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High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants

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Journal of Integrative Agriculture 2014年第5期13卷 1017-1023页

作者： SHEN Wan-xia Neil A Smith zhou Chang-yong WANG Ming-bo College of Plant Protection Southwest University CSIRO Plant Industry National Citrus Engineering Research Center Citrus Research Institute Chinese Academy of Agricultural Sciences

RNA silencing is a fundamental plant defence and gene control mechanism in plants that are directed by 20-24 nucleotide （nt） small interfering RNA （siRNA） and microRNA （miRNA）. Infection of plants with viral pathogens or transformation of plants with RNA interference （RNAi） constructs is usually associated with high levels of exogenous siRNAs, but it is unclear if these siRNAs interfere with endogenous small RNA pathways and hence affect plant development. Here we provide evidence that viral satellite RNA （satRNA） infection does not affect siRNA and miRNA biogenesis or plant growth despite the extremely high level of satRNA-derived siRNAs. We generated transgenic Nicotiana benthamiana plants that no longer develop the specific yellowing symptoms generally associated with infection by Cucumber mosaic virus （CMV） Y-satellite RNA （Y-Sat）. We then used these plants to show that CMV Y-Sat infection did not cause any visible phenotypic changes in comparison to uninfected plants, despite the presence of high-level Y-Sat siRNAs. Furthermore, we showed that the accumulation of hairpin RNA （hpRNA）-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not significantly affected by CMV Y-Sat infection. Taken together, our results suggest that the high levels of exogenous siRNAs associated with viral infection or RNAi-inducing transgenes do not saturate the endogenous RNA silencing machineries and have no significant impact on normal plant development.

关键词： Nicotiana benthamiana transformation satellite RNA RNA silencing small RNA

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Tempering Process to Improve Hardness Uniformity of Plastic Mould Steel

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Journal of Iron and Steel Research International 2012年第12期19卷 53-58页

作者： MIN yong-an zhou Quan LUO Yi LI Dan WU Xiao-chun School of Materials Science and Engineering Shanghai University Shanghai 200072 China

A new design of copper-bearing non-quenched plastic mold steel is presented and explained. Two kinds of microstrueture can be obtained from this new type copper-bearing steel via cooling with different cooling rates, bain- ite and a mixed microstructure consisting of ferrite and bainite. It is found that, after proper tempering process, the hardness will be increased. Moreover, the hardness difference between different microstructures will be reduced. For further investigation, the samples tempered at different temperatures were examined by XRD and 3PAP （three di- mensional atom probe） analysis. Results show that the improvement is contributed mainly by the precipitation of Cu phase and transformation of residual austenite.

关键词： plastic mould steel tempering process Cu precipitation residual austenite

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A Rapid Crack Detection Technique Based on Attention for Intelligent M&O of Cross-Sea Bridge

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China Ocean Engineering 2024年第5期38卷 866-876页

作者： zhou yong-chuan LI Guang-jun WEI Wei WANG Ya-meng JING Qiang Hong Kong-Zhuhai-Macao Bridge Authority Zhuhai 519015China National Observation and Research Station of Material Corrosion and Structural Safety of Hong Kong-Zhuhai-Macao Bridge in Guangdong Zhuhai 519000China Wuhan Maritime Communication Research Institute Wuhan 430079China

Rapid and accurate segmentation of structural cracks is essential for ensuring the quality and safety of engineering *** practice,however,this task faces the challenge of finding a balance between detection accuracy and *** alleviate this problem,a lightweight and efficient real-time crack segmentation framework was ***,in the network model system based on an encoding-decoding structure,the encoding network is equipped with packet convolution and attention mechanisms to capture features of different visual scales in layers,and in the decoding process,we also introduce a fusion module based on spatial attention to effectively aggregate these hierarchical *** are connected by pyramid pooling model(PPM)*** results show that the crack segmentation accuracy and real-time operation capability larger than 76%and 15 fps,respectively,are validated by three publicly available *** wide-ranging results highlight the potential of the model for the intelligent O&M for cross-sea bridge.

关键词： bridge defect detection crack detection lightweight design

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Complete genome sequence analysis of two Citrus tatter leaf virus(CTLV) isolates from China

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Journal of Integrative Agriculture 2015年第5期14卷 984-987页

作者： SONG Zhen LI Zhong-an LIU Ke-hong zhou Chang-yong Citrus Research Institute Southwest University/Chinese Academy of Agricultural Sciences

In order to understand molecular characterization of Citrus tatter leaf virus（CTLV） isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by *** complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6497 nucleotides in length and shared 79.9-91.0%and 78.8-98.0%nucleotide sequence identity,respectively,with other Apple stem grooving virus（ASGV） or CTLV strains available in ***,CTLV-MTH showed the highest nucleotide sequence identity（91%） with an apple isolate of ASGV,followed by 86.5%with ASGV-HH and 85.7%with ***,CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree,suggesting it has a closer relationship to ASGV than to ***,it can be concluded roughly that CTLV may be not a distinct strains of *** proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.

关键词： Citrus isolates Apple apple citrus sequenced length phylogenetic cloned orange

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Recent developments in nanofiltration membranes based on nanomaterials

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Chinese Journal of Chemical Engineering 2017年第11期25卷 1639-1652页

作者： Yanli Ji Weijie Qian Yawei Yu Quanfu An Lifen Liu yong zhou Congjie Gao Center for Membrane and Water Science&Technology Ocean CollegeZhejiang University of TechnologyHangzhou 310014China Beijing Key Laboratory for Green Catalysis and Separation College of Environmental and Energy EngineeringBeijing University of TechnologyBeijing 100124China

Nanofiltration membranes are the core elements for nanofiltration process. The chemical structures and physical properties of nanofiltration membranes determine water permeability, solute selectivity, mechanical/thermal stability, and antifouling properties, which greatly influence the separation efficiency and operation cost in nanofiltration applications. In recent years, a great progress has been made in the development of high performance nanofiltration membranes based on nanomaterials. Considering the increasing interest in this field, this paper reviews the recent studies on the nanofiltration membranes comprising various nanomaterials, including the metal and metal oxide nanoparticles, carbon-based nanomaterials, metal–organic frameworks(MOFs), water channel proteins, and organic micro/nanoparticles. Finally, a perspective is given on the further exploitation of advanced nanomaterials and novel strategy for fabricating nano-based nanofiltration membranes. Moreover,the development of precision instruments and simulation techniques is necessary for the characterization of membrane microstructure and investigation of the separation and antifouling mechanism of nanofiltration membranes prepared with nanomaterials.

关键词： Nanofiltration Separation Membranes Nanocomposite Nanomaterials

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BLOCK ITERATIVE METHODS FOR LINEAR ALGEBRAIC EQUATION AND DOMAIN DECOMPOSITION METHOD FOR INCOMPRESSIBLE VISCOUS FLOW

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Journal of Hydrodynamics 2002年第4期14卷 73-80页

作者： Ren, An-Lu Zou, Jian-Feng zhou, yong-Xia Li, Guang-Wang Dept. of Mech. Zhejiang Univ. Hangzhou 310027 China

It was proved numerically that the Domain Decomposition Method CDDM) with one layer overlapping grids is identical to the block iterative method of linear algebra equations. The results obtained by using DDM could be in reasonable agreement with the results of full-domain simulation. With the three dimensional solver developed by the authors, the flow field in a pipe was simulated by using the full-domain DDM with one layer overlapping grids and with patched grids respectively. Both of the two cases led to the convergent solution. Further research shows the superiority of the DDM with one layer overlapping grids to the DDM with patched grids. A comparison between the numerical results obtained by the authors and the experimental results given by Enayet[3] shows that the numerical results are reasonable.

关键词： Computational fluid dynamics Computer simulation Iterative methods Linear equations Viscous flow

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A carbon monoxide sensor based on single-walled carbon nanotubes doped with copper chloride

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Science China(Technological Sciences) 2013年第10期56卷 2576-2580页

作者： ZHANG GuangDi TAI HuiLing XIE GuangZhong JIANG YaDong zhou yong State Key Laboratory of Electronic Thin Films and Integrated Devices School of Optoelectronic Information University of Electronic Science and Technology of China (UESTC)

A carbon monoxide gas sensor based on single-walled carbon nanotube （SWCNT） has been developed for detection of carbon monoxide （CO） at room temperature. Copper chloride （CuC1） was mixed with SWNT by mechanical blending. The thin film was deposited on interdigitated electrodes by using airbrush technology. This paper described the fabrication of the sensor for detecting carbon monoxide with concentrations from 20 ppm to 100 ppm. The performance of CO gas sensor was measured by using relevant apparatus to obtain the continuous sensor electric resistance change on exposure to CO and air atmosphere at room temperature, respectively. The results exhibited that the senor presented a larger sensitivity and a good recoverability. The experimental results suggested the potential use of CuC1 doped SWCNT for CO detecting.

关键词： carbon monoxide gas sensor single-walled carbon nanotubes copper chloride room temperature

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A novel nonparametric mixture model for the detection pattern of COVID-19 on Diamond Princess cruise

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Statistical Theory and Related Fields 2023年第1期7卷 85-96页

作者： Huijuan Ma Jing Qin Fang Chen yong zhou KLATASDS-MOE School of Statistics and Academy of Statistics and Interdisciplinary SciencesEast China Normal UniversityShanghaiPeople’s Republic of China National Institute of Allergy and Infectious Diseases National Institutes of HealthBethesdaMDUSA School of Statistics and Management Shanghai University of Finance and EconomicsShanghaiPeople’s Republic of China

The outbreak of COVID-19 on the Diamond Princess cruise ship has attracted much *** by the PCR testing data on the Diamond Princess,we propose a novel cure mixture nonparametric model to investigate the detection *** combines a logistic regression for the probability of susceptible subjects with a nonparametric distribution for the detection of infected *** likelihood estimators are *** resulting estimators are shown to be consistent and asymptotically *** studies demonstrate that the proposed approach is appropriate for practical ***,we apply the proposed method to PCR testing data on the Diamond Princess to show its practical utility.

关键词： Cure model logistic regression maximum likelihood estimator mixture

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Suitability of SDN and MEC to facilitate digital twin communication over LTE-A

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Digital Communications and Networks 2024年第2期10卷 347-354页

作者： Hikmat Adhami Mohammad Alja’afreh Mohamed Hoda Jiaqi Zhao yong zhou Abdulmotaleb El Saddik Mohamed bin Zayed University of Artificial Intelligence Masdar CityAbu DhabiUnited Arab Emirates School of Computer Science and Technology China University of Mining and TechnologyXuzhou221116China School of Electrical Engineering and Computer Science University of OttawaOttawaONK1N 6N5Canada

Haptic is the modality that complements traditional multimedia,i.e.,audiovisual,to evolve the next wave of innovation at which the Internet data stream can be exchanged to enable remote skills and control *** will require ultra-low latency and ultra-high reliability to evolve the mobile experience into the era of Digital Twin and Tactile *** the 5th generation of mobile networks is not yet widely deployed,Long-Term Evolution(LTE-A)latency remains much higher than the 1 ms requirement for the Tactile Internet and therefore the Digital *** work investigates an interesting solution based on the incorporation of Software-defined networking(SDN)and Multi-access Mobile Edge Computing(MEC)technologies in an LTE-A network,to deliver future multimedia applications over the Tactile Internet while overcoming the QoS *** network scenarios were designed and simulated using Riverbed modeler and the performance was evaluated using several time-related Key Performance Indicators(KPIs)such as throughput,End-2-End(E2E)delay,and *** best scenario possible is clearly the one integrating MEC and SDN approaches,where the overall delay,jitter,and throughput for haptics-attained 2 ms,0.01 ms,and 1000 packets per *** results obtained give clear evidence that the integration of,both SDN and MEC,in LTE-A indicates performance improvement,and fulfills the standard requirements in terms of the above KPIs,for realizing a Digital Twin/Tactile Internet-based system.

关键词： LTE-A 5G Digital twin Tactile Internet KPIs SDN MEC NFV Haptic

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Identification and Characterization of Tonoplast Sugar Transporter (TST) Gene Family in Cucumber

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Horticultural Plant Journal 2020年第3期6卷 145-157页

作者： Weifeng Huang Bin Hu Jialin Liu yong zhou Shiqiang Liu National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research Huazhong Agricultural UniversityWuhan 430070China Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of EducationJiangxi Agricultural UniversityNanchang 330045China College of Bioscience and Bioengineering Jiangxi Agricultural UniversityNanchang 330045China

Tonoplast sugar transporters(TSTs)play essential roles in regulating plant growth,development,and response to various biotic and abiotic *** this study,a total of three TST genes were identified by a genome-wide analysis in *** analysis showed that TST proteins from cucumber and other plant species can be classified into five groups,and nearly all TST members in the same groups displayed similarmotif distributions,transmembrane(TM)domains,and gene *** of the three CsTST genes possess a number of development-,stress-,and hormone-related cis-elements in the promoter ***,qRT-PCR assays revealed that the CsTST1 was expressed in fruits,flowers,leaves,and other tissues,and its expression varied significantly under various abiotic stresses such as cold,salt,drought(PEG),and abscisic acid(ABA).Finally,functional analysis of CsTST1 in yeast revealed that it was able to complement the deficiency in galactose,mannose and sucrose *** results revealed that CsTST1 can act as a functional sugar transporter to play important roles in cucumber growth and response to abiotic stress probably through affecting carbohydrate distribution.

关键词： Cucumber Cucumis sativus L. TST Phylogenetic analysis Gene expression Abiotic stress Sugar transport

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