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典型固体废弃物选择性破碎技术研究进展

环境工程 2019年第6期37卷 141-145页

作者：黄庆张承龙王景伟上海第二工业大学资源循环科学与工程中心上海201209 上海第二工业大学上海电子废弃物资源化协同创新中心上海201209

综述了选择性破碎技术在典型固体废弃物(城市活垃圾、煤矸石、废弃电子电器产品)领域的应用进展,并介绍了破碎功耗学说和选择性破碎功耗理论的研究进展,强调破碎过程中的能量消耗与物料特性、设备结构等因素相关,进一步说明选择性破碎... 详细信息

综述了选择性破碎技术在典型固体废弃物(城市活垃圾、煤矸石、废弃电子电器产品)领域的应用进展,并介绍了破碎功耗学说和选择性破碎功耗理论的研究进展,强调破碎过程中的能量消耗与物料特性、设备结构等因素相关,进一步说明选择性破碎在能耗降低和破碎效率等方面有显著优势,从而为废弃物再生利用和资源化提供一条可节能降耗的新思路。

关键词：选择性破碎资源化煤矸石废弃电子电器产品功耗

法螺G型溶菌酶重组蛋白的原核表达及抑菌活性探究

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海洋科学 2022年第10期46卷 32-42页

作者：贾慧霞张华张成龙何毛贤刘文广中国科学院热带海洋生物资源与生态重点实验室广东省应用海洋生物学重点实验室中国科学院南海海洋研究所广东广州510301 中国科学院大学北京100049 南方海洋科学与工程广东省实验室(广州) 广东广州511458 三沙市海洋和渔业局海南三沙573199

G型溶菌酶(G-type lysozyme,***)是一种富含半胱氨酸的天然免疫因子,在无脊椎动物的免疫防御过程中起重要作用。本研究旨在通过揭示法螺(Charonia tritonis)G型溶菌酶(***)重组蛋白的抑菌功能,为进一步认识法螺的分子免疫机理及病害防... 详细信息

G型溶菌酶(G-type lysozyme,***)是一种富含半胱氨酸的天然免疫因子,在无脊椎动物的免疫防御过程中起重要作用。本研究旨在通过揭示法螺(Charonia tritonis)G型溶菌酶(***)重组蛋白的抑菌功能,为进一步认识法螺的分子免疫机理及病害防治提供新思路。首先对***序列进行了分析,利用实时荧光定量PCR检测***的组织分布,然后以pGEX-4T-1为表达载体,构建重组质粒***,进行重组蛋白表达,并检测了重组蛋白的抑菌活性。结果显示:1)*** cDNA的5′非翻译区(UTR)为66 bp,3′UTR为186 bp,开放阅读框为789 bp,编码262个氨基酸,有1个可溶性转糖苷酶(SLT)结构域(72~255 aa),包含6个半胱氨酸残基和3个酶活性催化位点(Glu72、Asp85、Asp96);2)多序列比对及系统发育分析表明,***与亲缘关系较近的福寿螺G型溶菌酶氨基酸的同源性最高;3)***在所有被检测组织中均表达,其中在肝脏、外套膜表达量较高;4)16℃,0.5 mmol/L IPTG诱导表达10 h后,重组蛋白在上清液和包涵体均表达分子量约为51.91 kDa,纯化后得到浓度为1.2 mg/mL的重组蛋白;5)抑菌实验表明,重组蛋白***具有抗金黄色葡萄球菌Staphyloccocus aureus、藤黄微球菌Micrococcus luteus、施罗氏弧菌Vibrio shilonii的活性,对革兰氏阳性细菌的抑菌作用大于革兰氏阴性细菌。研究结果为进一步解析法螺免疫防御的分子机理提供了参考数据和科学依据。

关键词：法螺 G型溶菌酶原核表达抑菌活性

Enhanced rate capability and cycle stability of Ti2C MXene for sodium storage through an aniline molecules welding strategy

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Tungsten 2025年第1期7卷 161-171页

作者： Hao Chen Ya-Qin Wang Dong-Ting zhang Bei Zhao Min-Peng Li Chen-Yang Li Tian-Peng Xu Mao-cheng Liu State Key Laboratory of Advanced Processing and Recycling of Non-Ferrous Metals Lanzhou University of Technology School of Materials Science and Engineering Lanzhou University of Technology School of Computer and Communication Technology Lanzhou University of Technology

MXenes obtained significant attention in the field of energy storage devices due to their characteristic layered structure,modifiable surface functional groups,large electrochemically active surface,and regulable interlayer ***,the self-restacking and sluggish ions diffusion kinetics performance of MXenes during the alkali metal ions insertion/extraction process severely impedes their cycle stability and rate *** paper proposes an aniline molecule welding strategy for welding p-phenylenediamine（PPDA） into the interlayers of Ti2C through a dehydration condensation *** welded PPDA molecules can contribute pillar effect to the layered structure of *** pillar effect effectively maintains the structural stability during the sodium ions insertion/extraction process and effectively expands the interlayer spacing of Ti2C from 1.16 to 1.38 nm,thereby enhancing ions diffusion kinetics performance and improving the long-term cycle *** Ti2C-PPDA demonstrates outstanding Na+storage capability,exhibiting a specific capacity of 100.2 mAh·g-1at a current density of 0.1 A·g-1over 960 cycles and delivering a remarkable rate capability 81.2 mAh·g-1at a current density of 5 A·*** study demonstrates that expanding interlayer spacing is a promising strategy to enhance the Na+storage capacity and improve long-term cycling stability,which provides significant guidance for the design of two-dimensional Na+storage materials with high-rate capability and cycle stability.

关键词： Diffusion kinetics Expanding interlayer spacing Rate capability Sodium ions storage Ti2C

Ni-catalyzed cross-electrophile alkyl-alkyl coupling reactions

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Science China Chemistry 2025年

作者： Li-Xu You Lan Tian Chun-Ling Guo Shun-Xi Li Yu-cheng Liu Yu-long Li Wei Shu College of Chemistry and Environmental Engineering Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan Sichuan University of Science and Engineering Guangming Advanced Research Institute Shenzhen Grubbs Institute Department of Chemistry and Guangdong Provincial Key Laboratory of Catalysis Southern University of Science and Technology

Introducing saturated carbon-carbon bonds into organic molecules significantly increases the three-dimensional configuration,thus altering the physical and chemical properties as well as the functions of molecules. Transition-metal-catalyzed carboncarbon cross-couplings represent one of the most straightforward, efficient and modular alternatives to build saturation in a single step. However, the efficient construction of alkyl-alkyl bonds remains a formidable challenge due to the precise manipulation of multiple alkyl metallic intermediates as well as associated competitive side reactions and selectivity control. Over the past years, Ni-catalyzed cross-coupling under reductive conditions has gained more and more attention for alkyl-alkyl crosscoupling partially attributing to the low-valent state of metal centers under reducing conditions, which suppresses the competitive β-hydride elimination process, thus facilitating the formation of alkyl-alkyl bonds. In this review, the initiative and progress of Ni-catalyzed cross-electrophile alkyl-alkyl cross-coupling, including the efforts in racemic and enantioselective formation of alkyl-alkyl bonds, have been summarized and discussed. Moreover, the challenges and further opportunities in this area have also been discussed.

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云雾山草原不同深度土壤的呼吸特征及其对温度变化的响应

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草业科学 2017年第2期34卷 224-230页

作者：张浩叶成龙王益郭辉胡水金生态系统生态学实验室南京农业大学资源与环境科学学院江苏南京210095 黄土与第四纪地质国家重点实验室中国科学院地球环境研究所陕西西安710061

土壤呼吸及其对温度的敏感性是预测碳循环过程的重要参数,近年来受到了广泛关注。但在黄土高原地区,不同深度土壤微生物呼吸特征及其对温度变化响应的相关研究尚未见报道。本研究选取黄土高原云雾山草原自然保护区围封30年的典型草地作... 详细信息

土壤呼吸及其对温度的敏感性是预测碳循环过程的重要参数,近年来受到了广泛关注。但在黄土高原地区,不同深度土壤微生物呼吸特征及其对温度变化响应的相关研究尚未见报道。本研究选取黄土高原云雾山草原自然保护区围封30年的典型草地作为研究地点,采集了不同深度的土壤样品(0-10、10-20、20-30、30-40、40-50、50-60cm),在室内25和35℃条件下对土壤样品进行培养,探讨不同深度土层的土壤呼吸及其对温度敏感性的变化。结果表明,土壤有机碳、全氮、有效氮、速效磷,全磷及微生物生物量碳氮含量均随土层深度的增加而显著降低(P<0.05)。随着培养时间和土层深度的增加,两个温度培养下的土壤呼吸速率和土壤呼吸累积碳释放量均显著下降(P<0.05)。然而,随着土层深度增加,土壤呼吸温度敏感性呈上升趋势。冗余分析表明,土壤呼吸速率与土壤性质均有较好的正相关,而土壤呼吸温度敏感性与各土壤因子呈负相关。

关键词：黄土高原深层土壤微生物呼吸温度敏感性

Infiltration by monocytes of the central nervous system and its role in multiple sclerosis: reflections on therapeutic strategies

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Neural Regeneration Research 2025年第3期20卷 779-793页

作者： Guangyong zhang Qing Yao Chubing long Pengcheng Yi Jiali Song Luojia Wu Wei Wan Xiuqin Rao Yue Lin Gen Wei Jun Ying Fuzhou Hua Department of Anesthesiology the Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Key Laboratory of Anesthesiology of Jiangxi Province NanchangJiangxi ProvinceChina

Mononuclear macrophage infiltration in the central nervous system is a prominent feature of neuroinflammation. Recent studies on the pathogenesis and progression of multiple sclerosis have highlighted the multiple roles of mononuclear macrophages in the neuroinflammatory process. Monocytes play a significant role in neuroinflammation, and managing neuroinflammation by manipulating peripheral monocytes stands out as an effective strategy for the treatment of multiple sclerosis, leading to improved patient outcomes. This review outlines the steps involved in the entry of myeloid monocytes into the central nervous system that are targets for effective intervention: the activation of bone marrow hematopoiesis, migration of monocytes in the blood, and penetration of the blood–brain barrier by monocytes. Finally, we summarize the different monocyte subpopulations and their effects on the central nervous system based on phenotypic differences. As activated microglia resemble monocyte-derived macrophages, it is important to accurately identify the role of monocyte-derived macrophages in disease. Depending on the roles played by monocyte-derived macrophages at different stages of the disease, several of these processes can be interrupted to limit neuroinflammation and improve patient prognosis. Here, we discuss possible strategies to target monocytes in neurological diseases, focusing on three key aspects of monocyte infiltration into the central nervous system, to provide new ideas for the treatment of neurodegenerative diseases.

关键词： blood–brain barrier macrophages monocytes multiple sclerosis neuroinflammation review therapy

面向离散制造物联车间的UWB实时定位系统设计

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组合机床与自动化加工技术 2017年第6期 86-89页

作者：张成龙李少波陈伟兴贵州大学大数据与信息工程学院贵阳550025 贵州大学机械工程学院贵阳550025 贵州大学现代制造技术教育部重点实验室贵阳550025

离散型制造业生产过程复杂多变,制造资源具有更为广泛的动态特性,为进行有效的制造资源管理控制,实现制造物联车间实时位置数据的采集,基于制造物联事件主动感知技术架构,提出了一种离散制造物联车间资源定位管理模型,设计了一种基于超... 详细信息

离散型制造业生产过程复杂多变,制造资源具有更为广泛的动态特性,为进行有效的制造资源管理控制,实现制造物联车间实时位置数据的采集,基于制造物联事件主动感知技术架构,提出了一种离散制造物联车间资源定位管理模型,设计了一种基于超宽带技术的制造资源定位系统,改进了一种基于自适应阈值函数的小波阈值去噪算法并实现,能够有效降低外界因素的干扰,减少定位误差,实现对离散制造车间制造资源的实时定位,有效获取人员、物料、物流车辆等制造资源的位置数据,便于进行资源感知和调度管理,从而提升管理标准及产品品质。

关键词：超宽带技术实时定位离散型制造业制造物联信息系统

Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala:association with enhanced cued fear expression

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Neural Regeneration Research 2025年第11期20卷 3259-3272页

作者： Huidong Li Faqin Li Zhaoyi Chen Erwen Wu Xiaoxi Dai Danni Li Haojie An Shiyi Zeng Chunyan Wang Li Yang cheng long School of Life Sciences South China Normal UniversityGuangzhouGuangdong ProvinceChina School of Life Sciences Guangzhou UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Major Obstetric Diseases The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research Panyu Central HospitalGuangzhouGuangdong ProvinceChina

Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear ***(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B *** is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly *** studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like ***,the precise role of CYLD in glutamatergic neurons is largely ***,we first proposed involvement of CYLD in cued fear *** next constructed transgenic model mice with specific deletion of Cyld from glutamatergic *** results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male ***,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male ***,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial *** may contribute,at least in part,to cued fear expression.

关键词： basolateral amygdala cued fear expression cylindromatosis deubiquitinase glutamate receptor 1 glutamatergic neuron microglial activation N-methyl-D-aspartate receptor 1 neuronal activation synaptic transmission

右归饮通过Wnt/ERK信号通路交叉串扰影响成骨细胞相关蛋白的表达

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中华中医药杂志 2016年第10期31卷 4249-4252页

作者：范连霞章建华尹华张成龙浙江中医药大学杭州310053 浙江中医药大学附属第一医院杭州310053

目的:观察细胞外信号调节激酶(ERK)、Wnt/β-连环蛋白(β-catenin)信号通路相关蛋白在右归饮含药血清影响成骨细胞过程中的作用,明确右归饮含药血清对ERK、Wnt通路和ERK/Wnt交叉串扰的作用。方法:体外培养成骨细胞,分组,加药干预。培养3... 详细信息

目的:观察细胞外信号调节激酶(ERK)、Wnt/β-连环蛋白(β-catenin)信号通路相关蛋白在右归饮含药血清影响成骨细胞过程中的作用,明确右归饮含药血清对ERK、Wnt通路和ERK/Wnt交叉串扰的作用。方法:体外培养成骨细胞,分组,加药干预。培养3d后,应用ELISA检测Wnt/β-catenin及ERK通路相关蛋白β-catenin、Runx2、ERK蛋白表达情况。结果:右归饮含药血清能显著上调3种蛋白表达(P<0.05,P<0.01)。联合阻断ERK、Wnt通路,3种蛋白表达水平较含药血清组有明显降低(P<0.05,P<0.01);与单独阻断其中一条通路比较,有明显下降(P<0.01)。单独阻断某一条通路,仅Runx2较右归饮含药血清组有明显降低(P<0.05,P<0.01)。结论:右归饮含药血清能显著增加成骨细胞活性,该作用与ERK、Wnt信号通路密切相关,且两条通路之间存在一定的交叉串扰作用。

关键词： Wnt/β-catenin信号通路 ERK信号通路交叉串扰右归饮成骨细胞