoTRAF6 and PoTAK1 were expressed during the whole developmental stages, and the highest expressions were in intestine and heart, respectively. In challenged embryonic cells with LPS, CpG ODN, and poly I:C, the expressions of PoTRAF6 and PoTAK1 were both up-regulated significantly. These results suggest that PoTRAF6 and Po TAK1 play crucial roles in immune responses and may be involved in the developmental process of Japanese flounder.

关键词： TRAF6 TAK1 Paralichthys olivaceus cloning expression promoter analysis immunostimulation

Transcriptome-Wide Identification and Validation of Reference Genes in Black Rockfish(Sebastes schlegelii)

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2021年第3期20卷 654-660页

作者： JIN Chaofan SONG Weihao WANG Mengya QI Jie zhang quanqi HE Yan MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

The quantitative real-time reverse transcription PCR(qRT-PCR)is a widely used technique to analyze gene expression *** a suitable reference gene is a crucial step to obtain an accurate result in ***,most previous studies on fishes adopted reference genes that were commonly used in mammals without *** this study,we utilized 89 transcrip-tome datasets covering early developmental stages and different adult tissues,and carried out transcriptome-wide identification and validation of reference genes in Sebastes ***,121 candidate reference genes were identified based on four *** candidates(METAP2,BTF3L4,EIF5A1,TCTP,UBC,PAIRB,RAB10,and DLD)and four commonly used reference genes in mam-mals(TUBA,ACTB,GAPDH,RPL17)were selected for validation via qRT-PCR and four statistical analysis methods(delta-Ct,Best-Keeper,geNorm,and NormFinder).The results indicated that when the black rockfish are cultured in a general condition,the eight candidate reference genes are more stable than traditional reference genes in mammals,and RAB10,EIF5A1,PAIRB and BTF3L4 are the best reference genes in *** is the first study to conduct transcriptome-wide identification and validation of reference genes for quantitative RT-PCR in the black rockfish,and lay an important foundation for gene expression analysis in teleost.

关键词： reference genes black rockfish transcriptome-wide CV value qRT-PCR

Preferential Bivalent Formation in Tetraploid Male of Pacific Oyster Crassostrea gigas Thunberg

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2014年第2期13卷 297-302页

作者： zhang Zhengrui WANG Xinglian zhang quanqi Standish Allen Jr. College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding of Ministry of EducationOcean University of China Aquaculture Genetics and Breeding Technology Center Virginia Institute of Marine ScienceCollege of William and Mary

Artificially induced tetraploid Pacific oyster, Crassostrea gigas Thunberg, produces more aneuploid gametes than nor- mal diploid one, although they showed a comparable fecundity to diploidy. The meiotic chromosome configuration of 3 tetraploid and 1 tetraploid/triploid mosaic males were analyzed through direct chromosome observation. A majority of metaphase I spermato- cytes contained both bivalents and quadrivalents. The chromosome configuration of these males was characterized by preferential formation of bivalents to quadrivalents. Bivalents appeared in all spermatocytes and consisted of 86% of all chromosome aggregates. In comparison, quadrivalents occurred in 91% spermatocytes and consisted of only 12.6% of all chromosome aggregates. The mean bivalent frequency per spermatocyte varied between 14.4 and 17.2; while that of quadrivalents varied between 2.2 and 2.7. Most quadrivalents were tandemly chained （58%） or circled （39%）. The total number of chromosome aggregates per spermatocyte ranged from 13 to 20 with an average of 17.6; while 18 （16 bivalents and 2 quadrivalents） was the most frequent. Univalents and trivalents appeared in very low frequency. Aneuploid （hypotetraploid） spermatocytes were observed in a low frequency. The chromosome con- figuration of in the mosaic individual was similar to that of tetraploid individuals. The percentage of triploid spermatocytes （2%） of the mosaic individual was significantly lower （2＂2 =30, P〈 0.01） than that of triploid cells （46%） in its somatic tissue.

关键词： tetraploid oyster meiosis bivalent quadrivalent reversion

Identification of differential genes in the ovary relative to the testis and their expression patterns in half-smooth tongue sole (Cynoglossus semilaevis)

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第2期37卷 137-145页

作者： Yeying Sun quanqi zhang Jie Qi Yanjie Chen Qiwang Zhong Chunmei Li Key Laboratory of Marine Genetics and Breeding (College of Marine Life Science Ocean University of China) Ministry of Education Qingdao 266003 China College of Pharmacy Binzhou Medical University Yantai 264003 China

Half-smooth tongue sole （Cynoglossus semilaevis） is a rare marine flatfish whose mature ovary and testis greatly differ in volume and weight. The length and weight of mature females are over twice greater than those of mature males. To obtain sufficient information on gonad differentiation and the relationship between gonad development and growth in the fish, we compared gene expression between the ovary and testis using suppression subtractive hybridization （SSH）. Testis cDNAs are subtracted from ovary cDNAs and are used to es- tablish an ovary testis-subtracted cDNA library. A total of 41 nonredundant clones are identified, including 20 known cDNAs, 9 unchar-acterized cDNAs （EST clones）, and 12 novel sequences. For selected genes such as ZPC, RacGAP, survivin, aquaporin, CPEB, O5, O15, and O18, gene expression patterns are analyzed by reverse transcriptase-polymerase chain reaction （RT-PCR）. The results confirm that these genes are only expressed in the ovary and not in the testis, or at higher levels in the ovary than in the testis. At the same time, expressions of certain genes such as ZPC, survivin, aquaporin, CPEB, and O15 are demonstrated to possess sexual dimorphism in the kidney or muscle, and spleen. The results suggest that these genes could play key roles not only in the ovary but in other female tissues as well.

关键词： Cynoglossus semilaevis SSH gene expression sexual dimorphism RT-PCR