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Enhancing Effect of Fe^(2+) on the Formaldehyde Production from Trimethylamine N-oxide Decomposition Catalyzed by the Extract of Harpadon nehereus Kidney

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Journal of Ocean University of China 2011年第1期10卷 67-72页

作者： SUN Yong zhou deqing ZHAO Feng Yellow Sea Fishery Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 P. R. China

The effects of Fe2+ on the trimethylamine N-oxide (TMAO) demethylating activity of the Harpadon nehereus kidney extract were studied in this *** activity of the kidney extract was presumably inhibited by ethylene diamine tetra-acetic acid (EDTA),which indicates that the kidney extract contains an enzyme or enzyme system with metal cations as *** of the kidney extract was enhanced significantly when Fe2+ was added into the model system in *** the concentration of Fe2+ increased,the decomposing rate of TMAO increased rapidly until TMAO decomposed *** activity of the kidney extract was also enhanced by reductant such as ascorbic *** Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) was employed to determine the content of total iron in a number of fishery *** positive correlation between the contents of total iron and endogenous formaldehyde (FA) was found,especially in marine products.

关键词： TMAOase formaldehyde mechanism Fe2+ Harpadon nehereus

Deletion of the waaf gene affects O antigen synthesis and pathogenicity in Vibrio parahaemolyticus from shellfish

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Food Science and Human Wellness 2022年第2期11卷 418-426页

作者： Feng Zhao Guoying Ding Qilong Wang Huihui Du Guosheng Xiao deqing zhou College of biology and food engineering Chongqing Three Gorges UniversityWanzhou 404100China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesLaboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China

Vibrio parahaemolyticus is the main cause of foodborne gastroenteritis,which is widely distributed in shellfish and other *** *** are nonpathogenic,and only a few types,such as serotype O3:K6,are pathogenic,which is also the most prevalent strain in ***,the relationship between this serotype and pathogenicity has yet to be *** waaf gene is located in the O antigen synthesis gene ***,we constructed a waaf gene deletion mutant(i.e.,Δwaaf)of wild-type(WT)which isolated from shellfish serotype O3:K6 via chitin-mediated transformation *** then constructed theΔwaaf complementary strain(i.e.,C-△waaf)via the Escherichia coli S17λpir strain by *** basic physiological characteristics,adhesion to Caco2 cells,and pathogenicity of the WT,△waaf,and C-△waaf strains were *** curves showed no remarkable differences between the WT andΔwaaf ***,theΔwaaf strain non-reactive to O3 antisera and other 12 O-group antisera of ***,the number of flgella and extracellular polysaccharides decreased,the adhesion decreased,and the pathogenicity *** characteristics of the C-△waaf strain were similar to those of the WT strain These results indicated that the waaf gene is vital to the serotype in ***,and changes in O antigen could affect the pathogenicity of this *** study will be helpful to understand the pathogenic mechanism of ***.

关键词： Vibrio parahaemolyticus waaf gene LPS O antigen Pathogenicity

An Efficient Method of Noroviruses Recovery from Oysters and Clams

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Journal of Ocean University of China 2013年第1期12卷 85-90页

作者： zhou deqing MA Liping ZHAO Feng YAO Lin SU Laijin LI Xinguang Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences College of Food Science and Technology Shanghai Ocean University

Noroviruses (NoVs) are widespread causes of nonbacterial gastroenteritis. Outbreaks of NoVs caused diseases are commonly ascribed to the consumption of contaminated shellfish. The concentration and RNA extraction of NoVs are crucial steps of detecting NoVs in shellfish. This study aimed to select a simple, rapid and highly efficient recovery method of NoVs detection with real-time RT-PCR. Four methods of recovering GI.3 and GII.4 NoVs from spiked digestive tissues of oysters and clams, respectively, were compared, of them, the method involving proteinase K and PEG 8000 was found the most efficient. With this method, 9.3% and 13.1% of GI.3 and GII.4 NoVs were recovered from oysters and 9.6% and 12.3% of GI.3 and GII.4 NoVs were recovered from clams, respectively. This method was further used to detect NoVs in 84 oysters (Crassostrea gigas) and 86 clams (Ruditapes philippinarum) collected from 10 coastal cities in China from Jan. 2011 to Feb. 2012. The NoVs isolation rates were 10.47% of clams (9/86) and 7.14% of oysters (6/84). All the detected NoVs belonged to genotype GII. The NoVs recovery method selected is efficient for NoVs detection in oysters and clams.

关键词： norovirus recovery method oyster clam real-time RT-PCR

Histo-blood group antigens in Crassostrea gigas and binding profiles with GⅡ.4 Norovirus

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Journal of Oceanology and Limnology 2018年第4期36卷 1383-1391页

作者： MA Liping LIU Hui SU Laij in ZHAO Feng zhou deqing DUAN Delin Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

Noroviruses(NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens(HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GⅡ.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GⅡ.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected(100%), whereas type Leb(91.67%) and type A(61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006 b and strain-96/96 US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and Ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96 US and strain-2006 b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GⅡ.4 NoV P particles showed that strain-95/96 US and strain-2006 b strongly bound to type A, B, H1, Leb, and Ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and Ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and Ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.

关键词： Crassostrea gigas norovirus histo-blood group antigen binding

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编写《微生物学教程》有感

微生物学通报 2022年第3期49卷 1195-1199页

作者：周德庆复旦大学生命科学学院上海200433

《微生物学教程》获“首届全国教材奖”(二等奖)的消息后,为响应学院领导提出的总结、交流等要求,特撰写了本文。作者以“愿终生能炼成一个称职的微生物学教师”为指导思想,历经半个多世纪的努力,在教书育人的同时,编写了较多的文章和书... 详细信息

《微生物学教程》获“首届全国教材奖”(二等奖)的消息后,为响应学院领导提出的总结、交流等要求,特撰写了本文。作者以“愿终生能炼成一个称职的微生物学教师”为指导思想,历经半个多世纪的努力,在教书育人的同时,编写了较多的文章和书籍,其中的代表作当属《微生物学教程》。该书至今已出了4版,被300余所高等院校选作教材,总印数超百万册。主要特色为:一人独撰,具中国特色,体系稳定,紧跟学科前沿,重视训练学生的长效记忆和战略思维,关心教学方法的改进和交流,以及注重理论联系实际等。作者对教材编写的体会主要是:干一行,爱一行;自认笨,大有益;轻功利,求实绩;勤厚积,方薄发;花可发,柳自荫;青自蓝,更胜蓝。

关键词：微生物学教程编写体会热爱教学教材特色

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应当重视“欲”对美学研究的意义

贵州社会科学 2005年第1期 66-69页

作者：周德清武汉大学湖北武汉430072

中国古代思想特别是儒家思想将“欲”区分为物质之欲和精神之欲 ,同时探讨了“欲”与“礼”“乐”的转化及其审美意义。然而“欲”的审美品格长期以来被美学家严重忽视。应当重视“欲”对美学研究的意义 ,并深思其在现时代的转化及其历... 详细信息

中国古代思想特别是儒家思想将“欲”区分为物质之欲和精神之欲 ,同时探讨了“欲”与“礼”“乐”的转化及其审美意义。然而“欲”的审美品格长期以来被美学家严重忽视。应当重视“欲”对美学研究的意义 ,并深思其在现时代的转化及其历史的、文化的生成之途。

关键词：应当美学研究现时代美学家物质儒家思想精神审美品格审美意义生成

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过程数据驱动的在线开放课程质量评价设计

中国远程教育 2024年第9期44卷 90-100页

作者：周德青杨现民江苏师范大学智慧教育学院徐州221116 江苏师范大学江苏省教育信息化工程技术研究中心徐州221116

“十四五”时期,关注学习过程,重视用户体验,构建与时代背景相适应的在线开放课程质量评价框架与指标,已成为推动在线教育高质量发展的重要命题。本研究从过程视角出发,以数据为导向,关注在线开放课程实际使用情况,首先构建了过程数据... 详细信息

“十四五”时期,关注学习过程,重视用户体验,构建与时代背景相适应的在线开放课程质量评价框架与指标,已成为推动在线教育高质量发展的重要命题。本研究从过程视角出发,以数据为导向,关注在线开放课程实际使用情况,首先构建了过程数据驱动的在线开放课程质量评价框架,该框架包含学习过程数据采集、学习过程数据映射、学习过程数据分析与课程质量等级评价四个流程,以及数据生成、数据采集、数据映射、数据分析、结果输出、质量计算、优化策略七个评价环节;其次设计了过程数据驱动的在线开放课程质量评价指标体系;最后以“中小学教师数据素养”MOOC为例,基于第二期的学习过程数据开展了评价实践,分析了课程存在的实际问题,并从课程内容、学习过程、课程平台环境、学习支持服务四个方面提出在线开放课程质量的优化策略。

关键词：学习过程数据在线开放课程课程质量评价评价框架评价指标体系

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副溶血性弧菌O抗原的分子分型研究

中国食品学报 2015年第4期15卷 147-154页

作者：孟松松赵峰周德庆中国水产科学研究院黄海水产研究所山东青岛266071 上海海洋大学食品学院上海201306

为建立一种针对副溶血性弧菌O抗原进行分子分型的方法,以实验室前期从水产品中分离的6个O群的54株副溶血性弧菌为研究菌株,采用长聚合酶链式反应扩增副溶血性弧菌O抗原合成相关基因,使用3种限制性内切酶(Eco R V,BsmⅠ和XmnⅠ)对扩增产... 详细信息

为建立一种针对副溶血性弧菌O抗原进行分子分型的方法,以实验室前期从水产品中分离的6个O群的54株副溶血性弧菌为研究菌株,采用长聚合酶链式反应扩增副溶血性弧菌O抗原合成相关基因,使用3种限制性内切酶(Eco R V,BsmⅠ和XmnⅠ)对扩增产物进行限制性酶切。经琼脂糖凝胶电泳,NTSYS软件进行聚类分析,结果表明:琼脂糖凝胶电泳和聚类分析均可得到清晰的电泳图和准确的分型。聚类结果显示:经Eco R V、Bsm I和Xmn I 3种限制性内切酶酶切,分别在相似系数为0.72,0.78,0.86时将54株菌分成6个群,且分型结果同副溶血性弧菌的O群血清分型完全吻合。所建立的分子分型方法具有良好的分辨力,产生的条带明亮、清晰,方法操作简便,试验成本低。

关键词：副溶血性弧菌限制性内切酶血清分型O抗原合成相关基因

人工智能赋能乡村教师高质量发展:内涵、知识框架与行进路径

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中国电化教育 2024年第3期 84-91页

作者：周德青穆肃吴玫华南师范大学教育信息技术学院广东广州510631 华南师范大学教育人工智能研究院广东广州510631

优秀乡村教师是乡村教育高质量发展的中坚力量,人工智能赋能乡村教师高质量发展是乡村教育振兴与教育高质量发展的应有之义。现实中乡村教师高质量发展面临内在成长与外生培育的双重困境,即在内乡村教师主动性欠佳,智能教育素养不高;在... 详细信息

优秀乡村教师是乡村教育高质量发展的中坚力量,人工智能赋能乡村教师高质量发展是乡村教育振兴与教育高质量发展的应有之义。现实中乡村教师高质量发展面临内在成长与外生培育的双重困境,即在内乡村教师主动性欠佳,智能教育素养不高;在外乡村教师教研培训过程粗放,人工智能技术应用不深,因此效率效果欠佳。为化解以上困境,研究从目标、路径、结果、过程上梳理了人工智能赋能乡村教师高质量发展的新内涵,提出了涵盖人工智能技术(iK)、教学法(PK)、多学科内容(CK)、乡村文化境脉(XK)以及乡村学生及学习科学(LK)五个知识要素的iPACK-XL框架,并从建设基于iPACKXL的研训课程、探索人工智能技术支持的精准培训模式、应用数据驱动的智能研修平台与工具、构建与优化乡村教师发展的智能画像等四方面提出了利用人工智能赋能乡村教师高质量发展的行进路径,推动构建乡村教师高质量发展的新格局,重塑乡村教师高质量发展的新生态。

关键词：人工智能乡村教师高质量发展 TPACK iPACK-XL