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Blocking effects of siRNA on VEGF expression in human colorectal cancer cells

World Journal of Gastroenterology 2010年第9期16卷 1086-1092页

作者： Yu Yin,Li-Yu Cao,Wen-Qing Wu,Hao Li,Yan Jiang,Hong-Fu zhang,Department of Pathology,anhui Medical University,Hefei 230032,anhui Province,China Wen-Qing Wu,Department of Pathology,anhui Provincial Hospital,Hefei 230001,anhui Province,China Department of Pathology Anhui Medical University Hefei 230032 Anhui Province China. caoliyu@***

AIM:To investigate the expression of vascular endothelial cell growth factor (VEGF) and its receptors Fmslike tyrosine kinase 1 (FLT-1) and fetal liver kinase 1 (FLK-1) in colorectal carcinoma (CRC),and the blocking effects of small interfering RNAs (siRNAs) on VEGF expression in human colorectal cancer HCT116 ***:Immunohistochemical staining for VEGF,FLT-1 and FLK-1 proteins was performed in 82 cases of CRC and 14 normal colorectal mucosae.A siRNA targeting VEGF was synthesized and transfected into HCT116 cells using lipofectamine *** staining and Western blotting analyses were performed to detect the expression of VEGF *** suppressive effect of the siRNA on cell proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltertrazolium bromide (MTT) *** apoptosis was detected using flow cytometry (FCM).RESULTS:The expression of VEGF,FLT-1 and FLK-1 in tumor tissues was significantly higher than that in normal tissues (P=0.008,P=0.000,P=0.000).The expression of VEGF was positively correlated with both lymph node metastasis and clinical stage (P=0.009 and P=0.025,respectively).Immunocytochemistry showed that the expression of VEGF was weakly positive and Western blotting indicated a significant reduction in VEGF-siRNA cell protein ***-siRNA cell growth inhibition was assessed by the MTT assay,and the tumor cell proliferation rate was significantly different at 24,48,and 72 h after *** results showed that the VEGF-siRNA group had an apparent aneuploid ***:VEGF,FLT-1 and FLK-1 are associated with colorectal *** silencing of the VEGF gene suppresses proliferation,and induces apoptosis in HCT116 *** results suggest that VEGF may be a new gene therapy target for colorectal cancer.

关键词： Colorectal carcinoma Vascular endothelial cell growth factor Fms-like tyrosine kinase 1 Fetal liver kinase 1 Small interfering RNA

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Satellite-based detection of 16.76 MeV γ-ray from H-bomb D-T fusion

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Nuclear Science and Techniques 2008年第1期19卷 53-57页

作者： CHENG Jinxing WANG Lan OUYANG Xiaoping SHI Jianfang zhang anhui SHEN Chunxia OUYANG Maojie NAN Qinliang Institute of Chemical Defence PLA Beijing 102205 China Northwest Institute of Nuclear Technology Xi＇an 710024 China Academy of Chemical Defence PLA Beijing 102205 China

Based on the high energy γ-ray yield from the H-bomb D-T fusion reaction, it brings forward the idea applying the 16.76 MeV γ-ray to judge whether the H-bomb happens or not, and to deduce the explosion TNT equivalent accurately. The Monte Carlo N-Particle was applied to simulate the high energy γ-ray radiation characteristics reaching the geosynchronous orbit satellite, and the CVD diamond detector suit for the requirements was researched. A series of experiments were carried out to testify the capabilities of the diamond detector. It provides a brand-new approach to satellite-based nuclear explosion detection.

关键词：中子物理金刚石炸弹 D-T融合

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SOME RESULTS ABOUT QUESTIONS PROPOSED BY HALE

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Science China Mathematics 1989年第10期32卷 1164-1173页

作者：张作元 anhui University Hefel 230039 PRC

Consider the geometric characteristic of the asymptotically stable cone of linear differential difference equations x’(t)=A_ox(t)+sum from A_kx(t—γk·r). Two of the open problems proposed by Jack Hale in 1982 have ... 详细信息

关键词： linear differential-difference equations asymptotically stable cone.

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Mad2 and p53 expression profiles in colorectal cancer and its clinical significance

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World Journal of Gastroenterology 2003年第9期9卷 1972-1975页

作者： Gang-Qiang Li Hao Li Hong-Fu zhang Department of Pathology,anhui Medical University,Hefei,230032,anhui Province,China Department of Pathology Anhui Medical University Hefei Anhui Province China. lgqfm@***

AIM: To investigate the expression of tumor suppressor gene p53 and spindle checkpoint gene Mad2, and to demonstrate their expression difference in colorectal cancer and normal mucosa and to evaluate its clinical ***: Westemn blot and immunohistochemistry methods were used to analyze the expression of Mad2 in colorectal cancer and its corresponding normal mucosa. The expression of p53 was detected by immunohistochemistry method in colorectal cancer and its corresponding normal ***: Mad2 was significantly overexpressed in colorectal cancer compared with corresponding normal mucosa (P＜0.001), and it was not related to the differentiation of adenocarcinoma and other dinical factors (P＞0.05).The ratio of Mad2 protein in cancer tissue (C) to that in its normal mucosa tissue (N) was higher than 2, which was more frequently observed in patients with lymph gland metastasis (P＜0.05). p53 protein expression was not observed in normal mucosa. The rate of p53 positive expression in adenocarcinomas was 52.6%. There was a significant difference between adenocarcinomas and normal mucosa(P＜0.001), which was not related to the differentiation degree of adenocarcinoma and other clinical factors (P＞0.05).CONCLUSION: Defect of spindle checkpoint gene Mad2and mutation of p53 gene are involved mainly in colorectal carcinogenesis and C/N＞2 is associated with prognosis of colorectal cancer.

关键词： Mad2 p53 基因表达结肠直肠癌

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THE APPLICATION OF MULTILAYER FEEDFORWARD NETWORK FOR IMAGE SEGMENTATION

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Journal of Electronics(China) 1995年第4期12卷 304-311页

作者：吴小培柴晓冬张德龙 anhui University Hefei 230039

The multilayer feedforward network is used for image segmentation. This paper deals with the procedure of achieving the learning patterns and the method of improving the learning rate. The experiment shows that the im... 详细信息

关键词： Image processing Multilayer feedforward network(MLFN) Image segmentation BP algorithm

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学业成绩与智力和人格特征的相关研究

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心理科学通讯 1990年第5期13卷 28-32,21+66页

作者：张履祥安徽师范大学

本研究表明:1.学业成绩与智力和人格特征相关;2.显示性、慎密性、独立性、敢为性、幻想性、克制性是影响学习的重要人格特征;3.在成绩优良学生中,智力较高与智力较低学生在乐群性、慎密性、幻想性和怀疑性等人格特征上的差异显著,表现... 详细信息

本研究表明:1.学业成绩与智力和人格特征相关;2.显示性、慎密性、独立性、敢为性、幻想性、克制性是影响学习的重要人格特征;3.在成绩优良学生中,智力较高与智力较低学生在乐群性、慎密性、幻想性和怀疑性等人格特征上的差异显著,表现出不同的学习风格。

关键词：非智力心理因素智力发展人格特征学业成绩差学生人格心理特征非智力因素安徽师范大学中小学生智力水平

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Global Mobile Market Situations and China Telecom's Strategy to Enter Mobile Market

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ZTE Communications 2003年第2期1卷 2-7页

作者： zhang Dongbo (anhui Telecom Corporation,China Telecom Group,Hefei 230001,China)

The article briefly introduces the situation of the global mobile communications market,and based on the development status quo of China's mobile communications market,studies the strategy for China Telecom to enter the mobile market,mainly focusing on mo- bile technologies selection,differentiable services,regional strate- gies and price policies.

关键词： CDMA as de Global Mobile Market Situations and China Telecom’s Strategy to Enter Mobile Market been for GSM into of

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南赤爮的两个新变种

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植物研究 1989年第4期9卷 45-46页

作者：张智安微农学院安徽合肥

本文发表了南赤爮的两个新变种:1.大果赤爮Thladiantha nudiflora *** ***,***.2.绵赤爮Thladiantha nudiflora Hemsl,*** ***,***.

关键词：赤爮新变种原变种标本室模式标本绵毛白马寨 Hangzhou anhui lamina

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一种具有多领航者的姿态协同控制算法

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哈尔滨工业大学学报 2013年第3期45卷 1-6页

作者：张安慧孔宪仁张世杰王峰哈尔滨工业大学卫星技术研究所哈尔滨150001

针对姿态角信息拓扑为无向图且角速度信息拓扑为有向图的情况,提出了一种基于相对姿态角和相对角速度信息的包容算法,利用代数图论理论,分析了姿态包容控制对角速度信息拓扑的约束条件.并针对无法获取相对角速度信息的情况,提出一种含... 详细信息

针对姿态角信息拓扑为无向图且角速度信息拓扑为有向图的情况,提出了一种基于相对姿态角和相对角速度信息的包容算法,利用代数图论理论,分析了姿态包容控制对角速度信息拓扑的约束条件.并针对无法获取相对角速度信息的情况,提出一种含角速度阻尼的控制策略,利用李雅普诺夫方法证明了该算法能够实现包容控制.系统仿真验证了所得结论的正确性.

关键词：编队领航-跟随结构姿态协同控制代数图论包容控制

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Asymptotic Behavior of Solutions of Certain Delay Differential Equations with Periodic Coefficients

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数学研究与评论 1985年第2期 74-78页

作者：张书年 anhui University

§1 Introduction In [1], we have discussed the asymptotic behavior and structure of solutions of the linear delay differential equation (1.1) (r>0 is a real constant), and have obtained the following result: If p(t)=c... 详细信息

关键词： Asymptotic Behavior of Solutions of Certain Delay Differential Equations with Periodic Coefficients

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