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Seeking for endometrial carcinoma-related genes and their bioinformatics analysis

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广西医科大学学报 2017年第7期34卷 961-966页

作者： Yicheng Su jieqing zhang Department of Gynecologic Oncology the Affiliated Tumor Hospital of Guangxi Medical University Nanning 530021China

Objective:Screening and excavating genes and biological information related to endometrial carcinoma(EC),to provide novel means for clinical diagnosis and ***:The gene chip data of EC was obtained from the Gene Expression Omnibus(GEO)database,we integrated gene discrepantly expressive gene analysis,enrichment of genic function and pathway analysis,protein-protein interaction(PPI)network and literature mining to predict genes and pathways of ***:Functional enrichment analysis identified 976differential expression genes were participated in cell cycle regulation,proliferation,biosynthesis,protein phosphorylation and fission biological process,*** found eight significantly up-regulated pathways in the microarray datasets,including the MAPK signaling pathway and mTOR pathway,and six down-regulated pathways,including the P53signaling pathway and cell *** protein related interaction network analysis was further performed,and we found twelve distinct genes,including the PCNA and CCND1,these genes were located in key position of EC related network,which may be related to the pathogenesis and progression of ***,there was a high correlation between PCNA and CCND1with EC by consulting the related literature and data,however,the functional mechanism was ***:The pathogenesis and progression of EC may involve in several different pathways and genes,the genes PCNA,CCND1and MAPK signaling pathway may be valuable.

关键词： endometrial carcinoma gene chip GEO bioin-formatics analysis

Apparent Stress Changes of the 2010 Luanxian Earthquake Sequence

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Earthquake Research in China 2015年第3期29卷 293-302页

作者： Li He Liu Wenbing Wang Yixi zhang jieqing Gao Ye Kang Jian zhang Changxuan Earthquake Administration of Tianjin Municipality Institute of Geophysics CEA

On March 6,2010,an earthquake of M L4. 5 took place in Luanxian,Hebei Province,with plenty of foreshocks and aftershocks. From December 2009 to March 2010,a series of M L≥ 2. 5 earthquakes were recorded by the digital seismic network of the capital region,which were selected to calculate the apparent stress in this region. The results show that,firstly,a high value anomaly of apparent stress appeared before the M L4. 5 and peak value appeared on the main shock, which then decreased after the ML4. 5 earthquake. The apparent stress of the main shock is much greater than that of most aftershocks,the sequence type is considered as a main shock-aftershock. Secondly,the size of apparent stress perfectly reflects the state of the stress field in the hypocenter region,and we can discuss seismic sequence properties through the changing process of apparent stress,in combination with the traditional methods to identify a sequence more accurately. Finally,in the case of magnitude less than or equal to M L3. 3,correlation between magnitude and apparent stress is positive.

关键词： Apparent stress Focal mechanism solution Luanxian earthquake sequence

亚利桑德罗·伊纳里图：世界与民族的影像叙事

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电影评介 2019年第15期 63-66页

作者：张洁清杨希河北传媒学院

亚利桑德罗·伊纳里图是墨西哥电影三杰之一,从20世纪90年代早期开始尝试拍摄反映墨西哥生活的短片到2015年的《荒野猎人》,他已凭借多部杰出的作品获得过多次国际荣誉。2000年,亚利桑德罗·伊纳里图的处女作《爱情是狗娘》横空出世,这... 详细信息

亚利桑德罗·伊纳里图是墨西哥电影三杰之一,从20世纪90年代早期开始尝试拍摄反映墨西哥生活的短片到2015年的《荒野猎人》,他已凭借多部杰出的作品获得过多次国际荣誉。2000年,亚利桑德罗·伊纳里图的处女作《爱情是狗娘》横空出世,这部电影大受好评,由此确立了这位墨西哥导演的制作班底。墨西哥本土独特的艺术气质、表达方式同后全球化时代里普世性的现代主题在其日后的《21克》《通天塔》《鸟人》等电影中一直水乳交融,不可分割地延续着。他所讲述的不仅仅是一个世界中的墨西哥,更还是墨西哥中的世界。

关键词：《荒野猎人》《鸟人》艺术气质《21克》《通天塔》影像叙事墨西哥电影伊纳

Prostate specific membrane antigen knockdown impairs the tumorigenicity of LNCaP prostate cancer cells by inhibiting the phosphatidylinositol 3-kinase/Akt signaling pathway

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Chinese Medical Journal 2014年第5期127卷 929-936页

作者： Guo Zhenghui Lai Yiming Du Tao zhang Yiming Chen jieqing Bi Liangkuan Lin Tianxin Liu Hao Wang Wei Xu Kewei Jiang Chun Han Jinli zhang Caixia Dong Wen Huang Jian Huang Hai Department of Urology Sun Yat-sen Memorial Hospital Sun Yat-sen University Guangzhou Guangdong 510120 China

Background Prostate specific membrane antigen （PSMA） can facilitate the growth,migration,and invasion of the LNCaP prostate cancer cell lines,but the underlying molecular mechanisms have not yet been clearly ***,we investigated whether PSMA serves as a novel regulator of the phosphatidylinositol 3-kinase （PI3K）/Akt signaling by employing PSMA knockdown model and PI3K pharmacological inhibitor （LY294002） in LNCaP prostate cancer *** PSMA knockdown had been stably established by transfecting with lentivirus-mediated siRNA in our previous ***,LNCaP cells were divided into interference,non-interference,and blank *** first testified the efficacy of PSMA knockdown in our LNCaP cell ***,we compared the expression of PSMA and total/activated Akt by Westem blotting in the above three groups with or without LY294002 ***,immunocytochemistry was performed to confirm the changes of activated Akt （p-Akt,Ser473） in ***,cell proliferation,migration,and cell cycle were measured by CCK-8 assay,Transwell analysis,and Flow cytometry *** After PSMA knockdown,the level of p-Akt （Ser473） but not of total-Akt （Akt1/2） was significantly decreased when compared with the non-interference and blank ***,LY294002 administration significantly reduced the expression of p-Akt （Ser473） in all the three *** results of immunocytochemistry further confirmed that PSMA knockdown or LY294002 treatment was associated with p-Akt （Ser473） *** of cell proliferation,migration,and survival were also observed upon PSMA knockdown and LY294002 *** Taken together,our results reveal that PI3K/Akt signaling pathway inhibition may serve as a novel molecular mechanism in LNCaP prostate cancer cells of PSMA knockdown and suggest that Akt （Ser473） may play a critical role as a downstream signaling target effector of PSMA in this cellular model.

关键词： prostate cancer LNCaP cells prostate-specific membrane antigen RNA interference PI3K/Akt pathway tumorigenicity

基于GoogLeNet和双层GRU的图像描述

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陕西师范大学学报（自然科学版） 2021年第1期49卷 68-73页

作者：张洁庆郭敏肖冰陕西师范大学计算机科学学院陕西西安710119

针对基于卷积神经网络(convolutional neural network,CNN)和长短期记忆网络(long short-term memory,LSTM)的方法存在计算复杂度高、收敛速度慢、训练时间长等问题,本文提出基于GoogLeNet和双层GRU的图像描述模型,在训练阶段采用适应... 详细信息

针对基于卷积神经网络(convolutional neural network,CNN)和长短期记忆网络(long short-term memory,LSTM)的方法存在计算复杂度高、收敛速度慢、训练时间长等问题,本文提出基于GoogLeNet和双层GRU的图像描述模型,在训练阶段采用适应性动量估计法(adaptive moment estimation,Adam)优化算法,加快了整体模型的收敛速率,提高了模型性能。在MSCOCO和Flickr30K两个数据集上的实验结果表明,基于GoogLeNet和双层GRU的图像描述模型实验效果优于目前常用的图像描述模型,生成的句子准确度更高,在多个评价指标上超过了其他常用图像描述模型。

关键词：图像描述 GoogLeNet 门限递归单元适应性动量估计法

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基坑地下承压水的控制设计及抢险方法

岩石力学与工程学报 2000年第Z1期19卷 1108-1110页

作者：张杰青陈海兵施木俊唐传政武汉市勘测设计研究院武汉430022 广东顺德市水电公司顺德528300

结合工程实例 ,总结了基坑地下承压水控制设计及抢险方法。

关键词：基坑,承压水,控制设计,深井降水,抢险

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影响合成氨综合能耗主要因素的分析

大氮肥 2002年第2期25卷 116-118页

作者：张杰卿镇海炼化股份有限公司化肥厂浙江宁波315207

合成氨综合能耗是化肥生产企业一项重要的技术经济指标。结合镇海炼化股份有限公司化肥厂生产实际 ,对影响合成氨综合能耗的主要因素分别进行分析。

关键词：合成氨综合能耗影响因素回归分析

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基坑工程概念设计中的辨证思考

城市勘测 2010年第1期 169-171页

作者：张杰青武汉市勘测设计研究院湖北武汉430022

对基坑工程支护设计中临时与永久、顺作法与逆作法,地下水治理堵与疏、静态设计与动态设计等4个方面进行了论述,以期加大对基坑工程概念设计的辨证认识。

关键词：基坑工程概念设计辨证思考

沉默PI3K基因对E_(2)刺激后子宫内膜癌细胞增殖和凋亡的影响

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现代妇产科进展 2021年第4期30卷 270-272页

作者：陈坤张洁清李力广西医科大学附属肿瘤医院妇瘤科南宁530000

目的:探讨沉默PI3K基因对雌激素刺激下的子宫内膜癌细胞增殖、迁移、凋亡的影响。方法:培养子宫内膜癌Ishikawa细胞,构建LV-PI3K-RNAi慢病毒载体转染Ishikawa细胞。实验分组Control组未经任何处理的Ishikawa细胞;Ishikawa组经E_(2)刺激... 详细信息

目的:探讨沉默PI3K基因对雌激素刺激下的子宫内膜癌细胞增殖、迁移、凋亡的影响。方法:培养子宫内膜癌Ishikawa细胞,构建LV-PI3K-RNAi慢病毒载体转染Ishikawa细胞。实验分组Control组未经任何处理的Ishikawa细胞;Ishikawa组经E_(2)刺激的Ishikawa细胞;NC组经E_(2)刺激并转染阴性对照慢病毒的Ishikawa细胞;PI3Ki组经E_(2)刺激并转染LV-PI3K-RNAi慢病毒的Ishikawa细胞。Real-time PCR、Western blot法检测各组VEGF、bFGF、PI3K mRNA及蛋白表达水平,MTT法、流式细胞仪分别检测细胞增殖能力及凋亡的情况。结果:与Ishikawa组、NC组比较,PI3Ki组的VEGF、bFGF、PI3K mRNA及蛋白表达水平明显降低,细胞增殖能力显著降低,凋亡率增高,差异均有统计学意义(P<0.05)。结论:PI3K基因低表达可干扰E_(2)在基因、蛋白水平激活Ishikawa细胞产生VEGF、bFGF,从而下调E_(2)对子宫内膜癌细胞增殖和抑制凋亡的影响。

关键词：子宫内膜癌雌二醇 VEGF bFGF RNA干扰 PI3K/Akt