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Innovative 3D Microfluidic Intestinal Organoid Model for Assessing Cadmium Bioavailability in Food: Implications for Enhanced Exposure Risk Assessment

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Food Science and Human Wellness 2024年

作者： Yan Li Wen Sun Qiao Wang Wan Shi Yu Chen zhiyong gong Xiao Guo Xin Liu Yongning Wu Key Laboratory of Animal Biological Products & Genetic Engineering， Ministry of Agriculture and Rural， Sinopharm Animal Health Corporation Ltd. State Key Laboratory of Novel Vaccines for Emerging Infectious Diseases， China National Biotec Group Company Limited Key Laboratory for Deep Processing of Major Grain and Oil (The Chinese Ministry of Education)， College of Food Science and Engineering， Wuhan Polytechnic University Department of Oncology， Xiangya Hospital， Central South University NHC Key Laboratory of Food Safety Risk Assessment， China National Center for Food Safety Risk Assessment

Given the severe toxicity and widespread presence of cadmium (Cd) in staple foods such as rice， accurate dietary exposure assessments are imperative for public health. In vitro bioavailability is commonly used to adjust dietary exposure levels of risk factors; however， traditional planar Transwell models have limitations， such as cell dedifferentiation and lack of key intestinal components， necessitating a more physiologically relevant in vitro platform. This study introduces an innovative three-dimensional (3D) intestinal organoid model using a microfluidic chip to evaluate Cd bioavailability in food. Caco-2 cells were cultured on the chip to mimic small intestinal villi's 3D structure， mucus production， and absorption functions. The model's physiological relevance was thoroughly characterized， demonstrating the formation of a confluent epithelial monolayer with well-developed tight junctions (ZO-1)， high microvilli density (F-actin)， and significant mucus secretion (Alcian blue staining)， closely resembling the physiological intestinal epithelium. Fluorescent particle tracking confirmed its ability to simulate intestinal transport and diffusion. The Cd bioavailability in rice measured by the 3D intestinal organoid model (9.07% ± 0.21%) was comparable to the mouse model (12.82% ± 3.42%) but significantly lower than the Caco-2 monolayer model (26.97% ± 1.11%). This 3D intestinal organoid model provides a novel and reliable strategy for in vitro assessment of heavy metal bioavailability in food， with important implications for food safety and risk assessment.

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迷迭香酸共价偶联对β-乳球蛋白结构及性质的影响

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食品科学 2024年第11期45卷 61-67页

作者：杨庆尚洁丽曾浩龙王璇霈陈义杰柳鑫宫智勇许琳武汉轻工大学大宗粮油精深加工教育部重点实验室湖北武汉430023 华中科技大学同济医学院附属同济医院湖北武汉430030 华中农业大学食品科学技术学院湖北武汉430070

以β-乳球蛋白(β-lactoglobulin,β-LG)为对象,探究膳食多酚迷迭香酸(rosmarinic acid,RA)共价偶联对蛋白结构和性质的影响。采用自由基法和碱法制得β-LG-RA共价偶联复合物;利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、傅里叶变换红外光... 详细信息

以β-乳球蛋白(β-lactoglobulin,β-LG)为对象,探究膳食多酚迷迭香酸(rosmarinic acid,RA)共价偶联对蛋白结构和性质的影响。采用自由基法和碱法制得β-LG-RA共价偶联复合物;利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、傅里叶变换红外光谱、紫外吸收光谱、荧光光谱、圆二色光谱等技术,分析RA共价偶联后β-LG结构变化规律;通过对1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除率检测及酶联免疫吸附测试,评价β-LG与RA共价偶联前后抗氧化性和与血清特异性免疫球蛋白G(immunoglobulin G,IgG)结合能力的变化。结果表明:RA共价偶联后β-LG的二级结构由α-螺旋向β-折叠和无规卷曲转变,蛋白的二、三级结构发生改变。此外,与β-LG相比,β-LG-RA共价偶联复合物的抗氧化性显著增强,与血清特异性Ig G的结合能力显著减弱。综上,RA共价偶联通过引入酚羟基增强了蛋白的抗氧化性,改变了β-LG的结构,降低了其与血清特异性IgG的结合能力。

关键词： β-乳球蛋白迷迭香酸共价偶联蛋白结构蛋白性质

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富铁大米对缺铁性贫血大鼠的改善作用

食品科技 2024年第5期49卷 147-153页

作者：付清王赵劼徐婷柯建杨宫智勇方敏武汉轻工大学食品科学与工程学院湖北省农产品加工与转化重点实验室湖北省高校人文社会科学重点研究基地食品安全研究中心湖北武汉430023

目的:缺铁性贫血(Iron deficiency anemia,IDA)是较严重的营养问题之一。一般大米每千克含铁量不超过2.0 mg。研究选取的某品牌大米,含铁量为5.33 mg/kg,高于普通大米铁含量2~3倍。将该富铁大米掺入饲料中,制备55%富铁大米饲料(富铁大... 详细信息

目的:缺铁性贫血(Iron deficiency anemia,IDA)是较严重的营养问题之一。一般大米每千克含铁量不超过2.0 mg。研究选取的某品牌大米,含铁量为5.33 mg/kg,高于普通大米铁含量2~3倍。将该富铁大米掺入饲料中,制备55%富铁大米饲料(富铁大米替代正常饲料中的淀粉)。评估该富铁大米对IDA大鼠的影响效果。方法:采用给予低铁饲料结合定期少量剪尾放血的方法建立IDA大鼠模型。选取正常饲料喂养的同龄健康大鼠作为空白对照组;将IDA大鼠随机分为正常饲料组、阳性对照组、低铁饲料组、富铁大米饲料组。实验结束时观察动物的生长体态情况,再分别测定各组动物的脏器指数、血红蛋白、血清铁、肝脏和脾脏的铁含量。结果:与正常饲料组相比,富铁大米饲料组大鼠体质量有所升高,心脏、脾脏指数均显著降低(P<0.05),血红蛋白水平显著升高(P<0.05)。此外,富铁大米饲料组的血清铁、肝、脾中铁含量较正常饲料组显著升高(P<0.05)。结论:该富铁大米对IDA大鼠的贫血有改善作用。

关键词：富铁大米缺铁性贫血血红蛋白血清铁

Subwavelength imaging and detection using adjustable and movable droplet microlenses

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Photonics Research 2020年第3期8卷 225-234页

作者： XIXI CHEN TIANLI WU zhiyong gong YUCHAO LI YAO ZHANG BAOJUN LI Institute of Nanophotonics Jinan UniversityGuangzhou 511443China

We developed adjustable and movable droplet microlenses consisting of a liquid with a high refractive *** microlenses were prepared via ultrasonic shaking in deionized water,and the diameter of the microlenses ranged from 1 to 50μ*** stretching the microlenses,the focal length can be adjusted from 13 to 25μ*** the assistance of an optical tweezer,controllable assembly and movement of microlens arrays were also *** results showed that an imaging system combined with droplet microlenses could image 80 nm beads under white light *** the droplet microlenses,fluorescence emission at 550 nm from Cd Se@Zn S quantum dots was efficiently excited and ***,Raman scattering signals from a silicon wafer were enhanced by^19 *** presented droplet microlenses may offer new opportunities for flexible liquid devices in subwavelength imaging and detection.

关键词： stretching illumination excited

协同育人模式下“食品毒理学”产学研一体化教学改革探讨

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农产品加工 2024年第11期 133-136页

作者：王桥刘言李玉芝宫智勇柳鑫武汉轻工大学食品科学与工程学院湖北武汉430023 武汉轻工大学湖北省农产品加工与转化重点实验室湖北武汉430023

协同育人模式下的“食品毒理学”产学研一体化教学改革,旨在通过深度整合高校、企业和科研机构资源,构建理论与实践紧密结合的教学体系。该改革通过重塑课程内容,强调真实案例分析与实践操作技能训练,提升学生解决复杂食品安全问题的能... 详细信息

协同育人模式下的“食品毒理学”产学研一体化教学改革,旨在通过深度整合高校、企业和科研机构资源,构建理论与实践紧密结合的教学体系。该改革通过重塑课程内容,强调真实案例分析与实践操作技能训练,提升学生解决复杂食品安全问题的能力,实现全程、全方位的人才培养。通过实证研究与数据分析,证明了此模式在提升教学质量、增强学生职业竞争力及服务产业需求等方面的显著效果,为我国“食品毒理学”教育的创新与发展提供可行路径。

关键词：食品毒理学协同育人产学研一体化教学改革

Angiotensin I converting enzyme(ACE)inhibitory activity and antihypertensive effects of rice peptides

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Food Science and Human Wellness 2022年第6期11卷 1539-1543页

作者： Jingjing Dong Shen Wang Xiaoyao Yin Min Fang zhiyong gong Yongning Wu Key Laboratory for Deep Processing of Major Grain and Oil of Ministry of Education College of Food Science and EngineeringWuhan Polytechnic UniversityWuhan 430023China HC Key Laboratory of Food Safety Risk Assessment Food Safety Research Unit(2019RU014)of Chinese Academy of Medical ScienceChina National Center for Food Safety Risk AssessmentBeijing 100021China

In this paper,the antihypertension effect of rice peptide(RP)was *** spontaneously hypertensive rats(SHR)and Wistar Kyoto(WKY)as the research objects,RP disposable gastric and long-term gastric irrigation experiments were carried out and systolic blood pressure(SBP)was *** the end of the long-term gastric irrigation experiment,the content of nitric oxide(NO),angiotensin-converting enzyme(ACE),angiotensin II(Ang II)and renin in the plasma and the activity of ACE were *** results showed that RP could reduce systolic pressure of SHR and had time-dose dependence while high-dose RP signifi cantly reduced systolic pressure by 24.6 and 17.2 mm Hg,respectively after a single and long-term gastric irrigation *** also could inhibit the activity of ACE and increase the release of *** results suggested that the decompression mechanism of RP is likely to be related to the regulation of the renin-angiotensin system(RAS)and NO.

关键词： Rice peptide Angiotensin-converting enzyme(ACE) Antihypertensive effect

Lipid droplets as endogenous intracellular microlenses

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Light(Science & Applications) 2021年第12期10卷 2507-2517页

作者： Xixi Chen Tianli Wu zhiyong gong Jinghui Guo Xiaoshuai Liu Yao Zhang Yuchao Li Pietro Ferraro Baojun Li Institute of Nanophotonics Jinan University511443 GuangzhouChina Department of Physiology School of MedicineJinan University510632 GuangzhouChina CNR-ISASI Institute of Applied Sciences and Intelligent Systems«E.Caianiello»Via Campi Flegrei 3480078 PozzuoliNaplesItaly

Using a single biological element as a photonic component with well-defined features has become a new intriguing paradigm in *** we show that endogenous lipid droplets in the mature adipose cells can behave as fully biocompatible microlenses to strengthen the ability of microscopic imaging as well as detecting intra-and extracellular *** the assistance of biolenses made of the lipid droplets,enhanced fluorescence imaging of cytoskeleton,lysosomes,and adenoviruses has been *** the same time,we demonstrated that the required excitation power can be reduced by up to 73%.The lipidic microlenses are finely manipulated by optical tweezers in order to address targets and perform their real-time imaging inside the *** efficient detecting of fluorescence signal of cancer cells in extracellular fluid was accomplished due to the focusing effect of incident light by the lipid *** lipid droplets acting as endogenous intracellular microlenses open the intriguing route for a multifunctional biocompatible optics tool for biosensing,endoscopic imaging,and single-cell diagnosis.

关键词： endogenous Lipid optics

Relationship between reactive oxygen species and sodium-selenite-induced DNA damage in HepG2 cells

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Frontiers of Medicine 2007年第3期1卷 327-332页

作者： ZOU Yunfeng NIU Piye gong zhiyong YANG Jin YUAN Jing WU Tangchun CHEN Xuemin Department of Occupational and Environmental Ministry of Education Key Laboratory of Environment and HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Selenium compounds,as an effective chemopreventive agent,can induce apoptosis in tumor *** oxygen species(ROS)are important mediators in apoptosis induced by various stimuli,which include chemopreventive *** this study,we investigated the relationship between ROS and the levels of DNA damage induced by selenite in HepG2 *** HepG2 cells were treated with selenite,there was a dose-dependent decrease in cell *** levels of ROS induced by selenite were measured by 2p,7p-dichlorofluorescein diacetate(DCFH-DA)fluorescence,which shows a dose-and time-dependent increase in HepG2 *** levels of DNA damage in HepG2 increased in all cells treated with an increasing dose of selenite at 0,2.5,5,10,and 20μmol/L.N-acetylcysteine(NAC),a known antioxidant,increased cell viability and decreased ROS ***,NAC effectively blocked DNA damage induced by *** results revealed that ROS might play an important role in selenite-induced DNA damage that can be reduced by NAC treatment.

关键词： cell survival DNA damage HepG2 cells reactive oxygen species sodium selenite

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提高酒糟利用率对出酒率及质量的影响研究

现代食品 2024年第2期30卷 56-58页

作者：袁霞龚志勇崇州市兴祥酒业有限责任公司四川崇州610000

酒糟是白酒生产过程中的副产物,其成分复杂,含有较高的蛋白质、糖分等物质,若不能及时处理,将会严重影响出酒率及出酒质量。因此,本文分析了酒糟利用率低这一问题,并通过实验得出了提高酒糟利用率的有效方法,旨在为企业提高经济效益提... 详细信息

酒糟是白酒生产过程中的副产物,其成分复杂,含有较高的蛋白质、糖分等物质,若不能及时处理,将会严重影响出酒率及出酒质量。因此,本文分析了酒糟利用率低这一问题,并通过实验得出了提高酒糟利用率的有效方法,旨在为企业提高经济效益提供理论基础。

关键词：酒糟出酒率出酒质量乙醇发酵