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S100 protein expression during induced Schwann cell-like cell differentiation of rat bone marrow mesenchymal cells in vitro

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Neural Regeneration Research 2010年第3期5卷 178-184页

作者： Wenting Li zenglu xu Fei Ding Xiaosong Gu Institute of Basic Medical Science Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100005 China Jiangsu Key Laboratory of Neuroregeneration Nantong University Nantong 226001 Jiangsu Province China

BACKGROUND： S100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells （MSCs） that can secrete S100 may provide a beneficial microenvironment for neural regeneration. OBJECTIVE： To explore the changes in S100 expression during rat MSCs differentiation into Schwann ceils in vitro. DESIGN, TIME AND SETTING： This cytology experiment was performed at the Jiangsu Key Laboratory of Neuroregeneration, Nantong University in China, from January 2006 to May 2007. MATERIALS： The rabbit anti-S100 polyclonal antibody was purchased from Dako, Denmark; the mouse anti-rat S100 monoclonal antibody was purchased from Sigma, USA. METHODS： MSCs were cultured from adult Sprague-Dawley rat femur and tibia. Cell proliferation was determined by the MTT method and CD markers, and cell cycle was measured by flow cytometry. MSCs were induced to differentiate into SC cells. SC cells were stained for S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. S100 protein and mRNA levels were evaluated by flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. MAIN OUTCOME MEASURES： S100 protein and mRNA expression. RESULTS： MSCs exhibited high amplification potential over eight passages. Prior to induction, the majority of MSCs were at the G0/G1 phase of the cell cycle. After induction, MSCs displayed morphology changes similar to Schwann cells. Moreover, induction increased S100 mRNA levels. Immunofluorescence showed that MSCs expressed S100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor at 7 days of induction. Induction also increased S100 protein levels compared with untreated MSCs. CONCLUSION： MSCs are capable of differentiating into Schwann cells-like cells under conditional induction in vitro, with increasing S100 mRNA and protein expression.

关键词： bone marrow mesenchymal stem cells induction Schwann cell-like cells S100 protein in vitro stem cells neural regeneration

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烧伤湿性医疗技术对表皮再生干细胞作用的研究

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中国烧伤创疡杂志 2000年第3期12卷 41-43页

作者：徐荣祥许增禄中国烧伤创疡科技中心 100053

目的 :探索烧伤湿性医疗技术于浅Ⅲ度烧伤时脂肪层烧伤创面自然修复的机制。方法 :应用细胞免疫化学方法生物素抗生物素蛋白 (biotinavidin)DCS体系间接免疫荧光技术。以小鼠抗人角蛋白 1 9的单克隆抗体检测表皮再生干细胞。结果 :烧伤... 详细信息

目的 :探索烧伤湿性医疗技术于浅Ⅲ度烧伤时脂肪层烧伤创面自然修复的机制。方法 :应用细胞免疫化学方法生物素抗生物素蛋白 (biotinavidin)DCS体系间接免疫荧光技术。以小鼠抗人角蛋白 1 9的单克隆抗体检测表皮再生干细胞。结果 :烧伤后2 4h可见表皮再生干细胞出现。烧伤后 4天表皮再生干细胞数量增多。烧伤后 7天和 1 4天表皮再生干细胞数量最多。烧伤后 2 1天和 2 8天表皮再生干细胞减少。结论

关键词：烧伤湿性医疗技术表皮干细胞细胞免疫化学

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实验性隐睾诱导小鼠生精细胞凋亡的研究

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解剖学报 1999年第1期30卷 82-85,I018页

作者：徐健许增禄姜英涛钱晓菁黄玉苓中国医学科学院中国协和医科大学基础医学研究所

目的研究手术隐睾所致成年小鼠生殖细胞凋亡及相关调节因子ｂｃｌ－２、ｂａｘ蛋白在曲细精管中的定位、变化。方法以末端脱氧核糖核酸转移酶介导的ｄＵＴＰ缺口末端标记法（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎ... 详细信息

目的研究手术隐睾所致成年小鼠生殖细胞凋亡及相关调节因子ｂｃｌ－２、ｂａｘ蛋白在曲细精管中的定位、变化。方法以末端脱氧核糖核酸转移酶介导的ｄＵＴＰ缺口末端标记法（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎｓ－ｆｅｒａｓｅ－ｍｅｄｉａｔｅｄｄＵＴＰｎｉｃｋ－ｅｎｄｌａｂｅｌｉｎｇｍｅｔｈｏｄ，ＴＵＮＥＬ）检测凋亡的生殖细胞，生物素－抗生物素蛋白ＤＣＳ（ｂｉ－ｏｔｉｎ－ａｖｉｄｉｎＤｃｅｌｓｏｒｔｅｒ）体系间接免疫荧光法检测Ｂｃｌ－２、Ｂａｘ蛋白在曲细精管中的定位、分布。结果隐睾术后３ｄ，手术侧睾丸重量及细胞凋亡数量与对侧无明显区别。而６～１５ｄ，睾丸重量明显减轻，凋亡细胞明显增多，以精母细胞、精子细胞为主。在正常小鼠曲细精管中，ｂｃｌ－２大量表达，呈散在的颗粒状存在于各级生精细胞中，以精子细胞胞质为主。Ｂａｘ蛋白表达量较少，一部分也以散在的颗粒存在于各级生精细胞质中，另一部分则较致密地分布于曲细精管基底部。隐睾术后３ｄ，Ｂｃｌ－２、Ｂａｘ的表达与对侧无明显区别；６～１５ｄ，Ｂｃｌ－２明显下降，Ｂａｘ明显增加。结论隐睾明显诱导生殖细胞凋亡，Ｂｃｌ－２、Ｂａｘ蛋白对生殖细胞的凋亡有重要调节作用，提示温?

关键词：生精细胞细胞凋亡隐睾小鼠

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小鼠附睾Ⅳ型胶原和层粘连蛋白的分布

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解剖学报 1999年第3期30卷 273-275,I018页

作者：崔丽许增禄刘霄徐健钱晓菁叶惟三黄玉苓中国医学科学院中国协和医科大学基础医学研究所

目的研究附睾发育过程中Ⅳ型胶原和层粘连蛋白（ｌｉａｍｉｎｉｎ，ＬＮ）的定位和分布。方法应用生物素－抗生物素ＤＣＳ体系间接免疫荧光染色技术。结果１５ｄ幼鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度较弱；３个月成年鼠附睾基膜内Ⅳ型... 详细信息

目的研究附睾发育过程中Ⅳ型胶原和层粘连蛋白（ｌｉａｍｉｎｉｎ，ＬＮ）的定位和分布。方法应用生物素－抗生物素ＤＣＳ体系间接免疫荧光染色技术。结果１５ｄ幼鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度较弱；３个月成年鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度最强；１８个月老年鼠附睾基膜内Ⅳ型胶原和ＬＮ荧光强度比３个月弱。３月龄成年小鼠附睾各部分的荧光强度从头部至尾部呈逐渐增强的趋势。结论小鼠附睾内的细胞外基质成分可能与附睾各部分功能状态有密切关系。

关键词：附睾 Ⅳ型胶原层粘连蛋白小鼠

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石棉诱导细胞凋亡改变的研究

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中华劳动卫生职业病杂志 1999年第3期17卷 145-147页

作者：刘秉慈黄筚辉许增禄徐健王海华尤宝荣缪庆徐茗中国预防医学科学院劳动卫生与职业病研究所中国医学科学院中国协和医科大学基础医学研究所

目的探讨石棉诱导的细胞凋亡及其相关基因ｂｃｌ２及ｂａｘ表达的改变。方法大鼠石棉染尘。收集石蜡包埋的石棉作业工人肺癌组织块。ＴＵＮＥＬ法（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎｓｆｅｒａｓｅｍｅｄ... 详细信息

目的探讨石棉诱导的细胞凋亡及其相关基因ｂｃｌ２及ｂａｘ表达的改变。方法大鼠石棉染尘。收集石蜡包埋的石棉作业工人肺癌组织块。ＴＵＮＥＬ法（ｔｅｒｍｉｎａｌｄｅｏｘｙｎｕｃｌｅｏｔｉｄｙｌｔｒａｎｓｆｅｒａｓｅｍｅｄｉａｔｅｄｄＵＴＰｎｉｃｋｅｎｄｌａｂｅｌｉｎｇｍｅｔｈｏｄ，末端脱氧核糖核酸转移酶介导的ｄＵＴＰ缺口末端标记法）观察凋亡细胞分布及数量。生物素抗生物素蛋白ＤＣＳ（ａｖｉｄｉｎＤｃｅｌｓｏｒｔｅｒ）间接免疫荧光法观察ｂｃｌ２及ｂａｘ表达改变，琼脂糖凝胶电泳进行ＤＮＡ梯状电泳分析。结果大鼠染尘后４０天，细胞凋亡增强、ｂｃｌ２表达减少、ｂａｘ蛋白含量升高，可见ＤＮＡ特征性梯度电泳条带；大鼠染尘后７０天，细胞凋亡明显减弱。在石棉作业工人肺癌组织中未见明显细胞凋亡。结论石棉可诱导肺组织细胞凋亡发生改变，这种改变有时段性。凋亡相关基因表达也有相应变化。

关键词：石棉细胞凋亡 bcl-2 bax 肺癌职业

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Aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders:progress of experimental models based on disease pathogenesis

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Neural Regeneration Research 2025年第2期20卷 354-365页

作者： Li xu Huiming xu Changyong Tang Department of Neurology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor *** date,no effective treatment exists as the exact causative mechanism remains ***,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic *** most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets *** experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum *** this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model *** addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.

关键词： aquaporin-4 experimental model neuromyelitis optica spectrum disorder pathogenesis

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Reducing the measurement errors in nonadiabatic holonomic quantum computers

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 64-70页

作者： Guo-Fu xu Department of Physics Shandong University

Nonadiabatic holonomic quantum computers serve as the physical platform for nonadiabatic holonomic quantum *** quantum computation has entered the noisy intermediate-scale era, building accurate intermediate-scale nonadiabatic holonomic quantum computers is clearly necessary. Given that measurements are the sole means of extracting information, they play an indispensable role in nonadiabatic holonomic quantum computers. Accordingly, developing methods to reduce measurement errors in nonadiabatic holonomic quantum computers is of great importance. However, while much attention has been given to the research on nonadiabatic holonomic gates, the research on reducing measurement errors in nonadiabatic holonomic quantum computers is severely lacking. In this study, we propose a measurement error reduction method tailored for intermediate-scale nonadiabatic holonomic quantum computers. The reason we say this is because our method can not only reduce the measurement errors in the computer but also be useful in mitigating errors originating from nonadiabatic holonomic gates. Given these features,our method significantly advances the construction of accurate intermediate-scale nonadiabatic holonomic quantum computers.

关键词： quantum holonomy quantum computation measurement errors

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Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

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Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi xu Junqiu Jia Rui Mao Xiang Cao Yun xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a comprehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality control mitophagy spinal cord injury subarachnoid hemorrhage traumatic brain injury

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Enantio-divergent C–H borylation with two different ligands from a single chiral source

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Science China Chemistry 2025年第1期 226-232页

作者： Rongrong Du Senmiao xu State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of Chemical Physics Chinese Academy of Sciences College of Material Chemistry and Chemical Engineering Key Laboratory of Organosilicon Chemistry and Material TechnologyMinistry of Education Hangzhou Normal University University of Chinese Academy of Sciences

Reported here is an iridium-catalyzed enantio-divergent C（sp3）–H borylation of α-gem-dimethyl groups of trifluoromethyl ketone-derived hydrazones to construct a series of acyclic all-carbon quaternary stereocenters with a high level of chiral induction. Two different chiral bidentate boryl ligands from a common chiral source not only can switch the enantioselectivity but also are complementary in terms of the substrate scope.

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A surface engineering strategy for the stabilization of zinc metal anodes with montmorillonite layers toward long-life rechargeable aqueous zinc ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 94-105页

作者： Wenbo Wang Ruifeng xu xu Zhang Peiyu Wang Bao Yang Bingjun Yang Juan Yang Kailimai Su Pengjun Ma Yanan Deng Xianfeng Fan Wanjun Chen Key Laboratory for Electronic Materials College of Electrical Engineering Northwest Minzu University Resource Chemistry and Energy Materials Research Center State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Institute for Materials and Processes School of Engineering University of Edinburgh

Rechargeable aqueous zinc-ion batteries （AZIBs） exhibit appreciable potential in the domain of electrochemical energy ***,there are serious challenges for AZIBs,for instance zinc dendrite growth,hydrogen evolution reaction （HER）,and corrosion side ***,we propose a surface engineering modification strategy for coating the montmorillonite （MMT） layer onto the surface of the Zn anode to tackle these issues,thereby achieving high cycling stability for rechargeable *** results reveal that the MMT layer on the surface of the Zn anode is able to provide ordered zincophilic channels for zinc ions migration,facilitating the reaction kinetics of zinc *** functional theory（DFT） calculations and water contact angle （CA） tests prove that MMT@Zn anode exhibits superior adsorption capacity for Zn2+and better hydrophobicity than the bare Zn anode,thereby achieving excellent cycling ***,the MMT@Zn||MMT@Zn symmetric cell holds the stable cycling over 5600 h at 0.5 mA cm-2and 0.125 m A h cm-2,even exceeding 1800 h long cycling under harsh conditions of 5 m A cm-2and 1.25 m A h *** MMT@Zn||V2O5full cell reaches over 3000 cycles at 2 A g-1with excellent rate ***,this surface engineering modification strategy for enhancing the electrochemical performance of AZIBs represents a promising application.

关键词： Surface engineering strategy Montmorillonite layer Protection mechanism of Zn anode Cycling stability Aqueous zinc ion batteries

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