Analysis of Genetic Diversity and Differentiation of Seven Stocks of Litopenaeus vannamei Using Microsatellite Markers

作     者：ZHANG Kai WANG Weiji LI Weiya ZHANG Quanqi KONG Jie 

作者机构：College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding (Ministry of Education) Ocean University of China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources (Ministry of Agriculture) Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Shandong Marine Fishing and Production Management Station 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2014年第13卷第4期

页      面：647-656页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 0707[理学-海洋科学] 09[农学] 0815[工学-水利工程] 0824[工学-船舶与海洋工程] 

基　　金：supported by the National High Technology Development Project of China under contract No. 2012AA10A404 the Recommended International Advanced Agricultural Science and Technology Project of China under contract No. 2012-S5 

主　　题：Litopenaeus vannamei microsatellite introduced stock genetic diversity genetic differentiation 

摘      要：Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.