检索结果-南通市图书馆

Transcriptomic analysis identifies differentially expressed genes(DEGs) associated with bolting and flowering in Saposhnikovia divaricata

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2018年第6期16卷 446-455页

作者： LIU Shuang-Li WANG Xiao-Hui GAO Yu-Gang ZHAO Yan zhang ai-hua XU Yong-hua zhang Lian-Xue College of Chinese Medicinal Materials Jilin Agricultural University Research Center of Agricultural Environment and Resources Jilin Academy of Agricultural Sciences

Saposhnikovia divaricata is a valuable Chinese medicinal herb; the transformation from vegetative growth to reproductive growth may lead to the decrease of its pharmacological activities. Therefore, the study of bolting and flowering for Saposhnikovia divaricata is warranted. The present study aimed to reveal differentially expressed genes(DEGs) and regularity of expression during the bolting and flowering process, and the results of this study might provide a theoretical foundation for the suppression of early bolting for future research and practical application. Three sample groups, early flowering, flower bud differentiation, and late flowering(groups A, B, and C, respectively) were selected. Transcriptomic analysis identified 67, 010 annotated unigenes, among which 50, 165 were differentially expressed including 16, 108 in A vs B, and 17, 459 in B vs C, respectively. Gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway functional classification analysis were performed on these differentially expressed genes, and five important pathways were significantly impacted(P ≤ 0.01): plant circadian rhythm, other glycan degradation, oxidative phosphorylation, plant hormone signal transduction, and starch and sucrose metabolism. Plant hormone signal transduction might play an important role in the bolting and flowering process. The differentially expressed indole-3-acetic acid(IAA) gene showed significant down-regulation during bolting and flowering, while the transport inhibitor response 1(TIR1) gene showed no significant change during the bolting process. The expression of flowering related genes FLC, LYF, and AP1 also showed a greater difference at different development stages. In conclusion, we speculate that the decrease in auxin concentration is not caused by the degrading effect of TIR1 but by an alternative mechanism.

关键词： Saposhnikovia divaricata Transcriptornics Auxin Bolting Hormone signalling

Study of Differential Serum Metabolites in Patients with Adenomatous Polyps of Colon and Yang-Deficiency Constitution Based on Ultra-Performance Liquid Chromatography-Mass Spectrometry

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2022年第5期28卷 403-409页

作者： DU Wen-zhang zhang ai-hua REN Jun-ling LYU Kun TUO Lu-yao XU Wei Department of Integrated Traditional Chinese and Western Medicine First Affiliated Hospital of Harbin Medical UniversityHarbin(150001)China Metabolomics Laboratory Heilongjiang University of Chinese MedicineHarbin(150001)China

Objective:To study the differences between the serum metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those without colon polyps and with balanced constitution,and look for biomarkers that can be used to distinguish between the two ***:General patient information was gathered,and Chinese medicine constitutions were collected in 940 patients who underwent electronic colonoscopy.A total of 119 patients with adenomatous polyps of the colon and yang-deficiency constitution were included in the experimental group,and 150 patients without colon polyps and with balanced constitution were included in the control *** analysis was performed on the fasting venous blood obtained from each patient in both *** component analysis and orthogonal partial least squares discriminant analysis were performed on the detection results,potential biomarkers were screened,metabolic pathway changes were determined,and the metabolic processes involved were ***:A total of 59 differential biomarkers between the experimental group and the control group were *** differential metabolites were found mainly in the glycerophospholipid metabolism pathway,and the bile acid 3-oxo-4,6-choladienoic acid was the biomarker that distinguished the experimental group from the control ***:With the help of metabolomics analysis,the differential metabolites in patients with adenomatous polyps of the colon and yang-deficiency constitution and those in patients without colon polyps and with balanced constitution could be *** biomarker 3-oxo-4,6-choladienoic acid may have potential diagnostic value in patients with adenomatous polyp of the colon and yang-deficiency constitution.(Trial Registration ***02986308)

关键词： ultra-performance liquid chromatography-mass spectrometry adenomatous polyps yang-deficiency constitution Chinese medicine metabolomics biomarkers

Characterization and Comparison of Ochratoxin A-Ovalbumin(OTA-OVA) Conjugation by Three Methods

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第5期13卷 1130-1136页

作者： WANG Xi-chun BAO Ming WU Jin-jie LUO Ying MA Liang-you WANG Ying zhang ai-hua HE Cheng-hua zhang Hai-bin College of Animal Science and Technology Anhui Agricultural University Aquatic Technology Promotion Station of Anhui Province College of Veterinary Medicine Nanjing Agricultural University

In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten （ochratoxin A, OTA） was coupled to ovalbumin （OVA） by an active ester reaction. To develop a technique for detecting the conjugation, the hapten-protein conjugate （OTA-OVA） was characterized thoroughly by immunoarray technology, ultraviolet （UV） spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF-MS）, respectively. The molecular weight of OTA-OVA was 50 350.141 Da, and the molecular weight of OVA was 44 887.506 Da, which were determined by MALDI-TOF-MS, respectively. In OTA-OVA, the molecular coupling ratio was 13：1 by MALDI-TOF-MS while the molecular coupling ratio was 10：1 by UV. In this experiment, UV and MALDI-TOF-MS were selected as the efficient methods to evaluate the cross-linking effect and calculate the molecular coupling ratio.

关键词： ochratoxin A conjugated antigen cartier protein coupling ratio

Determination of the Refractive Index Profile of a Polymer Waveguide by Photobleaching

在线全文

学校读者我要写书评

暂无评论

Chinese Physics Letters 2000年第6期17卷 433-434页

作者： SHAN Ji-Xiang SHEN Qi-Shun zhang ai-hua CAO Zhuang-Qi CHEN Ying-Li Department of Applied Physics Jiao Tong UniversityShanghai 200030

The shrinkage effect of polymer films,which was demonstrated in our photobleaching experiment,can be fitted approximately by an exponentially decaying *** taking the shrinkage effect into account,the index profile of the polymer waveguide was determined to be a Fermi function with the help of the m-line method and the exact transfer matrix theory.

关键词： function waveguide Waveguide

P阶Feigenbaum函数方程的单峰连续偶解

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

南京邮电大学学报（自然科学版） 2008年第5期28卷 57-59页

作者：张爱华南京邮电大学数理学院江苏南京210003 南京师范大学数学与计算机科学学院江苏南京210097

为了更好的解释Feigenbaum现象,研究了第一类Feigenbaum函数方程的单峰连续偶解和第二类Feigenbaum函数方程的单谷连续解之间的关系,利用构造性的方法证明了以下结论:P阶Feigenbaum函数方程存在单峰连续的偶解,本文推广了以前相关的结果。

关键词： Feigenbaum函数方程单谷连续解单峰连续偶解

中国特色哲学理论体系构建态势管窥——以《社会科学文摘》(2016—2020)相关转载数据为基础

在线全文

学校读者我要写书评

暂无评论

云南社会科学 2021年第6期 32-38,185,186页

作者：张爱华上海社会科学院社会科学杂志社上海社会科学院《社会科学文摘》编辑部

在国内外环境发生深刻变化的时代背景下,构建中国当代的哲学理论体系——这一历史性的重大命题进一步凸显出来,成为近些年中国哲学界最重要的理论热点。梳理《社会科学文摘》(2016—2020)与中国特色的哲学理论体系构建相关的创新性成果... 详细信息

在国内外环境发生深刻变化的时代背景下,构建中国当代的哲学理论体系——这一历史性的重大命题进一步凸显出来,成为近些年中国哲学界最重要的理论热点。梳理《社会科学文摘》(2016—2020)与中国特色的哲学理论体系构建相关的创新性成果,可以深入到中国哲学、西方哲学和马克思主义哲学内部,追踪其动态演变,可以触摸到“‘中国性’的迅速成长”“中西马融合深度不断加深”“新的理论框架和倡议不断出现”等理论发展的整体态势。面向未来,中国特色的哲学理论体系,可能是中西马哲学混合而成的某种松散的开放的哲学综合体,也许还可能是高度融通了中西马哲学的一体化哲学。

关键词：中国特色的哲学理论体系构建态势《社会科学文摘》

农村中年女性的温情策略与家庭关系期待——对河北上村隔代照顾实践的研究

在线全文

学校读者我要写书评

暂无评论

妇女研究论丛 2015年第5期 19-28页

作者：张爱华南京农业大学社会学系江苏南京210095

文章采取民族志的研究方法,对河北上村的隔代照顾进行研究发现,农村中年女性通过"反思性生涯"发展新型的代际策略。与传统婆婆在代际支持上的"缺席"不同,中年女性积极营造与儿媳妇的代际关系,她们通过出让工作权利,诉诸亲密关系,力图"生... 详细信息

文章采取民族志的研究方法,对河北上村的隔代照顾进行研究发现,农村中年女性通过"反思性生涯"发展新型的代际策略。与传统婆婆在代际支持上的"缺席"不同,中年女性积极营造与儿媳妇的代际关系,她们通过出让工作权利,诉诸亲密关系,力图"生产"融洽的代际关系与和睦的家庭伦理,进而"激发"子辈的感激、依恋乃至对其未来养老的承诺。但是,在父权制与市场经济的双重作用下,中年女性的权利在代际关系重构的图景中隐而不见,她们不得不面临权力丧失与主体性消融的困境。

关键词：代际关系中年女性反思性生涯策略困境

在线全文

学校读者我要写书评

暂无评论

“进村找庙”之外:水利社会史研究的勃兴

史林 2008年第5期 166-177,188页

作者：张爱华华东师范大学历史系

上世纪90年代中期以来,特别是进入新世纪,水利社会史研究在国内蓬勃兴起,由于参与学者学术背景不同,治学方法相异,因而对水利社会的研究形成了多样化研究路径,为进一步拓展区域社会史研究视野提供了新平台。

关键词：水利社会史水利社会

基于特征双重加权支持向量机的放大器性能综合评价研究

在线全文

学校读者我要写书评

暂无评论

电路与系统学报 2012年第3期17卷 77-82页

作者：张爱华渤海大学工学院辽宁锦州121000

本文针对于现有放大器评价方法的人为因素过大或是成本过高等状况,而提出了基于特征双重加权支持向量机的放大器性能综合评价方法。确定了放大器性能的综合评价系统结构,以高校模拟电子技术实验为依托,采用近一年内由幅频特性测试仪测... 详细信息

本文针对于现有放大器评价方法的人为因素过大或是成本过高等状况,而提出了基于特征双重加权支持向量机的放大器性能综合评价方法。确定了放大器性能的综合评价系统结构,以高校模拟电子技术实验为依托,采用近一年内由幅频特性测试仪测评所得相关放大器的四项指标构建训练集,然后进行特征双重加权支持向量机的四分类评价。实验表明,该方法优于传统的方法,提高了参数测量的精度,可推广于电子产品的综合性能评价中去。

关键词：特征双重加权支持向量机放大器性能综合评价分类器