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Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

Molecular Plant 2024年第1期17卷 158-177页

作者： Peng Li Meng-Xiao Yan Pan Liu Dan-Jie Yang ze-kun he Yun Gao Yan Jiang Yu Kong Xin Zhong Sheng Wu Jun Yang Hong-Xia Wang Yan-Bo Huang Le Wang Xiao-Ya Chen Yong-Hong Hu Qing Zhao Ping Xu shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChineseAcademy of SciencesShanghaiChina College of Life Sciences Shanghai Normal UniversityShanghai 200234China State Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina University of the Chinese Academy of Sciences Beijing 100049China The Research Center of Chiral Drugs Innovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai 201203China College of Chemistry and Chemical Engineering Shanghai University of Engineering ScienceShanghai 201620China

The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich ***,the principal active compound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating ***,the biosynthetic pathway of leonurine remains largely ***,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine *** integrating genomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltransferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reactions in this *** analyses revealed that the UGT–SCPL gene cluster evolved by gene duplication in the ancestor of Leonurus and neofunctionalization of SCPL in ***,which contributed to the accumulation of leonurine specifically in ***,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.

关键词： Lamiaceae Leonurus japonicus Leonurus sibiricus genome sequence alkaloids leonurine gene cluster biosynthetic pathway evolution

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Understand the Temperature Sensing Behavior of Solid-state Polymerized PEDOT Hybrid Based on X-ray Scattering Studies

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Chinese Journal of Polymer Science 2024年第1期42卷 105-112,I0010页

作者： Zhen-Hang he Guang-Feng Liu ze-kun Zhou Zhen Liu Yi-Shu zeng Peng Zhang School of Materials Science and Engineering Key Laboratory for Polymeric Composite and Functional Materials of Ministry of EducationSun Yat-sen UniversityGuangzhou 510275China Shanghai Advanced Research Institute(Zhangjiang Laboratory) Chinese Academy of SciencesShanghai 201204China

Poly(3,4-ethylenedioxythiophene)(PEDOT) is one of the most successful conductive polymers that recently has been used in wearable sensors for human health monitoring. In this work, we prepared a series of PEDOT hybrids consisting of PEDOT, sodium poly(styrene sulfonate)(PSSNa) and polyethylene oxide(PEO), and their preparation could be scaled-up via an adapted solid-state polymerization process. The resistance of the as-prepared PEDOT:PSS/PEO hybrid shows clear temperature response, i.e., it decreases almost linearly with the temperature increase. To understand this phenomenon, the in situ synchrotron radiation wide-and small-angle X-ray scattering(WAXS/SAXS) characterizations were undertaken to study the temperature-dependent microstructure change of the PEDOT:PSS/PEO hybrid. It demonstrated that PEDOT formed conductive paths in the hybrids, which were not destroyed by the PEO crystallization. As temperature increased, the PEO crystals' melting and the accompanying reorganization of PEDOT chains endowed the hybrid sample temperature responsiveness. Based on these fundamental knowledges, the hybrid materials were used to fabricate flexible wearable sensor that showing temperature sensing performance with an accuracy of 1 ℃. These findings shed lights on the scalable manufacturing of wearable sensors for body temperature monitoring.

关键词： Solid-state polymerization PEDOT hybrid Structure-property correlation X-ray scattering

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Benchmarking visual SLAM methods in mirror environments

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Computational Visual Media 2024年第2期10卷 215-241页

作者： Peter herbert Jing Wu ze Ji Yu-kun Lai School of Computer Science and Informatics Cardiff UniversityAbacws BuildingSenghennydd RdCardiff CF244AGUK School of Engineering Cardiff UniversityQueen’s BuildingsThe ParadeCardiff CF243AAUK

Visual simultaneous localisation and mapping(vSLAM)finds applications for indoor and outdoor navigation that routinely subjects it to visual complexities,particularly mirror *** effect of mirror presence(time visible and its average size in the frame)was hypothesised to impact localisation and mapping performance,with systems using direct techniques expected to perform ***,a dataset,MirrEnv,of image sequences recorded in mirror environments,was collected,and used to evaluate the performance of existing representative *** ORB-SLAM3 and BundleFusion appear to show moderate degradation of absolute trajectory error with increasing mirror duration,whilst the remaining results did not show significantly degraded localisation *** mesh maps generated proved to be very inaccurate,with real and virtual reflections colliding in the reconstructions.A discussion is given of the likely sources of error and robustness in mirror environments,outlining future directions for validating and improving vSLAM performance in the presence of planar *** MirrEnv dataset is available at https://***/10.17035/d.2023.0292477898.

关键词： visual simultaneous localisation and mapping(vSLAM) mirror localisation mapping reflection datase

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GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development

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Horticultural Plant Journal 2024年第1期10卷 223-238页

作者： Xinyue Liu Ling he ze Wu Nianjun Teng Key Laboratory of Landscaping Agriculture Ministry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Key Laboratory of Biology of Ornamental Plants in East China National Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Jiangsu Graduate Workstation of Nanjing Agricultural University and Nanjing Oriole Island Modern Agricultural Development Co. Ltd.NanjingJiangsu 210043China

Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination *** are many studies on pollen development in model plants,but few on flower crops such as ***(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive *** is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther ***,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in *** quantitative PCR results showed that LoMYB65 was mainly expressed in lily ***65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf *** conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its *** of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male ***,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen *** of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be ***,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in ***65 may provide a useful candidate gene for pollenless breeding of lily.

关键词： Lily Anther development Pollen pollution GAMYB VIGS

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Body composition and metabolic syndrome in patients with type 1 diabetes

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World Journal of Diabetes 2024年第1期15卷 81-91页

作者： Qiong zeng Xiao-Jing Chen Yi-Ting he ze-Ming Ma Yi-Xi Wu kun Lin Department of Neurology The First Affiliated Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Medical College Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Department of Endocrinology The First Affiliated Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina

BACKGROUND In recent years,the prevalence of obesity and metabolic syndrome in type 1 diabetes(T1DM)patients has gradually *** resistance in T1DM deserves *** is necessary to clarify the relationship between body composition,metabolic syndrome and insulin resistance in T1DM to guide clinical treatment and *** To assess body composition(BC)in T1DM patients and evaluate the relationship between BC,metabolic syndrome(MS),and insulin resistance in these *** A total of 101 subjects with T1DM,aged 10 years or older,and with a disease duration of over 1 year were *** impedance analysis using the Tsinghua-Tongfang BC Analyzer BCA-1B was employed to measure various BC *** and laboratory data were collected,and insulin resistance was calculated using the estimated glucose disposal rate(eGDR).RESULTS MS was diagnosed in 16/101 patients(15.84%),overweight in 16/101 patients(15.84%),obesity in 4/101(3.96%),hypertension in 34/101(33.66%%)and dyslip-idemia in 16/101 patients(15.84%).Visceral fat index(VFI)and trunk fat mass were significantly and negatively correlated with eGDR(both Pher body fat percentage and visceral fat ratio compared to male *** logistic regression analysis revealed that significant factors for MS included eGDR[P=0.017,odds ratio(OR)=0.109],VFI(P=0.030,OR=3.529),and a family history of diabetes(P=0.004,OR=0.228).Significant factors for hypertension included eGDR(Phe increased risk of

关键词： Body composition Metabolic syndrome Insulin resistance Visceral fat Estimated glucose disposal rate

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Atomic-scale mechanism of effect of co-separation of elements at interface of ferrite and austenite on Cr-depleted zone of duplex stainless steel

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Journal of Iron and Steel Research(International) 2024年第3期31卷 710-718页

作者： Yan-hui Hou ze-kun Xu Shi-long Zhou Guang-qiang Li The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education Wuhan University of Science and TechnologyWuhan 430081HubeiChina

Three-dimensional atom-probe tomography and first-principles calculation combined with density functional theory were used to study the effect of the co-segregation of different elements formed during the solidification process of S32205 duplex stainless steel on the Cr-depleted zone at the interface between ferrite and *** was found that the co-segregation of different elements formed during the solidification process of duplex stainless steel can also form Cr-depleted zone at the interface between ferrite and ***,Mo,Si,B,C and P atoms promote co-segregation with Cr atoms,which promotes the formation of Cr-depleted zone at the interface between ferrite and austenite in duplex stainless *** and Si strongly promote the segregation of Cr at the interface between ferrite and austenite,thereby promoting the formation of Cr-depleted zone.B,C and P elements also promote the segregation of Cr element at the interface between ferrite and austenite and the formation of Cr-depleted zone,but their effect is weaker than that of Mo and Si *** conclusions provide a new theoretical basis for improving the intergranular corrosion performance of duplex stainless steel.

关键词： Duplex stainless steel Cr-depleted zone Intergranular corrosion Co-segregation First-principles calculation

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Effect of multiple coulomb scattering on the beam tests of silicon pixel detectors

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Nuclear Science and Techniques 2024年第4期35卷 200-207页

作者： Lan-kun Li Ming-Yi Dong ze Gao Liang-Cheng-Long Jin Shu-Jun Zhao School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China State Key Laboratory of Particle Detection and Electronics(Institute of High Energy Physics CAS)Beijing 100049China University of Chinese Academy of Science Beijing 100049China University of South China Hengyang 421001China

In the research and development of new silicon pixel detectors,a collimated monoenergetic charged-particle test beam equipped with a high-resolution pixel-beam telescope is crucial for prototype verification and performance *** the beam energy is low,the effect of multiple Coulomb scattering on the measured resolution of the Device Under Test(DUT)must be considered to accurately evaluate the performance of the pixel chips and *** study aimed to investigate the effect of multiple Coulomb scattering on the measured resolution,particularly at low beam *** were conducted using Allpix^(2) to study the effects of multiple Coulomb scattering under different beam energies,material budgets,and telescope *** simulations also provided the minimum energy at which the effect of multiple Coulomb scattering could be *** with the results of a five-layer detector system tested with an electron beam at DESY,the simulation results were consistent with the beam test results,confirming the reliability of the simulations.

关键词： Silicon Pixel Detectors Beam Telescope Multiple Coulomb Scattering Spatial Resolution

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Ca_(2)MnO_(4)-layered perovskite modified by NaNO_(3)for chemical-looping oxidative dehydrogenation of ethane to ethylene

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Chinese Journal of Chemical Engineering 2024年第4期68卷 53-64页

作者： Weixiao Ding kun Zhao Shican Jiang Zhen Huang Fang he College of Chemistry and Bioengineering Guilin University of TechnologyGuilin 541006China Guangzhou Institute of Energy Conversion Chinese Academy of SciencesGuangzhou 510640China School of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094China

Chemical-looping oxidative dehydrogenation(CL-ODH)is a process designed for the conversion of alkanes into olefins through cyclic redox reactions,eliminating the need for gaseous O_(2).In this work,we investigated the use of Ca_(2)MnO_(4)-layered perovskites modified with NaNO_(3) dopants,serving as redox catalysts(also known as oxygen carriers),for the CL-ODH of ethane within a temperature range of 700-780℃.Our findings revealed that the incorporation of NaNO_(3) as a modifier significantly-nhanced the selectivity for-thylene generation from Ca_(2)MnO_(4).At 750℃and a gas hourly space velocity of 1300 h^(-1),we achieved an-thane conversion up to 68.17%,accompanied by a corresponding-thylene yield of 57.39%.X-ray photoelectron spectroscopy analysis unveiled that the doping NaNO_(3) onto Ca_(2)MnO_(4) not only played a role in reducing the oxidation state of Mn ions but also increased the lattice oxygen content of the redox ***,formation of NaNO_(3) shell on the surface of Ca_(2)MnO_(4) led to a reduction in the concentration of manganese sites and modulated the oxygen-releasing behavior in a step-wise *** modulation contributed significantly to the enhanced selectivity for ethylene of the NaNO_(3)-doped Ca_(2)MnO_(4) *** findings provide compelling evidence for the potential of Ca_(2)MnO_(4)-layered perovskites as promising redox catalysts in the context of CL-ODH reactions.

关键词： Chemical-looping oxidative dehydrogenation Ethane Ethylene NaNO_(3)-doped Ca_(2)MnO_(4)redox catalyst Layered perovskites

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S-(1,3-Dioxoisoindolin-2-yl)O,O-diethyl phosphorothioate (SDDP): A practical electrophilic reagent for the phosphorothiolation of electron-rich compounds

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Chinese Chemical Letters 2024年第5期35卷 151-154页

作者： ze-Yuan Ma Mei Xiao Cheng-kun Li Adedamola Shoberu Jian-Ping Zou Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry and Chemical EngineeringSoochow UniversitySuzhou 215123China

An efficient synthesis of the electrophilic reagent,S-(1,3-dioxoisoindolin-2-yl)O,O-diethyl phosphorothioate(SDDP)is ***,the synthetic applications of SDDP wherein the transfer of the SP(O)(OEt)_(2) moiety occurs were *** this manner,SDDP underwent facile SP(O)(OEt)_(2) transfer with electron-rich substrates such as ketones,indoles,and thiols to formα-phosphorothiolated ketones,3-phosphorothiolated indoles and S-phosphorothiolated thioethers,respectively.

关键词： Electrophilic reagent Phosphorothiolation Electrophilic substitution

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heat distribution model under hydrogen-rich low-carbon conditions in blast furnace

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Journal of Iron and Steel Research(International) 2024年第3期31卷 584-594页

作者： Gang Wang Jun Xu kun he Zhong-ping Zou Hao Bai School of Metallurgy and Ecological Engineering University of Science and Technology BeijingBeijing 100083China CISDI Low-Carbon Blast Furnace Research Office MCC Low-Carbon Technology Research InstituteChongqing 401122China

Low carbon development of blast furnaces is one of the key technological directions in the current development of *** to the differences in the physical and chemical properties of hydrogen and carbon,hydrogen-rich media entering a blast furnace will change the heat distribution,thus affecting the stability of ***,a heat distribution model was proposed to study the temperature distribution in a blast furnace,simultaneously considering gas-solid heat exchange,slag and iron melting,and chemical *** model was used to analyze the temperature distribution of a 2300 m^(3) blast furnace and was verified via comparison with actual production ***,the effects of the injection rate of hydrogen-rich media,H2 concentration,and oxygen enrichment rate of the blast on the temperature distribution were *** indicated that the increase in the injection rate of the hydrogen-rich media decreased the amount of direct reduction and led to an increase in the furnace ***,an increase in the oxygen enrichment rate led to a decrease in the furnace temperature,but could reduce the solid fuel ratio,while the change in H2 concentration had less effect on the temperature *** combination of hydrogen-rich media injection and the increase in the oxygen enrichment rate would help to adjust the temperature distribution to the same level as the conventional blast furnace conditions.

关键词： heat distribution Blast furnace Low-carbon condition Hydrogen enrichment Calculation model

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