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An axenic strain reveals the responses of Phaeodactylum tricornutum to external organic carbon

Journal of Oceanology and Limnology 2024年第5期42卷 1621-1633页

作者： Zhengfeng ZHU Zhichao HE Jian LI chengxu zhou Yanrong LI Lin ZHANG Xiaohui LI Spiros NAGATHOS Jichang HAN College of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315832China School of Biological and Chemical Engineering Panzhihua UniversityPanzhihua 617000China Ningbo Institute of Oceanography Ningbo 315832China School of Marine Life Ningbo UniversityNingbo 315832China Earth&Life Institute Catholic University of LouvainLouvain-La-Neuve 1348Belgium

The model diatom Phaeodactylum tricornutum is considered a promising source of various high value bioproducts,and developing cultivation processes is crucial for its *** mixotrophy and heterotrophy have been recommended as effective strategies for microalgal cultivation,previous studies on *** have yielded conflicting results in terms of cultivating this *** verify the capacity of this microalga utilizing external organic carbon,both heterotrophic and mixotrophic cultivation with varied carbon sources were performed using an axenic *** results demonstrate that glycerol was the only organic carbon that substantially stimulated the growth of *** in the presence of *** acetate(NaAc)at low concentrations could also promote growth,while at high concentrations led to severe inhibition under mixotrophic *** addition of glucose imposed no appreciable impact on either cell density or biomass concentration,confirming that *** cannot metabolize external ***,a comparative analysis between mixotrophy and autotrophy was performed to reveal the influences of glycerol on the cellular metabolism based on growth performances,biochemical compositions,and chlorophyll fluorescence *** also indicate that the addition of glycerol did not have detrimental effects on the capacity of either pigments biosynthesis or photosynthesis,but enhanced the saturated fatty acids and reduced the unsaturated fatty acids.

关键词： Phaeodactylum tricornutum autotrophy mixotrophy fucoxanthin glycerol

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Diatoms as indicators of environmental change in coastal areas:a case study in Lianjiang coast of East China Sea

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Acta Oceanologica Sinica 2024年第8期43卷 47-57页

作者： Tong Li Jihui Zhang Dongling Li chengxu zhou Chenxi Liu Hao Xu Bing Song Longbin Sha Donghai Academy Ningbo UniversityNingbo 315211China Department of Geography and Spatial Information Techniques Ningbo UniversityNingbo 315211China College of Food Science and Engineering Ningbo UniversityNingbo 315211China State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China

Owing to the significant differences in environmental characteristics and explanatory factors among estuarine and coastal regions,research on diatom transfer functions and database establishment remains *** study analysed diatoms in surface sediment samples and a sediment core from the Lianjiang coast of the East China Sea,together with environmental *** component analysis of the environmental variables showed that sea surface salinity(SSS)and sea surface temperature were the most important factors controlling hydrological conditions in the Lianjiang coastal area,whereas canonical correspondence analysis indicated that SSS and pH were the main environmental factors affecting diatom *** on the modern diatom species–environmental variable database,we developed a diatom-based SSS transfer function to quantitatively reconstruct the variability in SSS between 1984 and 2021 for sediment core HK3 from the Lianjiang coastal *** agreement between the reconstructed SSS and instrument SSS data from 1984 to 2021 suggests that diatombased SSS reconstruction is reliable for studying past SSS variability in the Lianjiang coastal *** low SSS events in AD 2019,2013,and 1999,together with an increased relative concentration of freshwater diatom species and coarser sediment grain sizes,corresponded to two super-typhoon events and a catastrophic flooding event in Lianjiang ***,a diatom-based SSS transfer function for reconstructing past SSS variability in the estuarine and coastal areas of the East China Sea can be further used to reflect the paleoenvironmental events in this region.

关键词： diatom transfer function multivariate statistical analysis environmental variable sea surface salinity

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基于机器感知与学习的空气颗粒物智能检测、识别与预警方法研究综述

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北京工业大学学报 2024年第2期50卷 195-206页

作者：李亚宏周城旭段立娟王思梦顾锞北京工业大学信息学部北京100124

随着空气污染问题的不断加剧,准确检测和及时预警空气颗粒物(particulate matter,PM)的重要性日益突出。传统方法依赖专业设备,不适用于实时检测。与传统方法相比,基于机器感知与学习的方法体现出技术优势,具有可实时检测、准确性高等... 详细信息

随着空气污染问题的不断加剧,准确检测和及时预警空气颗粒物(particulate matter,PM)的重要性日益突出。传统方法依赖专业设备,不适用于实时检测。与传统方法相比,基于机器感知与学习的方法体现出技术优势,具有可实时检测、准确性高等优点。因此,对近几年的基于机器感知与学习的PM智能检测、识别与预警方法进行详细综述。首先,对PM的标准和来源进行介绍;然后,从检测、识别和预警这3个方面详细总结了各类方法,并对比各方法的特点和性能,其中,基于机器学习和深度学习的方法在各研究中取得了较大进展;最后,总结全文主要内容,并提出当前领域面临的挑战以及未来的重点研究方向。未来的研究应该继续关注技术创新和数据质量,以实现更好的空气质量监测和管理。

关键词：空气污染机器感知颗粒物(particulate matter,PM) 智能检测空气质量指数(air quality index,AQI) 深度神经网络

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Overview of Gait Synthesis for the Humanoid COMAN

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Journal of Bionic Engineering 2017年第1期14卷 15-25页

作者： chengxu zhou Xin Wang Zhibin Li Nikos Tsagarakis Department of Advanced Robotics lstituto Italiano di Tecnologia Via Morego 30 16163 Genova Italy Shenzhen Academy of Aerospace Technology Shenzhen 518057 China School of Informatics University of Edinburgh 10 Crichton St Edinburgh EH8 9AB UK

This paper focuses on the developments of a generic gait synthesis for the humanoid robot COMAN. Relying on the essential Gait Pattern Generator （GPG）, the proposed synthesis offers enhanced versatilities for the locomotion under different purposes, and also provides the data storage and communication mechanisms among different modules. As an outcome, we are able to augment new abilities for COMAN by integrating new control modules and software tools at a cost of very few modifications. Moreover, foot placement optimization is introduced to the GPG to optimize the gait parameter references in order to meet the robot＇s natural dynamics and kinematics, which enhances the synthesis＇s robustness while it＇s being implemented on real robots. We have also presented a practical approach to generate pelvis motion from CoM references using a simplified three-point-mass model, as well as a straightforward but effective idea for the state estimation using the sensory feedback. Three physical experiments were studied in an increasing complexity to demonstrate the effectiveness and successful implementation of the proposed gait synthesis on a real humanoid system.

关键词： humanoid robots bipedal locomotion gait synthesis dynamic walking

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A Real-Time Planning and Control Framework for Robust and Dynamic Quadrupedal Locomotion

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Journal of Bionic Engineering 2023年第4期20卷 1449-1466页

作者： Jun Li Haibo Gao Yuhui Wan Haitao Yu chengxu zhou State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin150001China School of Mechanical Engineering University of LeedsLeedsLS29JTUK

Legged locomotion poses significant challenges due to its nonlinear,underactuated and hybrid dynamic *** challenges are exacerbated by the high-speed motion and presence of aerial phases in dynamic legged locomotion,which highlights the requirement for online planning based on current states to cope with uncertainty and *** article proposes a real-time planning and control framework integrating motion planning and whole-body *** the framework,the designed motion planner allows a wider body rotation range and fast reactive behaviors based on the 3-D single rigid body *** addition,the combination of a Bézier curve based trajectory interpolator and a heuristic-based foothold planner helps generate continuous and smooth foot *** developed whole-body controller uses hierarchical quadratic optimization coupled with the full system dynamics,which ensures tasks are prioritized based on importance and joint commands are physically *** performance of the framework is successfully validated in experiments with a torque-controlled quadrupedal robot for generating dynamic motions.

关键词： Bionic robot Legged locomotion Motion planning Whole-body control

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Isolation of Chloroplasts from Marine Microalga Isochrysis galbana Parke for Their Lipid Composition Analysis

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Journal of Ocean University of China 2022年第1期21卷 225-235页

作者： ZHONG Ye LI Yanrong XU Jilin CAO Jiayi zhou chengxu YAN Xiaojun Key Laboratory of Applied Marine Biotechnology Ningbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China Fujian Baozhi Aquatic Science and Technology Co. Ltd.Zhangzhou 363503China Ningbo Institute of Oceanography Ningbo 315211China

Marine microalga Isochrysis galbana is an important feed species with a high nutritional *** from other uni-cellular algae,its cell contains two chloroplasts which are the major sites for lipid ***,we optimized a chloroplast isola-tion approach suitable for the isolation of *** chloroplasts and determined the purity and integrity of the isolated chloroplasts through microscopic observations and enzyme activity *** chloroplast lipids were analyzed with a ultrahigh-performance li-quid chromatography-Q Exactive Orbitrap-mass *** newly developed isolation approach is simple and reliable to isolate chloroplasts with high integrity and *** average yield of intact chloroplasts was 15.3%±0.1%.Glycolipids and acyl-glycerols were the main chloroplast *** accounted for 56.6%of chloroplast ***(DGDG),monogalactosyldiacylglycerol(MGDG)and sulfoquinovosyldiacylglycerol(SQDG)were the main *** fatty acyl R1/R2 were mostly 18:4/16:1,18:3/16:1 and 18:4/18:5 in DGDGs,14:0/18:4,18:4/18:5,18:4/18:4 and 18:3/18:4 in MGDGs and 16:0/14:0,16:0/18:3,and 18:4/18:3 in *** addition,diacylglycerol(DAG)was the most abundant acylglycerols;the content of 22:6/18:4-DAG was the *** was a little amount of glycosphingolipid(GSL)in ***(DGMG),monogalactosylmonoglyceride(MGMG),sulfoquinovosylmonoacylglycerol(SQMG),monoglyceride(MAG),phospholi-pids(PLs),ceramide(Cer)and betaine lipids were nearly undetectable in *** fatty acid proportions of DGDGs,MGDGs,SQDGs,DAGs,triglycerides(TAGs)and GSLs were either higher or lower than or similar to those of ***,our isolation approach is applicable to many aspects of chloroplast biology,and may offer a reference for the isolation of chloroplasts from other marine microalgae.

关键词： Isochrysis galbana chloroplast isolation method lipid analysis chloroplast lipid

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Zooplankton community size-structure change and mesh size selection under the thermal stress caused by a power plant in a semi-enclosed bay

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Acta Oceanologica Sinica 2020年第8期39卷 62-70页

作者： Qianwen Shao Yifeng Zhu Meixia Dai Xia Lin chengxu zhou Xiaojun Yan Key Laboratory of Applied Marine Biotechnology School of Marine ScienceNingbo UniversityNingbo 315832China Ningbo Institute of Oceanography Ningbo 315832China

Zooplankton samples were collected using 505, 160 and 77 μm mesh nets around a power plant during four seasons in 2011. We measured total length of zooplankton and divided zooplankton into seven size classes in order to explore how zooplankton community size-structure might be altered by thermal discharge from power plant. The total length of zooplankton varied from 93.7 to 40 074.7 μm. The spatial distribution of mesozooplankton(200-2 000 μm) populations were rarely affected by thermal discharge, while macro-(2 000-10 000 μm)and megalo-zooplankton(>10 000 μm) had an obvious tendency to migrate away from the outfall of power ***, zooplankton community tended to become smaller and biodiversity reduced close to power ***, we compared the zooplankton communities in three different mesh size nets. Species richness,abundance, evenness index and Shannon-Wiener diversity index of the 505 μm mesh size were significantly lower than those recorded from the 160 and 77 μm mesh size. Average zooplankton abundance was highest in the 77 μm mesh net((27 690.0±1 633.7) ind./m^3), followed by 160 μm mesh net((9 531.1±1 079.5) ind./m^3), and lowest in 505 μm mesh net((494.4±104.7) ind./m^3). The ANOSIM and SIMPER tests confirmed that these differences were mainly due to small zooplankton and early developmental stages of zooplankton. It is the first time to use the 77 μm mesh net to sample zooplankton in such an environment. The 77 μm mesh net had the overwhelming abundance of the copepod genus Oithona, as an order of magnitude greater than recorded for 160 μm mesh net and 100% loss through the 505 μm mesh net. These results indicate that the use of a small or even multiple sampling net is necessary to accurately quantify entire zooplankton community around coastal power plant.

关键词： zooplankton coastal power plant temperature elevation size class community structure mesh size selection

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三角褐指藻中岩藻黄素的分离纯化工艺研究

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中国海洋药物 2024年第4期43卷 19-27页

作者：朱军旺叶月美周成旭张金荣宁波大学食品与药学学院浙江宁波315832

目的探索三角褐指藻(Phaeodactylum tricornutum)冻干藻粉中岩藻黄素的分离及纯化方法,为深入研究三角褐指藻中岩藻黄素的工业化制备提供技术方法。方法培养三角褐指藻并获得冻干藻粉,70%乙醇进行提取,ODS开放柱层析分离,乙醇沉淀法... 详细信息

目的探索三角褐指藻(Phaeodactylum tricornutum)冻干藻粉中岩藻黄素的分离及纯化方法,为深入研究三角褐指藻中岩藻黄素的工业化制备提供技术方法。方法培养三角褐指藻并获得冻干藻粉,70%乙醇进行提取,ODS开放柱层析分离,乙醇沉淀法进行纯化得到岩藻黄素。用电喷雾质谱法(ESI-MS)和核磁共振波谱法(1H NMR)对纯化得到的岩藻黄素进行结构表征。结果 ODS柱层析分离过程中,绝大多数岩藻黄素在80%乙醇条件下成功洗脱,回收率为(88.02±2.30)%。乙醇沉淀的最佳工艺为醇沉含醇量50%、4℃静置24 h。在此乙醇析出条件下,岩藻黄素的回收率为(86.84±0.99)%,纯度为(95.79±1.06)%。分离纯化得到的岩藻黄素经质谱和核磁共振波谱鉴定,其结构为全反式岩藻黄素。结论该工艺为全流程绿色高效分离纯化技术,工艺简单,岩藻黄素收率高,为进一步研究以三角褐指藻为原料工业化生产高纯度岩藻黄素提供了技术方法,为岩藻黄素产品在食品和医药领域的开发提供了理论依据。

关键词：岩藻黄素三角褐指藻 ODS柱层析乙醇析出分离纯化

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Comparative study of brine shrimp bioassay-based toxic activities of three harmful microalgal species that frequently blooming in aquaculture ponds

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Journal of Oceanology and Limnology 2018年第5期36卷 1697-1706页

作者： YANG Xiaoqian WEN Xin zhou chengxu ZHU Xiaojuan MENG Ran LUO Qijun YAN Xiaojun School of Marine Science Ningbo UniversityNingbo 315211China Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo UniversityNingbo 315211China Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center Ningbo UniversityNingbo 315211China Senior High School of Yudu Ganzhou 343200China

Typical harmful micro-algal species constantly occurred in high density in marine aquaculture ponds in Xiangshan and Sanmen Bay, Zhejiang Province. Fates of the microalgal cells influenced by activity of the cultured animals largely determined the ef fects of the harmful microalgae. However, it is difficult to detect the in situ process. In this paper, toxic activities of three harmful microalga, namely P rymnesium parvum, Pleurochrysis elongata, Karlodinium veneficum, which were isolated from the local ponds, were comparatively studied based on brine shrimp toxic bioassays. Diff erent lethal activities of live cells, cell debris, cellular extracts, and cell free mediums prepared by different process were analyzed. The results showed that,(1) all of the three microalgal species had density and time dependent lethal ef fects on A rtermia nauplii, while P. parvum was the most toxic one and had acute lethal eff ects in 5 h. No such acute lethal eff ects were observed in P. elongata or K. veneficum;(2) live cells, cell debris and cellular extracts of P. parvum had the same lethal pattern. Prymnesins, toxin from P. parvum, is probably not exotoxic active; For P. elongata, toxic activity mainly came from live cells and cell debris; For K. veneficum, toxic activity was relatively lower compared with the other two species. However, Karlotoxin, toxin from K. veneficum, is exotoxic active. Physical disturbance triggered K. veneficum cells actively releasing toxins, which made it an active predator.

关键词： Prymnesium parvum Pleurochrysis elongata Karlodinium veneficum Artemia nauplii toxic activity

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Changes of pigments and lipids composition in Haematococcus pluvialis vegetative cell as affected by monochromatic red light compared with white light

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Journal of Oceanology and Limnology 2018年第6期36卷 2257-2267页

作者： ZHU Xiaojuan LUO Jie zhou chengxu WANG Jianyuan MENG Ran XU Jilin CHEN Juanjuan LUO Qijun YAN Xiaojun School of Marine Science Ningbo University Department of Marine Drug and Biological Products Ningbo Institute of Oceanography Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center Ningbo University Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University

Much attention has been paid on studies of astaxanthin accumulation process in H aematococcus pluvialis industry. However, growth of H. pluvialis in motile vegetative stage is still the most important and problematic part in the whole cultivation process, such as low growth rate and cell yields. Motile vegetative cells are extremely sensitive to various stresses which make it difficult to maintain the cells of this state to grow. Previous reports showed that motile vegetative cells may have higher biomass yields ifapplied monochromatic red light. However, metabolic responses of these cells are not completely understood, which constraints application of this illumination protocol in industry. The aim of this study was to examine how critical biochemical changes of H. pluvialis motile vegetative cells were af fected by red light when compared with white light. Variation of photosynthetic pigments composition and lipids were mainly studied. By comparing growth process of cultures in red light and white light, prominent variation of pigments composition and lipids changes were observed. The results showed that, even though cell proliferation was the same during exponential growth phase, variation of photosynthetic pigment composition and lipids occurred. The final biomass of cell number was higher in red light group than in white light group. The variations were significant different. Increase or decrease of major photosynthetic membrane lipids to some extent did not influence photosynthesis of the vegetative cells during this phase. However, vegetative cells under polychromatic white light other than monochromatic red light need further metabolic process to adjust its pigment composition and lipids, possibly this is energetically and biochemically unfavorable for motile vegetative cells to growth under white light, a light condition normally not considered as a stress.

关键词： Haematococcus pluvialis light quality photosynthetic pigments neutral lipid photosynthetic membrane lipids

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