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Synergistic effect of oxymatrine and angiogenesis inhibitor NM-3 on modulating apoptosis in human gastric cancer cells

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World Journal of Gastroenterology 2007年第12期13卷 1788-1793页

作者： Ming-Quan Song jin-Shui zhu jin-Lian Chen long Wang Wei Da Li zhu Wei-Ping Zhang Department of Gastroenterology The Sixth Affiliated Hospital of Shanghai Jiaotong University Shanghai 200233 China

AIM: To investigate the synergistic effect of oxymatrine(OM) and angiogenesis inhibitor NM-3 on modulatingapoptosis in human gastric cancer cell lines SGC-7901,MKN-45, MKN-74. METHODS: Human gastric cancer lines SGC-7901,MKN-45, MKN-74 were treated with OM in the absenceand presence of NM-3. The inhibitory rates weredetected by MTT assay. Synergistic effect of OM andNM-3 on the growth of survivin, bcl-2, bax and p53 inSGC-7901 cells were examined by semiquantitative RT-PCR and Western blotting, and their growth inhibitoryeffects were also observed on SGC-7901 tumor xenograftin nude ***: OM combined with NM-3 exhibited asynergistic inhibitory effect on the growth of SGC-7901,MKN-45 and MKN-74 cells in a time-dependent ***-four hours after treatment with OM, NM-3 aloneand their combination, mRNA expression of survivin andbcl-2 in SGC-7901 cells decreased, p53 mRNA expressionincreased. OM (4 g/L) combined with NM-3 significantlyincreased the expression of p53 mRNA and decreasedthe expression of survivin and bcl-2 compared witheither agent alone (193% ± 34% vs 129% ± 12%;44% ± 18% vs 92% ± 18%; 36 ± 17% vs 93% ± 23%,P < 0.05). Western blotting showed that the synergisticeffect of OM and NM-3 on protein translation of survivin,bcl-2 and p 53 was in accordance with their ***, OM/NM-3 combination obviously exhibitedantitumor growth effect in xenografted human gastriccancer cells SGC-7901 compared with either agent ***: OM combined with NM-3 has synergisticinhibitory effects on human gastric cancer cells in vitro and can suppress the growth of xenografted human gastric cancer cells SGC-7901 in vivo.

关键词： NM-3 compound Oxymatrine Gastric cancer Apoptosis

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A callus transformation system for gene functional studies in soybean

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Journal of Integrative Agriculture 2017年第9期16卷 1913-1922页

作者： XU Kun ZHANG Xiao-mei FAN Cheng-ming CHEN Fu-lu zhu jin-long ZHANG Shi-long CHEN Qing-shan FU Yong-fu Key Lab of Soybean Biology(Beijing) Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Bijie Agricultural Science Research Institute of Guizhou Bijie 551700P.R.China College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China

Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants（e.g., soybean）, which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies.

关键词： soybean callus gene function studies transformation

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Novel 6-bp Deletion Mutation in visfatin Gene and Its Associations with Birth Weight and Bodyweight in Chinese Cattle

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Journal of Integrative Agriculture 2012年第8期11卷 1327-1332页

作者： WANG Mou ZHANG Ya YU Hui LAI Xin-sheng zhu jin-long JIAO jin-zhen LAN Xian-yong LEI Chu-zhao ZHANG Liang-zhi CHEN Hong Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100P.R.China

Visfatin, like insulin, induces phosphorylation of signal transduction proteins that operatate downstream of the insulin receptor. The present study is focused on detecting deletion of visfatin gene and analyzing its effect on growth traits in six Chinese cattle breeds （Nangyang, Luxi, Qinchuan, Jiaxian Red, Grassland Red, and Chinese Holstein） using DNA sequencing, PCR-SSCP and PCR-RFLP methods. For the first time, a 6-bp deletion of visfatin was described and two alleles were revealed：W and D. The χ2-test analysis demonstrated that all breeds were in agreement with Hardy-Weinberg equilibrium （P〉0.05）. The associations of the novel 6-bp deletion of visfatin gene with growth traits of Nanyang cattle at 6-, 12-, 18-, and 24-mon-old were analyzed. Birth weight, 12- and 24-mon-old cattle with genotype WW had greater birth weight and average daily gain than genotype WD （P〈0.01 or P〈0.05）. These results suggest that the deletion may influence the birth weight and bodyweight in 12 mon-old cattle.

关键词： visfatin deletion mutation cattle

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Late Paleozoic to early Mesozoic volcanic-sedimentary history of the southeastern margin of the Central Asian Orogenic Belt: Timing of uplift of the Inner Mongolia paleo-uplift

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Science China Earth Sciences 2025年第1期68卷 297-309页

作者： Wenxing HAO jin-Hui YANG Guang zhu State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences School of Resource and Environmental Engineering Hefei University of Technology

The Kulun region is located in the eastern Bainaimiao arc belt on the southeastern margin of the Central Asian Orogenic Belt(CAOB), adjacent to the North China Craton(NCC) to the south. This region records the closure of the PaleoAsian Ocean during the Paleozoic to early Mesozoic and is key to study the tectonic evolution of the northern margin of the *** conducted zircon U-Pb geochronology and Hf isotope analysis of volcanic-sedimentary and intrusive rocks in the Kulun region. The volcanic-sedimentary rocks were previously assigned an early Carboniferous age, but include volcanic rocks with ages of 267–266 Ma and sedimentary rocks with youngest detrital zircon age peaks of 265–244 Ma, which were intruded by a granite with an age of 242 Ma. This indicates that these rocks are actually middle Permian to Middle Triassic in age(267–242Ma) and correspond to the Qingfengshan and Liujiagou formations. Moreover, two volcanic rock samples have high εHf(t) values(+1.6 to +16.1) and relatively young two-stage Hf model ages(1095–327 Ma), indicating their sources associated with the CAOB. The age spectra and Hf isotopic characteristics of detrital zircons from the sedimentary rocks suggest that, during the middle Permian to Middle Triassic, the sedimentary provenance in the Kulun region was mainly the CAOB. However, by the Early Jurassic, the provenance changed to the NCC(i.e., the Inner Mongolia Paleo-uplift, IMPU). This result, combined with the development of a series of conglomerates on the northern margin of the NCC in the Late Triassic(i.e., Xingshikou, Xiaoyingzi,and Xiaohekou formations), indicates the Kulun region experienced an abrupt change in paleogeography during the Late Triassic, with the IMPU undergoing significant uplift and thus becoming the main sediment source in the Kulun region. Based on the Late Triassic alkaline igneous belt(235–215 Ma) in the IMPU, we speculate that this uplift was caused by detachment of the southward-subducting Paleo-Asian oceanic

关键词： Central Asian Orogenic Belt the North China Craton Zircon geochronology Hf isotopes Inner Mongolia Paleo-uplift

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水合物研制、结构与性能及其在能源环境中的应用

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物理学报 2019年第1期68卷 107-122页

作者：朱金龙赵予生靳常青北京高压科学研究中心北京100094 南方科技大学物理系深圳518055 中国科学院物理研究所北京100190

天然气水合物是与能源和环境相关的物质,可以进行甲烷等能源气体的存储和提取,也可以用于对二氧化碳等废气的封存.天然气水合物主要分为三种结构:sI, sII和sH,在本文中对其稳定性、水笼类型和大小以及可俘获气体进行了论述.中子衍射技... 详细信息

天然气水合物是与能源和环境相关的物质,可以进行甲烷等能源气体的存储和提取,也可以用于对二氧化碳等废气的封存.天然气水合物主要分为三种结构:sI, sII和sH,在本文中对其稳定性、水笼类型和大小以及可俘获气体进行了论述.中子衍射技术是研究水合物的重要手段之一,有着独特的优势.如中子的穿透性可以研究在高压状态下压力腔体内的大块样品;中子对于轻元素的敏感性可以很好地确定水合物当中的碳、氢、氧元素.通过中子衍射和非弹散射可以得到水合物中H/D原子的位置、各向异性振动因子、不同温度压力下的客体分子的水笼占据率、客体分子在水笼中的无序分布、原子核密度分布(通过最大熵方法);通过时间分辨中子,可以检测水合物形成及分解过程的热力学和动力学过程.而利用非弹中子可以得到气体分子平移和旋转振动模式以及分子的量子态转变.通过二氧化碳气体注入对天然气水合物的开采可以实现能源气体甲烷的开采和废气二氧化碳的水合物封存,在减小地质灾害和开采成本上有着独特的优势.

关键词：水合物高压中子衍射 CO2注入

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Structural and Electrical Characters of Ba_(0.6)Sr_(0.4)TiO_3/La_(0.5)Sr_(0.5)CoO_3 Thin Films by Plus Laser Deposition

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Journal of Electronic Science and Technology of China 2007年第4期5卷 303-307页

作者： Wen-Feng Qin Jun zhu Jie Xiong jin-long Tang Xiao Feng State Key Laboratory of Electronic Thin Film and Integrated Devices University of Electronic Science and Technology of China （UESTC） Chcngdu 610054 China

Epitaxial Ba0.6Sr0.4TiO3 （BST） thin films were deposited on LaAlO3 （LAO） substrates with the conductive metallic oxide La0.5 Sr0.5CoO3 （LSCO） film as a bottom electrode by pulsed laser deposition （PLD）. X-ray diffraction 0-20 andФ scan showed that the epitaxial relationship of BST/LSCO /LAO was [001] BST// [001] LSCO//[001] LAO. The atomic force microscope （AFM） revealed a smooth and crack-free surface of BST films on LSCO-coated LAO substrate with the average grain size of 120 nm and the RMS of 1.564 nm for BST films. Pt/BST/LSCO capacitor was fabricated to perform Capacitance-Voltage measurement indicating good insulating characteristics. For epitaxial BST film, the dielectric constant and dielectric loss were determined as 471 and 0.03, respectively. The tunabilty was 79.59% and the leakage current was 2.63×10^-7 A/cm^2 under an applied filed of 200 kV/cm. Furthermore, it was found that epitaxial BST （60/40） fdms demonstrate well-behaved ferroelectric properties with the remnate polarization of 6.085μC/cm^2 and the coercive field of 72 kV/cm. The different electric properties from bulk BST （60/40） materials with intrinsic paraelectric characteristic are attributed to the interface effects.

关键词： BST electrode LSCO thin film.

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Recent advances of metal vacancies in energy and environmental catalysis:Synthesis,characterization,and roles

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Green Energy & Environment 2025年第1期10卷 84-108页

作者： long Sun Shunzheng Zhao Sirui Gao Ronghui zhu Yiran Tan Xiaolong Tang Honghong Yi School of Energy and Environmental Engineering University of Science and Technology BeijingBeijing100083China Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants Beijing100083China

With the ongoing depletion of fossil fuels,energy and environmental issues have become increasingly critical,necessitating the search for effective ***,being one of the hallmarks of modern industry,offers a promising avenue for ***,the question of how to significantly enhance the performance of catalysts has gradually drawn the attention of *** engineering,a commonly employed and effective approach to improve catalyst activity,has become a significant research focus in the catalysis field in recent *** vacancies have received extensive attention due to their simple ***,exploration of metal vacancies has remained stagnant for a considerable period,resulting in a scarcity of comprehensive reviews on this ***,based on the latest research findings,this paper summarizes and consolidates the construction strategies for metal vacancies,characterization techniques,and their roles in typical energy and environmental catalytic ***,it outlines potential challenges in the future,aiming to provide valuable references for researchers interested in investigating metal vacancies.

关键词： Metal vacancies Catalysis Construction strategies Characterization techniques Roles

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利用域排列算法设计铌酸锂晶体实现3μm中红外波段频域纯态单光子源

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物理学报 2022年第20期71卷 75-81页

作者：张晨涛石小涛朱文新朱金龙郝向英金锐博武汉工程大学光学信息与模式识别湖北省重点实验室武汉430205

中红外波段的单光子源对于下一代量子传感、量子通信和量子成像的研究非常重要.目前常用的产生中红外单光子源的方法是基于周期极化铌酸锂(periodically poled lithium niobate,PPLN)自发参量下转换过程.但是,基于普通PPLN制备的单光子... 详细信息

中红外波段的单光子源对于下一代量子传感、量子通信和量子成像的研究非常重要.目前常用的产生中红外单光子源的方法是基于周期极化铌酸锂(periodically poled lithium niobate,PPLN)自发参量下转换过程.但是,基于普通PPLN制备的单光子源频域纯度不高,最高值只有0.82左右,会影响量子信息处理方案的保真度.本文利用域设计理论对30 mm长铌酸锂晶体的4000个域进行了定制化排列,消除了相位匹配函数中的旁瓣,获得了高斯型的分布.计算得到的单光子源的频域纯度可达0.99,可调谐范围为2.7—3.3μm.该定制极化铌酸锂(customized poled lithium niobate,CPLN)有望为中红外波段量子信息研究提供性能优异的单光子光源.

关键词：铌酸锂晶体极化周期设计中红外波段量子光源

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苏州市483起已破命案的法医学回顾分析

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法医学杂志 2013年第1期29卷 31-33,36页

作者：高冬朱金龙苏州市公安局刑警支队江苏苏州215131

目的通过总结苏州市已破命案的案例资料,研究命案特征及其共性,分析命案现场的法医学要点。方法收集苏州市公安局2006年1月至2010年3月已破命案资料共483起,对数据资料进行分析。结果受害人和犯罪嫌疑人多为1人,均以男性、青壮年、外埠... 详细信息

目的通过总结苏州市已破命案的案例资料,研究命案特征及其共性,分析命案现场的法医学要点。方法收集苏州市公安局2006年1月至2010年3月已破命案资料共483起,对数据资料进行分析。结果受害人和犯罪嫌疑人多为1人,均以男性、青壮年、外埠人为主。犯罪嫌疑人多为故意杀人,以激情犯罪为主,口角纠纷最为常见。致伤工具以锐器为主,多为随身携带。死亡原因以机械性窒息和机械性损伤最为常见。结论苏州市命案在涉案人员社会学特点、作案动机、致伤工具、损伤分布、死亡原因、死亡方式等方面均反映出一定的共性,为今后命案侦破提供了经验。

关键词：法医病理学杀人案例分析

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入射光照对典型光刻胶纳米结构的光学散射测量影响分析

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物理学报 2020年第3期69卷 38-47页

作者：董正琼赵杭朱金龙石雅婷湖北工业大学湖北省质量工程重点实验室武汉430068 华中科技大学数字制造装备与技术国家重点实验室武汉430074

作为一种快速、低成本和非接触的测量手段,光学散射测量在半导体制造业中的纳米结构三维形貌表征方面获得了广泛关注与运用.光学散射测量是一种基于模型的测量方法,在纳米结构待测参数的逆向提取过程中,为降低参数之间的耦合性,通常需... 详细信息

作为一种快速、低成本和非接触的测量手段,光学散射测量在半导体制造业中的纳米结构三维形貌表征方面获得了广泛关注与运用.光学散射测量是一种基于模型的测量方法,在纳米结构待测参数的逆向提取过程中,为降低参数之间的耦合性,通常需要将结构的光学常数作为固定的已知量,即假设结构的材料光学常数不受光学散射仪入射光照的影响.事实上,这一假设对于半导体制造业中的绝大多数材料是成立的,但某些感光材料的光学常数有可能随着入射光的照射时间增加而发生改变,而由此产生的误差会在一定程度上传递给待测形貌参数的逆向提取值.本文针对聚甲基丙烯酸甲酯光刻胶薄膜培片和光栅结构分别开展了光学散射测量实验与仿真研究,结果表明该光刻胶材料的光学常数随着入射光照时间增加而变化,进而导致光栅结构形貌参数的提取结果较大地偏离于真实值,不容被忽视.这一研究发现将为更进一步提高光刻胶纳米结构三维形貌参数的测量精确度提供理论依据.

关键词：光学散射测量光刻胶纳米结构聚甲基丙烯酸甲酯光学常数

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