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夏玉米对花期高温逆境的响应与耐热性分析

玉米科学 2024年第5期32卷 40-49,59页

作者：张志方张守林徐国举王良发刘桂珍鹤壁市农业科学院河南鹤壁458030 河南省种业发展中心郑州450008

以浚单996(XD996)、郑单958(ZD958)、先玉335(XY335)为试验材料,采用温控试验,设自然温度(CK,29~32℃)、高温(HT,30、35、38、40℃)处理,研究花期不同程度高温对花粉活力和花丝活力的影响。采用高温胁迫试验,研究花期高温对受精结实、... 详细信息

以浚单996(XD996)、郑单958(ZD958)、先玉335(XY335)为试验材料,采用温控试验,设自然温度(CK,29~32℃)、高温(HT,30、35、38、40℃)处理,研究花期不同程度高温对花粉活力和花丝活力的影响。采用高温胁迫试验,研究花期高温对受精结实、果穗发育、产量及构成因素的影响。结果表明,随着温度升高,各品种高活力花粉呈降低趋势,低活力花粉呈先增后降趋势,无活力花粉呈增加趋势,花丝活力呈降低趋势。模型拟合分析结果表明,以50%为阈值,XD996、ZD958、XY335的花粉活力耐高温临界温度分别为37.0、35.4、33.2℃,花丝活力耐高温临界温度分别为37.4、35.6、33.7℃。高温处理条件下,XD996、ZD958、XY335的受精率分别较CK降低8.8%、12.9%、20.1%,结实率分别较CK降低9.9%、18.4%、36.4%,结实受精比分别较CK降低1.3%、6.2%、20.3%。耐热系数聚类分析结果表明,穗长、穗行数、行粒数、产量为主要耐热鉴定指标,穗粗、秃尖长、百粒重为次要耐热鉴定指标。

关键词：玉米花期高温逆境受精结实耐热系数

SIMULATION OF THERMAL TRANSPORT IN AQUIFER:A GWHP SYSTEM IN CHENGDU,CHINA

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Journal of Hydrodynamics 2009年第5期21卷 647-657页

作者： ZHOU Yan-zhang ZHOU zhi-fang Institute of Groundwater Science and Engineering Hohai University Nanjing 210098 China

With an established convection-dispersion model for the thermal transport in aquifer, the thermal transport processes in an unconfined aquifer of a Groundwater Heat Pump （GWHP） system in Chengdu, China, are simulated with double-well intervals and cooling-load design fluctuations in summer running period under special groundwater flow and heat source conditions, and the stage-characteristics of the thermal transport in the aquifer are investigated in the running cycle （1 year） numerically. The results show that the thermal transport in the aquifer is closely related to the distance between pumping and injecting wells and the cooling-load design fluctuations, especially, to the cycling water volume. The thermal transport in the aquifer sees different characteristics in the two states in the pumping-recharging stages in summer/winter and the storing stages in spring/autumn. With the hydro-geologic theory and the numerical model, the effect of the groundwater-flow on the thermal transport in the unconfined aquifer is discussed.

关键词： Groundwater Heat Pump (GWHP) thermal transport in aquifer numerical simulation groundwater flow

德昂酸茶水提物对高脂饮食小鼠肠道病理及脂肪代谢相关基因表达的影响

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粮食与油脂 2024年第6期37卷 148-152页

作者：杨卫星侯艳张智芳肖蓉杨莉云南农业大学食品科学技术学院云南昆明650201 云南农业大学龙润普洱茶学院云南昆明650201 滇西应用技术大学普洱茶学院云南普洱665000 普洱学院生物与化学学院云南普洱665000

通过灌胃不同剂量的德昂酸茶水提物,研究德昂酸茶对高脂饮食诱导肥胖小鼠的生理指标、肠道病理及脂肪代谢等相关基因的影响。结果表明:与高脂对照组相比,德昂酸茶可显著降低高脂饮食小鼠的体质量、腹脂比(P<0.05),对高脂饮食造成的小鼠... 详细信息

通过灌胃不同剂量的德昂酸茶水提物,研究德昂酸茶对高脂饮食诱导肥胖小鼠的生理指标、肠道病理及脂肪代谢等相关基因的影响。结果表明:与高脂对照组相比,德昂酸茶可显著降低高脂饮食小鼠的体质量、腹脂比(P<0.05),对高脂饮食造成的小鼠小肠组织肠黏膜损伤具有一定的改善和保护作用;同时德昂酸茶中剂量组能显著上调高脂饮食小鼠肝脏组织、脂肪组织中PPARα、AOX、CPT-1的基因表达水平,并有效下调肝脏组织中脂肪酸合成酶的基因表达水平。

关键词：德昂酸茶高脂饮食脂肪代谢基因表达

Synthesis, Crystal Structure and Anti-breast Cancer Activity of ((3S,5R)-5-((1H-1,2,4-triazol-1-yl)methyl)-5-(2,4-difluorophenyl)tetrahydrofuran-3-yl)methyl 4-Methylbenzenesulfonate

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Chinese Journal of Structural Chemistry 2019年第8期38卷 1337-1343页

作者： HAN Hao zhang zhi-fang zhang Jun-Fei zhang Bin Tongliao Inner Mongolia Autonomous RegionChina Nursing Department Tongliao City HospitalTongliaoInner Mongolia Autonomous RegionChina The First Affiliated Hospital of Inner Mongolia University for theNationlities TongliaoInner Mongolia Autonomous RegionChina

The new heterocycle compound ((3S,5R)-5-((1H-1,2,4-triazol-1-yl)methyl)-5-(2,4-difluorophenyl)tetrahydrofuran-3-yl)methyl 4-methylbenzenesulfonate (1), designed using((3R,5S)-5-(2,4-difluorophenyl)-5-iodotetrahydrofuran-3-yl)methyl isobutyrate(2) as the start material, was successfully obtained via multiple synthesis route and finally characterized by IR, ~1H NMR, and single-crystal X-ray crystallography. In addition, the in vitro anticancer activities of the newly synthesized complex 1 have been emulated against three human breast cancer cell lines BT474, MCF7 and MB.

关键词： heterocycles X-ray crystallography anticancer activity

Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants

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Agricultural Sciences in China 2009年第6期8卷 643-651页

作者： LI Yi-nü SUN Bing-yao SU Ning MENG Xiang-xun zhang zhi-fang SHEN Gui-fang Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China College of Life Sciences Suzhou University Suzhou 215123 P.R.China Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

In contrast to the situation of random integration of foreign genes in nuclear transformation, the introduction of genes via chloroplast genetic engineering is characterized by site-specific pattern via homologous recombination. To establish an expression system for alien genes in rice chloroplast, the intergenic region of ndhF and trnL was selected as target for sitespecific integration of PPT-resistant bar gene in this study. Two DNA fragments suitable for homologous recombination were cloned from rice chloroplast genome DNA using PCR technique, and the chloroplast-specific expression vector pRB was constructed by fusing a modified 16S rRNA gene promoter to bar gene together with terminator ofpsbA gene 3 sequence. Chloroplast transformation was carried out by biolistic bombardment of sterile rice calli with the pRB construct. Subsequently, the regenerated plantlets and seeds of progeny arising from reciprocal cross to the wild-type lines were obtained. Molecular analysis suggested that the bar gene has been integrated into rice chloroplast genome. Genetic analysis revealed that bar gene could be transmitted and expressed normally in chloroplast genome. Thus, the bar gene conferred not only selection pressure for the transformation of rice chloroplast genome, but PPT-resistant trait for rice plants as well. It is suggested that an efficient gene expression system in the rice chloroplast has been established by chloroplast transformation technique.

关键词： Oryza sativa L. chloroplast transformation bar gene homologous fragments

Enrichment and reconfiguration of titanium-bearing phase in vortex smelting reduction process of vanadium-titanium magnetite

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Journal of Iron and Steel Research International 2024年第7期31卷 1582-1589页

作者： Yong-chao Han zhi-he Dou Rui zhang Ting-an zhang Shuai fang Key Laboratory for Ecological Metallurgy of Multimetallic Ores(Ministry of Education) Shenyang 110819LiaoningChina School of Metallurgy Northeastern UniversityShenyang 110819LiaoningChina

To comprehensively utilize the low-iron high-vanadium-titanium magnetite,a new method of vortex smelting reduction of vanadium-titanium magnetite was proposed,and the enrichment and reconstitution regularity of Ti-bearing phases in the slag was investigated through X-ray fluorescence spectrometry,X-ray photoelectron spectroscopy,X-ray diffraction analysis,and optical *** phase diagram revealed that the preferential crystallization of MgTi_(2)O_(5) can be achieved by adjusting the CaO,MgO,and TiO_(2) contents of *** predominant Ti-bearing phases in the slag obtained from the reduction process are MgxTi_(3_x)O_(5)(0≤x≤1)and CaTiO_(3).FeTiO_(3) is present at carbon-iron ratio(CR)=1.3,while MgTi_(2)O4 and TiC are formed at CR=*** enrichment of TiO_(2) in the slag increases first and then decreases as the CR increases,and at CR=1.1,the enrichment of TiO_(2) in the slag reaches 51.3 wt.%.Additionally,the concentrations of MgxTi_(3_x)O_(5)(0≤x≤1)and CaTiO_(3) in the slag,along with the grain width of MgxTi_(3_x)O_(5)(0≤x≤1),decrease with the increase in CR.

关键词： Vanadium-titanium magnetite Vortex smelting reduction Phase reconfiguration TiO_(2)enrichment

中美李斯特菌病流行特点和食品溯源及对我国的启示

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中国人兽共患病学报 2024年第7期40卷 636-643页

作者：张智芳刘雪杰吴生根李曲文陈伟伟邓艳琴福建省疾病预防控制中心/福建省人兽共患病重点实验室福州350012

目的分析比较美国和中国李斯特菌病(Listeriosis)的流行特点,为我国李斯特菌病的防控提供参考依据。方法对美国2009-2021年李斯特菌病监测资料进行描述性统计分析,并根据美国疫情监测数据进行食品归因估计。国内数据来源:搜集中国知网... 详细信息

目的分析比较美国和中国李斯特菌病(Listeriosis)的流行特点,为我国李斯特菌病的防控提供参考依据。方法对美国2009-2021年李斯特菌病监测资料进行描述性统计分析,并根据美国疫情监测数据进行食品归因估计。国内数据来源:搜集中国知网、万方医学、维普等数据库报道的李斯特菌病病例,并对散发病例的流行病学特征进行描述性统计,同时对食品溯源成功的案例进行回顾性分析。结果美国2009—2021年共报告1037例李斯特菌病病例,住院902人,死亡165人。因单增李斯特菌(Listeria monocytogenes,***)污染食品引起的发病高峰在7月份,其发病人数、住院人数和死亡人数分别占总数的18.4%(191/1037)、20.5%(185/902)和22.4%(37/165)。大多数李斯特菌病的暴发被归因于3类食物:乳制品、行作蔬菜和水果,归因百分比的范围在73.8%~89.6%。中国李斯特菌病的总体发病数量不高,2009-2021年共报告619例,有详细发病年份记载的仅177例,其中2018年最多为26例。有详细发病月份报道的病例有220例,其中4月份发病人数最多为30例。中国的李斯特菌病病例资料不完善,且多散在发生,仅7例病例成功追溯到原因食品。结论美国对Lm的监测资料比较完善,食源性暴发病例较多,乳制品、行作蔬菜和水果是最容易引起疾病暴发的原因食品,中国仅有散发病例报道。中国应逐步完善多部门协作机制,实现数据共享并加深对数据的挖掘,加快推进食源性致病微生物的检测技术的进步,防止Lm污染食品引起疾病的暴发,保障食品安全和人民健康。

关键词：食源性疾病单增李斯特菌归因估计全基因组测序李斯特菌病食品召回

Thermally Conductive,Healable Glass Fiber Cloth Reinforced Polymer Composite based onβ-Hydroxyester Bonds Crosslinked Epoxy with Improved Heat Resistance

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Chinese Journal of Polymer Science 2024年第5期42卷 643-654,I0009页

作者： fang Chen Xiao-Yan Pang Ze-Ping zhang Min-zhi Rong Ming-Qiu zhang Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education GD HPPC LabIGCMESchool of ChemistrySun Yat-sen UniversityGuangzhou510275China Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Jieyang515200China

To simultaneously endow thermal conductivity,high glass transition temperature(Tg)and healing capability to glass fiber/epoxy(GFREP)composite,dynamic crosslinked epoxy resin bearing reversibleβ-hydroxyl ester bonds was reinforced with boron nitride nanosheets modified glass fiber cloth(GFC@BNNSs).The in-plane heat conduction paths were constructed by electrostatic self-assembly of polyacrylic acid treated GFC and polyethyleneimine decorated ***,the GFC@BNNSs were impregnated with the mixture of lower concentration(3-glycidyloxypropyl)trimethoxysilane grafted BN micron sheets,3,4-epoxycyclohexylmethyl 3,4-epoxycyclohexanecarboxylate and hexahydro-4-methylphthalic anhydride,which accounted for establishing the through-plane heat transport pathways and avoiding serious deterioration of mechanical *** resultant GFREP composite containing less boron nitride particles(17.6 wt%)exhibited superior in-plane(3.29 W·m^(-1)·K^(-1))and through-plane(1.16 W·m^(-1)·K^(-1))thermal conductivities,as well as high Tg of 204℃(Tg of the unfilled epoxy=177℃).The reversible transesterification reaction enabled closure of interlaminar cracks within the composite,achieving decent healing efficiencies estimated by means of tensile strength(71.2%),electrical breakdown strength(83.6%)and thermal conductivity(69.1%).The present work overcame the disadvantages of conventional thermally conductive composites,and provided an efficient approach to prolong the life span of thermally conductive GFREP laminate for high-temperature resistant integrated circuit application.

关键词： Thermally conductive composites Boron nitride High-temperature resistance β-Hydroxyl ester bond Healing

基于Cox健康行为互动模式的护理措施在宫颈癌患者护理中的应用

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海南医学 2024年第14期35卷 2098-2102页

作者：张雪英张智芳段小俊田会利杨君新乡医学院第一附属医院妇科二病区河南卫辉453100 新乡医学院第一附属医院妇科一病区河南卫辉453100

目的探讨基于Cox健康行为互动模式的护理措施在宫颈癌患者护理中的应用效果。方法选择2022年3月至2023年3月新乡医学院第一附属医院收治的96例宫颈癌术后患者作为研究对象,按随机数表法将患者分为对照组和观察组各48例。对照组患者术后... 详细信息

目的探讨基于Cox健康行为互动模式的护理措施在宫颈癌患者护理中的应用效果。方法选择2022年3月至2023年3月新乡医学院第一附属医院收治的96例宫颈癌术后患者作为研究对象,按随机数表法将患者分为对照组和观察组各48例。对照组患者术后采用常规护理,观察组患者在对照组护理的基础上给予基于Cox健康行为互动模式的护理措施,两组患者均接受护理至出院,出院后均随访3周。护理结束后出院时,比较两组患者的术后恢复情况(手术切口愈合时间、留置导尿管时间、住院时间)以及护理前、护理后T淋巴细胞亚群中CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平;随访3周回院复查时,比较两组患者的生活质量量表(SF-36)评分;护理结束后出院时,比较两组患者对护理工作的满意度。结果观察组患者的手术切口愈合时间、住院时间、留置导尿管时间分别为(7.60±1.39)d、(1.50±0.24)周、(17.78±2.11)d,明显短于对照组的(10.55±1.51)d、(2.54±0.30)周、(20.10±2.32)d,差异均有统计学意义(P<0.05);观察组患者护理后的CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)水平分别为(70.64±11.67)%、(39.91±5.23)%、1.50±0.27,明显高于对照组的(66.25±11.32)%、(35.21±5.08)%、1.24±0.18,差异均有统计学意义(P<0.05);随访3周回院复查时,观察组患者的家庭生活、精神活力、社交能力、心理承受评分分别为(36.14±1.58)分、(36.02±1.57)分、(37.10±2.55)分、(36.26±2.31)分,明显高于对照组的(32.31±1.62)分、(30.15±1.63)分、(32.19±2.40)分、(33.11±2.14)分,差异均有统计学意义(P<0.05);观察组患者对护理工作的满意度为95.83%,明显高于对照组的83.33%,差异有统计学意义(P<0.05)。结论基于Cox健康行为互动模式的护理措施在宫颈癌患者术后护理中的应用效果良好,能促进患者术后恢复,提高免疫功能和生活质量。

关键词：宫颈癌 Cox健康行为互动模式护理免疫功能生活质量