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Stem cell-laden hydrogel bioink for generation of high resolution and fidelity engineered tissues with complex geometries

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Bioactive Materials 2022年第9期7卷 185-193页

作者： oju Jeon yu Bin Lee Sang Jin Lee Nazilya Guliyeva Joanna Lee Eben Alsberg Department of Biomedical Engineering University of Illinois at ChicagoChicagoIL60607USA Department of Chemistry University of Illinois at ChicagoChicagoIL60607USA Department of Orthopaedics University of Illinois at ChicagoChicagoIL60607USA Department of Pharmacology University of Illinois at ChicagoChicagoIL60607USA Department of Mechanical and Industrial Engineering University of Illinois at ChicagoChicagoIL60607USA

Recently,3D bioprinting has been explored as a promising technology for biomedical applications with the potential to create complex structures with precise *** encapsulated hydrogels composed of materials such as gelatin,collagen,hyaluronic acid,alginate and polyethylene glycol have been widely used as bioinks for 3D ***,since most hydrogel-based bioinks may not allow rapid stabilization immediately after 3D bioprinting,achieving high resolution and fidelity to the intended architecture is a common challenge in 3D bioprinting of *** this study,we have utilized shear-thinning and self-healing ionically crosslinked oxidized and methacrylated alginates(OMAs)as a bioink,which can be rapidly gelled by its self-healing property after bioprinting and further stabilized via secondary *** was successfully demonstrated that stem cell-laden calcium-crosslinked OMA hydrogels can be bioprinted into complicated 3D tissue structures with both high resolution and *** photocrosslinking enables long-term culture of 3D bioprinted constructs for formation of functional tissue by differentiation of encapsulated human mesenchymal stem cells.

关键词： 3D bioprinting Hydrogel bioink Biomaterial High resolution and fidelity

Sensitive analysis of amino acids in injection liquor by reversed-phase HPLC

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Chinese Journal of Chemistry 1995年第2期13卷 163-168页

作者： SHANG, zh yu, YN GUO, W JIANG, H zhOU, LM CHINESE ACAD SCI DALIAN INST CHEM PHYSDALIAN 116012PEOPLES R CHINA

A convenient derivatization method of amino acids with 1-fluoro-2,4-dinitrobezene as reaction reagent and a separation system were described. The derivative amino acids were separated on a specific chemically bonded phase column with a simple linear gradient elution consisting of aqueous buffer and methanol. The eluate was detected by common ultraviolet absorption detector at 360 nm. The detection limits of amino acids were as low as 10 picomole. This method has been successfully applied to assay amino acid injection liquor used in hospital. It has good reproducibility and precision. The procedures avoid the requirements of particular derivative equipment and analyzer employed in conventional amino acid analysis.

关键词： AMINO ACIDS PRECOLUMN DERIVATIZATION REVERSED PHASE SEPARATION UV ABSORPTION DETERMINATION

Preparation and Application of Polymyxin B Affinity Membrane Cartridge

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Chinese Chemical Letters 1997年第8期8卷 745-746页

作者： Guo, W Shang, zh yu, YN zhou, LM zhou, K CHINESE ACAD SCI DALIAN INST CHEM PHYSDALIAN 116012PEOPLES R CHINA SHANGHAI MED UNIV ZHONGSHAN HOSPSHANGHAI 200032PEOPLES R CHINA

Affinity membrane was used for the endotoxin adsorption. The matrix macropore membrane was prepared from filterpaper by chemically crosslinking, and polymyxin B was immobilized as affinity ligand. The prepared membrane cartridge can be used for the removal of endotoxin from aqueous solutions. With a cartridge of 40 sheets affinity membrane containing, the endotoxin level of the processed solution can be reduced to a minimum of 4 pg/mL.

关键词： Preparation and Application of Polymyxin B Affinity Membrane Cartridge

Dip2a regulates stress susceptibility in the basolateral amygdala

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Neural Regeneration Research 2025年第6期20卷 1735-1748页

作者： Jing Li Zixuan He Weitai Chai Meng Tian Huali yu Xiaoxiao He Xiaojuan zhu Key Laboratory of Molecular Epigenetics Ministry of Education Institute of Genetics and CytologyNortheast Normal UniversityChangchunJilin ProvinceChina

Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.

关键词： 5-hydroxytryptamine acute restraint stress basolateral amygdala CaMKII neurons DIP2A metabolomics neurotransmitters principal component analysis stress susceptibility tryptophan

ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY

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Chinese Chemical Letters 1995年第10期6卷 919-922页

作者： Guo, W Shang, zh yu, YN zhou, LM Zun, ZY Ma, LR Chen, MY Chinese Acad Sci Dalian Inst Chem Phys Dalian 116012 Peoples R China Acad Mil Med Sci Inst Radiat Med Beijing 100850 Peoples R China

Endotoxin was removed by membrane affinity chromatography. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as stationary phase for affinity chromatography. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized as affinity ligand. A membrane cartridge containing 40 sheets of affinity membrane was also prepared, which can be used to remove pyrogen from aqueous solutions.

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Nuclear Factor-κB Signaling Mediates Antimony-induced Astrocyte Activation

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Biomedical and Environmental Sciences 2021年第1期34卷 29-39页

作者： zhANG Tao zhENG yu Dan JIAO Man zh Ye XU Shen Ya zhU Piao yu zhAO Xin yuan WU Qi yun Department of Occupational Medicine and Environmental Toxicology School of Public HealthNantong UniversityNantong 226019JiangsuChina Fudan University Taizhou Institute of Health Sciences Taizhou 225300JiangsuChina

Objective Antimony(Sb)has recently been identified as a novel nerve poison,although the cellular and molecular mechanisms underlying its neurotoxicity remain *** study aimed to assess the effects of the nuclear factor kappa B(NF-κB)signaling pathway on antimony-induced astrocyte *** Protein expression levels were detected by Western ***,cytoplasmic and nuclear fractions separation were used to assess the distribution of *** expression of protein in brain tissue sections was detected by *** levels of mRNAs were detected by Quantitative real-time polymerase chain reaction(qRT-PCR)and reverse transcriptionpolymerase chain reaction(RT-PCR).Results Antimony exposure triggered astrocyte proliferation and increased the expression of two critical protein markers of reactive astrogliosis,inducible nitric oxide synthase(iNOS)and glial fibrillary acidic protein(GFAP),indicating that antimony induced astrocyte activation in vivo and in *** exposure consistently upregulated the expression of inflammatory ***,it induced the NF-κB signaling,indicated by increased p65 phosphorylation and translocation to the ***-κB inhibition effectively attenuated antimony-induced astrocyte ***,antimony phosphorylated TGF-β-activated kinase 1(TAK1),while TAK1 inhibition alleviated antimonyinduced p65 phosphorylation and subsequent astrocyte *** Antimony activated astrocytes by activating the NF-κB signaling pathway.

关键词： Antimony Astrocyte activation Neurotoxicity NF-κB TAK1

Fast magnetic energy dissipation in relativistic plasma induced by high order laser modes

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High Power Laser Science and Engineering 2016年第2期4卷 85-89页

作者： Y.J.Gu Q.yu O.Klimo T.zh.Esirkepov S.V.Bulanov S.Weber G.Korn Institute of Physics of the ASCR Institute of Modern Physics Fudan University FNSPE Czech Technical University in Prague Kansai Photon Science Institute Japan Atomic Energy Agency

Fast magnetic field annihilation in a collisionless plasma is induced by using TEM(1,0) laser pulse. The magnetic quadrupole structure formation, expansion and annihilation stages are demonstrated with 2.5-dimensional particle-in-cell simulations. The magnetic field energy is converted to the electric field and accelerate the particles inside the annihilation plane. A bunch of high energy electrons moving backwards is detected in the current sheet. The strong displacement current is the dominant contribution which induces the longitudinal inductive electric field.

关键词： high order laser mode laser–plasma interaction magnetic annihilation

贵州罗甸玉“麻点”和“草花”的物质组成及成因研究

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光谱学与光谱分析 2024年第11期44卷 3236-3243页

作者：张可轩于海燕柏鹤张予烨桂林理工大学地球科学学院广西桂林541006 广西隐伏金属矿产勘查重点实验室广西桂林541006

选取带有“麻点”和“草花”花纹的贵州罗甸透闪石玉(罗甸玉)为研究对象,利用扫描电镜、电子探针和激光拉曼光谱仪测试技术,探讨罗甸玉中“麻点”和“草花”的物质组成和成因。测试结果表明:大的团块状“麻点”分布在部分方解石和透闪... 详细信息

选取带有“麻点”和“草花”花纹的贵州罗甸透闪石玉(罗甸玉)为研究对象,利用扫描电镜、电子探针和激光拉曼光谱仪测试技术,探讨罗甸玉中“麻点”和“草花”的物质组成和成因。测试结果表明:大的团块状“麻点”分布在部分方解石和透闪石的交界处或透闪石晶界处,小的片状“麻点”分布在方解石或透闪石的小的孔洞中。“麻点”的扫描电镜能谱和电子探针成分分析结果表明其由铁氧化物组成,拉曼光谱特征峰显示为赤铁矿。“草花”呈“沙漠玫瑰”的团球状或无定形状存在于两种结构透闪石交界处或柱状、片状透闪石的边缘。“草花”的扫描电镜能谱和电子探针成分分析结果表明其为锰氧化物,拉曼光谱特征峰显示为钙锰矿(640、356和287 cm^(-1))或水钠锰矿(636、582和506cm^(-1))。依据带有“麻点”和“草花”花纹的罗甸玉的成分和结构特征可以推测:罗甸玉中大的团块状“麻点”是后期富含Fe的热液与透闪石玉成矿热液混染成矿,而小的片状“麻点”则由富Fe热液充填形成。“草花”周围呈浸染状的叶片状或柱状透闪石是由富Mn热液与透闪石玉成矿热液混合结晶形成Mn含量比较高的透闪石,后期富Mn热液运移到柱状或片状透闪石的边缘或裂隙处析出形成钙锰矿或水钠锰矿。

关键词：罗甸玉麻点草花成分微形貌

On the State of the Soil Microflora in the Areas Separating from Launch Vehicle in Central Kazakhstan

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Journal of Life Sciences 2018年第5期12卷 224-230页

作者： zhubatov zh Stepanova Ye.yu Bekeshev E.A Toktar М Omaraly A Atygayev Anuar Bulatovich Republican state interprise Scientific Research Centre “Garysh-Ecology” Almaty A05A1K0 (050046)Kazakhstan North Kazakhstan State University named after Manash Kozybaev (NKSU)Petrapavl 150000Kazakhstan

The results of the comparative analysis of physical, chemical and biological characteristics of the soil cover at the sites of the first stage of the proton launch vehicle (LV) fall in the yu-2 zone (Central Kazakhstan) are presented. Conclusions are made about the increased sensitivity of the microflora of brown, mostly solonetzic soils to mechanical and chemical loads during the fall of the separating parts of space rockets. Lack of moisture, increased carbonate content and density of saline in the upper horizons complicate the restoration of the disturbed fertile soil layer even 10 years after the impact of rocket and space activities (SRA).

关键词： Rocket and space activity Central Kazakhstan solonetzic soils microflora stability chemical pollution.