检索结果-南通市图书馆

The evolution and functional divergence of 10 Apolipoprotein D-like genes in Nilaparvata lugens

在线全文

学校读者我要写书评

暂无评论

Insect Science 2024年第1期31卷 91-105页

作者： Jia-Bao Lu Peng-Peng Ren Qiao Li Fang He zhong-Tian Xu Sai-nan Wang Jian-Ping Chen Jun-Min Li Chuan-Xi Zhang Citrus Research Institute Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for CitrusChongqingChina Technology Center of Wuhan Customs District HubeiChina Institute of Insect Science Zhejiang UniversityHangzhouChina

Apolipoprotein D(ApoD),a member of the lipocalin superfamily of proteins,is involved in lipid transport and stress *** only a single copy of the ApoD gene is found in humans and some other vertebrates,there are typically several ApoD-like genes in *** date,there have been relatively few studies that have examined the evolution and functional differentiation of ApoD-like genes in insects,particularly hemi-metabolous *** this study,we identified 10 ApoD-like genes(NlApoD1−10)with distinct spatiotemporal expression patterns in Nilaparvata lugens(BPH),which is an important pest of ***1−10 were found to be distributed on 3 chromosomes in a tandem array of NlApoD1/2,NlApoD3−5,and NlApoD7/8,and show sequence and gene structural divergence in the coding regions,indicating that multiple gene duplication events occurred during *** analysis revealed that NlApoD1−10 can be clustered into 5 clades,with NlApoD3−5 and NlApoD7/8 potentially evolving exclusively in the Delphacidae *** screening using an RNA interference approach revealed that only NlApoD2 was essential for BPH development and survival,whereas NlApoD4/5 are highly expressed in testes,and might play roles in ***,stress response analysis revealed that NlApoD3−5/9,NlApoD3−5,and NlApoD9 were up-regulated after treatment with lipopolysaccharide,H2O2,and ultraviolet-C,respectively,indicating their potential roles in stress resistance.

关键词： Apolipoprotein D BPH evolution functional divergence gene duplication NIApoD

呼吸监护病区患者下呼吸道分离革兰阴性杆菌连续5年耐药性监测

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国感染与化疗杂志 2007年第5期7卷 367-371页

作者：叶枫钟淑卿袁锦屏杨灵钟南山广州市呼吸疾病研究所广州510120

目的探讨本所呼吸监护病区(RICU)患者下呼吸道分离革兰阴性杆菌的耐药变迁,指导临床合理使用抗菌药。方法2000-2004年从RICU患者下呼吸道分离的非重复的1047株革兰阴性杆菌采用Kirby-Bauer法进行药敏试验,数据分析采用WHONET5.3软件。... 详细信息

目的探讨本所呼吸监护病区(RICU)患者下呼吸道分离革兰阴性杆菌的耐药变迁,指导临床合理使用抗菌药。方法2000-2004年从RICU患者下呼吸道分离的非重复的1047株革兰阴性杆菌采用Kirby-Bauer法进行药敏试验,数据分析采用WHONET5.3软件。结果最常见的病原菌为铜绿假单胞菌(42.9%)、嗜麦芽窄食单胞菌(17.1%)、鲍曼不动杆菌(10.0%)和肺炎克雷伯菌(6.5%)。敏感率较高的抗菌药是头孢他啶(50%～74%)、阿米卡星(33.3%～81.0%)、哌拉西林-三唑巴坦(30.4%～64.6%)、头孢哌酮-舒巴坦(33.5%-47.5%),亚胺培南的敏感率波动性较大。对嗜麦芽窄食单胞菌敏感性较高的药物是头孢哌酮-舒巴坦(47.2%～78.6%)和替卡西林-克拉维酸(28.3%～86.6%)。鲍曼不动杆菌对亚胺培南的敏感率始终保持在90%以上,头孢哌酮-舒巴坦的敏感率(18.2%～68.2%)与阿米卡星(31.8%～76.2%)相近。肺炎克雷伯菌对亚胺培南和头孢吡肟的敏感率最高,分别为92.9%～100%和55.6%～80.0%,哌拉西林-三唑巴坦的敏感率由58.3%降至21.7%。肺炎克雷伯菌产ESBLs株检出率由2002年的11.1%上升至2004年的47.8%。结论5年来本研究监测的细菌耐药率明显升高,加强耐药性监测,合理使用抗菌药物非常重要。

关键词：革兰阴性杆菌耐药监测耐药性微生物

呼吸病区下呼吸道革兰阴性杆菌连续5年耐药性监测

在线全文

学校读者我要写书评

暂无评论

中国感染与化疗杂志 2009年第2期9卷 129-133页

作者：叶枫钟淑卿袁锦屏杨灵钟南山广州呼吸疾病研究所 510120

目的探讨本所呼吸病区患者下呼吸道分离革兰阴性杆菌的耐药变迁,指导临床合理使用抗菌药物。方法2003—2007年从呼吸病区患者下呼吸道分离的非重复的1 709株革兰阴性杆菌,采用Kirby-Bauer法进行药敏试验,数据分析采用WHONET5.4软件。结... 详细信息

目的探讨本所呼吸病区患者下呼吸道分离革兰阴性杆菌的耐药变迁,指导临床合理使用抗菌药物。方法2003—2007年从呼吸病区患者下呼吸道分离的非重复的1 709株革兰阴性杆菌,采用Kirby-Bauer法进行药敏试验,数据分析采用WHONET5.4软件。结果最常见的病原菌为铜绿假单胞菌(43.7%)、肺炎克雷伯菌(11.1%)、嗜麦芽窄食单胞菌(9.2%)和鲍曼不动杆菌(9.0%)。铜绿假单胞菌对头孢哌酮-舒巴坦的耐药率最低,其次是阿米卡星(10.1%～27.7%)和美罗培南(17.3%～31.8%)。嗜麦芽窄食单胞菌对头孢哌酮-舒巴坦(5.9%～17.9%)和左氧氟沙星(7.1%～17.4%)的耐药率较低。亚胺培南和美罗培南对鲍曼不动杆菌保持较强的抗菌活性,头孢哌酮-舒巴坦对该菌的耐药率(0～22.2%)明显低于替卡西林-克拉维酸(17.4%～50.0%)和哌拉西林-他唑巴坦(16.7%～61.5%)。肠杆菌科细菌对亚胺培南和美罗培南仍高度敏感,对氟喹诺酮类药物的耐药率较高,且各品种间呈交叉耐药。肺炎克雷伯菌对头孢吡肟(0～32.6%)和头孢哌酮-舒巴坦(2.4%～38.1%)的耐药率明显低于第三代头孢菌素(11.9%～69.0%)。肺炎克雷伯菌产ESBLs株检出率由2003年的16.1%上升至2005年的76.7%,2007年降至28.6%,而大肠埃希菌产ESBLs株检出率则由2003年的45.5%上升至2007年的80.6%。产ESBLs菌株对大多数β内酰胺类抗生素高度耐药。结论细菌耐药率明显升高,加强耐药性监测,合理使用抗菌药物非常重要。

关键词：革兰阴性杆菌耐药监测耐药性

不同干预措施对犬急性肺损伤早期呼吸应答和呼吸做功的影响

在线全文

学校读者我要写书评

暂无评论

南方医科大学学报 2007年第3期27卷 340-344页

作者：陈新许睿陈荣昌岑燕遗钟南山南方医科大学珠江医院呼吸内科广东广州510282 南方医科大学珠江医院麻醉科广东广州510282 广州医学院第一附属医院广州呼吸疾病研究所广东广州510120

目的探讨早期应用控制性高浓度氧疗、持续气道内正压(CPAP)、双水平气道内正压(BiPAP)不同的干预措施对急性肺损伤(ALI)早期呼吸应答和呼吸做功的影响。方法24只杂种犬在制作油酸ALI模型成功后(PaO2/FiO2≤300mmHg),保持自主呼吸,随机进... 详细信息

目的探讨早期应用控制性高浓度氧疗、持续气道内正压(CPAP)、双水平气道内正压(BiPAP)不同的干预措施对急性肺损伤(ALI)早期呼吸应答和呼吸做功的影响。方法24只杂种犬在制作油酸ALI模型成功后(PaO2/FiO2≤300mmHg),保持自主呼吸,随机进入:(1)控制性高浓度氧疗组(n=8);(2)CPAP组(n=8);(3)BiPAP组(n=8)。连续记录并计算正常、ALI早期、干预后1～4h内呼吸应答和呼吸做功各项生理指标。结果在降低呼吸频率和浅快呼吸呼吸指数上,BiPAP组效果最明显(P0.05),维持在ALI/ARDS发生后的通气水平。在潮气量、平均吸气流量指标的改善上,BiPAP组效果最显著,其次是CPAP组和氧疗组。3组在吸气时间占呼吸周期比值上无显著性差异(P>0.05)。在跨膈压峰值上,BiPAP组降低最明显,其次是CPAP组。CPAP组和BiPAP组可有效地抵消内源性PEEP(P0.05)。在降低吸气做功上,BiPAP组最显著,其次是CPAP组,氧疗组呼吸功耗与干预前比较无显著性差异。CPAP组(P<0.01)和BiPAP组(P<0.05)有效地降低了克服内源性PEEP成分做功的比例。结论BiPAP和CPAP对于缓解呼吸窘迫、减少呼吸功耗、改善呼吸应答均具有积极作用,显著延缓了ALI/ARDS的病情恶化,其中BiPAP效果更为显著。

关键词：急性肺损伤急性呼吸窘迫综合征呼吸应答呼吸做功控制性氧疗持续气道内正压双水平气道内正压

广州地区3500株革兰阴性细菌的分布特征及耐药性特点

在线全文

学校读者我要写书评

暂无评论

第一军医大学学报 2005年第2期25卷 132-138页

作者：肖庆忠苏丹虹江洁华钟南山广州医学院第一附属医院检验科广东广州510120 广州医学院第一附属医院呼吸疾病研究所广东广州510120

目的了解广州地区医院感染常见革兰阴性细菌分布特征和耐药特点.方法2001年7月～2003年8月,用Kirby-Bauer法检测了从广州地区13家医院分离获得的3 500株革兰阴性细菌对临床常用15～21种抗生素的药敏结果,并按美国临床实验标准委员会200... 详细信息

目的了解广州地区医院感染常见革兰阴性细菌分布特征和耐药特点.方法2001年7月～2003年8月,用Kirby-Bauer法检测了从广州地区13家医院分离获得的3 500株革兰阴性细菌对临床常用15～21种抗生素的药敏结果,并按美国临床实验标准委员会2000年判断标准进行判断,用WHONET-5软件分析数据.结果分离出的3 500株革兰阴性菌中前3位细菌依次是大肠埃希菌(Escherichia coli,ECO)1 244株(占35.5%)、肺炎克雷伯菌(Klebsiellapneumoniae,Kpn)900株(占25.7%)和铜绿假单胞菌(Pseudomonas aeruginosa,Pae)547株(占15.6%).广州地区临床分离革兰阴性菌中产超广谱β内酰胺酶(extended-spectrumbeta-lactamases,ESBLs)菌株的总检出率为31.0%(1 084/3 500),在各种常见菌属中的检出率分别为:大肠埃希菌为38.7%(482/1 244)、肺炎克雷伯菌为37.9%(341/900)、铜绿假单胞菌为5.3%(29/547)、阴沟肠杆菌为55.2%(117/212)、不动杆菌属为8.2%(17/208)、其他肠杆菌属为27.7%(53/191)、嗜麦芽窄食单胞菌为33.3%(37/117)、变形杆菌属为9 2%(8/87).从呼吸道标本中分离获得革兰阴性细菌最多,为1 463株(占41.8%);其次为泌尿道标本,为943株(占26.9%).耐药性分析显示:革兰阴性杆菌对临床常用抗生素的总体耐药率最低为亚胺培南(8.7%),其次为头孢哌酮/舒巴坦(13.3%),而最高为氨苄西林(90.5%),其次为奈啶酸(69 3%).亚胺培南对广州地区临床分离的大肠埃希氏菌、肺炎克雷伯氏菌、阴沟肠杆菌、不动杆菌属、其他肠杆菌属和变形杆菌属的耐药率最低,分别为1.1%、0.5%、0.6%、3.2%、0.8%和0%,而头孢哌酮/舒巴坦对广州地区临床分离的铜绿假单胞菌和嗜麦芽窄食单胞菌的耐药率最低,分别为10.8%和15.9%,大部分细菌呈多重耐药.产ESBLs菌株对15～21种临床常用抗菌药物的耐药率均显著高于不产ESBLs菌株(P＜0.05).结论细菌耐药性仍是目前广州地区临床用药最为严重的问题,尤其是产ESBLs菌株有上升趋势.亚胺培南和头孢哌酮/舒巴坦分别对临床分离革兰阴性菌株有较好的抗菌活性,可以作为广州地区临床经验用药的候选药物.

关键词：革兰氏阴性菌抗药性微生物交叉感染细菌分布 β内酰胺酶类

Recanalization of anastomotic occlusion following rectal cancer surgery using a rendezvous endoscopic technique with transillumination:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第37期30卷 4149-4155页

作者： Jun Chi Guang-Yu Luo Hong-Bo shan Jun-zhong Lin Xiao-Jun Wu Jian-Jun Li Department of Endoscopy Sun Yat-Sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina State Key Laboratory of Oncology in South China Guangzhou 510060Guangdong ProvinceChina Guangdong Provincial Clinical Research Center for Cancer Guangzhou 510060Guangdong ProvinceChina Department of Colorectal Surgery Sun Yat-Sen University Cancer CenterGuangzhou 510060Guangdong ProvinceChina

BACKGROUND Colorectal anastomotic occlusion is a serious complication of colorectal cancer *** several treatment strategies have been proposed,the mana-gement of anastomotic occlusion remains *** this report,we present a case of anastomotic occlusion recanalization performed using a novel technique involving two endoscopes,one for radial incision and the other serving as a guide *** novel technique offers significant advantages in terms of operational feasibility,reduced invasiveness,rapid recovery,and shortened hospital *** SUMMARY A 37-year-old man underwent low anterior resection and prophylactic double-lumen ileostomy for rectal cancer in June,*** months later,complete anastomotic occlusion was observed on ***,we developed a novel atresia recanalization *** endoscopes were placed,one through the colonic anastomosis and the other through the anus.A radial incision was successfully made from the colonic side,guided by the light of the endoscope from the anal *** recanal-ization was performed within 20 *** weeks after recanalization,colonoscopy revealed that the diameter of the colorectal anastomosis was approximately 16 mm and the patient therefore underwent stoma reversal in *** the follow-up period of approximately one year,the patient remained well and no stenosis or obstruction symptoms were *** Endoscopic atresia recanalization of colorectal anastomotic occlusion assisted by an opposing light source is safe and effective.

关键词： Endoscopy Atresia recanalization Anastomotic occlusion Light source Case report

Surface engineering on MnO_(2)nanorods by La single atoms to accelerate oxygen reduction kinetics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2024年第9期43卷 4302-4311页

作者： Zhang-Long He Liu-Qi Wang Min Jiang Jia-nan Xie shan Liu Jin-Can Ren Rui Sun Wen-Bin Lv Wei-Bin Guo Yu-Ling Liu Bing Li Qi Liu Hao He School of Materials Science and Engineering Changsha University of Science and TechnologyChangsha 410000China School of Materials Science and Engineering Nanjing University of Science and TechnologyNanjing 210094China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Department of Physics City University of Hong KongHong Kong 999077China

Surface engineering,which modulates the electronic structure and adsorption/desorption properties of electrocatalysts,is one of the key strategies for improving the catalytic ***,we demonstrate a facile solid-phase reaction for surface engineering of MnO_(2)to boost the oxygen reduction *** reaction with surface hydroxy groups,La single atoms with loading amount up to 2.7 wt%are anchored onto a-MnO_(2)*** surface engineering,the oxygen reduction reaction(ORR)kinetics is significantly improved with the half-wave potential from 0.70 to 0.84 V,the number of transferred electrons from 2.5 to 3.9 and the limiting current density from 4.8 to 6.0 mA·cm^(-2).In addition,the catalyst delivers superior discharge performance in both alkaline and neutral metal–air *** functional theory(DFT)calculations reveal that atomic La modulates the surface electronic configuration of MnO_(2),reduces its d-band center and thus lowers the OOH*and O*reaction energy *** work provides a new route for rational design of highly active electrocatalyst and holds great potential for application in various catalytic reactions.

关键词： Surface engineering Single atoms Electron injection Oxygen reduction reaction Metal-air batteries

N-acetylcysteine ameliorates erectile dysfunction in rats with hyperlipidemia by inhibiting oxidative stress and corpus cavernosum smooth muscle cells phenotypic modulation

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2024年第1期26卷 99-106页

作者： Wei Ding Jun-Hong Fan Li-Ren zhong nan-Xiong Wang Lu-Hao Liu Hai-Bo Zhang Li Wang Ming-Qiang Wang Bing-Lin He An-Yang Wei Department of Urology The First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyang 550002China Department of Urology Guangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhou 510000China Department of Urology Nanfang HospitalSouthern Medical UniversityGuangzhou 510000China Department of Urology Shenzhen Immigration Inspection General Station HospitalShenzhen 518000China Department of Organ Transplantation The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510000China Department of Endocrinology The First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyang 550002China

Hyperlipidemia is a major risk factor for erectile dysfunction(ED).Oxidative stress and phenotypic modulation of corpus cavernosum smooth muscle cells(CcSMCs)are the key pathological factors of ED.N-acetylcysteine(NAC)can inhibit oxidative stress;however,whether NAc can alleviate pathological variations in the corpus cavernosum and promote erectile function recovery in hyperlipidemic rats remains unclear.A hyperlipidemia model was established using 27 eight-week-old male Sprague-Dawley(SD)rats fed a high-fat and high-cholesterol diet(hyperlipidemic rats,HR).In addition,9 male SD rats were fed a normal diet to serve as controls(NC).HR rats were divided into three groups:HR,HR+normal saline(NS),and HR+NAC(n=9 for each group;NS or NAc intraperitoneal injections were administered daily for 16 weeks).Subsequently,the lipid profiles,erectile function,oxidative stress,phenotypic modulation markers of ccsMCs,and tissue histology were *** experimental results revealed that erectile function was significantly impaired in the HR and HR+NS groups,but enhanced in the HR+NAC *** lipid levels,over-activated oxidative stress,and multi-organ lesions observed in the HR and HR+NS groups were improved in the HR+NAC ***,the HR group showed significant phenotypic modulation of CCSMCs,which was also inhibited by NAC *** report focuses on the therapeutic effect of NAc in restoring erectile function using a hyperlipidemic rat model by preventing CcSMC phenotypic modulationand attenuating oxidativestress.

关键词： erectile dysfunction hyperlipidemia N-acetylcysteine oxidative stress henotypic modulation

MH-STRALP:A scoring system for prognostication in patients with upper gastrointestinal bleeding

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Surgery 2024年第3期16卷 790-806页

作者： Jun-nan Hu Fei Xu Ya-Rong Hao Chun-Yan Sun Kai-Ming Wu Yong Lin Lan zhong Xin Zeng Department of Gastroenterology Shanghai East HospitalTongji University School of MedicineShanghai 200120China Department of Gastroenterology Shanghai Changzheng HospitalNavy Military Medical UniversityShanghai 200003China

BACKGROUND Upper gastrointestinal bleeding(UGIB)is a common medical emergency and early assessment of its outcomes is vital for treatment *** To develop a new scoring system to predict its *** In this retrospective study,692 patients with UGIB were enrolled from two cen-ters and divided into a training(n=591)and a validation cohort(n=101).The clinical data were collected to develop new prognostic prediction *** en-dpoint was compound outcome defined as(1)demand for emergency surgery or vascular intervention,(2)being transferred to the intensive care unit,or(3)death during *** models’predictive ability was compared with previously esta-blished scores by receiver operating characteristic(ROC)*** Totally 22.2%(131/591)patients in the training cohort and 22.8%(23/101)in the validation cohort presented poor *** on the stepwise-forward Lo-gistic regression analysis,eight predictors were integrated to determine a new post-endoscopic prognostic scoring system(MH-STRALP);a nomogram was de-termined to present the *** with the previous scores(GBS,Rock-all,ABC,AIMS65,and PNED score),MH-STRALP showed the best prognostic prediction ability with area under the ROC curves(AUROCs)of 0.899 and 0.826 in the training and validation cohorts,*** to the calibration cur-ve,decision curve analysis,and internal cross-validation,the nomogram showed good calibration ability and net clinical benefit in both *** removing the endoscopic indicators,the pre-endoscopic model(pre-MH-STRALP score)was ***,the pre-MHSTRALP score showed better predictive value(AUROCs of 0.868 and 0.767 in the training and validation cohorts,respectively)than the other pre-endoscopic *** The MH-STRALP score and pre-MH-STRALP score are simple,convenient,and accurate tools for prognosis prediction of UGIB,and may be applied for early decision on its management strategies.

关键词： Upper gastrointestinal bleeding Prognosis prediction Retrospective study Nomogram Post-endoscopic model Pre-endoscopic model