检索结果-南通市图书馆

A new effective strategy for treating tobacco addiction was developed by researchers from the Chinese Academy of Sciences

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2010年第17期55卷 1841-1842页

作者： zhao baolu

The tobacco addiction epidemic is a major public health problem worldwide. Professor zhao Bao Lu and his group from the State

关键词：中国科学院研究人员烟草治疗国家重点实验室公共卫生问题世界范围认知科学

Structural characteristics of the hydrophobic patch of azurin and its interaction with p53: a site-directed spin labeling study

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2010年第10期53卷 1181-1188页

作者： XU Chao YIN JunJie zhao baolu State Key Laboratory of Brain and Cognitive Science Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate University of the Chinese Academy of Sciences Beijing 100049China Center for Food Safety and Applied Nutrition Food and Drug AdministrationCollege ParkMD 20740USA

Site-directed spin labeling (SDSL) is a powerful tool for monitoring protein structure, dynamics and conformational changes. In this study, the domain-specific properties of azurin and its interaction with p53 were studied using this technique. Mutations of six residues, that are located in the hydrophobic patch of azurin, were prepared and spin labeled. Spectra of the six azurin mutants in solution showed that spin labeled residues 45 and 63 are in a very restricted environment, residues 59 and 65 are in a spacious environment and have free movement, and residues 49 and 51 are located in a relatively closed pocket. Polarity experiments confirmed these results. The changes observed in the spectra of spin labeled azurin upon interaction with p53 indicate that the hydrophobic patch is involved in this interaction. Our results provide valuable insight into the topographic structure of the hydrophobic domain of azurin, as well as direct evidence of its interaction with p53 in solution via the hydrophobic patch. Cytotoxicity studies of azurin mutants showed that residues along the hydrophobic patch are important for its cytotoxicity.

关键词： azurin p53 spin label protein-protein interaction anticancer ESR

Autooxidation Mechanism of Pyrogallol and Its Free Radical Effects Studied by Means of ESR,UV and NMR

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 1999年第2期15卷 54-59页

作者： LIU Shanlin zhao baolu SHI Dongyun XIN Wenjuan Department of Biochemistry Shanghai Medical UniversityShanghai200032 Institute of Biophysics Academia SinicaBeijing100101

ＩｎｔｒｏｄｕｃｔｉｏｎＰｙｒｏｇａｌｏｌ（ｐｙｒｏｇａｌｉｃａｃｉｄ）ｉｓｅａｓｉｌｙａｕｔｏ－ｏｘｉｄｉｚｅｄ，ｆｏｒｍｉｎｇｆｒｅｅｒａｄｉｃａｌｓｕｎｄｅｒａｌｋａｌｉｎｅｃｏｎｄｉｔｉｏｎｓ［１］．Ｗｈｅｎ... 详细信息

ＩｎｔｒｏｄｕｃｔｉｏｎＰｙｒｏｇａｌｏｌ（ｐｙｒｏｇａｌｉｃａｃｉｄ）ｉｓｅａｓｉｌｙａｕｔｏ－ｏｘｉｄｉｚｅｄ，ｆｏｒｍｉｎｇｆｒｅｅｒａｄｉｃａｌｓｕｎｄｅｒａｌｋａｌｉｎｅｃｏｎｄｉｔｉｏｎｓ［１］．Ｗｈｅｎｔｈｅｂｏｄｙｉｓｓｉｃｋ，ｔｈｅ...

关键词： Pyrogallol Free radical ESR NMR UV

L-theanine inhibits nicotine-induced dependence via regulation of the nicotine acetylcholine receptor-dopamine reward pathway

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2012年第12期55卷 1064-1074页

作者： DI XiaoJing YAN JingQi zhao Yan CHANG YanZhong zhao baolu State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Department of Food Sciences and Technology Harbin Institute of Technology at Weihai Weihai 264209 China Institute of Molecular Neurobiology Hebei Normal University Shijiazhuang 050016 China

In this study, the inhibitory effect of L-theanine, an amino acid derivative of tea, on the rewarding effects of nicotine and its underlying mechanisms of action were studied. We found that L-theanine inhibited the rewarding effects of nicotine in a con- ditioned place preference (CPP) model of the mouse and reduced the excitatory status induced by nicotine in SH-SY5Y cells to the same extent as the nicotine receptor inhibitor dihydro-beta-erythroidine (DH E). Further studies using high performance liquid chromatography, western blotting and immunofluorescence staining analyses showed that L-theanine significantly inhibited nicotine-induced tyrosine hydroxylase (TH) expression and dopamine production in the midbrain of mice. L-theanine treatment also reduced the upregulation of the 4, 2 and 7 nicotine acetylcholine receptor (nAChR) subunits induced by nico- tine in mouse brain regions that related to the dopamine reward pathway, thus decreasing the number of cells that could react to nicotine. In addition, L-theanine treatment inhibited nicotine-induced c-Fos expression in the reward circuit related areas of the mouse brain. Knockdown of c-Fos by siRNA inhibited the excitatory status of cells but not the upregulation of TH induced by nicotine in SH-SY5Y cells. Overall, the present study showed that L-theanine reduced the nicotine-induced reward effects via inhibition of the nAChR-dopamine reward pathway. These results may offer new therapeutic strategies for treatment of tobacco addiction.

关键词：乙酰胆碱受体 L-茶氨酸尼古丁多巴胺 SH-SY5Y细胞诱导通路 Western印迹

Copper ions influence the toxicity of β-amyloid(1-42) in a concen-tration-dependent manner in a Caenorhabditis elegans model of Alzheimer’s disease

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2011年第6期54卷 527-534页

作者： LUO YunFeng ZHANG Jie LIU NianQing LUO Yuan zhao baolu State Key Laboratory of Brain & Cognitive Science Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100049China Department of Cell Biology and Neuroscience Rutgers UniversityPiscatawayNJ 08854USA Synchrotron Radiation Laboratory Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Department of Pharmaceutical Sciences University of MarylandBaltimoreMD 21201USA

β-amyloid (Aβ) and copper play important roles in the pathogenesis of Alzheimer’s disease (AD).However,the behavioral correlativity and molecular mechanisms of Aβ and copper toxicity have been investigated less *** the present study,we investigated the interaction and toxicity of Aβ1-42 and copper in the Aβ1-42 transgenic Caenorhabditis elegans worm model *** data show that the paralysis behavior of CL2006 worms significantly deteriorated after exposure to 10-3 mol L-1 copper ***,the paralysis behavior was dramatically attenuated with exposure to 10-4 mol L-1 copper *** exogenous copper treatment also partially changed the homeostatic balance of zinc,manganese,and *** data suggest that the accumulation of reactive oxygen species (ROS) was responsible for the paralysis induced by Aβ and copper in *** ROS generation induced by Aβ and copper appear to be through sod-1,prdx-2,skn-1,hsp-60 and hsp-16.2 genes.

关键词：淀粉样蛋白铜离子浓度依赖性老年痴呆症毒性模型线虫阿尔茨海默氏症

Nitric oxide damages neuronal mitochondria and induces apoptosis in neurons

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2000年第5期45卷 422-426页

作者： Taotao Wei Chang Chen Jingwu Hou baolu zhao Wenjuan Xin Chinese Acad Sci Inst Biophys Beijing 100101 Peoples R China

The cytotoxic effect of nitric oxide on primarily cultured rat cerebellar granule cells was stuatea, and the mechanisms were discussed. The results showed that nitric oxide donor S-nitroso-N-acetyl-penicillamine (SNAP; 500 (imol/L) could induce apoptosis in immature cultures of cerebellar granule cells. Flow cytometry and HPLC analyses revealed that after treatment with SNAP, the mitochondrial transmembrane potential and the cellular ATP content decreased significantly. Nitric oxide scavenger hemoglobin could effectively prevent the neuronal mitochondria from dysfunction and attenuate apoptosis. The results suggested that nitric oxide activated the apoptotic program by inhibiting the activity of mitochondrial respiratory chain and thus decreasing the cellular ATP content.

关键词： cerebellar granule cells apoptosis nitric oxide mitochondria.

在线全文

学校读者我要写书评

暂无评论

基于扎根理论的跨境电商品牌形象塑造研究

丝绸 2024年第5期61卷 16-24页

作者：王保鲁赵思卓北京服装学院服装艺术与工程学院北京100029

为指导跨境电商企业更好地在海外市场塑造品牌形象,提升品牌的国际竞争力,文章基于扎根理论,以跨境电商代表性品牌SHEIN为例,对其在社交平台Instagram上发布的956张宣传图片进行内容分析。借助NVivo 11.0软件,完成201个节点编码,并以此... 详细信息

为指导跨境电商企业更好地在海外市场塑造品牌形象,提升品牌的国际竞争力,文章基于扎根理论,以跨境电商代表性品牌SHEIN为例,对其在社交平台Instagram上发布的956张宣传图片进行内容分析。借助NVivo 11.0软件,完成201个节点编码,并以此生成34个初始范畴、11个主范畴和5个核心范畴。研究发现,基于社交媒体渠道的跨境电商品牌形象塑造,围绕产品特性、消费者形象、品牌价值表达、营销活动、服务互动五个维度展开,其中产品特性与消费者形象传播最为主要,而采用符号化语言的内容输出进一步丰富了品牌跨文化背景下的个性表达。

关键词：跨境电商品牌形象形象塑造社会媒体国际贸易扎根理论

维普期刊数据库博看期刊

Three-dimensional electron spin resonance imaging of endogenous nitric oxide radicals generated in living plants

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2018年第3期4卷 133-142页

作者： Yuanlin Cao Yongsheng Chen Qian Wan Jungai Hu baolu zhao Institute of Biophysics Chinese Academy of Science

Electron spin resonance imaging(ESRI) has been developed to detect the spatial contribution of free radicals in recent years, but all of these studies are used in animal systems and almost of them using exogenous spin probes and only a few to study the endogenous free radicals in vivo. However, there is no report about the endogenous free radical three-dimensional(3 D) EPRI in plant because of low concentration of endogenous free radicals and low resolution of the L-band ESRI. Recently, we have developed the imaging resolution better than 200 lm which is higher 10 times than L-band(about1–3 mm) and the sensitivity is higher about 1000 times than that of L-band ESRI. Using this system and spin trapping technique, we studied spatial contribution of the endogenous nitric oxide(NO) radical generation in wheat leaves and got a clear 3 D ESRI picture and CT(computed tomography) of NO of a wheat leaf. This imaging picture shows a clear spatial distribution of NO free radicals in the leaf. This is the first 3 D ESRI of endogenous NO free radical generated in plant in the world. We have also studied the distributions of NO generation in different plants from different species with different shapes and it is shown that this is a convenient method to study the endogenous free radicals in plants.

关键词： Electron spin resonance (ESR) Electron spin resonance imaging (ESRI) NO free radicals Plant

Antioxidant effects of green tea polyphenols

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2003年第4期48卷 315-319页

作者： zhao baolu Laboratory of Visual Information Processing, Research Centers of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China Laboratory of Visual Information Processing Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

The unprecedented interest in the antioxidant activity of green tea polyphenols (GTP) is due to the appar-ent health benefits of tea drinking and the experimental re-sults with the polyphenols. The results suggest that the gal-loylated catechins show stronger antioxidant effect than that of nongalloylated catechins and the double bond in C ring also plays an important role in this effect. There are synergic effects between different catechins and the antioxidant effect of (+)-epimers is stronger than that of (-)-epimers. The active site to scavenge free radicals mainly locates in p-hydroxyl of phenol in gallic acid and hydroxyl of phenol in the pyrane also shows activity. The large p bond in chromane and benzene ring of catechin is the structure base for their anti-oxidant effects. The prevent effect of GTP against diseases and their redox regulation in cell signal pathway are very important to be studied in the future.

关键词：绿茶茶多酚天然抗氧化剂抗氧化作用生物类黄酮自由基抗衰老