Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

作     者：LIU Zhiyi XIANG Jianhai ZHOU Guoying GONG Zuxun 

作者机构：Experimental Marine Biology LaboratoryInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Shanghai Institute of BiochemistryChinese Academy of SciencesShanghai 200031China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2001年第46卷第9期

页      面：766-770,794页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：This work was supported by the National Marine Hi-Tech "863" Project (Grant No. 819-04-08-98) and the "973" Project (Grant No. G1999012007) 

主　　题：gene gun GFP transgenic shrimps. 

摘      要：Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.