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应用型公共卫生人才核心胜任力指标体系研究

中国公共卫生管理 2024年第3期40卷 361-365页

作者：杨莉萍张涛邹泉陈佳慎王长义石磊深圳市南山区慢性病防治院广东深圳518054 深圳市疾病预防控制中心广州医科大学卫生管理学院

目的构建应用型公共卫生人才核心胜任力评价指标体系,为我国公共卫生人才培养模式探索提供思路。方法采用文献研究与政策文本分析、半结构式访谈等研究方法初步构建指标体系,运用德尔菲法最终确定指标体系及其权重。结果来自医学院校、... 详细信息

目的构建应用型公共卫生人才核心胜任力评价指标体系,为我国公共卫生人才培养模式探索提供思路。方法采用文献研究与政策文本分析、半结构式访谈等研究方法初步构建指标体系,运用德尔菲法最终确定指标体系及其权重。结果来自医学院校、疾病预防控制中心、慢性病防治院的18名专家参与咨询,最终确定指标体系由4项一级指标、10项二级指标及68项三级指标构成。结论构建的应用型公共卫生人才核心胜任力指标体系具有科学性和可靠性,可为公共卫生人才培养、评估与考核提供参考依据,也可为公共卫生教育事业发展提供新方向。

关键词：应用型公共卫生胜任力指标体系

Upreguiation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells

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World Journal of Gastroenterology 2005年第36期11卷 5627-5632页

作者： Zhen-Liang Qu Sheng-quan zou Nai-Qiang Cui Xian-Zhong Wu Ming-Fang Qin Di Kong Zhen-Li Zhou Department of surgery Tianjin Nankai Hospital Tianjin 300100 China Department of Surgery Tongji Hospital Wuhan430030 Hubei Province China

AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis. METHODS： HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein （EGFP） coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection. Flow cytometry was performed to determine the transfection efficiency. Cells were harvested and total RNA was extracted using TRIzol reagent. The expression of hTERT mRNA in HepG2 and QBC939 cell lines was assayed by reverse transcriptionpolymerase chain reaction. The expression of HBx protein in both cell lines was detected by immunocytochemical staining and Western blotting. RESULTS： Flow cytometry showed that the transfection efficiency was 46.4% in HepG2 cells and 29.6% in QBC939 cells for both HBx gene expression vector and blank vector. The expression of hTERT mRNA was meaningfully increased in HepG2 and QBC939 cell lines when transfected with HBx gene expression vector compared to those transfected with OPTI-MEM medium and blank vector. Immunocytochemical staining and Western blotting revealed HBx protein expression in HepG2 and QBC939 cells only when transfected with HBx gene. CONCLUSION： HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA. The transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection. 2005 The WJG Press and Elsevier Inc. All rights reserved

关键词： Hepatocholangiocarcinoma Human telomerasereverse transcriptase Gene expression Hepatitis B virus X protein

Multicomponent cross coupling via synergistic photoredox and copper catalysis

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Science Bulletin 2020年第18期65卷 1516-1518,M0003页

作者： Fen Tan You-quan zou Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients Hubei University of EducationWuhan 430205China Department of Chemistry University of CambridgeCambridge CB21EWUK

Bicyclo[1.1.1]pentane (BCP) scaffolds can serve as bioisosteres of phenyl, tert-butyl and alkyne groups which are widely used in medicinal chemistry today [1]. Replacing para-phenyl rings in most of pharmaceutical candidates with 1,3-disubstituted BCP moieties will improve their biological activities and physico-chemical profiles[2].

关键词： catalysis synergistic coupling

Effects of targeting magnetic drug nanoparticles on human cholangiocarcinoma xenografts in nude mice

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Hepatobiliary & Pancreatic Diseases International 2007年第3期6卷 303-307页

作者： Tang, Tao Zheng, Jian-Wei Chen, Bo Li, Hong Li, Xi Xue, Ke-Ying Ai, Xing zou, Sheng-quan Huazhong Univ Sci & Technol Tongji Med Coll Dept Gen Surg Tongji Hosp Wuhan 430030 Peoples R China Huazhong Univ Sci & Technol Tongji Med Coll Dept Resp Dis Tongji Hosp Wuhan 430030 Peoples R China Huazhong Univ Sci & Technol Tongji Med Coll Dept Urinary Surg Tongji Hosp Wuhan 430030 Peoples R China Wuhan Univ Renming Hosp Dept Gastroenterol Wuhan 430030 Peoples R China

BACKGROUND: Targeting is a new therapeutic tool for malignant tumor as a result of combining nanotechnology with chemotherapeutics. The aim of our study was to investigate the effects of magnetic nanoparticles enveloping a chemotherapeutic drug on human cholangiocarcinoma xenografts in nude mice. METHODS: The human cholangiocarcinoma xenograft model was established in nude mice with the QBC939 cell line. The nude mice were randomly assigned to 7 groups. 0.9% saline or magnetic nanoparticles, including high (group 2), medium (group 4) and low (group 5) dosages, were given to nude mice through the tail vein 20 days after the QBC939 cell line was implanted. Calculations were made 35 days after treatment in order to compare the volumes, inhibition ratios and growth curves of the tumors in each group. Mice in each group were sacrificed randomly to collect tumor tissues and other organs for electron microscopy and pathological examination. RESULTS: The high and medium dosage groups were significantly different from the control group (P<0.05). The tumor inhibition ratios for the high, medium and low dosage groups were 39.6%, 14.6% and 7.9%, respectively. The tumor growth curve of groups 5, 4, and 2 changed slowly in turn. The high and medium groups showed cell apoptosis under an electron microscope. CONCLUSION: Magnetic nanoparticles can inhibit the growth of human cholangiocarcinoma xenografts in nude mice.

关键词： magnetic nanoparticles targeting therapy nude mice cholangiocarcinoma

The Inventory Demand Forecasting Model of the Regional Logistics Network in Supply Chain

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The Inventory Demand Forecasting Model of the Regional Logis...

Synthesis of Ultra-High-Molecular-Weight Polyethylene by Transition-Metal-Catalyzed Precipitation Polymerization

2012 IEEE 19th International Conference on Industrial Engineering and Engineering Management(IE&EM 2012)

作者： zou An-quan Huang Ren-Cun Department of Business Administration Changsha University Changsha Institute of Logistics Technology and Application

This article is conducting a study on the demand forecast from the x-retailers,y-distribution centers,z-suppliers supply chain of logistics area. The supply chain will be divided into upstream and downstream levels. Combined with the characteristics of the supply chain and demand characteristics of the regional logistics network, Dijkstra shortest path algorithm is used and its demand forecast model is constructed based on it to obtain the exact demand of each node enterprises. At last, an examples is given to prove it.

关键词： Exoskeleton arm hybrid fuzzy control pneumatic force-feedback teleoperation

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Precision Chemistry 2024年第2期2卷 63-69页

作者： Wenbing Wang quan Wang Chen zou Changle Chen Key Laboratory of Precision and Intelligent Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026China

Ultra-high-molecular-weight polyethylene(UHMWPE)plays an important role in many important fields as engineering *** this contribution,a precipitation polymerization strategy is developed by combination of highly active phosphino-phenolate nickel catalysts with polymer-insoluble solvent(heptane)to access UHMWPE(Mn up to 8.3×10^(6)g mol^(-1))with good product morphology,free-flowing characteristics,and great mechanical *** with the academically commonly used aromatic solvent(toluene),the utilization of heptane offers simultaneous enhancement in important parameters including activity,polymer molecular weight,and catalyst thermal *** system can also generate polar functionalized UHMWPE with molecular weight of up to 1.6×10^(6)g mol^(-1)in the copolymerization of ethylene with polar *** importantly,this precipitation polymerization strategy is generally applicable to several representative transition metal catalyst systems,leading to UHMWPE synthesis with good product morphology control.

关键词： transition metal catalysts polyolefin UHMWPE precipitation polymerization polar monomer

一种基于贝叶斯理论的电站模拟量数据融合诊断方法

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中国电机工程学报 2006年第19期26卷 117-120页

作者：朱小良邹泉东南大学动力工程系江苏省南京市210096

火电站热工测量系统中,大量的模拟量数据以隐含冗余的方式存在。它们在测量时表现出独立性,内涵则通过对象关联。据此,提出了一种基于贝叶斯理论的电站模拟量数据融合诊断方法,推导了方差σ为已知和未知二种情况下测量模型系数的计算公... 详细信息

火电站热工测量系统中,大量的模拟量数据以隐含冗余的方式存在。它们在测量时表现出独立性,内涵则通过对象关联。据此,提出了一种基于贝叶斯理论的电站模拟量数据融合诊断方法,推导了方差σ为已知和未知二种情况下测量模型系数的计算公式,并从中得出结论:结合数据分布规律对其残差进行融合,可诊断热工测量数据。以汽机的功率测量为例,对某300MW火电机组实际测量数据进行诊断;结果表明,基于贝叶斯理论的数据融合诊断方法可直接诊断出异常的数据。

关键词：热能动力工程火电机组数据融合诊断贝叶斯理论模拟量

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南山区脑卒中患者住院费用的影响因素分析

预防医学 2024年第4期36卷 328-332,337页

作者：邹泉赵信星陈洪恩吴兰兰梁晓峰吴静王长义深圳市南山区慢性病防治院心脑血管与糖尿病防治科广东深圳518000 菏泽市疾病预防控制中心山东菏泽274000 暨南大学广东广州510632 中国疾病预防控制中心北京100050

目的分析不同亚型脑卒中患者住院费用的影响因素,为减轻患者经济负担提供参考。方法通过深圳市南山区各区属医院医疗信息系统收集2016年1月1日—2021年12月31日出院、主要诊断为出血性脑卒中和缺血性脑卒中的患者资料,分析不同亚型脑卒... 详细信息

目的分析不同亚型脑卒中患者住院费用的影响因素,为减轻患者经济负担提供参考。方法通过深圳市南山区各区属医院医疗信息系统收集2016年1月1日—2021年12月31日出院、主要诊断为出血性脑卒中和缺血性脑卒中的患者资料,分析不同亚型脑卒中患者住院费用;采用结构方程模型分析不同亚型脑卒中患者住院费用的影响因素。结果收集10298例脑卒中患者资料,其中出血性脑卒中2820例,占27.38%,住院时间M(Q_(R))为19.00(18.00)d,住院费用M(Q_(R))为26759.48元(51000.87)元;缺血性脑卒中7478例,占72.62%,住院时间M(Q_(R))为12.00(10.00)d,住院费用M(Q_(R))为12199.87(13290.20)元。结构方程模型分析结果显示,住院科室、出院时情况、离院方式、手术治疗和高血压既可直接影响出血性脑卒中患者住院费用,又可通过住院时间产生间接影响;总效应最大为住院时间(0.684),其次为手术治疗(0.632)。就业情况、入院途径、住院科室、离院方式、付款方式、手术治疗和血脂异常既可直接影响缺血性脑卒中患者住院费用,又可通过住院时间产生间接影响;总效应前三位依次为住院时间(0.746)、手术治疗(0.424)和住院科室(0.151)。结论南山区脑卒中患者住院费用相对较高,住院时间和手术治疗对不同亚型脑卒中患者住院费用影响较大。

关键词：脑卒中出血性脑卒中缺血性脑卒中住院费用影响因素

A novel strategy to prepare LDH networks loaded carbon structure by C-MEMS techniques for glucose detection

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Chinese Chemical Letters 2017年第1期28卷 149-152页

作者： Bo Hai Ying-quan zou Gui-Bao Guo Zheng-De Wang Yun-Ying Liu Xiao-Xia Wang Hui Yan Li-Tong Ma Yu-Chen Bai School of Chemistry and Chemical Engineering Inner Mongolia University of Science & Technology College of Chemistry Beijing Normal University

NiAl-layered double hydroxide（NiAl-LDH） networks loaded carbon microcylinder（CMC） hybrid was synthesized for the first time using typical carbon based microelectromechanical systems（C-MEMS）techniques combined with in situ growth progress. The incorporation of NiAl-LDH on C-MEMS structures via a simple pyrolysis of modified photoresist was investigated. With proper control of parameters in lithography and hydrothermal processes, the NiAl-LDH/CMC composites with suitable morphology were fabricated. When the composites applied as new catalytic material for glucose detection, this simple sensor showed satisfying electrocatalytic properties towards glucose oxidation owing to its unique structure and excellent electric conductivity. It is also worth pointing out that this novel fabrication process can equip carbon microfeatures with various nanostructures, and have wide potential applications in scaling up carbon based nanocomposites.

关键词： Lithography Incorporation C-MEMS techniques NiAl-layered double hydroxide Sensor