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Effect of Loss-of-function of the Herpes Simplex Virus-1 microRNA H6-5p on Virus Replication

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Virologica Sinica 2019年第4期34卷 386-396页

作者： Rongquan Huang Xusha zhou Shuqi Ren Xianjie Liu Zhiyuan Han Grace guoying zhou School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhou 511436China Shenzhen International Institute for Biomedical Research Shenzhen 518116China

To date, 29 distinct microRNAs(miRNAs) have been reported to be expressed during herpes simplex virus *** analysis of mature herpes simplex virus-1(HSV-1) miRNAs revealed five sets of miRNAs that are complementary to each other: miR-H6-5p/H1-3p, miR-H6-3p/H1-5p, H2-5p/H14-3p, miR-H2-3p/H14-5p, and miR-H7/***, the roles of individual miRNAs and consequences of this complementarity remain unclear. Here, we focus on two of these complementary miRNAs, miR-H6-5p and miR-H1-3p, using loss-of-function experiments in vitro and in a mouse model of infection using an miRNA sponge approach, including tandem multiplex artificial miRNA-binding sequences that do not match perfectly to the target miRNA inserted downstream of a green fluorescent protein reporter gene. Infection with recombinant virus expressing the miR-H6-5p sponge reduced viral protein levels and virus *** accumulation of viral proteins was also observed at early stages of infection in the presence of both an miR-H6-5p inhibitor and plasmid-expressed miR-H1-3p. Moreover, establishment of latency and reactivation did not differ between the recombinant virus expressing the miR-H6-5p sponge and wild-type HSV-1. Taken together, these data suggest that miR-H6-5p has an as-yet-unidentified role in the early stages of viral infection, and its complement miR-H1-3p suppresses this role in later stages of infection. This report extends understanding of the roles of miRNAs in infection by herpes simplex viruses, supporting a model of infection in which the production of virus and its virulent effects are tightly controlled to maximize persistence in the host and population.

关键词： miR-H6-5p miR-H1-3p Sponge Replication Herpes simplex virus-1(HSV-1)

Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

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Chinese Science Bulletin 2001年第9期46卷 766-770,794页

作者： LIU Zhiyi XIANG Jianhai zhou guoying GONG Zuxun Experimental Marine Biology Laboratory Institute of OceanologyChinese Academy of SciencesQingdao 266071China Shanghai Institute of Biochemistry Chinese Academy of SciencesShanghai 200031China

Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.

关键词： gene gun GFP transgenic shrimps.

Termination of Transcription of LAT Increases the Amounts of ICP0 mRNA but Does Not Alter the Course of HSV-1 Infection in Latently Infected Murine Ganglia

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Virologica Sinica 2021年第2期36卷 264-272页

作者： Haifang Jiang Jiaming Wu Xianjie Liu Ruitao Lu Manling zhou Meiling Chen Yonghong Liu Grace guoying zhou Wenmin Fu School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhou 511436China Shenzhen International Institute for Biomedical Research Shenzhen 518116China

On entering sensory ganglia,herpes simplex viruses 1(HSV-1)establishes a latent infection with the synthesis of a latency associated transcript(LAT)or initiates productive infection with expression of a set of immediate early viral *** precise mechanisms how expression of a genes is suppressed during the latency are *** mechanism that has been proposed is illustrated in the case of ICP0,a key immediate early viral regulatory ***,the 2 kb LAT intron is complementary to the 30 terminal portion of ICP0 m *** test the hypothesis that accumulation of LAT negatively affects the accumulation of ICP0 m RNA,we inserted a DNA fragment encoding two poly(A)sequences into LAT to early terminate LAT transcript without interrupting the complementary sequence of ICP0 transcript(named as SR1603).Comparisons of the parent(SR1601)and mutant(SR1603)HSV-1 viruses showed the following:Neurons harboring latent SR1603 virus accumulated equivalent amounts of viral DNA but higher amounts of ICP0 m RNA and lower amounts of LAT,when compared to neurons harboring the SR1601 *** notable difference between the two viruses is that viral RNA accumulation in explanted ganglia harboring SR1603 virus initiated significantly sooner than that in neurons harboring SR1601 virus,suggesting that ICP0 may act as an activator of viral gene expression in permissive ***,these data suggest that increased ICP0 m RNA by suppressed LAT did not affect the establishment of latency in latently infected murine ganglia.

关键词： Herpes simplex viruses 1(HSV-1) Latency associated transcript(LAT) ICP0 Latency

Above-and belowground trait linkages of dominant species shape responses of alpine steppe composition to precipitation changes in the Tibetan Plateau

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Journal of Plant Ecology 2021年第4期14卷 569-579页

作者： Zhi Zheng Yue Zhang Shihu Zhang Qun Ma Dajie Gong guoying zhou College of Life Science Northwest Normal UniversityLanzhou 730070China Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of ScienceXining 810008China

Aims Human activities and global changes have led to alterations in global and regional precipitation *** extensive studies on the effects of changes in precipitation regimes on plant community composition across different types of grassland worldwide,few studies have specifically focused on the effects of precipitation changes on high-altitude alpine steppe at community and plant species levels in the Tibetan *** We investigated the effects of growing-season precipitation changes(reduced precipitation by 50%,ambient precipitation,enhanced precipitation by 50%)for 6 years on plant community composition in an alpine steppe of the Tibetan Plateau by linking above-to belowground traits of dominant *** Findings We found that reduced precipitation shifted community composition from dominance by bunchgrass(primarily Stipa purpurea)to dominance by rhizomatous grass(primarily Leymus secalinus).Roots and leaf traits of *** and *** differed in their responses to reduced *** precipitation enhanced root vertical length and carbon(C)allocation to deep soil layers,and decreased the leaf width in ***,but it did not change the traits in ***,reduced precipitation significantly enhanced rhizome biomass,length,diameter and adventitious root at the rhizome nodes in *** changes in traits may render rhizomatous grass greater competitive during drought ***,our findings highlight important roles of above-and belowground traits of dominant species in plant community composition of alpine steppe under precipitation change.

关键词： precipitation change functional group dominant species Stipa purpurea Leymus secalinus soil moisture plant traits alpine steppe Tibetan Plateau

Permafrost carbon cycle and its dynamics on the Tibetan Plateau

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Science China(Life Sciences) 2024年第9期67卷 1833-1848页

作者： Leiyi Chen Guibiao Yang Yuxuan Bai Jinfeng Chang Shuqi Qin Futing Liu Mei He Yutong Song Fan Zhang Josep Penuelas Biao Zhu guoying zhou Yuanhe Yang State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310058China Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration Ecology and Nature Conservation InstituteChinese Academy of ForestryBeijing 100091China University of Chinese Academy of Sciences Beijing 100049China Consejo Superior de Investigaciones Científicas(CSIC) Global Ecology Unit CREAF-CSIC-UAB(Universitat Autònoma de Barcelona)Barcelona 08193Spain Centre for Ecological Research and Forestry(CREAF) Barcelona 08193Spain Institute of Ecology College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking UniversityBeijing 100871China Northwest Institute of Plateau Biology Chinese Academy of SciencesXining 810008China Key Laboratory of Tibetan Medicine Research Chinese Academy of SciencesXining 810008China

Our knowledge on permafrost carbon(C)cycle is crucial for understanding its feedback to climate warming and developing nature-based solutions for mitigating climate *** understand the characteristics of permafrost C cycle on the Tibetan Plateau,the largest alpine permafrost region around the world,we summarized recent advances including the stocks and fluxes of permafrost C and their responses to thawing,and depicted permafrost C dynamics within this *** find that this alpine permafrost region stores approximately 14.1 Pg(1 Pg=1015g)of soil organic C(SOC)in the top 3 *** substantial gaseous emissions and lateral C transport occur across this permafrost ***,the mobilization of frozen C is expedited by permafrost thaw,especially by the formation of thermokarst landscapes,which could release significant amounts of C into the atmosphere and surrounding water *** alpine permafrost region nevertheless remains an important C sink,and its capacity to sequester C will continue to increase by *** future perspectives,we would suggest developing long-term in situ observation networks of C stocks and fluxes with improved temporal and spatial coverage,and exploring the mechanisms underlying the response of ecosystem C cycle to permafrost *** addition,it is essential to improve the projection of permafrost C dynamics through in-depth model-data fusion on the Tibetan Plateau.

关键词： permafrost carbon climate change carbon release carbon sink permafrost thaw thermokarst formation

C:N:P stoichiometry of Ericaceae species in shrubland biomes across Southern China:influences of climate,soil and species identity

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Journal of Plant Ecology 2019年第2期12卷 346-357页

作者： Qiang Zhang Qing Liu Huajun Yin Chunzhang Zhao Lin Zhang guoying zhou Chunying Yin Zhijun Lu Gaoming Xiong Yuelin Li Jiaxiang Li Wenting Xu Zhiyao Tang Zongqiang Xie State Key Laboratory of Vegetation and Environment Change Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Key laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China Key Laboratory of Alpine Ecology and Biodiversity of Chinese Academy of Sciences Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China Northwest Institute of Plateau Biology Chinese Academy of SciencesXining 810008 Key Laboratory of Aquatic Botany and Watershed Ecology Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China College of Forest Central South University of Forestry&TechnologyChangsha 410004China Department of Ecology College of Urban and Environmental Sciences and Key Laboratory for Earth Surface ProcessesPeking UniversityBeijing 100871China

Aims Carbon(C),nitrogen(N)and phosphorus(P)stoichiometry strongly affect functions and nutrient cycling within ***,the related researches in shrubs were very *** this study,we aimed to inves-tigate leaf stoichiometry and its driving factors in shrubs,and whether stoichiometry significantly differs among closely related *** We analyzed leaf C,N and P concentrations and their ratios in 32 species of Ericaceae from 161 sites across southern *** examined the relationships of leaf stoichiometry with environmen-tal variables using linear regressions,and quantified the interactive and independent effects of climate,soil and species on foliar stoi-chiometry using general linear models(GLM).Important Findings The foliar C,N and P contents of Ericaceae were 484.66,14.44 and 1.06 mg g−1,*** C,N and P concentrations and their ratios in Ericaceae were significantly related with latitude and altitude,except the N:P insignificantly correlated with ***(mean annual temperature and precipitation)and soil properties(soil C,N and P and bulk density)were significantly influenced element *** GLM analysis showed that soil exerted a greater direct effect on leaf stoichiometry than climate did,and climate affected leaf traits mainly via indirect ***,soil properties had stronger influ-ences on leaf P than on leaf C and *** all independent factors examined,we found species accounted for the largest proportion of the variation in foliar *** results suggest that species can largely influence foliar stoichiometry,even at a lower taxonomic level.

关键词： biogeographic pattern phylogenetic effect closely related species,Ericoid Mycorrhiza shrub

Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation

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Protein & Cell 2020年第5期11卷 366-373页

作者： Nan Wang Wenyuan Chen Ling Zhu Dongjie Zhu Rui Feng Jialing Wang Bin Zhu Xinzheng Zhang Xiaoqing Chen Xianjie Liu Runbin Yan Dongyao Ni Grace guoying zhou Hongrong Liu Zihe Rao Xiangxi Wang CAS Key Laboratory of Infection and Immunity National Laboratory of MacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Key Laboratory for Matter Microstructure and Function of Hunan Province Key Laboratory of Low-dimensional Quantum Structures and Quantum ControlSchool of Physics and ElectronicsHunan Normal UniversityChangsha 410081China Laboratory of Structural Biology Tsinghua UniversityBeijing 100084China State Key Laboratory of Medicinal Chemical Biology Nankai UniversityTianjin 300353China ImmVira Co. LtdSilver Star Hi-tech Industrial ParkLonghua DistrictShenzhen 518116China

Dear Editor,Herpesviridae is a large family of double-stranded DNA(dsDNA)viruses that cause a variety of human diseases ranging from cold sores and chicken pox to congenital defects,blindness and cancer(Chayavichitsilp et al.,2009;Wang et al.,2018).In the past 70 years,substantial advances in our knowledge of the molecular biology of herpesviruses have led to insights into disease pathogenesis and ***,the mechanism for capsid assembly that requires the ordered packing of about 4,000 protein subunits into the hexons,pentons and triplexes remains *** is still a puzzle how initially identical subunits adopt both hexameric and pentameric conformations in the capsid and select the correct locations needed to form closed shells of the proper *** and genetic studies have shown that the portal is involved in initiation of capsid assembly(Newcomb et al.,2005)and functions akin to a DNA-sensor coupling genome-packaging achieved by a genome-packaging machinery-“terminase complex”(Chen et al.,2020;Yunxiang Yang,2020)with icosahedral capsid maturation(Lokareddy et al.,2017).Structural investigations of the herpesvirus portal have proven challenging due to the small size of this dodecamer,which accounts for less than 1%of the total mass of the capsid protein layer and the technical difficulties involved in resolving non-icosahedral components of such large icosahedral viruses(diameter is∼1,250Å).Efforts of many investigators over two decades have made to reconstruct the cryo-electron microscopy(cryo-EM)structure of herpesvirus portal vertex and more recently near-atomic structures of two herpesvirus(herpes simplex virus type 1(HSV-1)and Kaposi’s sarcoma-associated herpesvirus(KSHV))portal vertices were reported(McElwee et al.,2018;Gong et al.,2019;Liu et al.,2019).

关键词： vertex shells packing

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中医药防治糖尿病肾病的作用机理研究

陕西中医 2000年第7期21卷 332-334页

作者：武雪萍福建中医学院 350004

糖尿病肾病(Diabetic Nephropath DN)又称糖尿病肾球硬化症,是糖尿病致死致残的主要原因之一。目前认为DN的发生是由遗传因素、代谢紊乱、激素分泌失调、肾血流动力学的改变以及凝血机制、免疫机制失调对肾小球的损害等多因素的结果。... 详细信息

糖尿病肾病(Diabetic Nephropath DN)又称糖尿病肾球硬化症,是糖尿病致死致残的主要原因之一。目前认为DN的发生是由遗传因素、代谢紊乱、激素分泌失调、肾血流动力学的改变以及凝血机制、免疫机制失调对肾小球的损害等多因素的结果。中医无DN病名,但早有对DN的注载;《圣经总录云》:“消渴日久,肾气受伤,肾主水,肾气虚衰,气化失常,开阖不利,水液聚淤体内而成为水肿。”中医学认为DN发病机制为消渴病日久,肾阴亏。搔体受损,肾阳虚衰而致。随着糖尿病患病率的上升,

关键词：糖尿病肾病中医药防治

油茶害虫植物源引诱剂筛选及其诱集的昆虫种群动态

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植物保护 2017年第5期43卷 174-179页

作者：刘俊周德明周国英中南林业科技大学森林有害生物防控湖南省重点实验室经济林培育与保护教育部重点实验室长沙410004

通过16种植物提取物对油茶林昆虫进行诱捕,筛选出2种对油茶害虫引诱效果较好的植物提取物,并在油茶林进行引诱效果及诱集昆虫种群动态的研究。结果表明,2016年4月-8月的引诱试验中,共诱集到昆虫7 455头。其中油茶叶片水提粗提物诱集到... 详细信息

通过16种植物提取物对油茶林昆虫进行诱捕,筛选出2种对油茶害虫引诱效果较好的植物提取物,并在油茶林进行引诱效果及诱集昆虫种群动态的研究。结果表明,2016年4月-8月的引诱试验中,共诱集到昆虫7 455头。其中油茶叶片水提粗提物诱集到昆虫数量最多,达4 368头,诱虫谱较广,诱集到23种昆虫,可以诱集为害油茶林的害虫主要有假眼小绿叶蝉、琼凹大叶蝉、大青叶蝉、白痣广翅蜡蝉、茶角胸叶甲等;其次为油茶乙醇粗提物,诱集到2 078头昆虫,且对八点广翅蜡蝉、小蠹类害虫的诱捕效果最佳。植物提取物作为引诱剂,可为油茶林害虫的发生期、发生量的监测预报提供直接的、客观的依据。

关键词：油茶害虫引诱剂种群动态