检索结果-南通市图书馆

Legionella dumoffii Tex-KL Mutated in an Operon Homologous to traC-traD is Defective in Epithelial Cell Invasion

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2016年第6期29卷 424-434页

作者： QIN Tian Iida Ken-ichiro REN Hong Yu zhou hai jian Shin-ichi Yoshida State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention beijing 102206 China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310003 Zhejiang China Departments of Bacteriology Faculty of Medical Sciences Kyushu University Higashi-ku Fukuoka 812-8582 Japan

Objective To understand the mechanism of invasion by Legionella dumoffii. Methods The L. dumoffii strain Tex-KL was mutated using the Tn903 derivative, Tn903 d IIlac Z. After screening 799 transposon insertion mutants, we isolated one defective mutant. We then constructed the gene-disrupted mutant, KL16, and studied its invasion of and intracellular growth in He La and A549 cells, and in A/J mice survival experiments. The structure of traC-traD operon was analyzed by RT-PCR. Results The transposon insertion was in a gene homologous to Salmonella typhi tra C, which is required for the assembly of F pilin into the mature F pilus structure and for conjugal DNA transmission. Results from RT-PCR suggested that the traC-traD region formed an operon. We found that when the tra C gene was disrupted, invasion and intracellular growth of L. dumoffii Tex-KL were impaired in human epithelial cells. When mice were infected by intranasal inoculation with a tra C deficient mutant, their survival significantly increased when compared to mice infected with the wild-type strain. Conclusion Our results indicated that the traC-traD operon is required for the invasion and intracellular growth abilities of L. dumoffii Tex-KL in epithelial cells.

关键词： L. dumoffii Tex-KL traC-traD operon Invasion ability Epithelial cells

Optimization of Pulse-Field Gel Electrophoresis for Borrelia burgdorferi Subtyping

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第7期26卷 584-591页

作者： GENG Zhen HOU Xue Xia HAO Qin zhou hai jian WANG Feng WAN Kang Lin State Key Laboratory for Communicable Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Department of Infectious Disease The First Hospital Jilin University

Objective To optimize the performance of Pulsed-Field Gel Electrophoresis （PFGE） for the comparison of inter-laboratory results and information exchange of Borrelia burgdorferi subtypingo Methods A panel of 34 strains of B. burgdorferi were used to optimize PFGE for subtyping. In order to optimize the electrophoretic parameters （EPs）, all 34 strains of B. burgdorferi were analyzed using four EPs, yielding different Simpson diversity index （D） values and the epidemiological concordance was also evaluated. Results The EP of a switch time of l s to 25 s for13 h and l s to10 s for 6 h produced the highest D value and was declared to be optimal for Mlul and 5mal PFGE of B. burgdorferi. Mlul and Smal were selected as the first and second restriction enzymes for PFGE subtyping of B. burgdorferi according to discrimination and consistency with epidemiological data. Conclusion PFGE can be used as a valuable test for routine genospecies identification of B burgdorferi.

关键词： Molecular subtyping Pulse-Field Gel Electrophoresis Borrelia burgdor^eri

Simultaneous Detection of 13 Key Bacterial Respiratory Pathogens by Combination of Multiplex PCR and Capillary Electrophoresis

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2017年第8期30卷 549-561页

作者： jianG Lu Xi REN Hong Yu zhou hai jian ZHAO Si Hong HOU Bo Yan YAN jian Ping QIN Tian CHEN Yu Department of Respiratory Medicine Shengjing Hospital of China Medical University National Institute for Communicable Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention Department of Respiratory Medicine Zhejiang Provincial People's Hospital

Objective Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction （PCR） and capillary electrophoresis （MPCE） assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catorrholis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp., Bordetella pertussis, Mycobacterium tuberculosis complex, Corynebactefium diphthefiae, and Streptococcus pyogenes. Methods Three multiplex PCR reactions were built, and the products were analyzed by capillary electrophoresis using the high-throughput DNA analyzer. The specificity of the MPCE assay was examined and the detection limit was evaluated using DNA samples from each bacterial strain and the simulative samples of each strain. This assay was further evaluated using 152 clinical specimens and compared with real-time PCR reactions. For this assay, three nested-multiplex-PCRs were used to detect these clinical specimens. Results The detection limits of the MPCE assay for the 13 pathogens were very low and ranged from 10-7 to 10-2 ng/μL. Furthermore, analysis of the 252 clinical specimens yielded a specificity ranging from 96.5%-100.0%, and a sensitivity of 100.0% for the 13 pathogens. Conclusion This study revealed that the MPCE with high specificity and sensitivity. This assay survey of respiratory pathogens. assay is a rapid, reliable, and high-throughput method has great potential in the molecular epidemiological.

关键词： Respiratory pathogens Lower respiratory tract infections Multiplex PCR Capillary electrophoresis

An Investigation on the Molecular Characteristics and Intracellular Growth Ability among Environmental and Clinical Isolates of Legionella pneumophila in Sichuan Province, China

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2019年第7期32卷 520-530页

作者： ZENG Lin Zi LIAO Hong Yu LUO Long Ze HE Shu Sen QIN Tian zhou hai jian LI Hong Xia CHEN Da Li CHEN jian Ping Department of Parasitology West China School of Basic Medical Sciences & Forensic Medicine Sichuan University Chengdu 610041 Sichuan China Sichuan Center for Disease Control and Prevention Chengdu 610041 Sichuan China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Chengdu first people's Hospital Chengdu 610041 Sichuan China

Objective To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila(L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China. Methods Seventy-nine isolates of L. pneumophila were collected from environmental and clinical sources, including cooling towers, hot springs, bath water, fountains, and patients, and identified with 16 S rRNA gene analysis and serum agglutination assay. The isolates were then typed by Sequence-Based Typing(SBT), and Genotyping of forty-two LP1 strains were analyzed by means of multiple-locus VNTR analysis with 8 loci(MLVA-8). All strains were further analyzed for two virulence genes: Legionella vir homologue(lvh) and repeats in structural toxin(rtx A). The intracellular growth ability of 33 selected isolates was determined by examining their interaction with J774 cells. Results All isolates were identified to L. pneumophila including 11 serogroups, among which the main serogroup were LP1, accounting for 54.43%. Thirty-three different sequence types(STs) from five main clonal groups and five singletons were identified, along with 8 different MLVA patterns. Both the lvh and rtx A loci were found in all 79 strains. Thirty isolates showed high intracellular growth ability in J774 cells. Conclusion L. pneumophila is a potential threat to public health, and effective control and prevention strategies are urgently needed.

关键词： Molecular characteristics Intracellular growth ability Environments Clinical L.pneumophila Sichuan

Molecular Characteristics of Neisseria meningitidis Isolated during an Outbreak in a Jail: Association with the Spread and Distribution of ST-4821 Complex Serogroup C Clone in China

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第5期26卷 331-337页

作者： ZHANG Ji zhou hai jian XU Li HU Guang Chun ZHANG Xue Hua XU Sheng Ping LIU Zun Yu SHAO Zhu Jun Jinan Municipal Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention

Objective To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyperinvasive ST-4821 serogroup C clone associated with this outbreak. Methods The cases were described based on the clinical symptoms and laboratory results. Pharyngeal swabs were cultured for N. meningitidis from men in the jail. Meningococcal isolates were identified by serogrouping, pulsed-field gel electrophoresis （PFGE） and multilocus sequence typing （MLST）, respectively. Four hundred and sixteen serogroup C N. meningitidis strains were collected from 27 provinces between 2003 and 2010 for a nationwide survey and analyzed by PFGE and MLST. Results Three persons in a jail system were infected with invasive N. meningitidis serogroup C. All isolates tested had matching PFGE patterns and belonged to the multilocus sequence type （ST） 4821 clonal complex. All 47 N. meningitidis strains were identified from the pharyngeal swabs of 166 peoples in the jail, and 26 of them belonged to ST-4821 serogroup C clone, and 90.14% （375/416） serogroup C strains identified in the nationwide survey belonged to the ST-4821 complex. The ST-4821 serogroup C clone was spread nationwide, distributed in 24 provinces, especially in eastern provinces between 2003 and 2010. Conclusion Endemic transmission and carriage rate of ST-4821 serogroup C clone are high in this jail system. The ST-4821 serogroup C clone is spreading in China and nationwide distributed despite the existence of some effective vaccines.

关键词： Pulsed-field gel electrophoresis Multilocus sequence typing Neisseria meningitidis ST-4821 Serogroup C

Comparative Study of the Genetic Diversity, Antimicrobial Resistance, and Pathogenicity of Aeromonas Isolates from Clinical Patients and Healthy Individuals

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2021年第6期34卷 454-464页

作者： MENG Shuang DU Xiao Li WANG Yong Lu QU Feng Tian XIE Gui Lin zhou hai jian HU Jin Rui QIN Zheng WANG Yue KAN Biao CUI Zhi Gang State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Ma'anshan Center for Disease Control and Prevention Ma'anshan 243000AnhuiChina College of Life Science Northeast Agricultural UniversityHa'erbin 150000HeilongjiangChina School of Public Health Jiamusi UniversityJiamusi 154000HeilongjiangChina

Objective This study was performed to compare the genetic diversity,virulence,and antimicrobial resistance of Aeromonas strains isolated from patients and healthy *** A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma’anshan City,Anhui *** taxonomy was investigated using concatenated gyrB-cpn60 sequences,and their resistance to 12 antibiotics was *** pathogenicity of these strains was examined through beta-hemolysis,protease activity,and virulence gene *** The 57 Aeromonas strains were divided into 55 sequence *** these types,21 were novel,suggesting that their genetic diversity was *** Aeromonas isolates could be divided into 7 species,and the positive rates of beta-hemolysis and protease activity were 49.1%and 73.7%,*** detection rate of clinical patients in terms of beta-hemolysis and protease activity was higher than that of healthy *** the four most common Aeromonas strains,*** had the highest detection rate of virulence *** multidrug resistance rate of the clinical isolates was much higher than that of the strains isolated from healthy *** The taxonomy,virulence properties,and antibiotic resistance of Aeromonas isolates from patients differ from those of the isolates from healthy individuals.

关键词： Aeromonas Genetic diversity Multidrug resistance Virulence gene Antimicrobial resistance gene

Genetic Diversity,Antibiotic Resistance,and Pathogenicity of Aeromonas Species from Food Products in Shanghai,China

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第9期35卷 842-853页

作者： QU Feng Tian WANG Wen Qing LIU Qian zhou hai jian HU Jin Rui DU Xiao Li WANG Yue XUE Jia Qi CUI Zhi Gang XIE Gui Lin MENG Shuang College of Life Science Northeast Agricultural UniversityHa’erbin 150000HeilongjiangChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Shanghai Pudong New Area Center for Disease Control and Prevention Shanghai 200136China Laboratory Medicine Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Clinic Medical College North China University of Science and TechnologyTangshan 063000HebeiChina

Objective Aeromonas has recently been recognized as an emerging human *** diarrhea is a phenomenon occurring *** study was designed to determine the prevalence,genetic diversity,antibiotic resistance,and pathogenicity of Aeromonas strains isolated from food products in *** Aeromonas isolates(n=79)collected from food samples were analyzed using concatenated gyrB-cpn60 *** antibiotic resistance of these isolates was determined using antimicrobial susceptibility *** was assessed usingβ-hemolytic,extracellular protease,virulence gene detection,*** liquid toxicity(LT),and cytotoxicity *** Eight different species were identified among the 79 *** most prevalent Aeromonas species were ***[62(78.5%)],***[6(7.6%)],***[3(3.8%)],and ***[3(3.8%)].The Aeromonas isolates were divided into 73 sequence types(STs),of which 65 were *** isolates were hemolytic(45.6%)and protease-positive(81.0%).The most prevalent virulence genes were act(73.4%),fla(69.6%),aexT(36.7%),and ascV(30.4%).The results of *** LT and cytotoxicity assays revealed that *** and *** were more virulent than ***,***,and *** resistance genes[tetE,blaTEM,tetA,qnrS,aac(6)-Ib,mcr-1,and mcr-3]were detected in the *** multidrug-resistance rate of the Aeromonas isolates was 11.4%,and 93.7%of the Aeromonas isolates were resistant to *** The taxonomy,antibiotic resistance,and pathogenicity of different Aeromonas species *** Aeromonas isolates *** and *** were highly pathogenic,indicating that food-derived Aeromonas isolates are potential risks for public health and food *** monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China.

关键词： Aeromonas Genetic diversity Antibiotic resistance Virulence gene Cytotoxicity assay Multidrug-resistance

在线全文

学校读者我要写书评

暂无评论

WELL建筑标准在住宅中的设计理念分析

绿色建筑 2019年第3期11卷 12-14页

作者：周海舰誉德生态技术咨询(上海)有限公司

居住建筑与人们的健康状况密切相关,健康建筑亦是近年来备受关注的热点问题。以住宅为例,详细分析了WELLv2建筑标准的10大概念在住宅建筑中的设计实施情况,阐述了这些条款措施对人体身心健康的积极影响。这些研究成果可为住宅建筑取得W... 详细信息

居住建筑与人们的健康状况密切相关,健康建筑亦是近年来备受关注的热点问题。以住宅为例,详细分析了WELLv2建筑标准的10大概念在住宅建筑中的设计实施情况,阐述了这些条款措施对人体身心健康的积极影响。这些研究成果可为住宅建筑取得WELL认证的设计和实践提供一定参考。

关键词： WELL建筑标准住宅建筑人体健康设计分析

Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan Huang Yi-De Zhang Jie Chen hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li hai-Wei Pu Xin-Yian Guo jian-Gang Shen Su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin