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Fluorescence characteristic changes of dissolved organic matter during municipal solid waste composting

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Journal of Environmental Sciences 2005年第6期17卷 953-956页

作者： WEI zi-min XI Bei-dou WANG Shi-ping XU Jing-gang zhou Yu-yan LIU hong-liang Chinese Research Academy of Environmental Sciences Beijing 100012 China Northeast Agricultural University Harbin 150030 China China Agricultural University Beijing 100083 China

Dissolved organic matter（DOM） of municipal solid waste（MSW） consists of minerals, water, ash and humic substances, and is known to enhance plant growth. In this study, inoculating microbes（Z J, MS） were used in municipal solid wastes composting, and composting implemented a industrialized technology. During composting, dissolved organic matter was extracted from the compost and purified. The spectral characteristics of dissolved organic matter was determined by fluorescence emission, excitation, and synchronous spectroscopy. Fluorescence emission, excitation, and synchronous spectra characterized by different relative fluorescent intensities and peaks over time. Fluorescence spectra were similar to that of fulvic acid in sewage sludge, indicating the presence of dissolved organic matter with aromatic structures and a high degree of molecular polymerization. Compared with the controls with no microbial inoculation, the microbe-inoculated treatments exhibited the increase of aromatic polycondensation, in the following order： MS ＋ ZJ 〉 ZJ 〉 MS 〉 CK.

关键词： MSW composting dissolved organic matter(DOM) inoculating microbes fluorescence spectra

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有机金属卤化物钙钛矿CH_(3)NH_(3)PbBr_(3)的各向异性Rashba效应

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原子与分子物理学报 2025年第6期42卷 153-158页

作者：蔡锦鸿余达明周贤中广东工业大学集成电路学院广州510006

本研究通过密度泛函理论结合全相对论赝势对钙钛矿CH_(3)NH_(3)PbBr_(3)中的各向异性Rashba效应进行了深入研究.发现,Pb-Br八面体的畸变引起了导带最小间隙的三重态在自旋轨道耦合(SOC)效应和晶格形变下的分裂,导致在不同方向上呈现出... 详细信息

本研究通过密度泛函理论结合全相对论赝势对钙钛矿CH_(3)NH_(3)PbBr_(3)中的各向异性Rashba效应进行了深入研究.发现,Pb-Br八面体的畸变引起了导带最小间隙的三重态在自旋轨道耦合(SOC)效应和晶格形变下的分裂,导致在不同方向上呈现出强烈的各向异性.Rashba带分裂的最大系数可达2.0 eV?,并通过分析能级和电荷密度分布解释了Rashba效应的形成机制.这些研究结果有助于深化对CH_(3)NH_(3)PbBr_(3)性质的理解,同时为其在自旋电子学领域的潜在应用提供了理论基础.

关键词：钙钛矿 CH_3NH_3PbBr_3 密度泛函理论自旋轨道耦合

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重组人促性腺激素-铜绿假单胞菌外毒素A蛋白的可溶性表达、纯化和对肿瘤细胞的抑制活性

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山东科学 2013年第2期26卷 41-47页

作者：郭凯赵晓燕周红姿陈凯李纪顺陈泉杨合同山东省科学院中日友好生物技术研究中心山东省应用微生物重点实验室山东济南250014

以人促性腺激素-铜绿假单胞菌外毒素A衍生物(GnRH-PE39KDEL)为研究对象,根据大肠杆菌密码子偏好性优化,通过基因合成的方法获得重组蛋白核酸序列,连接至pET-24b载体中,并转化入大肠杆菌BL21(DE3)及BL21(DE3)plyS中进行诱导表达。结果显... 详细信息

以人促性腺激素-铜绿假单胞菌外毒素A衍生物(GnRH-PE39KDEL)为研究对象,根据大肠杆菌密码子偏好性优化,通过基因合成的方法获得重组蛋白核酸序列,连接至pET-24b载体中,并转化入大肠杆菌BL21(DE3)及BL21(DE3)plyS中进行诱导表达。结果显示,在含有2 mg/mL葡萄糖的LB培养基中37℃培养至OD600约为0.6 h,加入0.2 mmol/L IPTG在30℃诱导2 h后,重组蛋白可以实现可溶性高表达。工程菌经超声波破碎、高速离心后,经镍柱纯化和脱盐后得到重组蛋白,蛋白纯度达到95%以上,蛋白最终得率在2.6 mg/g菌体。重组蛋白经IC50检测,可以较好地抑制肿瘤细胞株的生长。

关键词：人促黄体激素释放激素绿脓杆菌外毒素A 可溶性表达表达菌株

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In situ polymerization of poly(3,4-ethylenedioxythiophene)protective layer towards stable zinc anode

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Nano Research 2025年第1期18卷 382-394页

作者： Debin Luo Peng zhou Xiaowei Lv Panpan Sun Xiaohua Sun Hewei Zhao College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion MaterialsChina Three Gorges UniversityYichang 443002China Hubei Three Gorges Laboratory Yichang 443007China School of Chemistry Beihang UniversityBeijing 100191China

Aqueous zinc-ion devices are considered promising candidates for energy storage due to their high safety,low cost and relatively high energy ***,the dendrite growth,hydrogen evolution reaction(HER)and corrosion of the zinc anode significantly limit the development of Zn-ion ***,an inexpensive poly(3,4-ethylenedioxythiophene)(PEDOT)protective layer was constructed in situ on the Zn surface using electropolymerization to suppress dendrite growth and side reactions,thereby enhancing the reversibility of *** and theoretical calculations revealed that this hydrophilic protective layer promotes the desolvation process of hydrated Zn^(2+)and facilitates the transport of zinc ions,thus improving the thermodynamic and kinetic properties of Zn^(2+)deposition and inhibiting interfacial side ***,the optimized PEDOT@Zn symmetric battery exhibited a cycling stability exceeding 1250 h at 0.5 mA·cm^(-2)and 0.25 mAh·cm^(-2),with a significantly reduced overpotential(from 91.8 to 35 mV).With the assistance of the PEDOT protective layer,the PEDOT@Zn//Cu battery maintained approximately 99.5%Coulombic efficiency after 450 ***-situ scanning electron microscopy(SEM)and in situ optical microscopy characterizations further confirmed that the PEDOT protective layer can effectively suppress the growth of zinc ***,the Zn-ion capacitors assembled by the PEDOT@Zn and activated carbon also demonstrated outstanding cycling stability.

关键词： zinc-ion devices zinc anode poly(3 4-ethylenedioxythiophene)(PEDOT) dendrite desolvation process

Simulation Study on Translocation of Confined Chain Through Interacting Nanopore

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Chinese Journal of Chemical Physics 2012年第3期25卷 308-312,373页

作者： zi-bin zhou Hua-yu Li Yong-jun Xie Department of Information Science Anhui Economic Management Institute Hefei 230059 China School of Chemistry and Materials Science University of Science and Technology of China Hefei230026 China

The translocation of a confined polymer chain through an interacting nanopore has been studied using two-dimensional bond fluctuation model with Monte Carlo dynamics. For different pore-polymer interaction, the average escaping time （Tesc） of the polymer chain through the nanopore, increases roughly linearly with the chain length and the nanopore length~ respectively. However~ the large repulsive and attractive pore-polymer interaction adds the difficulty of the monomers of the chain entering and leaving the nanopore, respectively, leading to the nonmonotonical dependence of （Tesc） on the pore-polymer interaction. The detailed translocation dynamics of the chain through the interacting nanopore is inves- tigated too.

关键词： Monte Carlo simulation Polymer translocation Pore-polymer interaction Escaping time

A Novel Self-Supervised Learning Network for Binocular Disparity Estimation

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 209-229页

作者： Jiawei Tian Yu zhou Xiaobing Chen Salman A.AlQahtani hongrong Chen Bo Yang Siyu Lu Wenfeng Zheng Department of Computer Science and Engineering Major in Bio Artificial IntelligenceHanyang UniversityAnsan-si15577Republic of Korea School of Electrical and Computer Engineering Louisiana State UniversityBaton RougeLA 70803USA Department of Computer Engineering College of Computer and Information SciencesKing Saud UniversityRiyadh11574Saudi Arabia School of Automation University of Electronic Science and Technology of ChinaChengdu610054China

Two-dimensional endoscopic images are susceptible to interferences such as specular reflections and monotonous texture illumination,hindering accurate three-dimensional lesion reconstruction by surgical *** study proposes a novel end-to-end disparity estimation model to address these *** approach combines a Pseudo-Siamese neural network architecture with pyramid dilated convolutions,integrating multi-scale image information to enhance robustness against lighting *** study introduces a Pseudo-Siamese structure-based disparity regression model that simplifies left-right image comparison,improving accuracy and *** model was evaluated using a dataset of stereo endoscopic videos captured by the Da Vinci surgical robot,comprising simulated silicone heart sequences and real heart video *** results demonstrate significant improvement in the network’s resistance to lighting interference without substantially increasing ***,the model exhibited faster convergence during training,contributing to overall performance *** study advances endoscopic image processing accuracy and has potential implications for surgical robot applications in complex environments.

关键词： Parallax estimation parallax regression model self-supervised learning Pseudo-Siamese neural network pyramid dilated convolution binocular disparity estimation

Lipotoxic hepatocyte-derived exosomal miR-1297 promotes hepatic stellate cell activation through the PTEN signaling pathway in metabolic-associated fatty liver disease

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World Journal of Gastroenterology 2021年第14期27卷 1419-1434页

作者： Xin Luo Sheng-Zheng Luo zi-Xin Xu Cui zhou Zheng-hong Li Xiao-Yan zhou Ming-Yi Xu Department of Gastroenterology Shanghai General HospitalShanghai 200080China

BACKGROUND Exosomes play an important role in metabolic-associated fatty liver disease(MAFLD),but the mechanism by which exosomes participate in MAFLD still remain *** To figure out the function of lipotoxic exosomal miR-1297 in *** MicroRNA sequencing was used to detect differentially expressed miRNAs(DEmiR)in lipotoxic exosomes derived from primary *** tools were applied to analyze the target genes and pathways regulated by the *** real-time PCR(qPCR)was conducted for the verification of ***,western blot,immunofluorescence staining and ethynyl-20-deoxyuridine assay were used to evaluate the function of lipotoxic exosomal miR-1297 on hepatic stellate cells(LX2 cells).A luciferase reporter experiment was performed to confirm the relationship of miR-1297 and its target gene *** MicroRNA sequencing revealed that there were 61 exosomal DE-miRs(P2 from palmitic acid treated primary hepatocytes compared with the vehicle control ***-1297 was the most highly upregulated according to the microRNA *** tools showed a variety of target genes and pathways regulated by these DE-miRs were related to liver ***-1297 was overexpressed in exosomes derived from lipotoxic hepatocytes by *** promoting genes(α-SMA,PCNA)were altered in LX2 cells after miR-1297 overexpression or miR-1297-rich lipotoxic exosome incubation via qPCR and western blot *** staining and ethynyl-20-deoxyuridine staining demonstrated that the activation and proliferation of LX2 cells were also promoted after the above *** was found to be the target gene of miR-1297 and knocking down PTEN contributed to the activation and proliferation of LX2 cells via modulating the PI3K/AKT signaling *** miR-1297 was overexpressed in exosomes derived from lipotoxic *** lipotoxic hepatocyte-derived exosomal miR-1297 could promot

关键词： Metabolic-associated fatty liver disease miRNA-1297 Exosome Hepatic stellate cell PTEN Liver fibrosis

Nomogram for predicting the prognosis of tumor patients with sepsis after gastrointestinal surgery

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World Journal of Gastrointestinal Oncology 2022年第9期14卷 1771-1784页

作者： Ren-Xiong Chen zhou-Qiao Wu zi-Yu Li hong-Zhi Wang Jia-Fu Ji ICU Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Peking University Cancer Hospital&InstituteBeijing 100142China Department of Gastrointestinal Surgery Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Peking University Cancer Hospital&InstituteBeijing 100142China

BACKGROUND There were few studies on the prognosis of tumor patients with sepsis after gastrointestinal surgery and there was no relevant nomogram for predicting the prognosis of these *** To establish a nomogram for predicting the prognosis of tumor patients with sepsis after gastrointestinal surgery in the intensive care unit(ICU).METHODS A total of 303 septic patients after gastrointestinal tumor surgery admitted to the ICU at Peking University Cancer Hospital from January 1, 2013 to December 31, 2020 were analysed retrospectively. The model for predicting the prognosis of septic patients was established by the R software *** The most common infection site of sepsis after gastrointestinal surgery in the ICU was abdominal infection. The 90-d all-cause mortality rate was 10.2% in our study group. In multiple analyses, we found that there were statistically significant differences in tumor type, septic shock, the number of lymphocytes after ICU admission, serum creatinine and total operation times among tumor patients with sepsis after gastrointestinal surgery(P < 0.05). These five variables could be used to establish a nomogram for predicting the prognosis of these septic patients. The nomogram was verified, and the initial C-index was 0.861. After 1000 internal validations of the model, the C-index was 0.876, and the discrimination was good. The correction curve indicated that the actual value was in good agreement with the predicted *** The nomogram based on these five factors(tumor type, septic shock, number of lymphocytes, serum creatinine, and total operation times) could accurately predict the prognosis of tumor patients with sepsis after gastrointestinal surgery.

关键词： Tumor Surgery Sepsis Gastrointestinal Nomogram

Effect of sintering temperature on the microstructure and properties of foamed glass-ceramics prepared from high-titanium blast furnace slag and waste glass

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International Journal of Minerals,Metallurgy and Materials 2017年第8期24卷 931-936页

作者： Chang-hong Chen Ke-qin Feng Yu zhou hong-ling zhou School of Manufacturing Science and Engineering Sichuan University

Foamed glass-ceramics were prepared via a single-step sintering method using high-titanium blast furnace slag and waste glass as the main raw materials The influence of sintering temperature(900–1060℃) on the microstructure and properties of foamed glass-ceramics was studied. The results show that the crystal shape changed from grainy to rod-shaped and finally turned to multiple shapes as the sintering temperature was increased from 900 to 1060℃. With increasing sintering temperature, the average pore size of the foamed glass-ceramics increased and subsequently decreased. By contrast, the compressive strength and the bulk density decreased and subsequently increased. An excessively high temperature, however, induced the coalescence of pores and decreased the compressive strength. The optimal properties, including the highest compressive strength(16.64 MPa) among the investigated samples and a relatively low bulk density(0.83 g/cm^3), were attained in the case of the foamed glass-ceramics sintered at 1000℃.

关键词： foamed glass-ceramics sintering temperature blast furnace slag waste glass crystallization

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