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Induced neural stem cells regulate microglial activation through Akt-mediated upregulation of CXCR4 and Crry in a mouse model of closed head injury

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Neural Regeneration Research 2025年第5期20卷 1416-1430页

作者： Mou Gao qin Dong Dan zou Zhijun Yang Lili Guo Ruxiang Xu Department of Neurosurgery Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan ProvinceChina Department of Neurosurgery Chinese PLA General HospitalBeijingChina Department of Neurology Fu Xing HospitalCapital Medical UniversityBeijingChina

Microglial activation that occurs rapidly after closed head injury may play important and complex roles in neuroinflammation-associated neuronal damage and *** previously reported that induced neural stem cells can modulate the behavior of activated microglia via CXCL12/CXCR4 signaling,influencing their activation such that they can promote neurological ***,the mechanism of CXCR4 upregulation in induced neural stem cells remains *** this study,we found that nuclear factor-κB activation induced by closed head injury mouse serum in microglia promoted CXCL12 and tumor necrosis factor-αexpression but suppressed insulin-like growth factor-1 ***,recombinant complement receptor 2-conjugated Crry(CR2-Crry)reduced the effects of closed head injury mouse serum-induced nuclear factor-κB activation in microglia and the levels of activated microglia,CXCL12,and tumor necrosis factor-α.Additionally,we observed that,in response to stimulation(including stimulation by CXCL12 secreted by activated microglia),CXCR4 and Crry levels can be upregulated in induced neural stem cells via the interplay among CXCL12/CXCR4,Crry,and Akt signaling to modulate microglial *** agreement with these in vitro experimental results,we found that Akt activation enhanced the immunoregulatory effects of induced neural stem cell grafts on microglial activation,leading to the promotion of neurological recovery via insulin-like growth factor-1 secretion and the neuroprotective effects of induced neural stem cell grafts through CXCR4 and Crry upregulation in the injured cortices of closed head injury ***,these beneficial effects of Akt activation in induced neural stem cells were positively correlated with the therapeutic effects of induced neural stem cells on neuronal injury,cerebral edema,and neurological disorders post–closed head *** conclusion,our findings reveal that Akt activation may enhance the immunoregulatory effects of induced neura

关键词： Akt signaling cerebral edema closed head injury Crry CXCR4 induced neural stem cell microglia neuroinflammation

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基于Hurst指数的DNA序列相似性分析

西北农林科技大学学报（自然科学版） 2011年第3期39卷 17-21,27页

作者：刘晓邹克琴李素芳重庆大学通信工程学院重庆400044 中国计量学院生命科学学院浙江杭州310018

【目的】建立直接将Hurst指数作为指标参数进行DNA序列相似性分析的方法。【方法】以11个物种β-球蛋白第一个外显子的编码序列作为分析对象,首先将DNA序列转化为数字序列,然后用重标极差分析法计算各编码序列的全序列Hurst指数,之后利... 详细信息

【目的】建立直接将Hurst指数作为指标参数进行DNA序列相似性分析的方法。【方法】以11个物种β-球蛋白第一个外显子的编码序列作为分析对象,首先将DNA序列转化为数字序列,然后用重标极差分析法计算各编码序列的全序列Hurst指数,之后利用它们的Hurst指数构建距离矩阵进行相似性分析,并将建立的基于Hurst指数的DNA序列相似性分析方法与其他方法的分析结果进行比较。【结果】建立的基于Hurst指数的DNA序列相似性分析方法,较好地从相关性角度反映了分析序列的生物特性指标,具有较好的相似性分析效能。与其他方法相比,该方法分析结果的敏感性较高,有助于提高进化距离较近的分析对象间的区别度。【结论】Hurst指数可以作为指标参数描述DNA序列的相似性,可将其进一步应用于生物序列的信息分析。

关键词： Hurst指数外显子序列相似性

Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-Min qin Zhongliang Li Wen‑Xia Su Jia‑Min Hu Hanqin zou Zhixuan Wu Zhiqin Ruan Yue‑Peng Cai Kang Li Qifeng Zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China key Laboratory of Functional Metal‑Organic Compounds of Hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery

Fe^(2+)对紫背浮萍生长和生理生化特性的影响

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北方园艺 2012年第11期 15-18页

作者：徐晓晖林海芳邹克琴中国计量学院生命科学学院浙江杭州310018 中国计量学院现代科技学院浙江杭州310018

通过水培试验研究了不同浓度Fe2+处理下对紫背浮萍生长、光合色素含量、Fv/Fm值、过氧化物酶(POD)活性和丙二醛(MDA)含量的影响。结果表明:Fe2+在0.2～10mg/L浓度范围内,紫萍的生长、Fv/Fm、可溶性蛋白和MDA含量没有受到明显影响,光合... 详细信息

通过水培试验研究了不同浓度Fe2+处理下对紫背浮萍生长、光合色素含量、Fv/Fm值、过氧化物酶(POD)活性和丙二醛(MDA)含量的影响。结果表明:Fe2+在0.2～10mg/L浓度范围内,紫萍的生长、Fv/Fm、可溶性蛋白和MDA含量没有受到明显影响,光合色素含量和POD活性有所升高,表现出较强的抗性;Fe2+浓度超过20mg/L时开始对紫萍构成胁迫,使紫萍的生长抑制,光合色素含量、Fv/Fm、可溶性蛋白含量、POD活性下降,MDA含量升高,产生明显的毒性效应。

关键词： Fe2+ 紫背浮萍生理生化特性

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南瓜疏松愈伤组织诱导的研究

北方园艺 2007年第12期 201-203页

作者：邹克琴张拥军楼纪东丰金平曹恬中国计量学院生命科学学院浙江杭州310018

获得生长迅速,疏松的愈伤组织是进行植物细胞悬浮培养的前提。研究以"粟冠"南瓜子叶做外植体,在添加不同浓度的BA和NAA的MS固体培养基上诱导南瓜愈伤组织,比较南瓜愈伤组织的诱导率和长势,其中当外植体在MS+1.0mg/L6-BA+0.1mg/LNAA的培... 详细信息

获得生长迅速,疏松的愈伤组织是进行植物细胞悬浮培养的前提。研究以"粟冠"南瓜子叶做外植体,在添加不同浓度的BA和NAA的MS固体培养基上诱导南瓜愈伤组织,比较南瓜愈伤组织的诱导率和长势,其中当外植体在MS+1.0mg/L6-BA+0.1mg/LNAA的培养基上培养时,愈伤组织诱导率和长势最好。

关键词：南瓜愈伤组织诱导率

水稻WRKY转录因子OsWRKY71的cDNA分离和表达分析

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浙江农业学报 2014年第6期26卷 1405-1411页

作者：邹克琴汪得凯胡东维李素芳陈振煌中国计量学院生命科学学院浙江杭州310018 浙江省农业科学院浙江省植物有害生物防控重点实验室浙江杭州310021 浙江大学生物技术研究所浙江杭州310058

WRKY蛋白是植物中最大的转录因子家族之一,对植物的生长发育具有重要调控作用。利用SMART-RACE-PCR技术分离获得水稻WRKY转录因子基因(Os WRKY71)的全长c DNA序列,并进行了相关的生物信息学分析。分离到的水稻WRKY转录因子c DNA全长为1 ... 详细信息

WRKY蛋白是植物中最大的转录因子家族之一,对植物的生长发育具有重要调控作用。利用SMART-RACE-PCR技术分离获得水稻WRKY转录因子基因(Os WRKY71)的全长c DNA序列,并进行了相关的生物信息学分析。分离到的水稻WRKY转录因子c DNA全长为1 245 bp(Gen Bank登录号KJ137000),开放阅读框1 047 bp,编码348个氨基酸,分子量为38.28 k Da,Os WRKY71具有WRKY转录因子家族典型的保守结构域,属于第Ⅱ组WRKY蛋白家族。系统进化分析表明,Os WRKY71氨基酸序列与禾本科作物小麦的亲缘关系最近,其中与小麦序列相似性为69%,和大麦的序列相似性为68%。荧光定量PCR检测表明,OsWRKY71在孕穗期剑叶中表达丰度最高,根中最低,具有表达的空间差异性。抗病品种‘IR28’在受到稻曲菌诱导的初期,Os WRKY71基因表达呈现先升高后下降趋势,易感品种‘甬优9号’在受到稻曲菌诱导后表达受到抑制。这些结果表明,Os WRKY71的表达对稻曲菌侵染有应答响应,很可能在水稻防御稻曲菌侵染的机制中发挥作用。

关键词：水稻 WRKY转录因子表达分析实时定量PCR

Bifunctional bridging capping layer enables 24.5% efficiency of perovskite solar cells with polymer-based hole transport materials

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Science China Chemistry 2025年第1期 350-359页

作者： Can Zhu Yiyang Wang Lei Meng Beibei Qiu Jing Li Shucheng qin ke Hu Xin Jiang Wenbin Lai Minchao Liu Zhe Liu Chenxing Lu Jinyuan Zhang Yongfang Li Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences School of Chemical Science University of Chinese Academy of Sciences key Laboratory of Solid State Optoelectronic Devices of Zhejiang Province College of Physics and Electronic Information Engineering Zhejiang Normal University key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of Sciences Laboratory of Advanced Optoelectronic Materials College of Chemistry Chemical Engineering and Materials ScienceSoochow University

Developing a bridge capping layer between perovskite and hole transport layer materials（HTMs） in the n-i-p perovskite solar cells（pero-SCs） is an effective approach to modify the morphology of HTMs and passivate the perovskite ***, we select the quinoxaline-based bifunctional passivation agent, quinoxalin-6-yl-methylamine hydrochloride（Qx MACl）,as the bridging layer, and a D-A copolymer PBQ12 containing the same quinoxaline unit as an HTM for the n-i-p pero-SCs. Due to the π-π stacking among the common quinoxaline units in the bridge layer and HTM, Qx MACl induces the π-π stacking of the PBQ12 film and improves the film morphology of HTMs with better conductivity. Additionally, Qx MACl can effectively passivate the perovskite surface, and PBQ12 possesses appropriate energy levels and high hole mobility. The pero-SCs based on FAPbI3with PBQ12/Qx MACl treatment showed a higher power conversion efficiency（PCE） of 24.05% and outstanding stability, maintaining 95.4% and 92.1% of its initial PCE after 750 h of storage and after over 800 h of thermal annealing at 85 °C, respectively. To further enhance the PCE of the PBQ12/Qx MACl-based devices, we developed a non-metal ion dopant for the PBQ12 HTM. Through trace doping of PBQ12 HTM by the non-metal ion dopant, the PCE of the PBQ12/Qx MACl-based devices reached 25.24%（the calibrated PCE of 24.55% by the National Institute of Metrology, China）.

关键词：

同方期刊数据库

菰黑粉菌的分离鉴定及其发酵液中植物激素的检测

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菌物学报 2010年第2期29卷 178-184页

作者：尤文雨邹克琴俞晓平刘倩安欣欣赵煦泓叶子弘中国计量学院生命科学学院杭州310018

在显微镜下观察孢子形态,并观察单菌落的形态和菌株的微观形态,PCR扩增其ITS-5.8S rDNA序列,测序并在NCBI中进行同源比较,确定其种属。液体培养该菌株,通过高效液相色谱法检测发酵液中植物激素。结果表明:用菌落形态与孢子形态鉴定和分... 详细信息

在显微镜下观察孢子形态,并观察单菌落的形态和菌株的微观形态,PCR扩增其ITS-5.8S rDNA序列,测序并在NCBI中进行同源比较,确定其种属。液体培养该菌株,通过高效液相色谱法检测发酵液中植物激素。结果表明:用菌落形态与孢子形态鉴定和分子生物学鉴定的方法,对茭白中分离的一个菌株鉴定为菰黑粉菌,且在其发酵液中检测到植物激素IAA、ABA和GA3,其中IAA含量为0.1306mg/L,ABA含量为0.01367mg/L。

关键词：菰黑粉菌形态分子鉴定 HPLC

菰黑粉菌孢子萌发过程形态学观察及系统发育研究

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中国计量学院学报 2010年第2期21卷 140-145页

作者：李志兰尤文雨邹克琴叶子弘浙江省自然科学基金委员会浙江杭州310012 中国计量学院生命科学学院浙江杭州310018

通过显微观察法研究菰黑粉菌厚垣孢子在28℃、PDA液体培养基中静止萌发各时期的菌体形态,克隆了菰黑粉菌18S rDNA序列并测序,结合已有ITS-5.8S rDNA序列和18S rDNA序列分析其系统发育地位.结果表明,从厚垣孢子到形成稳定的担孢子需要62~... 详细信息

通过显微观察法研究菰黑粉菌厚垣孢子在28℃、PDA液体培养基中静止萌发各时期的菌体形态,克隆了菰黑粉菌18S rDNA序列并测序,结合已有ITS-5.8S rDNA序列和18S rDNA序列分析其系统发育地位.结果表明,从厚垣孢子到形成稳定的担孢子需要62~72 h,经历厚垣孢子萌发、芽管形成、先菌丝形成、担孢子和小孢子等几个阶段,在体外培养时多以棒状担孢子形态存在,培养30 d后,在培养基表面或边缘形成白色丝状菌丝;系统发育分析结果显示菰黑粉菌与Ustilago属和Sporisorium属亲缘关系较近,与黑粉菌属中的Ustilago triodiae亲缘关系最近.

关键词：菰黑粉菌孢子萌发过程形态学