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PIG3 downregulation enhances the radiosensitivity of NSCLC cells by promoting G_(2)/M cell cycle arrest and apoptosis

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Radiation Medicine and Protection 2023年第1期4卷 19-25页

作者： Hao Zhou Linfeng Tang zeng-fu shang Ping-Kun Zhou Ming Li State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionMedical College of Soochow UniversitySuzhou 215123China Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow UniversitySuzhou 215123China Department of Radiation Toxicology and Oncology Beijing Key Laboratory for RadiobiologyBeijing Institute of Radiation MedicineBeijing 100850China

Objective:To investigate the mechanism of p53-induced gene 3(PIG3)-regulation of radioresistance in human non-small cell lung cancer(NSCLC)cells,in order to explore new biomarkers and therapeutic targets to combat radioresistance and improve the 5-year survival rate.Methods:The PIG3 gene was knocked down in A549 cells using siRNA,and was overexpressed in H1299 cells using a PIG3 expression plasmid.After confirming PIG3 knockdown and overexpression through the Western blot analysis,the radiosensitivity,DNA damage,cell cycle distribution,and apoptosis in these cells were analyzed using colony formation assay,immunofluorescence staining forγH2AX,and flow cytometry,respectively.Results:PIG3 silencing markedly increased the radiosensitivity of NSCLC cells,with radiosensitization ratios of 1.12 and 1.25.Compared with the corresponding negative control,PIG3 knockdown significantly enhanced G2/M phase arrest(siNC:26.12±2.50,siPIG3#1:34.98±4.19,siPIG3#2:37.79±3.53,P<0.05),promoted radiation-induced apoptosis(siNC:14.61±1.85,siPIG3#1:17.26±1.14,siPIG3#2:20.70±2.04,P<0.05),and reduced the number ofγ-H2AX foci 0.5,1,and 2 h after radiation(P<0.05).Conversely,PIG3 overexpression markedly decreased the radiosensitivity of NSCLC cells,as evidenced by the reduction of G2/M phase arrest(NC:33.18±2.11 vs.PIG3:24.21±3.09,P<0.05)and apoptosis(NC:15.49±0.56 vs.PIG3:12.79±0.29,P<0.05),and increased DNA damage(P<0.05).Conclusions:PIG3 downregulation increases the radiosensitivity of NSCLC cells,and PIG3-upregulation leads to the progression in radioresistance.Therefore,PIG3 is a potential target for radiotherapy for NSCLC.

关键词： Radioresistance PIG3 Non-small cell lung cancer Cell cycle Apoptosis

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Freestanding MoSe_(2)nanoflowers for superior Li/Na storage properties

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Tungsten 2024年第1期6卷 238-247页

作者： Qiao Cu Chao-Qun shang Guo-fu Zhou Xin Wang Guangdong Provincial Key Laboratory of Optical Information Materials South China Normal UniversityGuangzhou 510006China School of Materials Science and Engineering and Hubei Key Laboratory of Plasma Chemistry and Advanced Materials Wuhan Institute of TechnologyWuhan 430205China International Academy of Optoelectronics at Zhaoqing South China Normal UniversityZhaoqing 526060China

MoSe_(2),with high theoretical specific capacity,has attracted a lot of attention.There remains an open challenge to effectively suppress the irreversible selenium dissolution and rapid capacity decrease induced by severe volume change during cycling.Herein,we synthesize MoSe_(2)nanoflowers dispersed on one-dimensional(1D)N-doped carbon nanofibers(MoSe_(2)@NCNFs)for use as a freestanding electrode.In this unique structure,the 1D N-doped carbon nanofibers are found to not only enhance the conductivity but also ensure the structural integrity during the Li^(+)/Na^(+)insertion/destraction processes.As expected,at 2 A·g^(-1),the specific capacity of the MoSe_(2)@NCNFs is maintained at 180 mAh·g^(-1)after 500 cycles when used in lithium storage applications.furthermore,in the case of sodium storage,at 1 A·g^(-1),the MoSe_(2)@NCNFs shows a capacity of 122mAh·g^(-1)after 500 cycles.These findings suggest that the MoSe_(2)@NCNF electrodes may be a promising candidate for use in reversible Li/Na storage applications.

关键词： Li/Na storage Freestanding electrode MoSe_(2) Electrochemical reaction kinetics Structural integrity

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Discharge evolution law of debris flow based on a sharp bend physical modeling test

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Journal of Mountain Science 2024年第6期21卷 1904-1915页

作者： LU Ming SUN Hao LIU Jinfeng Abrar HUSSAIN shang Yuqi fu Hang Key Laboratory of Mountain Hazards and Earth Surface Process Chinese Academy of SciencesChengdu 610041China Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China

For the basins with debris flow development,its channel terrain exhibits a tortuous shape,which significantly restricts the movement of debris flows and leads to severe erosion effects on the concave bank.Therefore,this study aims to analyze the shear force of debris flows within the bend channel.We established the relationship between the shear force and bend curvature through laboratory experiments.Under the long-term erosion by debris flows,the curvature radius of bends gradually increases,however,when this increasing trend reaches an equilibrium state with the intensity of debris flow discharge,there will be no significant change in curvature radius.In general,the activity pattern and discharges of debris flows would remain relatively stable.Hence,we can infer the magnitude of debris flow discharges from the terrain parameters of the bend channel.

关键词： Debris flow discharge Erosion effect Bend channel Curvature radius

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CFD simulation of pumping ventilation in a three-story isolated building with internal partitioning:Effects of partition widths,heights and locations

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Building Simulation 2024年第2期17卷 267-284页

作者： Huai-Yu Zhong Jie Sun Chao Lin Song-Heng Wu Jin shang Hideki Kikumoto fu-Ping Qian Carlos Jimenez-Bescos fu-Yun Zhao School of Energy and Environment Anhui University of TechnologyMa-An-ShanAnhui Province243002China State Key Laboratory of Green Building in Western China Xi’an University of Architecture&TechnologyXi’anShaanxi ProvinceChina School of Power and Mechanical Engineering Wuhan UniversityWuhanHubei Province430072China Institute of Industrial Science The University of Tokyo4-6-1 KomabaMeguro-kuTokyo153-8505Japan School of Built and Natural Environment University of DerbyDerbyUK

Pumping ventilation(PV),a special single-sided ventilation(SSV),has been certified as an effective strategy to improve the air exchange rate of SSV.However,most studies targeted on the single space,and few studies have been focused on the effect of internal partitioning on PV.This paper aims to evaluate the ventilation performance of PV influenced by different configurations of internal partitioning.Computational fluid dynamics(CFD)simulation was used to predict the flow fields and ventilation rates.The width(w/H),height(h/H)and location(d/H)are the three main internal partition parameters considered in this study.The simulation results showed that the total,mean and fluctuating ventilation rates all decrease with wider internal partitions.The normalized total ventilation rate decreases by 7.6%when w/H is increased from 50%to 75%.However,the reduction rate is only 0.23%between w/H=0 and 25%,and only 0.61%between w/H=25%and 50%.The ventilation rate is hardly reduced by increasing the partition width when w/H50%.Increasing the partition height will reduce the mean ventilation rate but promote the fluctuating and total ventilation rate in some cases.An increase of total ventilation rate by 1.4%is observed from h/H=50%to 75%.The ventilation rate is larger when the internal partition is attached to the leeward or windward wall.The total,mean and fluctuating ventilation rates for d/H=50%are relatively higher than d/H=0 by 1.5%,3.1%and 0.8%,respectively.Hence the internal partition should be mounted attached to the windward wall so as to obtain the greatest pumping ventilation rate.The periodicity of pumping flow oscillation and pumping frequency are independent of the partition configurations.The peak power of pumping flow is the lowest for the widest internal partition and is negatively affected by the partition height,but it generally has a positive correlation with the distance between the partition and leeward wall.Present research will help to understand pump

关键词： pumping ventilation single-sided ventilation internal partitioning periodic oscillation ventilation rate

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Application of a concrete thermal pile in cooling the warming permafrost under climate change

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Advances in Climate Change Research 2024年第1期15卷 170-183页

作者： Yun-Hu shang fu-Jun NIU Guo-Yu LI Jian-Hong FANG Ze-Yong GAO State Key Laboratory of Frozen Soil Engineering Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China Da Xing'anling Observation and Research Station of Frozen-Ground Engineering and Environment Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesJagdaqi 165000China Qinghai Research Institute of Transportation Xining 810008China

Permafrost degradation caused by climate warming is posing a serious threat to the stability of cast-in-place pile foundations in warm permafrost regions.Ambient cold energy can be effectively utilized by two-phase closed thermosyphons(TPCTs)to cool the permafrost.Therefore,we installed TPCTs in a cast-in-place pile foundation to create a unique structure called a thermal pile,which effectively utilizes the TPCTs to regulate ground temperature.And we conducted a case study and numerical simulation to exhibit the cooling performance,and optimize the structure of the thermal pile.The purpose of this study is to promote the application of thermal piles in unstable permafrost regions.Based on the findings,the thermal pile operated for approximately 53%of the entire year and effectively reduced the deep ground temperature at a rate of at least-0.1℃per year.Additionally,it successfully raised the permafrost table that is 0.35 m shallower than the natural ground level.These characteristics prove highly beneficial in mitigating the adverse effects of permafrost degradation and enhancing infrastructure safety.Expanding the length of the condenser section and the diameter of the TPCT in a suitable manner can effectively enhance the cooling capability of the thermal pile and ensure the long-term mechanical stability of the pile foundation even under climate warming.

关键词： Heat transfer Cold energy Warm permafrost Two-phase closed thermosyphons Cast-in-place pile foundation

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Predictive performance of the variation rate of the driving pressure on the outcome of invasive mechanical ventilation in patients with acute respiratory distress syndrome

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Chinese Journal of Traumatology 2024年第2期27卷 107-113页

作者： Hui-Dan Jing Jun-Ying Tian Wei Li Bing-Ling He Hong-Chao Li fu-Xia Jian Cui shang Feng Shen Department of Intensive Care Unit The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China Department of Intensive Care Unit Army Medical Center of PLAChongqing400042China College of Foreign Languges Chongqing Medical UniversityChongqing400016China

Purpose:To assess the value of the driving pressure variation rate(ΔP%)in predicting the outcome of weaning from invasive mechanical ventilation in patients with acute respiratory distress syndrome.Methods:In this case-control study,a total of 35 patients with moderate-severe acute respiratory distress syndrome were admitted to the intensive care unit between January 2022 and December 2022 and received invasive mechanical ventilation for at least 48 h were enrolled.Patients were divided into successful weaning group and failed weaning group depending on whether they could be removed from ventilator support within 14 days.Outcome measures including driving pressure,PaO2:FiO2,and positive end-expiratory pressure,etc.were assessed every 24 h from day 0 to day 14 until successful weaning was achieved.The measurement data of non-normal distribution were presented as median(Q1,Q3),and the differences between groups were compared by Wilcoxon rank sum test.And categorical data use the Chi-square test or Fisher's exact test to compare.The predictive value ofΔP%in predicting the outcome of weaning from the ventilator was analyzed using receiver operating characteristic curves.Results:Of the total 35 patients included in the study,17 were successful vs.18 failed in weaning from a ventilator after 14 days of mechanical ventilation.The cut-off values of the medianΔP%measured by Operator 1 vs.Operator 2 in the first 4 days were≥4.17%and 4.55%,respectively(pful weaning group and the failure weaning group(8(6,13)vs.12(7.5,17.3),p=0.043).The incidence of ventilator-associated pneumonia in the successful weaning group was significantly lower than in the failed weaning group(0.2‰vs.2.3‰,p=0.001).There was a significant difference noted between these 2 groups in the 28-day mortality(11.8%vs.66.7%,

关键词： Driving pressure Driving pressure variation rate Acute respiratory distress syndrome Mechanical ventilation

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Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia

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Animal Models and Experimental Medicine 2024年第1期7卷 24-35页

作者： Xiaojun Deng Xiaoyi Hu shang Wang Hui Zhao Yaqin Wei Jiaqi fu Wenhui Wu Jinming Liu Caicai Zhang Lili Wang Ping Yuan Department of Critical Care Medicine Shanghai Sixth People's HospitalShanghai Jiao Tong UniversityShanghaiChina Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Institute of Bismuth Science University of Shanghai for Science and TechnologyShanghaiChina Department of Physiology Hainan Medical University HaikouHainanChina Department of Clinical Medical Laboratory Center Hangzhou Red Cross HospitalZhejiangChina

Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.

关键词： brain microvascular endothelial cells exosomes Hes1 miR-9 neural stem cells

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Polysaccharides of Aspergillus cristatus attenuate obesity by regulating gut microbiota and gut microbiota-related metabolites

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Food Science and Human Wellness 2024年第3期13卷 1513-1530页

作者： Mingzhi Zhu Bohao shang Fang Zhou Yong Yuan Feiyan Yin Jin Cao Jianan Huang Kunbo Wang Xin zeng Maiquan Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China College of Life Sciences Huaibei Normal UniversityHuaibei 235000China Hunan Tea Group Co. Ltd.Changsha 410128China School of Food Science and Technology Hunan Agricultural UniversityChangsha 410128China

Golden-flower fungus,the only dominant microorganism determining the fu-brick tea quality through fermentation and the important microbe in Liupao tea,is considered a potential probiotic fungus based on its anti-obesity effect.However,the classification of golden-flower fungi is still controversial;the anti-obesity effect of golden-flower fungus polysaccharides remains unknown.In this study,we identify a golden-flower strain as Aspergillus cristatus based on morphological characteristics and multigene phylogeny analysis,which resolves the controversy of classification.Moreover,we find A.cristatus polysaccharides(ACPS)attenuate obesity in rats.ACPS modulate gut bacterial composition.in which Akkermansia,Akkermansia muciniphila,Bacteroides,Romboutsia,Blautia,and Desulfovibrio are considered the core microbes regulated by ACPS.ACPS increase fecal total short-chain fatty acid content and serum,hepatic,and fecal total bile acid content.furthermore,ACPS-induced gut microbiota alteration plays a causal role in the protection from obesity,according to a fecal transplantation experiment.Thus,ACPS ameliorate obesity by regulating gut microbiota and gut microbiota-related metabolites.

关键词： Golden-flower fungus Aspergillus cristatus Polysaccharides Obesity Gut microbiota

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分子视角下的电子自旋——自旋化学开拓合成化学科学前沿

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中国科学：化学 2024年第3期54卷 277-286页

作者：蒋尚达高松付雪峰华南理工大学自旋科技研究院广州511442 中山大学化学学院广州510275 北京大学化学与分子工程学院北京100871 国家自然科学基金委员会化学科学学部北京100085

本文从化学过程和分子材料的自旋效应、分子自旋材料新物态、分子自旋诊疗药物以及自旋化学理论计算等方面介绍了自旋化学的概念和研究领域,并对自旋化学面临的挑战和未来的重点研究方向进行了讨论.

关键词：交叉学科自旋化学分子磁性自旋效应自旋材料

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面孔吸引力对经济决策的影响机制

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科学通报 2024年第11期69卷 1447-1460页

作者：尚俊辰张懿倬陈文锋傅小兰东南大学人文学院医学人文学系南京211189 中国人民大学心理学系北京100872 中国科学院心理研究所脑与认知科学国家重点实验室北京100101 中国科学院大学心理学系北京100049

面孔吸引力影响人类的经济决策,人们对高吸引力和低吸引力博弈对象的决策不同.大量研究报告美貌溢价效应,另一些研究则发现美貌惩罚效应.前人提出经济学、社会心理学和进化心理学3个取向来解释面孔吸引力影响决策的原因.经济学取向和社... 详细信息

面孔吸引力影响人类的经济决策,人们对高吸引力和低吸引力博弈对象的决策不同.大量研究报告美貌溢价效应,另一些研究则发现美貌惩罚效应.前人提出经济学、社会心理学和进化心理学3个取向来解释面孔吸引力影响决策的原因.经济学取向和社会心理学取向都只提供了描述性解释,忽略了性别的作用.进化心理学取向则提出更深层次的解释,认为人类对高吸引力面孔的偏好主要受到择偶动机的驱使,且人们更偏爱高吸引力的异性.虽然这3个理论体系的解释层级不同,但并不互斥,它们在预测不存在性别差异的美貌溢价现象时有共同的解释力.电生理研究进一步从决策过程中面孔吸引力的认知加工和对公平以及风险的感知的影响方面揭示面孔吸引力影响经济决策的神经生物学机制,发现高吸引力面孔在决策过程中能诱发较大的晚期正成分,并激活伏隔核、眶额叶皮层、腹侧纹状体等与奖赏加工有关的脑区.未来研究应探究真实经济决策情境中,面孔与嗓音、气味等多通道吸引力的交互作用,建立不同面孔吸引力水平的动态连续变化模型,并将电生理研究与现有理论体系整合,全面阐释吸引力影响经济决策的神经机制,并充分挖掘面孔吸引力影响决策的中介变量和调节变量,为控制招聘等活动中的外貌偏见提供理论依据.

关键词：面孔吸引力经济决策美貌溢价美貌惩罚神经机制

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