Identification and Characterization of Tonoplast Sugar Transporter (TST) Gene Family in Cucumber

作     者：Weifeng Huang Bin Hu Jialin Liu Yong Zhou Shiqiang Liu Weifeng Huang;Bin Hu;Jialin Liu;Yong Zhou;Shiqiang Liu

作者机构：National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene ResearchHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Crop PhysiologyEcology and Genetic BreedingMinistry of EducationJiangxi Agricultural UniversityNanchang 330045China College of Bioscience and BioengineeringJiangxi Agricultural UniversityNanchang 330045China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2020年第6卷第3期

页      面：145-157页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：the Key Project of Science Foundation of Jiangxi Province(Grant Nos.20171ACB21025 and 20181ACB20012) the National Natural Science Foundation of China(Grant No.31760074) 

主　　题：Cucumber Cucumis sativus L. TST Phylogenetic analysis Gene expression Abiotic stress Sugar transport 

摘      要：Tonoplast sugar transporters(TSTs)play essential roles in regulating plant growth,development,and response to various biotic and abiotic *** this study,a total of three TST genes were identified by a genome-wide analysis in *** analysis showed that TST proteins from cucumber and other plant species can be classified into five groups,and nearly all TST members in the same groups displayed similarmotif distributions,transmembrane(TM)domains,and gene *** of the three CsTST genes possess a number of development-,stress-,and hormone-related cis-elements in the promoter ***,qRT-PCR assays revealed that the CsTST1 was expressed in fruits,flowers,leaves,and other tissues,and its expression varied significantly under various abiotic stresses such as cold,salt,drought(PEG),and abscisic acid(ABA).Finally,functional analysis of CsTST1 in yeast revealed that it was able to complement the deficiency in galactose,mannose and sucrose *** results revealed that CsTST1 can act as a functional sugar transporter to play important roles in cucumber growth and response to abiotic stress probably through affecting carbohydrate distribution.