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miR-124在抑郁症中的作用机制

生物化学与生物物理进展 2024年第6期51卷 1316-1326页

作者：薛燕李德珠谢慧莹蒋传邈张俊芳宁波大学医学部浙江省病理生理学技术研究重点实验室宁波315211 宁波大学附属康宁医院宁波315201

抑郁症是世界范围内最常见的精神疾病之一,发病机制复杂,研究仍处于探索阶段。微RNA(miRNA)作为表观遗传机制的重要调控因子,在抑郁症的发生发展中起着重要作用。miR-124是神经系统中表达最丰富的miRNA之一,参与了神经元分化、小胶质细... 详细信息

抑郁症是世界范围内最常见的精神疾病之一,发病机制复杂,研究仍处于探索阶段。微RNA(miRNA)作为表观遗传机制的重要调控因子,在抑郁症的发生发展中起着重要作用。miR-124是神经系统中表达最丰富的miRNA之一,参与了神经元分化、小胶质细胞激活等生物事件。近年研究表明,miR-124在抑郁症患者和动物模型中表达异常,并参与了病理生理机制。然而,miR-124的表达异常情况及其相关机制的研究结果是较复杂的、甚至矛盾的。故本文对此进行梳理,总结了miR-124在抑郁症中的研究进展。

关键词：抑郁症 miR-124 海马糖皮质激素受体神经发生神经炎症

LncRNA在慢性粒细胞白血病中的研究进展

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生命科学 2024年第4期36卷 561-570页

作者：梁荆铁韩兵社张俊芳上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306 上海海洋大学科技部海洋生物科学国际联合研究中心上海201306

慢性粒细胞白血病(chronic myeloid leukemia, CML)是一种由BCR-ABL融合基因驱动的骨髓恶性肿瘤。虽然酪氨酸激酶抑制剂(TKI)可有效抑制BCR-ABL活性,但耐药性仍然是治疗中的主要问题。长链非编码RNA (long non-coding RNA, lncRNA)为长... 详细信息

慢性粒细胞白血病(chronic myeloid leukemia, CML)是一种由BCR-ABL融合基因驱动的骨髓恶性肿瘤。虽然酪氨酸激酶抑制剂(TKI)可有效抑制BCR-ABL活性,但耐药性仍然是治疗中的主要问题。长链非编码RNA (long non-coding RNA, lncRNA)为长度大于200个核苷酸的ncRNA,在肿瘤的发生、发展和耐药中扮演着重要角色。研究发现,lncRNA通过与miRNA互作调控下游信号通路,或影响表观遗传修饰等多种机制影响CML细胞的增殖、凋亡和药物敏感性,是CML的潜在药物靶点。同时,多项研究证明lncRNA是CML有效的诊断、预后生物标志物,如HOX转录反义RNA (HOX transcript antisense RNA,HOTAIR)可应用于CML早期诊断、前列腺癌抗原3 (prostate cancer antigen 3, PCA3)表达水平的改变可作为CML的预后指标。现对CML相关lncRNA的作用机制、与CML临床分期、耐药产生和预后的关系,以及作为诊断、预后生物标志物和药物靶点的研究进展作一综述。

关键词：长链非编码RNA 慢性粒细胞白血病生物标志物药物靶点

人NFE2基因上游增强子lncRNA调控NFE2基因转录和K562细胞增殖

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生物化学与生物物理进展 2024年第1期51卷 190-201页

作者：卢燕飞曲颂雅朱晶晶刘超王建韩兵社张俊芳上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306 临港新片区海洋生物医药科技创新型平台上海201306

目的转录因子NFE2的异常表达在许多骨髓增殖性肿瘤患者中被观察到,然而造成这种异常的转录调控机制尚不明确,本研究旨在探究参与NFE2转录调控的元件和分子机制。方法首先通过公共数据库中Ch IP-seq数据和ATAC-seq数据预测NFE2基因的潜... 详细信息

目的转录因子NFE2的异常表达在许多骨髓增殖性肿瘤患者中被观察到,然而造成这种异常的转录调控机制尚不明确,本研究旨在探究参与NFE2转录调控的元件和分子机制。方法首先通过公共数据库中Ch IP-seq数据和ATAC-seq数据预测NFE2基因的潜在增强子元件,并通过双荧光素酶报告实验进行体外验证。随后,通过PRO-seq和GRO-seq数据结合RACE技术克隆这些增强子RNA转录本,经在线编码潜能预测工具分析认为其为lnc RNA,通过RT-q PCR检测该lnc RNA在不同白血病细胞系中和这些细胞诱导分化前后的表达变化及其亚细胞定位。最后,通过慢病毒系统在K562细胞中过表达和敲降该lnc RNA以探究其功能。结果鉴定出调控NFE2转录的3个增强子元件,分别位于NFE2转录起始位点-3.6k,-6.2k和+6.3k区域,这些元件插入NFE2启动子上游均能增强下游萤火虫荧光素酶的表达。克隆出-3.6k增强子负链方向的转录本,将其鉴定为-3.6k-lnc RNA。本研究发现,该lnc RNA在K562、U937和HL-60这3种白血病细胞系中均有一定程度的表达,且均定位于细胞核内。当该lnc RNA在K562细胞中过表达,NFE2水平随之提高,细胞增殖和细胞迁移能力受到抑制;当其被敲降时,NFE2水平相应降低而K562细胞增殖能力随之升高。结论本文鉴定了调控人NFE2基因转录的3个增强子元件和一条增强子lnc RNA转录本,并验证了该lnc RNA对NFE2转录的正调控作用以及对K562细胞增殖能力具有抑制作用。

关键词： NFE2基因增强子长链非编码RNA 细胞增殖

Non-thermal emission from Vela X and PWN G0.9+0.1

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Chinese Journal of Astronomy and Astrophysics 2009年第4期9卷 449-456页

作者： Wei-Fan Qiao Li zhang jun fang Department of Physics Yunnan University Kunming 650091 China

We study the multi-waveband non-thermal emission from the pulsar wind nebulae （PWNe） Vela X and G0.9＋0.1 in the frame of a time-dependent model describing non-thermal radiation from the PWNe. In such a model, the relativistic wind of particles driven by a central pulsar blows into the ambient medium and creates a termination shock that accelerates the particles to very high energy in a PWN. The non-thermal photons in the PWN are produced both by synchrotron radiation and the inverse Compton process, with electrons coming directly from the pulsar magnetosphere and electrons being accelerated at the termination shock. We apply this model to reproduce the observed multi-waveband photon spectra of Vela X and the G0.9＋0.1, both of which have been detected emitting very high energy photons. Our results indicate that TeV photons are produced by the inverse Compton scattering of the high-energy electrons in the infrared photon field in both Vela X and PWN G0.9＋0.1. The TeV photons from these two PWNe may have leptonic origins.

关键词： ISM individual objects Vela X, G0.9 + 0.1-gamma-rays theory

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载体材料对汉防己甲素固体分散体性质的影响

中国新药杂志 2024年第7期33卷 714-721页

作者：冯唐德欧丽泉赵国巍敖腾张俊芳蔡萍江西中医药大学现代中药制剂教育部重点实验室南昌330004

目的:研究载体材料对汉防己甲素(TET)-固体分散体(SD)性质的影响。方法:分别以聚乙烯吡咯烷酮K30(PVPK30)、羟丙基甲基纤维素55(HPMC55)和Soluplus^(■)为载体材料,按TET-载体材料1∶4的质量比,采用喷雾干燥法制备了TET-SD,并对TET-SD... 详细信息

目的:研究载体材料对汉防己甲素(TET)-固体分散体(SD)性质的影响。方法:分别以聚乙烯吡咯烷酮K30(PVPK30)、羟丙基甲基纤维素55(HPMC55)和Soluplus^(■)为载体材料,按TET-载体材料1∶4的质量比,采用喷雾干燥法制备了TET-SD,并对TET-SD的理化性质及溶出行为进行研究。结果:理化性质研究结果表明,3种TET-SD均为无定形态,药物和载体材料之间存在分子间相互作用,水接触角减小,粉体均为表面光滑的球形,粉体粒径显著减小。统计分析结果表明,TET-PVPK30-SD和TET-HPMC55-SD中TET的溶出行为与相应的物理混合物(PM)相比没有显著差异,TET-Soluplus^(■)-SD中TET的溶出行为与TET-Soluplus^(■)-PM相比显著改善。结论:3种无定形状态TET-SD对TET溶出行为的改善存在显著差异,可能是由于TET-PVPK30-SD的表面自由能极性成分含量和铺展系数与相应的TET-PVPK30-PM相近,使TET-PVPK30-SD中TET的溶出行为与TET-PVPK30-PM中TET的溶出行为相比没有显著差异;TET-HPMC55-SD的铺展系数较小,使TET在TET-HPMC55-SD中分散不均匀,可能存在相分离,影响了TET的溶出。

关键词：汉防己甲素固体分散体溶出表面自由能铺展系数

Formation and thermal stability of amorphous Ni-Mo-P alloys

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中国有色金属学会会刊：英文版 2004年第z1期14卷 147-151页

作者： zhang Yi MA jun fang Yong-kui DUAN Ji-guo School of Municipal and Environmental Engineering Harbin Institute of TechnologyHarbin 150090 China College of Chemistry and Chemical Engineering Daqing Petroleum Institute Daqing 163318 China College of Chemistry and Chemical Engineering Daqing Petroleum Institute Daqing 163318 China School of Municipal and Environmental Engineering Harbin Institute of TechnologyHarbin 150090 China School of Municipal and Environmental Engineering Harbin Institute of TechnologyHarbin 150090 China

The experimental researches on the chemical deposition of Ni-Mo-P amorphous alloys were carried out by adding Na2 MoO4 into acidic solutions. The optimum technology conditions were obtained by orthogonal design experiments. The structures and the relationship between compositions and their thermal stability were studied by energy spectrum (EC), scanning electron micrograph and X-ray diffraction spectrum. Compared with Ni-P amorphous alloys, the Ni-Mo-P amorphous alloys have high crystallization temperature and thermal stability, and the hardness reaches its peak when the annealing temperature is 500 ℃. With the increase of the heat treatment temperature, the surface morphology of the alloys changes.

关键词： chemical deposition Ni-Mo-P alloy amorphous phase crystallization

Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity

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Chinese Journal of Natural Medicines 2020年第12期18卷 934-940页

作者： KANG Li-Hua zhang Guo-Wei zhang jun-fang QIN Bai GUAN Huai-Jin Eye Institute Affiliated Hospital of Nantong UniversityNantong 226000China

A contributory role of oxidative stress and protection by antioxidant nutrients have been suspected in cataract *** acid A(GAA),an effective lanostane triterpene,is widely reported as an *** aim of this study is to investigate the potential effects of GAA on cataract *** lens epithelial cells(LECs)were exposed to UVB radiation for different periods,cell viability,apoptosis-related protein levels,malondialdehyde(MDA)and superoxide dismutase(SOD)activities were *** found that cell viability,the Bcl-2/Bax ratio and SOD activity were increased,while Cleaved caspase-3 levels and MDA activity were decreased compared with those in UVB-impaired LECs after GAA ***,GAA activated PI3 K/AKT in UVB-impaired LECs and effectively delayed the occurrence of lens opacity in *** conclusion,these findings demonstrated that GAA exhibited protective functions in SRA01/04 cells and rat lenses against UVB-evoked impairment through elevating cell viability and antioxidant activity,inhibiting cell apoptosis,activating the PI3 K/AKT pathway and delaying lens opacity.

关键词： Ganoderic acid A Cataract Lens epithelial cells Cell vitality Apoptosis

Spatially explicit reconstruction of cropland cover in Europe from AD 1800 to 2000

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Science China Earth Sciences 2024年第9期67卷 2953-2971页

作者： jun LI Yu YE Xiuqi fang Diyang zhang Zhilei WU Faculty of Geographical Science Beijing Normal UniversityBeijing 100875China Key Laboratory of Environmental Change and Natural Disaster Ministry of EducationBeijing Normal UniversityBeijing 100875China College of Geographical Science Qinghai Normal UniversityXining 810016China

One of the challenges in global change research is the significant uncertainty in global historical land use and land cover(LUCC)datasets,which are widely used as foundational *** addition to the regional cropland area reconstructions,improving the grid allocation method is another feasible way to raise the reliability of historical LUCC *** this study,an integrated reconstruction of the national cropland areas over the past 200 years was developed for 36 European *** that,the allocation algorithm was built using physiogeographic variables and historical city sites for accounting for land suitability and cultivation preferences,***,cropland data in Europe with a spatial resolution of 5′×5′at five time sections from AD 1800 to 2000 were generated using the optimal allocation algorithm in accordance with the stages of the regional *** results were as follows:(1)The dominant factors governing the distribution of croplands in Europe vary at different agricultural stages,but the results can be merged *** suitability was more optimal for allocation during the modern agricultural stage(AD 1950 and 2000);the priority index combined with land suitability and cultivation preference was more reasonable for allocation during the traditional agricultural stage(AD 1800).The average of the allocations by priority index and the land suitability could be adopted as the allocation results during the transitional stage(AD 1850 and 1900)because the grids for absolute differences within±10 and±20 percentage points between the results obtained from the above two allocations were above 80% and 95%,respectively,which means the two allocation results could be merged.(2)Over the past 200 years,the total cropland area in Europe first increased to a peak in AD 1900 and then ***,the centre of the higher cropland fraction shifted from the western part of Europe in AD 1800 to the eastern part of the continent after AD 1950.(3)

关键词： Land use and land cover change Historical cropland Gridding allocation Europe Past 200 years

Expression and Emmprin Regulated Function of Aquaporinl in SMMC7221 Cells

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Chemical Research in Chinese Universities 2010年第4期26卷 578-582页

作者： LI Jiang zhang Ai-li TONG Qian zhang jun-fang LI Hai-jing ZHAO Bing MA Tong-hui LI Xiao-men School of Life Sciences Northeast Normal University Changchun 130024 P. R. China Dental Hospital First Hospital Jilin University Changehun 130021 P. R. China Department of Cardiology First Hospital Jilin University Changehun 130021 P. R. China

Herein lie the crosstalk and regulation between AQP1 and Emmprin in SMMC7221 cells by means of siRNA technology and deglycosylation method. Firstly, HAQP1, rather than hAQP3, was selectively upregulated in SMMC7221 cells by FBS, flollowed by the upregulated expression of Emmprin. Emmprin gene silencing caused a remarkable change in the expression ofAQP1 gene, just like its downstream gene, MMP9, meanwhile the water permeability and cell migration were also descended prominently. Furthermore, when treated with tunicamycin, Emmprin was deglycosylated, which made the expression of AQP 1 significantly declined, followed by remarkably decreased cell membrane water permeability and cell migration. Taken together, all the data indicates the expression level and the modification of Emmprin by glycosylation are the key factors in regulating the expression of AQP1.

关键词： Aquaporinl Emmprin Cell migration